Molecular regulation of Foxp3 expression: role and function of epigenetic modifications
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
Tönning [u.a.]
Der Andere Verl.
2009
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 105 S. Ill., graph. Darst. |
| ISBN: | 9783899599480 |
Internformat
MARC
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| 245 | 1 | 0 | |a Molecular regulation of Foxp3 expression |b role and function of epigenetic modifications |c Julia Katarina Polansky |
| 264 | 1 | |a Tönning [u.a.] |b Der Andere Verl. |c 2009 | |
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Datensatz im Suchindex
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|---|---|
| adam_text | I N D E X ZUSAMMENFASSUNG 9 SUMMARY 11 1. INTRODUCTION 13 1.1 THE
IMMUNOLOGICAL BALANCE: PATHOGEN DEFENSE VS. SELF-TOLERANCE ...13 1.2
TOLERANCE MECHANISMS 14 1.3 REGULATORY T CELLS 15 1.4 FOXP3*CD4 + TREGS
16 1.4.1 TREG GENERATION AND INDUCTION 17 1.4.2 TREG FUNCTION 18 1.4.3
THERAPEUTLC POTENTIAL OF TREGS 19 1.5 THE TRANSCRIPTION FACTOR FOXP3 19
1.5.1 ROLE OF FOXP3 IN TREG BIOLOGY 20 1.5.2 MOLECULAR FUNCTION OF FOXP3
21 1.5.3 REGULATION OF FOXP3 EXPRESSION 22 1.6 EPIGENETIC MECHANISMS IN
THE TRANSCRIPTIONAL CONTROL OF GENE EXPRESSION 24 1.6.1 MECHANISMS OF
EPIGENETIC GENE REGULATION 25 1.6.2 EPIGENETICS IN FOXP3 GENE REGULATION
27 1.7 AIMS OF THESTUDY 29 2. RESULTS 30 2.1 TGF-SS-MEDIATED FOXP3
EXPRESSION IN VITRO IS UNSTABLE AND DOES NOT LEAD TO TSDR DEMETHYLATION
30 2.2 DRUG-INDUCED TSDR DEMETHYLATION RESULTS IN STABLE FOXP3
EXPRESSION 34 2.3 IN VIVO CONVERTED TREG DISPLAY COMPLETE TSDR
DEMETHYLATION AND STABLE FOXP3 EXPRESSION 37 2.4 ONLY IN VIVO MATURED
FOXP3 + THYMOCYTES DISPLAY A DEMETHYLATED TSD BIBLIOGRAFISCHE
INFORMATIONEN HTTP://D-NB.INFO/999242016 DIGITALISIERT DURCH 8 3.
DISCUSSION 56 3.1 TSDR DEMETHYLATION DETERMINES THE STABILITY OF FOXP3
EXPRESSION - AS FAR AS STABILITY GOES 56 3.2 TSDR DEMETHYLATION OCCURS
LATE DURING THYMIC TREG-DEVELOPMENT - THROUGH AN ACTIVE DEMETHYLATION
MECHANISM? 62 3.3 THE DEMETHYLATED TSDR ACTS AS A TRANSCRIPTIONAL
ENHANCER FOR THE FOXP3 PROMOTER 64 3.4 POTENTIAL TSDR INTERACTION
PARTNERS: ETS-1 AND STAT-1 - BUT NOT FOXP3 ITSELF 67 3.5 CONCLUSIONS 72
4. MATERIAL AND METHODS 73 4.1 MICE 73 4.2 SURFACE AND INTRACELLULAR
STAINING PROCEDURES AND FLOW CYTOMETRY... 73 4.3 IN VIVO PROLIFERATION
ASSAY BY BRDU LABELING 74 4.4 ISOLATION OF MURINE EX VIVO T CELLS AND
THYMOCYTES 75 4.5 IN VITRO CULTURE OF T CELLS AND THYMOCYTES 77 4.6 IN
VIVO INDUCTION OF FOXP3 + TREGS BY DEC205 79 4.7 DNA-METHYLATION
ANALYSIS 79 4.8 CLONING OF LUCIFERASE-REPORTER PLASMIDS 79 4.8.1
FOXPRO-LUC AND TSDR-LUC 81 4.8.2 FOXPRO- AND TSDR-CONTAINING LUCIFERASE
VECTORS 81 4.8.3 TSDR-TRUNCATION MUTANTS AND TSDR-FRAGMENTS 82 4.8.4
DISRUPTION OF EBS1/2 AND CBS1/2 82 4.9 LUCIFERASE ASSAYS 82
4.10FUNCTIONAL INHIBITION OF TFS USING DSODNS 84 4.11
ELECTROMOBILITY-SHIFT ASSAY (EMSA) 85 4.12BIOINFORMATICS 87 5.
REFERENCES 88 6
|
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| author | Polansky, Julia Katarina 1979- |
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| dewey-ones | 571 - Physiology & related subjects |
| dewey-raw | 571.966 |
| dewey-search | 571.966 |
| dewey-sort | 3571.966 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| format | Thesis Book |
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| isbn | 9783899599480 |
| language | English |
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| physical | 105 S. Ill., graph. Darst. |
| publishDate | 2009 |
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| spelling | Polansky, Julia Katarina 1979- Verfasser (DE-588)140588329 aut Molecular regulation of Foxp3 expression role and function of epigenetic modifications Julia Katarina Polansky Tönning [u.a.] Der Andere Verl. 2009 105 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Zugl.: Berlin, Humboldt-Univ., Diss., 2009 Regulatorischer T-Lymphozyt (DE-588)1032038217 gnd rswk-swf Genregulation (DE-588)4122166-7 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Regulatorischer T-Lymphozyt (DE-588)1032038217 s Genregulation (DE-588)4122166-7 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020187989&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Polansky, Julia Katarina 1979- Molecular regulation of Foxp3 expression role and function of epigenetic modifications Regulatorischer T-Lymphozyt (DE-588)1032038217 gnd Genregulation (DE-588)4122166-7 gnd |
| subject_GND | (DE-588)1032038217 (DE-588)4122166-7 (DE-588)4113937-9 |
| title | Molecular regulation of Foxp3 expression role and function of epigenetic modifications |
| title_auth | Molecular regulation of Foxp3 expression role and function of epigenetic modifications |
| title_exact_search | Molecular regulation of Foxp3 expression role and function of epigenetic modifications |
| title_full | Molecular regulation of Foxp3 expression role and function of epigenetic modifications Julia Katarina Polansky |
| title_fullStr | Molecular regulation of Foxp3 expression role and function of epigenetic modifications Julia Katarina Polansky |
| title_full_unstemmed | Molecular regulation of Foxp3 expression role and function of epigenetic modifications Julia Katarina Polansky |
| title_short | Molecular regulation of Foxp3 expression |
| title_sort | molecular regulation of foxp3 expression role and function of epigenetic modifications |
| title_sub | role and function of epigenetic modifications |
| topic | Regulatorischer T-Lymphozyt (DE-588)1032038217 gnd Genregulation (DE-588)4122166-7 gnd |
| topic_facet | Regulatorischer T-Lymphozyt Genregulation Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=020187989&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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