Chemical cytometry: ultrasensitive analysis of single cells
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Weinheim
Wiley-VCH
2010
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| Schlagworte: | |
| Online-Zugang: | Inhaltstext Inhaltsverzeichnis |
| Beschreibung: | Literaturangaben |
| Beschreibung: | XIX, 247 S. Ill., graph. Darst. |
| ISBN: | 9783527324958 |
Internformat
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Datensatz im Suchindex
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CONTENTS PREFACE XIU LIST OF CONTRIBUTORS XV 1 ORIGIN, CURRENT STATUS,
AND FUTURE PERSPECTIVES OF CHEMICAL CYTOMETRY 1 NORMAN J. DOVICHI 1.1
THE CELL AND CYTOMETRY 1 1.2 FLOW AND IMAGE CYTOMETRY 1 1.3 CHEMICAL
CYTOMETRY 2 1.3.1 PREHISTORY OF CHEMICAL CYTOMETRY - MICROCHEMISTRY,
MICROSPECTROSCOPY, AND MICROSEPARATIONS 3 1.3.2 EARLY HISTORY OF
CHEMICAL CYTOMETRY - BEFORE ULTRASENSITIVE DETECTION 4 1.3.3 PREHISTORY
OF CHEMICAL CYTOMETRY - THE ORIGINS OF CAPILLARY-BASED SEPARATIONS 5
1.3.4 PREHISTORY OF CHEMICAL CYTOMETRY - THE ORIGINS OF ULTRASENSITIVE
DETECTION 6 1.3.5 PREHISTORY OF CHEMICAL CYTOMETRY - THE POLYMERASE
CHAIN REACTION AND FLUORESCENCE-BASED DNA SEQUENCING 6 1.3.6 CHEMICAL
CYTOMETRY: PROTEIN ANALYSIS - THE FIRST EXPERIMENTS 7 1.3.7 CHEMICAL
CYTOMETRY: PROTEIN ANALYSIS * CELL LYSIS 7 1.3.8 CHEMICAL CYTOMETRY:
PROTEIN ANALYSIS - NATIVE FLUORESCENCE 8 1.3.9 CHEMICAL CYTOMETRY:
PROTEIN ANALYSIS - ON-COLUMN LABELING 9 1.3.10 CHEMICAL CYTOMETRY:
PROTEIN ANALYSIS - TWO-DIMENSIONAL CAPILLARY ELECTROPHORESIS 10 1.4
CHEMICAL CYTOMETRY OF DNA AND RNRNA 10 1.5 METABOLIC CYTOMETRY 10 1.5.
BIBLIOGRAFISCHE INFORMATIONEN HTTP://D-NB.INFO/996244913 DIGITALISIERT
DURCH VII CONTENTS 1.6.2 CHALLENGES - TISSUE DISSOCIATION CAN INTRODUCE
CHANGES IN A CELL'S COMPOSITION 12 1.6.3 CHALLENGES - CELLS NEED TO BE
FIXED TO PREVENT CHANGES ASSOCIATED WITH SAMPLE HANDLING 13 1.6.4
CHALLENGES - THE CONNECTION BETWEEN THE REAL WORLD AND THE WORLD OF
CHEMICAL CYTOMETRY 13 ACKNOWLEDGMENTS 14 REFERENCES 14 2 METABOLIC
CYTOMETRY - THE STUDY OF CLYCOSPHINGOLIPID METABOLISM IN SINGLE PRIMARY
CELLS OF THE DORSAL ROOT GANGLIA 21 COLIN D. WHITMORE, JULIAN
PRENDERGAST, DAVID ESSAKA, OLE HINDGAUL, MONICA P. PALDC, RONALD L.
SCHNAAR, AND NORMAN J. DOVICHI 2.1 INTRODUCTION 21 2.1.1
GLYCOSPHINGOLIPIDS PLAY AN IMPORTANT ROLE IN NEURONAL MEMBRANES 21 2.1.2
THE METABOLISM OF GLYCOSPHINGOLIPIDS IS QUITE COMPLEX 21 2.1.3
EXPRESSION OF GLYCOSPHINGOLIPIDS IS QUITE HETEROGENEOUS IN PRIMARY
TISSUES 22 2.1.4 METABOLIC CYTOMETRY HAS BEEN USED TO CHARACTERIZE
GLYCOSPHINGOLIPID METABOLISM IN MODEL CELL SYSTEMS 23 2.2 MATERIAL AND
METHODS 24 2.2.1 REAGENTS 24 2.2.2 CELLS AND CELL CULTURE 24 CONTENTS
VII 3.3 CELL PREPARATION AND REPORTER LOADING 35 3.3.1 DISRUPTIVE
LOADING METHODS 36 3.3.2 NONDISRUPTIVE LOADING METHODS 36 3.4 CELL LYSIS
AND SAMPLING TECHNIQUES 37 3.4.1 CHEMICAL LYSIS 37 3.4.2 HYPOTONIE LYSIS
39 3.4.3 LASER LYSIS 39 3.4.4 ELECTRICAL LYSIS 40 3.4.5 SAMPLING
TECHNIQUES 41 3.4.6 WHOLE CELL SAMPLING 41 3.4.7 SUBCELLULAR SAMPLING 42
3.5 ELECTROPHORESIS SEPARATION CONDITIONS 43 3.5.1 BUFFER EXCHANGE 43
3.5.2 NO BUFFER EXCHANGE 44 3.5.3 MICROCHANNEL ELECTROPHORETIC
SEPARATIONS 45 3.6 DETECTION 46 3.6.1 INSTRUMENTATION 46 3.6.1.1
EXCITATION 46 3.6.1.2 FIBER-OPTIC CABLE-BASED EXCITATION PATHWAY 48
3.6.1.3 EMISSION 48 3.6.1.4 DATA COLLECTION AND ANALYSIS 48 3.6.2 LIMIT
OF DETECTION (LOD) 49 3.6.3 DETECTION IN A MICROFLUIDIC DEVICE 49 3.7
AUTOMATION AND THROUGHPUT 50 VIH I CONTENTS 4.4.3.1 ELECTROPHORETIC
SEPARATION OF SYNECHOCOCCUS LYSATE 68 4.4.3.2 SYNECHOCOCCUS ANALYSIS
PROCEDURE 69 4.5 CONCLUSIONS 71 REFERENCES 71 5 CAPILLARY
ELECTROPHORESIS OF NUCLEIC ACIDS AT THE SINGLE-CELL LEVEL 75 NI LI AND
WENWAN THONG 5.1 INTRODUCTION 75 5.2 ON-LINE CELL ANALYSIS 76 5.2.1 CELL
INJECTION AND LYSIS 76 5.2.2 IN-COLUMN DNA OR RNA AMPLIFICATION WITH
INTEGRATED DEVICES 80 5.2.2.1 STREAM-LINED INSTRUMENTAL SETUP 80 5.2.2.2
OPTIMIZATION FOR REACTIONS 84 5.2.2.3 ANALYSIS OF AMPLIFIED PRODUCTS 87
5.3 DIRECT GENE AND GENE EXPRESSION ANALYSIS WITHOUT AMPLIFICATION 87
5.4 POTENTIAL ALTERNATIVE TECHNIQUES FOR SINGLE-CELL GENE AND GENE
EXPRESSION ANALYSIS 88 5.5 CONCLUSIONS 90 REFERENCES 90 6 MICROFLUIDIC
TECHNOLOGY FOR SINGLE-CELL ANALYSIS 93 YAN CHEN AND JIANG F. ZHONG 6.1
INTRODUCTION 93 6.1.1 LIMITATION OF CURRENT TECHNOLOGY 93 CONTENTS IX 7
ON-CHIP ELECTROPORATION AND ELECTROFUSION FOR SINGLE-CELL ENGINEERING
107 ANA VALERO AND ALBERT VAN DEN BERG 7.1 INTRODUCTION 107 7.2
SINGLE-CELL ELECTROPORATION IN MICROFLUIDIC DEVICES 108 7.2.1
MICRODEVICES FOR ANALYZING CELLULAR PROPERTIES OR INTRACELLULAR CONTENT
110 7.2.2 ELECTROPORATION MICRODEVICES FOR CELL INACTIVATION 112 7.2.3
ELECTROPORATION MICRODEVICES FOR GENE TRANSFECTION 113 7.3 SINGLE-CELL
ELECTROFUSION IN MICROFLUIDIC DEVICES 117 7.4 CONCLUSIONS 120 REFERENCES
121 8 ELECTROPORATIVE FLOW CYTOMETRY FOR SINGLE-CELL ANALYSIS 125 CHANG
LU,JUN WANG, NING BAO, AND HSIANG-YU WANG 8.1 INTRODUCTION 125 8.2
FLOW-THROUGH ELECTROPORATION UNDER CONSTANT VOLTAGE 126 8.3
ELECTROPORATIVE FLOW CYTOMETRY FOR DETECTING PROTEIN TRANSLOCATION 128
8.4 ELECTROPORATIVE FLOW CYTOMETRY FOR MEASURING SINGLE-CELL
BIOMECHANICS 134 8.5 ELECTROPORATIVE FLOW CYTOMETRY FOR SELECTIVELY
RELEASING AND ANALYZING SPECIFIC INTRACELLULAR MOLECULES 137 8.6
CONCLUSIONS 139 X| CONTENTS 10.4 MEASUREMENTS AT SINGLE CELLS 161 10.5
FUSION PORE DYNAMICS 163 10.5.1 STUDYING FUSION PORE IN LIVING CELLS 163
10.5.2 STUDYING FUSION PORE IN ARTIFICIAL CELLS 165 10.5.3 FLICKERING
FUSION PORE 367 10.6 CONCLUSIONS 170 ACKNOWLEDGMENTS 171 REFERENCES 372
11 ELECTROCHEMICAL DETERMINATION OF ENZYME ACTIVITY IN SINGLE CELLS 3 75
WENRUIJIN 11.1 INTRODUCTION 275 11.2 ELECTROCHEMICAL DETECTION COUPLED
WITH CAPILLARY ELECTROPHORESIS 175 11.2.1 DETERMINATION OF ACTIVITY OF
GLUCOSE-6-PHOSPHATE DEHYDROGENASE (G6PDH) IN SINGLE HUMAN ERYTHROCYTES
177 11.2.2 SEPARATION AND DETERMINATION OF ACTIVITY OF ALKALINE
PHOSPHATASE (ALP) ISOENZYMES IN SINGLE BALB/C FIBROLAST CELLS OF MOUSE
BONE MARROW 278 11.2.3 SEPARATION AND DETERMINATION OF ACTIVITY OF
LACTATE DEHYDROGENASE (LDH) ISOENZYMES IN SINGLE RAT GLIOMA CELLS 179
11.3 VOLTAMMETRY 280 11.4 SCANNING ELECTROCHEMICAL MICROSCOPY (SECM) 183
11.5 HIGH-THROUGHPUT ECD 188 11.6 PERSPECTIVE 290 ACKNOWLEDGMENT 190
REFERENCES 293 12 SINGLE-CELL MASS SPECTROMETRY 197 CONTENTS XI 13
OPTICAL SENSING ARRAYS FOR SINGLE-CELL ANALYSIS 229 RAGNHILD D. WHITAKER
AND DAVID R. WALT 13.1 INTRODUCTION TO FIBER-OPTIC SINGLE-CELL ARRAYS
219 13.2 ADVANTAGES OF FIBER-OPTIC SINGLE-CELL ARRAYS 220 13.3
FIBER-OPTIC ARRAYS 222 13.4 SINGLE-CELL ARRAYS FOR BACTERIA 223 13.4.1
ARRAY FABRICATION 223 13.4.2 LABELING AND DETECTION OF CELLULAR
RESPONSES IN BACTERIA ARRAYS 224 13.5 SINGLE-CELL ARRAYS FOR YEAST 226
13.5.1 ARRAY FABRICATION 226 13.5.2 LABELING AND DETECTION OF CELLULAR
RESPONSES IN YEAST ARRAYS 228 13.6 SINGLE-CELL ARRAYS FOR MAMMALIAN
CELLS 229 13.7 IMAGE AND DATA ANALYSIS FOR SINGLE-CELL ARRAYS 234 13.8
SUMMARY 234 REFERENCES 236 INDEX 239 |
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| spelling | Chemical cytometry ultrasensitive analysis of single cells ed. by Chang Lu Weinheim Wiley-VCH 2010 XIX, 247 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Literaturangaben Cytochemie (DE-588)4070172-4 gnd rswk-swf Einzelzellanalyse (DE-588)7657465-9 gnd rswk-swf Cytochemie (DE-588)4070172-4 s Einzelzellanalyse (DE-588)7657465-9 s DE-604 Lu, Chang (DE-588)127377417 edt text/html http://deposit.dnb.de/cgi-bin/dokserv?id=3348538&prov=M&dok_var=1&dok_ext=htm Inhaltstext DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020195269&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Chemical cytometry ultrasensitive analysis of single cells Cytochemie (DE-588)4070172-4 gnd Einzelzellanalyse (DE-588)7657465-9 gnd |
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| title | Chemical cytometry ultrasensitive analysis of single cells |
| title_auth | Chemical cytometry ultrasensitive analysis of single cells |
| title_exact_search | Chemical cytometry ultrasensitive analysis of single cells |
| title_full | Chemical cytometry ultrasensitive analysis of single cells ed. by Chang Lu |
| title_fullStr | Chemical cytometry ultrasensitive analysis of single cells ed. by Chang Lu |
| title_full_unstemmed | Chemical cytometry ultrasensitive analysis of single cells ed. by Chang Lu |
| title_short | Chemical cytometry |
| title_sort | chemical cytometry ultrasensitive analysis of single cells |
| title_sub | ultrasensitive analysis of single cells |
| topic | Cytochemie (DE-588)4070172-4 gnd Einzelzellanalyse (DE-588)7657465-9 gnd |
| topic_facet | Cytochemie Einzelzellanalyse |
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