An introduction to genetic engineering:
Saved in:
| Main Author: | |
|---|---|
| Format: | Book |
| Language: | English |
| Published: |
Cambridge <<[u.a.]>>
Cambridge University Press
2008
|
| Edition: | 3. ed. |
| Subjects: | |
| Online Access: | Inhaltsverzeichnis |
| Item Description: | Includes bibliographical references and index. - Previous ed.: 2002 |
| Physical Description: | XII, 336 S. Ill., graph. Darst. |
| ISBN: | 9780521615211 9780521850063 |
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MARC
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| 245 | 1 | 0 | |a An introduction to genetic engineering |c Desmond S. T. Nicholl |
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| 264 | 1 | |a Cambridge <<[u.a.]>> |b Cambridge University Press |c 2008 | |
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| _version_ | 1804140641071923200 |
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| adam_text | Titel: An introduction to genetic engineering
Autor: Nicholl, Desmond S. T
Jahr: 2008
Contents
Preface to the third edition page xi
Chapter I I Introduction 2
Chapter summary 2
1.1 What is genetic engineering? 3
1.2 Laying the foundations 5
1.3 First steps 6
1.4 What s in store? 1
Concept map 10
Part I I The basis of genetic engineering
Chapter 2 I Introducing molecular biology 12
Chapter summary 12
2.1 The way that living systems are organised 13
2.2 The flow of genetic information 15
2.3 The structure of DNA and RNA 16
2.4 Gene organisation 19
2.4.1 The anatomy of a gene 20
2.4.2 Gene structure in prokaryotes 21
2.4.3 Gene structure in eukaryotes 22
2.5 Gene expression 23
2.5.1 From genes to proteins 24
2.5.2 Transcription and translation 24
2.5.3 Regulation of gene expression 25
2.6 Genes and genomes 27
2.6.1 Genome size and complexity 28
2.6.2 Genome organisation 29
2.6.3 The transcriptome and proteome 30
Concept map 31
Chapter 3 I Working with nucleic acids 32
Chapter summary 32
3.1 Laboratory requirements 33
3.2 Isolation of DNA and RNA 34
3.3 Handling and quantification of nucleic acids 35
3.4 Labelling nucleic acids 37
3.4.1 Types of label - radioactive or not? 37
3.4.2 End labelling 38
3.4.3 Nick translation 38
3.4.4 Labelling by primer extension 39
3.5 Nucleic acid hybridisation 39
CONTENTS
3.6 Gel electrophoresis 40
3.7 DNA sequencing 41
3.7.1 Principles of DNA sequencing 42
3.7.2 Preparation of DNA fragments 43
3.7.3 Maxam-Gilbert (chemical) sequencing 44
3.7.4 Sanger-Coulson (dideoxy or enzymatic) sequencing 45
3.7.5 Electrophoresis and reading of sequences 47
3.7.6 Automation of DNA sequencing 48
Concept map 49
Chapter 4 i The tools of the trade so
Chapter summary 50
4.1 Restriction enzymes - cutting DNA 51
4.1.1 Type II restriction endonucleases 52
4.1.2 Use of restriction endonucleases 53
4.1.3 Restriction mapping 55
4.2 DNA modifying enzymes 55
4.2.1 Nucleases 56
4.2.2 Polymerases 57
4.2.3 Enzymes that modify the ends of DNA molecules 58
4.3 DNA ligase - joining DNA molecules 58
Concept map 60
Part III The methodology of gene manipulation
Chapter 5 I Host cells and vectors 62
Chapter summary 62
5.1 Host cell types 64
5.1.1 Prokaryotic hosts 64
5.1.2 Eukaryotic hosts 65
5.2 Plasmid vectors for use in £ coli 66
5.2.1 What are plasmids? 66
5.2.2 Basic cloning plasmids 67
5.2.3 Slightly more exotic plasmid vectors 69
5.3 Bacteriophage vectors for use in £ coli 70
5.3.1 What are bacteriophages? 71
5.3.2 Vectors based on bacteriophage 75
5.3.3 Vectors based on bacteriophage M13 78
5.4 Other vectors 79
5.4.1 Hybrid plasmid/phage vectors 80
5.4.2 Vectors for use in eukaryotic cells 80
5.4.3 Artificial chromosomes 83
5.5 Getting DNA into cells 84
5.5.1 Transformation and transfection 84
5.5.2 Packaging phage DNA in vitro 85
5.5.3 Alternative DNA delivery methods 86
Concept map gg
Chapter 6 I Cloning strategies 90
Chapter summary 90
6.1 Which approach is best? 91
6.2 Cloning from mRNA 93
6.2.1 Synthesis of cDNA 94
6.2.2 Cloning cDNA in plasmid vectors 97
6.2.3 Cloning cDNA in bacteriophage vectors 99
6.3 Cloning from genomic DNA 101
6.3.1 Genomic libraries 102
6.3.2 Preparation of DNA fragments for cloning 104
6.3.3 Ligation, packaging, and amplification of libraries 105
6.4 Advanced cloning strategies 108
6.4.1 Synthesis and cloning of cDNA 108
6.4.2 Expression of cloned DNA molecules 110
6.4.3 Cloning large DNA fragments in BAG and YAC vectors 113
Concept map lis
Chapter 7 I The polymerase chain reaction ii6
Chapter summary 116
7.1 History of the PCR 117
7.2 The methodology of the PCR 120
7.2.1 The essential features of the PCR 120
7.2.2 The design of primers for PCR 122
7.2.3 DNA polymerases for PCR 124
7.3 More exotic PCR techniques 125
7.3.1 PCR using mRNA templates 125
7.3.2 Nested PCR 126
7.3.3 Inverse PCR 127
7.3.4 RAPD and several other acronyms 127
7.4 Processing of PCR products 128
7.5 Applications of the PCR 130
Concept map 131
Chapter 8 I Selection, screening, and analysis of
recombinants 132
Chapter summary 132
8.1 Genetic selection and screening methods 134
8.1.1 The use of chromogenic substrates 134
8.1.2 Insertional inactivation 135
8.1.3 Complementation of defined mutations 137
8.1.4 Other genetic selection methods 137
8.2 Screening using nucleic acid hybridisation 138
8.2.1 Nucleic acid probes 138
8.2.2 Screening clone banks 140
8.3 Use of the PCR in screening protocols 142
8.4 Immunological screening for expressed genes 144
8.5 Analysis of cloned genes
8.5.1 Characterisation based on mRNA translation in vitro
8.5.2 Restriction mapping
8.5.3 Blotting techniques
8.5.4 DNA sequencing
Concept map
Chapter 9 I Bioinformatics 152
Chapter summary 152
9.1 What is bioinformatics? 153
9.2 The role of the computer 154
9.3 Biological data sets 156
9.3.1 Generation and organisation of information 157
9.3.2 Nucleic acid databases 157
9.3.3 Protein databases 160
9.4 Using bioinformatics as a tool 161
9.4.1 The impact of the Internet and the World Wide Web 162
9.4.2 Avoiding the GIGO effect - real experiments 163
9.4.3 Avoiding the test tube - computational experimentation 164
9.4.4 Presentation of database information 164
Concept map 167
Part III I Genetic engineering in action
Chapter 10 I Understanding genes, genomes,
and otheromes 170
170
Chapter summary
10.1 Analysis of gene structure and function 171
10.1.1 A closer look at sequences 172
10.1.2 Finding important regions of genes 172
174
10.1.3 Investigating gene expression
10.2 From genes to genomes 175
10.2.1 Gene expression in a genome context ^
10.2.2 Analysing genomes ^78
10.2.3 Mapping genomes
10.3 Genome sequencing 181
10.3.1 Sequencing technology ^
10.3.2 Genome projects ^88
10.4 The Human Genome Project 186
10.4.1 Whose genome, and how many genes does it contain? 168
10.4.2 Genetic and physical maps of the human genome I88
10.4.3 Deriving and assembling the sequence 1^0
10.4.4 Presentation and interrogation of the sequence 192
10.5 Otheromes 193
10.5.1 The transcriptome 194
10.5.2 The proteome 195
10.5.3 Metabolomes and interactomes 197
10.6 Life in the post-genomic era 198
Concept map 201
Chapter 11 I Genetic engineering and biotechnology 202
Chapter summary 202
11.1 Making proteins 204
11.1.1 Native and fusion proteins 204
11.1.2 Yeast expression systems 206
11.1.3 The baculovirus expression system 207
11.1.4 Mammalian cell lines 208
11.2 Protein engineering 208
11.2.1 Rational design 208
11.2.2 Directed evolution 211
11.3 From laboratory to production plant 211
11.3.1 Thinking big - the biotechnology industry 212
11.3.2 Production systems 214
11.3.3 Scale-up considerations 215
11.3.4 Downstream processing 215
11.4 Examples of biotechnological applications of rDNA
technology 216
11.4.1 Production of enzymes 216
11.4.2 The BST story 218
11.4.3 Therapeutic products for use in human healthcare 220
Concept map 225
Chapter 12 I Medical and forensic applications of gene
manipulation
Chapter summary
12.1 Diagnosis and characterisation of medical conditions
12.1.1 Diagnosis of infection
12.1.2 Patterns of inheritance
12.1.3 Genetically based disease conditions
12.2 Treatment using rDNA technology - gene therapy
12.2.1 Getting transgenes into patients
12.2.2 Gene therapy for adenosine deaminase deficiency
12.2.3 Gene therapy for cystic fibrosis
12.2.4 What does the future hold for gene therapy?
12.3 RNA interference - a recent discovery with great
potential
12.3.1 What is RNAi?
12.3.2 Using RNAi as a tool for studying gene expression
12.3.3 RNAi as a potential therapy
12.4 DNA profiling
12.4.1 The history of genetic fingerprinting
12.4.2 DNA profiling and the law
12.4.3 Mysteries of the past revealed by genetic detectives
Concept map
226
226
227
228
228
230
238
239
241
242
244
244
245
245
247
248
248
250
252
255
Chapter 13 I Transgenic plants and animals 256
Chapter summary 256
13.1 Transgenic plants 257
13.1.1 Why transgenic plants? 258
13.1.2 Ti plasmids as vectors for plant cells 258
13.1.3 Making transgenic plants 261
13.1.4 Putting the technology to work 262
13.2 Transgenic animals 269
13.2.1 Why transgenic animals? 270
13.2.2 Producing transgenic animals 270
13.2.3 Applications of transgenic animal technology 271
Concept map 277
Chapter 14 I The other sort of cloning 278
Chapter summary 278
14.1 Early thoughts and experiments 279
14.1.1 First steps towards cloning 280
14.1.2 Nuclear totipotency 281
14.2 Frogs and toads and carrots 283
14.3 A famous sheep - the breakthrough achieved 285
14.4 Beyond Dolly 287
Concept map 289
Chapter 15 I Brave new world or genetic nightmare? 291
15.1 Is science ethically and morally neutral? 291
15.2 Elements of the ethics debate 292
15.2.1 The role of the scientist 294
15.2.2 The role of society 294
15.3 Does Frankenstein s monster live inside Pandora s box? 295
Suggestions for further reading 297
Using the World Wide Web 299
Glossary 303
Index ,.,7
|
| any_adam_object | 1 |
| author | Nicholl, Desmond S. T. |
| author_facet | Nicholl, Desmond S. T. |
| author_role | aut |
| author_sort | Nicholl, Desmond S. T. |
| author_variant | d s t n dst dstn |
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| dewey-full | 660.65 |
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| dewey-ones | 660 - Chemical engineering |
| dewey-raw | 660.65 |
| dewey-search | 660.65 |
| dewey-sort | 3660.65 |
| dewey-tens | 660 - Chemical engineering |
| discipline | Chemie / Pharmazie Biologie |
| edition | 3. ed. |
| format | Book |
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| spelling | Nicholl, Desmond S. T. Verfasser aut An introduction to genetic engineering Desmond S. T. Nicholl 3. ed. Cambridge <<[u.a.]>> Cambridge University Press 2008 XII, 336 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Includes bibliographical references and index. - Previous ed.: 2002 Methode (DE-588)4038971-6 gnd rswk-swf Gentechnologie (DE-588)4071722-7 gnd rswk-swf Gentechnologie (DE-588)4071722-7 s Methode (DE-588)4038971-6 s 1\p DE-604 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=018592621&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
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| title | An introduction to genetic engineering |
| title_auth | An introduction to genetic engineering |
| title_exact_search | An introduction to genetic engineering |
| title_full | An introduction to genetic engineering Desmond S. T. Nicholl |
| title_fullStr | An introduction to genetic engineering Desmond S. T. Nicholl |
| title_full_unstemmed | An introduction to genetic engineering Desmond S. T. Nicholl |
| title_short | An introduction to genetic engineering |
| title_sort | an introduction to genetic engineering |
| topic | Methode (DE-588)4038971-6 gnd Gentechnologie (DE-588)4071722-7 gnd |
| topic_facet | Methode Gentechnologie |
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