Biomarkers in marine organisms: a practical approach
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| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Amsterdam [u.a.]
Elsevier
2001
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| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 550 S. graph. Darst. |
| ISBN: | 044482913X |
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| 245 | 1 | 0 | |a Biomarkers in marine organisms |b a practical approach |c ed. by Phillipe Garrigues ... |
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| 700 | 1 | |a Garrigues, Philippe |4 edt | |
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| adam_text | Biomarkers in Marine Organisms:
A Practical Approach
Edited by:
PHILIPPE GARRIGUES
Environmental and Toxicological Laboratory,
University of Bordeaux I/CNRS, France
HARTMUT BARTH
European Commission,
Brussels, Belgium
COLIN H WALKER
School of Animal and Microbial Sciences,
University of Reading, United Kingdom
JEAN FRANCOIS NARBONNE
Environmental and Toxicological Laboratory,
University of Bordeaux I/CNRS, France
ELSEVIER
Amsterdam - London - New York - Oxford - Paris - Shannon - Tokyo
CONTENTS
Preface v
Contents vii
List of Participants of European Projects xvii
Abbreviations xxi
Chapter 1 Induction of Molluscan Cytochrome P4S0 Monooxygenase
System as a Biomarker of Organic Pollution in Environmental
Monitoring 1
L D Peters and D R Livingstone
1 The uptake of xenobiotics and bioaccumulation in aquatic molluscs 2
2 Mixed function oxidase (MFO)-dependent metabolism 2
3 CYP1A as a biomonitoric tool 3
4 The molluscan MFO system: physical and catalytic properties 4
5 Multiple CYP forms in Mytilus sp 6
6 Regulation of CYP forms in Mytilus sp 7
7 Xenobiotic-dependent changes in Mytilus sp MFO properties: laboratory
studies 9
7 1 Putative induction of the CYPl-immunopositive protein 11
8 Xenobiotic-dependent changes in Mytilus sp MFO properties: field studies 14
8 1 Responses of M galloprovincialis CYP-immunopositive proteins in The
Venice Lagoon, Italy 15
8 2 Correlations between digestive gland microsomal CYP1 A-immunopositive
protein and BPH activity on Mytilus sp : recent studies 18
9 Final comments : 22
References 22
Chapter 2 Sensitivity and Specificity of Metallothionein as a Biomarker
for Aquatic Environment Biomonitoring 29
A Viarengo, B Burlando, V Evangelisti, S Mozzone and
F Dondero
1 Introduction 30
2 Materials and methods 31
3 Results and discussion : 32
References 39
Chapter 3 Genotoxicity Biomarkers in Aquatic Organisms as Indictors
of Carcinogenic Marine Pollutants 45
C Bolognesi and P Degan
1 Introduction 46
1 1 DNA damage 47
1 2 Chromosomal damage 48
2 Materials and methods 48
3 Results 51
4 Discussion 58
References 60
Chapter 4 DNA Adduct Detection in Mussels Exposed to Bulky Aromatic
Compounds in Laboratory and Field Conditions 65
P Venier
1 Introduction 66
1 1 Biological relevance of DNA adducts 66
1 2 The ^ -postlabelling assay 67
1 3 DNA adduct detection and environmental monitoring : 67
2 Formation and detection of DNA adducts in mussels 68
2 1 DNA adducts in mussels following laboratory exposure to genotoxins 69
2 2 DNA adducts in mussels following field exposure 70
3 Conclusion 77
References 78
Chapter 5 Developmental, Cytogenetic and Biochemical Effects of Spiked
or Environmentally Polluted Sediments in Sea Urchin
Bioassays 85
G Pagano, L G Korkina, M Iaccarino, A De Biase, I B Deeva,
Y K Doronin, M Guida G Melluso, S Merig, R Oral,
N M TrieffandM Warnau
1 Introduction 87
2 Materials and methods 88
3 Results 91
Conclusions 120
Appendix 122
References 128
Chapter 6 Comparative Study of Sediment and Mussel Aromatic
Compound Content in European Coastal Environments
Relationship with Specific Biomarkers 131
P Baumard, H Budzinski, P Garrigues, C Raoux, J Bellocq
and S Thompson
1 Introduction 133
2 Sediment PAH contamination 136
2 1 Sediment PAH analyses 136
2 2 Sediment total PAH concentration 137
2 3 Origin of the sediment contamination 138
2 4 Relationship between the origin and the extent of the sediment
contamination 144
2 5 Relationship between sediment PAH contamination and sediment
organic matter content 146
2 6 Grain-size PAH distribution 147
3 Mussel PAH contamination 150
3 1 Mussel PAH analysis 151
3 2 Mussel total PAH concentration 151
3 3 Relation between mussel and sediment total PAH concentration 152
3 4 Exposure of mussels to PAH contaminants present in the water column 155
4 Impact of sediment contamination on organisms 166
4 1 MFO activity in relation with sediment PAH contamination 166
4 2 Selective biotransformation capacity: case study of two isomeric
compounds, benzo[a]pyrene and benzo [e]pyrene 169
Conclusion 172
References 173
Chapter 7 Monitoring of Biological Effects of Pollutants: Field
Application 179
T Burgeot, G Bocquene, E His, F Vincent, O Geffard,
R Beiras, H Goraguer and F Galgani
1 Monitoring biological effects along French coasts 181
1 1 Introduction 181
1 2 EROD application in two pilot sites 183
1 3 Conclusion 189
2 Crassostrea Gigas Embryo and Larval Bioassays in Waters and Sediments 189
2 1 Introduction 189
2 2 Fresh and brackish water biomonitoring 191
2 3 Sediment biomonitoring with C gigas embryos and larvae 193
2 4 Conclusion 196
3 Testing biomarkers at sea: an in situ approach in the Northwestern
Mediterranean Sea 196
3 1 Introduction 196
3 2 Three biomarkers of exposure along the Mediterranean Sea 196
3 3 Conclusion 201
4 Molecular epidemiology studies using ras oncogene as a genetic marker of
malignancy for dragonet callionymus lyra along the French coast The Seine
estuary 203
4 1 Introduction 203
4 2 Dragonet exposure to PCBs and measurements of DNA adducts 204
4 3 Ras gene mutation analysis 205
4 4 Conclusion 206
5 Prospects 206
References 209
Chapter 8 Biochemical Markers in Mussel, Mytilus sp , and Pollution
Monitoring in European Coasts: Data Analysis 215
J F Narbonne, M Daubeze, P Baumard, H Budzinski,
C Clerandeau, F Akcha, P Mora and P Garrigues
1 Introduction 216
2 Materials and methods 217
2 1 Sample collection and preparation 217
2 2 Biochemical measurements 217
2 3 Chemical analysis 217
2 4 Statistical procedures 218
References 234
Chapter 9 Investigation of Genotoxicity and Immunotoxicity for
Monitoring Marine Pollution in the Baltic Sea and
Mediterranean Sea 237
H Dizer, E Unruh, V Bissinger andP -D Hansen
1 Introduction 239
2 Materials and methods 240
3 Results 244
4 Discussion 253
References 255
Chapter 10 Biochemical Tools for the Assessment of Pesticide
Exposure in a Deltaic Environment: The Use of
Cholinesterases and Carboxylesterases 259
C Porte, E Escartin and V Borghi
1 The Ebro delta: environmental threats 261
2 Toxicity of organophosphorus pesticides: inhibition of cholinesterases 262
3 The bivalve farms: Mytilus galloprovincialis 263
3 1 Characterisation of esterase activity 264
3 2 In vitro effects of pesticides 265
3 3 In vivo effects of pesticides 266
3 4 Seasonal variation in OPs pesticides body burden and esterase activity: a
field study , 267
3 5 Primary cell cultures: a valuable tool to evaluate toxicity and metabolism
of pesticides -270
3 6 Acetylcholinesterase reactivation techniques 271
3 7 Acetylcholinesterase activity in field collected specimens 274
References 275
Chapter 11 Environmental Monitoring in the North Sea by Combining
Biomarker Studies in the Sea Star Asterias Rubens with
Sediment Quality Assessment Based on Sea Urchin Bioassays 279
P J den Besten, J F Postma, S de Valk, M Dubbeldam and
J M Everaarts
1 Introduction 281
1 1 Use of echinoderm species in environmental monitoring 281
1 2 Previous research on impairment of reproduction by contaminants and
biomarker responses in the sea star A rubens 282
1 3 Sediment bioassays 284
1 4 Scope of the present research 284
2 Materials and methods 285
2 1 Field trips 285
2 2 Estimating reproductive success of sea starts 287
2 3 Biomarkers 287
2 4 Contaminant analysis in sediment and sea star tissues 289
2 5 Sediment bioassays 291
2 6 Statistical analyses 292
3 Results 293
3 1 Field sites 293
3 2 Contaminant levels in sea stars (both field trips)- 293
3 3 Relation between contaminant levels in sea starts and sediment 296
3 4 Measurements of reproductive success of sea stars (only first field trip) 297
3 5 Biomarker responses in sea starts 298
3 6 Sediment contamination and assessment of sediment quality with sea
urchin bioassays 302
4 Discussion 310
Appendix 319
References 326
Chapter 12 Cholinesterase Activity as a Bioindicator for Monitoring
Marine Pollution in the Baltic Sea and the Mediterranean
Sea 331
H Dizer, H C da Silva de Assis andP - D Hansen
1 Introduction 333
2 Materials and methods 333
3 Results 335
4 Discussion 339
References 340
Chapter 13 Evaluation of Various Biomarkers in the Wild Fish Serranus
Cabrilla Collected in the NW Mediterranean Sea 343
M Romeo, X Stien, M Gnassia-Barelli and M Lafaurie
1 Introduction 345
2 Materials and methods 346
2 1 Sampling cruise 346
2 2 Sample preparation for enzyme activities 347
2 3 Sample preparation for metal measurements 347
3 Results and discussion 348
References 355
Chapter 14 Microbiological Indicators for Monitoring Marine Pollution
in the Baltic Sea and the Mediterranean Sea 357
H Dizer and P -D Hansen •
1 Introduction 358
2 Materials and methods : 358
3 Results 360
4 Discussion 363
References 365
Chapter 15 Isolation of Cytochrome P450 CDNAS (CYP1A1 and
CYP4T2) from the Sea Bass (Dicentrarchus Labrax):
Tools for Biomonitoring Sea Pollution 367
C Sabourault, X Stien, J B Berge, J P Girard, M Lafaurie
and M Amichot
1 Introduction 369
2 Results and discussion 370
2 1 Molecular cloning of inducible CYP genes 370
2 2 Expression of CYP1 Al and CYP4T2 in untreated sea bass tissues 380
2 3 Induction of CYP1 Al and CYP4T2 by xenobiotics in sea bass tissues 383
References 390
Chapter 16 Inhibitory Effects of Heavy Metals on CYP1 Al Induction in
Black Seabream {Spondyliosoma Cantharus) Hepatocyte
Cultures 393
C Risso de Faverney, G de Sousa, M Lafaurie and R Rahmani
1 Introduction 395
• 2 Materials and methods 396
3 Results 398
References 406
Chapter 17 Biochemical Responses of Crabs (Carcinus spp) to Polycyclic
Aromatic Hydrocarbons (PAHs) as the Basis for New
Biomarker Assays 409
C H Walker and D Sawa
1 Introduction - ™ • 410
2 Induction of proteins 412
2 1 Screening for induction of proteins 413
2 2 Evidence for the induction of cytochrome P450 414
3 Genotoxicity 416
3 1 DNA fingerprinting 416
3 2 DNA fingerprinting using the PCR 417
3 3 DNA fingerprinting by AP-PCR as an alternative biomarker assay 418
3 4 Other assays for genotoxicity in crabs 421
4 Linked biomarker responses 422
5 Conclusions 423
References 426
Chapter 18 Cloning of Metallothionein cDNAS in Carcinus Maenas 431
D Sawa and B Li
1 Introduction 432
2 Experimental work 433
3 Results and discussion 433
4 Concluding remarks 436
References 437
Chapter 19 Development of Cytochrome P4S0 Biomarkers from
Posidinia Oceanica 439
A Schoendorf, I Benveniste andJ -P Salaiin
1 Introduction 440
2 Materals and methods 441
3 Results 443
4 Discussion 444
5 Future studies 445
References 445
Technical Annexes 447
Measurement of Total Cytochrome P450 Content in Digestive Gland
Microsomes of Mussel (Mytilus sp ) 449
D R Livingstone and L D Peters
Measurement of Cytochrome P450 Immunopositive Proteins in Digestive
Gland Microsomes of Mussel (Mytilus sp ) by Western Analysis 451
L D Peters and D R Livingstone
Metallothionein Assay 453
A Viarengo, B Burlando, V Evangelisti, S Mozzone and F Dondero
Evaluation of Lysosomal Membrane Stability 455
A Viarengo, B Burlando, V Evangelisti, S Mozzone and F Dondero
Alkaline Filter Elution Method 461
C Bolognesi and P Degan
Mussel Micronucleus Test 467
C Bolognesi and P Degan
Detection of Bulky Aromatic DNA Adducts by 32P-postlabelling 471
P Venier
Embryotoxicity on Sea Urchins 475
G Pagano, L G Korkina, M Iaccarino, A deBiase, I B Deeva, Y K Doronin,
M Guida, G Melluso, R Oral, N M TrieffandM Warnau
Sediment Spiking Technique 477
G Pagano, L G Korkina, M laccarino, A deBiase, I B Deeva, Y K Doronin,
M Guida, G Melluso, R Oral, N M TrieffandM Warnau
Measurement of Oxidative Activity: Luminol-dependent
Chemiluminescence and 8-Oxodesoxyguanine 478
G Pagano, L G Korkina, M laccarino, A de Biase, I B Deeva, Y K Doronin,
M Guida, G Melluso, R Oral, N M TrieffandM Warnau
Chemical Analyses of PAH in Sediment and Mussels 481
P Baumard, H Budzinski, P Garrigues, C Raoux, J Bellocq and S Thompson
Procedures for Cholinesterase Determination in Fish and Mussel 487
T Burgeot, G Bocquene, E His, F Vincent, 0 Geffard, R Beiras, F Quiniou
H Goraguer, F Galgani
Microplate Method for Measurement of EthoxyResorufin-O-Deethylase
(EROD) in Fish 491
T Burgeot, G Bocquene, E His, F Vincent, O Geffard, R Beiras, F Quiniou,
H Goraguer, F Galgani
Benzo(a)pyrene Hydroxylase Activity Measurement in Microsomes from
Mytilus sp 495
J F Narbonne, M Daubeze, P Baumard, H Budzinski, C Clerandeau,
F Akcha, P Mora and P Garrigues
Determination of Cholinesterase Activity in Mussel Gills 499
J F Narbonne, M Daubeze, P Baumard, H Budzinski, C Clerandeau,
F Akcha, P Mora and P Garrigues
Chemical Analysis: PAHs, PCBs and Pesticides 501
C Porte andD Barcelo
Biochemical Markers Acetylcholinesterase Activity and Cytochrome P450 503
C Porte and D Barcelo
Measurement of DNA-Strandbreaks in Tissue of the Sea Star Asterias
Rubens 505
P J den Besten, J F Postma, S de Valk, M Dubbeldam andJ M Everaarts
Measurement of Sediment-Toxicity Using a Fertilization and Embryonic
Development Assay with the Sea Urchins (Psammechinus MUiaris,
Paracentrotus Lividus) 509
P J den Besten, J F Postma, S de Valk M Dubbeldam andJ M Everaarts
Determination of EROD, GST and AchE Activities in Painted Comber
(Serranus CabrUla) 513
M Romeo, X Stien, M Gnassia-Barelli, M Lafaurie
Miniaturized DNA Unwinding Assay Employing Hydroxyapatite Batch
Elution 517
P Hansen and H Dizer
Molecular Cloning of CYP Genes in the Sea Bass (Dicentrarchus Labrax):
Experimental Procedures 519
C Sabourault, X Stien, J B Berge, J P Girard, M Lafaurie andM Amichot
Fish Hepatocyte Cultures 527
C Risso de Faverney, G de Sousa, M Lafaurie and R Rahmani
Determination of Cytochromes P4S0 Expression and Induction in Black
Seabream (Spondyliosama cantharus): Enzyme Activities, Northern and
Esters Blots 529
C Risso de Faverney, G de Sousa, M Lafaurie and R Rahmani
Biochemical Responses of Crabs (Carcinus spp) to Polycyclic Aromatic
Hydrocarbons (PAHs) as the Basis for New Biomarkers Assays 533
C H Walker and D Sawa
Cloning of Metallothionein cDNAs in Carcinus Maenas 537
D Sawa and B Li
Development of Biomarkers from Seagrass Posidonia Oceanica 539
A Schoendorf, I Benveniste andJ -P Salaiin
List of Contributors 543
Index of Keywords 549
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| spelling | Biomarkers in marine organisms a practical approach ed. by Phillipe Garrigues ... Amsterdam [u.a.] Elsevier 2001 550 S. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Garrigues, Philippe edt HEBIS Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=019911696&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Biomarkers in marine organisms a practical approach |
| title | Biomarkers in marine organisms a practical approach |
| title_auth | Biomarkers in marine organisms a practical approach |
| title_exact_search | Biomarkers in marine organisms a practical approach |
| title_full | Biomarkers in marine organisms a practical approach ed. by Phillipe Garrigues ... |
| title_fullStr | Biomarkers in marine organisms a practical approach ed. by Phillipe Garrigues ... |
| title_full_unstemmed | Biomarkers in marine organisms a practical approach ed. by Phillipe Garrigues ... |
| title_short | Biomarkers in marine organisms |
| title_sort | biomarkers in marine organisms a practical approach |
| title_sub | a practical approach |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=019911696&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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