Intracellular ribozyme applications: principles and protocols
Gespeichert in:
| Weitere Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Norfolk
Horizon Scientific Press
1999
|
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | X, 294 S. graph. Darst. |
| ISBN: | 1898486174 |
Internformat
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| 245 | 1 | 0 | |a Intracellular ribozyme applications |b principles and protocols |c ed. by John J. Rossi ... |
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Datensatz im Suchindex
| _version_ | 1804142316310495232 |
|---|---|
| adam_text | Contents
Contents iii
List of Contributors vi
Preface ix
Foreword x
Thomas R. Cech
Section 1. Selection
1. In Vitro Selection of Self-cleaving Ribozymes and Deoxyribozymes 1
Ronald R. Breaker
2. Developing Ribozymes for Therapeutic Applications 21
Through In Vitro Evolution
Roshan M. Kumar, and Gerald F. Joyce
3. Approaches for In Vivo Selection of Intracellularly 37
Active Ribozymes
Makiko Hamada, Hiroaki Kawasaki, Satoshi Fujita,
and Kazunari Taira
4. The Use of Cell Extracts and Antisense Deoxyribo-Oligonucleotides 47
for Identifying Ribozyme Cleavage Sites on Messenger RNAs
Michaela Scherr, Jeanne LeBon, Arthur D. Riggs, and John J. Rossi
Section 2. Kinetics
5. In Vitro Kinetic Evaluation of Hammerhead Ribozymes 57
Anton P. McCaffrey, and Olke C. Uhlenbeck
Section 3. Expression
6. Cytoplasmic Expression of Ribozyme Using a T7 Autogene System 69
Yuefeng Xie, Yunsheng Li, Kelly Walker, Qin Zhu, Wenlian Xu,
Thomas E. Wagner, and Xiaozhuo Chen
7. Expressing Active Ribozymes in Cells 79
Dmitry Samarsky, Gerardo Ferbeyre, and Edouard Bertrand
8. The Use of Small RNAs as Expression Cassettes for the 93
Delivery of Therapeutic RNAs
Cynthia P. Paul, Paul D. Good, and David R. Engelke
9. Targeted Ribozymes to Study Gene Function in Drosophila 103
Jack Jiagang Zhao, Carlos E. Vanario-Alonso, and Leslie Pick
10. Screening Promoters for Optimal Expression of Ribozymes 115
Richard Tritz, Mark Leavitt, and Jack R. Barber
Section 4. Delivery
11. Lipid-Based Carriers for the Delivery of Synthetic 125
Hammerhead Ribozymes
Mark A. Reynolds
12. Retroviral Vector Mediated Intracellular Delivery 139
of Ribozyme Genes
Ingrid Bahner
13. Adeno-associated Virus Vectors for Transduction of 189
Genes Encoding Ribozymes
Saswati Chatterjee, and K.K. Wong, Jr
Section 5. Detection
14. RNA Analysis by Terminal Transferase-Dependent PCR 217
Hsiu-Hua Chen, Daniela Castanotto, John J. Rossi,
and Arthur D. Riggs
Section 6. Pharmackodynamics
15. Ribozyme Pharmacokinetic Screening for Predicting 231
Pharmacodynamic Dosing Regimens
Tom J. Parry, Pamela A. Pavco, and Jennifer A. Sandberg
Section 7. Applications
16. Ribozymes for the Treatment of Restenosis following PTCA 243
Joan M. Robbins, Aaron Frimerman, Neal Eigler,
Frank Litvack, and Jack R. Barber
17. The Use of Ribozyme Gene Therapy for the Inhibition of 255
HIV Replication and its Pathogenic Sequelae
Justin E. Rigden, Julie A. Ely, Janet L. Macpherson,
Waxne L. Gerlach, Lun-Quan Sun, and Geoff P. Svmonds
18. Ribozyme Genes Protecting Transgenic Melon Plants 271
Against Potyviruses
Eric Huttnei; William Tucker, Agnes Vermeulen, Frederic Ignart,
Brett Sawyer, and Robert Birch
Index 285
|
| any_adam_object | 1 |
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| building | Verbundindex |
| bvnumber | BV025257056 |
| classification_rvk | WD 5355 |
| ctrlnum | (OCoLC)264567909 (DE-599)BVBBV025257056 |
| discipline | Biologie |
| format | Book |
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| illustrated | Illustrated |
| indexdate | 2024-07-09T22:29:50Z |
| institution | BVB |
| isbn | 1898486174 |
| language | English |
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| physical | X, 294 S. graph. Darst. |
| publishDate | 1999 |
| publishDateSearch | 1999 |
| publishDateSort | 1999 |
| publisher | Horizon Scientific Press |
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| spelling | Intracellular ribozyme applications principles and protocols ed. by John J. Rossi ... Norfolk Horizon Scientific Press 1999 X, 294 S. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Rossi, John J. edt HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=019893591&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Intracellular ribozyme applications principles and protocols |
| title | Intracellular ribozyme applications principles and protocols |
| title_auth | Intracellular ribozyme applications principles and protocols |
| title_exact_search | Intracellular ribozyme applications principles and protocols |
| title_full | Intracellular ribozyme applications principles and protocols ed. by John J. Rossi ... |
| title_fullStr | Intracellular ribozyme applications principles and protocols ed. by John J. Rossi ... |
| title_full_unstemmed | Intracellular ribozyme applications principles and protocols ed. by John J. Rossi ... |
| title_short | Intracellular ribozyme applications |
| title_sort | intracellular ribozyme applications principles and protocols |
| title_sub | principles and protocols |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=019893591&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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