Practical biotransformations: a beginner's guide
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Chichester
Wiley
2009
|
| Schriftenreihe: | Postgraduate Chemistry Series
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XII, 331 S. Ill., graph. Darst. |
| ISBN: | 9781405193672 |
Internformat
MARC
| LEADER | 00000nam a2200000zc 4500 | ||
|---|---|---|---|
| 001 | BV023748773 | ||
| 003 | DE-604 | ||
| 005 | 20110608 | ||
| 007 | t | ||
| 008 | 090310s2009 ad|| |||| 00||| eng d | ||
| 020 | |a 9781405193672 |9 978-1-405-19367-2 | ||
| 035 | |a (OCoLC)699010436 | ||
| 035 | |a (DE-599)BVBBV023748773 | ||
| 040 | |a DE-604 |b ger | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-526 |a DE-11 |a DE-M49 | ||
| 082 | 0 | |a 660.6 | |
| 084 | |a VS 5450 |0 (DE-625)147692:253 |2 rvk | ||
| 084 | |a CHE 810f |2 stub | ||
| 084 | |a CHE 825f |2 stub | ||
| 100 | 1 | |a Grogan, Gideon |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Practical biotransformations |b a beginner's guide |c Gideon Grogan |
| 264 | 1 | |a Chichester |b Wiley |c 2009 | |
| 300 | |a XII, 331 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 0 | |a Postgraduate Chemistry Series | |
| 650 | 0 | 7 | |a Biosynthese |0 (DE-588)4006902-3 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Biochemische Methode |0 (DE-588)4319880-6 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Enzymkatalyse |0 (DE-588)4152480-9 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Mikroorganismus |0 (DE-588)4039226-0 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Biokatalyse |0 (DE-588)4393622-2 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Transformation |g Genetik |0 (DE-588)4268828-0 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Biotransformation |0 (DE-588)4006908-4 |2 gnd |9 rswk-swf |
| 689 | 0 | 0 | |a Mikroorganismus |0 (DE-588)4039226-0 |D s |
| 689 | 0 | 1 | |a Enzymkatalyse |0 (DE-588)4152480-9 |D s |
| 689 | 0 | 2 | |a Biosynthese |0 (DE-588)4006902-3 |D s |
| 689 | 0 | 3 | |a Biotransformation |0 (DE-588)4006908-4 |D s |
| 689 | 0 | 4 | |a Biokatalyse |0 (DE-588)4393622-2 |D s |
| 689 | 0 | 5 | |a Transformation |g Genetik |0 (DE-588)4268828-0 |D s |
| 689 | 0 | 6 | |a Biochemische Methode |0 (DE-588)4319880-6 |D s |
| 689 | 0 | |C b |5 DE-604 | |
| 856 | 4 | 2 | |m OEBV Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017297848&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-017297848 | ||
Datensatz im Suchindex
| _version_ | 1804138799079358464 |
|---|---|
| adam_text | IMAGE 1
CONTENTS
FOREWORD IX
PREFACE XI
1 BIOTRANSFORMATIONS, MICROBES AND ENZYMES 1
1.1 INTRODUCTION 1
1.2 BIOTRANSFORMATIONS 8
1.3 MICROORGANISMS 8
1.4 ORGANISMNOMENCLATURE 14
1.5 ENZYMES 15
1.6 TYPES OFENZYMATICREACTIONS 18
1.7 ENZYMATIC COFACTORS 25
1.8 SOME BASIC CHARAETERISTICS OFENZYME CATALYSIS 32
1.9 TYPES OF BIOCATALYST - BIOTRANSFORMATIONS BY WHOIE CELLS OR
ISOLATED ENZYMES 38
1.10 CONCLUSION 40
REFERENCES 41
2 AN OVERVIEW OF BIOCATALYST SOURCES AND WEB-BASED INFORMATION 43
2.1 INTRODUCTION 43
2.2 MICROBIAL CULTURE COLLECTIONS 44
2.3 OBTAINING ORGANISMS FROM OTHER RESEARCH GROUPS 47
2.4 SELEETIVE ENRICHMENTS 47
2.5 METAGENOMICS 48
2.6 ENZYME SUPPLIERS AND BIOCATALYST DEVELOPMENT COMPANIES 50 2.7 GENOME
MINING FOR BIOCATALYSTS 54
2.8 OBTAINING AMINO ACID AND GENE SEQUENCE INFORMATION ON BIOCATALYSTS
55
2.9 OBTAINING DNA TEMPLATES FOR CLONING 66
2.10 CUSTOM GENE SYNTHESIS 67
2.11 OTHER WEB RESOURCES FOR FINDING INFORMATION ON BIOCATALYSTS 68
2.12 CONCLUSION 69
REFERENCES 70
V
IMAGE 2
VI CONTENTS
3 SETTING UP A LABORATORY FOR BIOTRANSFORMATIONS 71
3.1 INTRODUCTION 71
3.2 MICROBIOLOGICAL CONTAINMENT 72
3.3 ON CONTAINMENT ISSUES AND GENETICALLY MODIFIED ORGANISMS (GMOS) 75
3.4 EQUIPMENT FOR HANDLING MICROORGANISMS 77
3.5 TECHNIQUES AND TERMS IN MICROBIOLOGY - STERILITY, ASEPSIS AND
ASEPTIC TECHNIQUE 88
3.6 DISPOSAL OFVIABLE MICROBIAL WASTE AND DISINFECTION OF REUSABLE
EQUIPMENT 89
3.7 EQUIPMENT FOR ENZYMOLOGY AND MOLECUIAR BIOLOGY 91 3.8 GENERAL
REAGENTS AND CHEMICALS IN A BIOTRANSFORMATIONS LABORATORY 94
3.9 CONCLUSION 96
REFERENCES 97
4 A BEGINNER S GUIDE TO PREPARATIVE WHOLE-CELL MICROBIAL TRANSFORMATIONS
99
4.1 INTRODUCTION 99
4.2 STORAGE, MAINTENANCE AND GROWTH OFMICROORGANISMS 99 4.3 GENERAL
MICROBIOLOGICAL METHODS 103
4.4 EXAMPLES OFWHOLE-CELL BIOTRANSFORMATIONS USING BACTERIA 114 4.5
BIOTRANSFORMATION BY FILAMENTOUS FUNGI AND YEASTS 125 4.6 WHOLE-CELL
BIOTRANSFORMATIONS BY RECOMBINANT STRAINS OF E. COLI 134
4.7 CONCLUSION 145
REFERENCES 145
5 A BEGINNER S GUIDE TO BIOTRANSFORMATIONS CATALYSED BY
COMMERCIALLYAVAILABLE ISOLATED ENZYMES 147
5.1 INTRODUCTION 147
5.2 LIPASES 148
5.3 HYDROLYTIC REACTIONS USING LIPASES 151
5.4 USING LIPASES FOR ACYLATION REACTIONS 154
5.5 OTHER HYDROLASES 161
5.6 COMMERCIALLY AVAILABLE COENZYME-DEPENDENT ENZYMES 167 5.7
CARBON-CARBON BOND FORMING REACTIONS 178
5.8 CONCLUSION 182
REFERENCES 182
IMAGE 3
VII CONTENTS
6 A BEGINNER S GUIDE TO THE ISOLATION, ANALYSIS AND USE OFHOME-GROWN
ENZYME BIOCATALYSTS 185
6.1 INTRODUCTION 185
6.2 CELL GROWTH AND HARVESTING 186
6.3 CELL DISRUPTION 186
6.4 A TYPICAL PROCEDURE FOR MAKING A CELL EXTRACT FROM A RECOMBINANT
STRAIN OF E. CAU 190
6.5 PURIFICATION OFENZYMES - ABRIEF GUIDE 191
6.6 TECHNIQUES FOR PROTEIN PURIFICATION 195
6.7 ISOLATION OF RECOMBINANT ENZYMES USING HISTIDINE TAGS 201 6.8
ESTIMATION OF PROTEIN CONCENTRATION 203
6.9 CONCENTRATING PROTEIN SAMPIES BY CENTRIFUGATION 206 6.10 ANALYSIS OF
PROTEIN SAMPIES BY SODIUM DODECYLSULFATE POLYACYLAMIDE GEL
ELECTROPHORESIS 207
6.11 EXAMPLES OF ENZYME ASSAYS 212
6.12 USING HOME-GROWN ENZYMES FOR BIOTRANSFORMATIONS SOME RECENT
EXAMPLES 224
6.13 CONCLUSION 232
REFERENCES 233
7 AN INTRODUCTION TO BASIC GENE CLONING FOR THE PRODUCTION OFDESIGNER
BIOCATALYSTS 235
7.1 INTRODUCTION 235
7.2 BACKGROUND 238
7.3 GENE AMPLIFICATION BY POLYMERASE CHAIN REACTION (PCR) 249 7.4 DNA
FRAGMENT ANALYSIS BY AGAROSE GEL ELECTROPHORESIS 256 7.5 GENE CLONING
260
7.6 ANALYSIS BY DNA SEQUENCING 266
7.7 TROUBLESHOOTING THE GENE AMPLIFICATION A ND CLONING PROCESS 267
7.8 LIGATION-INDEPENDENT CLONING 268
7.9 GENE EXPRESSION 270
7.10 CONCLUSION 276
REFERENCES 277
8 ENGINEERING ENZYMES 279
8.1 INTRODUCTION 279
8.2 SITE-DIRECTED OR TARGETED MUTAGENESIS AS A TOOL FOR INVESTIGATING
ENZYME MECHANISM OR ALTERING CATALYTIC ATTRIBUTES 280
IMAGE 4
VIII CONTENTS
8.3 ENGINEERING USING RANDOM MUTAGENESIS DIRECTED EVOLUTION OFENES 291
8.4 COMBINING RATIONAL AND RANDOM MUTAGENESIS FOR BIOCATALYSTLMPROVEMENT
300
8.5 EXPLOITING CATALYTIC PROMISCUITY FOR CREATING NEW ENE ACTIVITIES
303
8.6 DESIGNING ENES IN SILICO 304
8.7 CONDUSION 305
REFERENCES 305
APPENDICES 309
INDEX 323
|
| any_adam_object | 1 |
| author | Grogan, Gideon |
| author_facet | Grogan, Gideon |
| author_role | aut |
| author_sort | Grogan, Gideon |
| author_variant | g g gg |
| building | Verbundindex |
| bvnumber | BV023748773 |
| classification_rvk | VS 5450 |
| classification_tum | CHE 810f CHE 825f |
| ctrlnum | (OCoLC)699010436 (DE-599)BVBBV023748773 |
| dewey-full | 660.6 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 660 - Chemical engineering |
| dewey-raw | 660.6 |
| dewey-search | 660.6 |
| dewey-sort | 3660.6 |
| dewey-tens | 660 - Chemical engineering |
| discipline | Chemie / Pharmazie Chemie |
| format | Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>02033nam a2200505zc 4500</leader><controlfield tag="001">BV023748773</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20110608 </controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">090310s2009 ad|| |||| 00||| eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9781405193672</subfield><subfield code="9">978-1-405-19367-2</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)699010436</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV023748773</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-526</subfield><subfield code="a">DE-11</subfield><subfield code="a">DE-M49</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">660.6</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">VS 5450</subfield><subfield code="0">(DE-625)147692:253</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">CHE 810f</subfield><subfield code="2">stub</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">CHE 825f</subfield><subfield code="2">stub</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Grogan, Gideon</subfield><subfield code="e">Verfasser</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Practical biotransformations</subfield><subfield code="b">a beginner's guide</subfield><subfield code="c">Gideon Grogan</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Chichester</subfield><subfield code="b">Wiley</subfield><subfield code="c">2009</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">XII, 331 S.</subfield><subfield code="b">Ill., graph. Darst.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="0" ind2=" "><subfield code="a">Postgraduate Chemistry Series</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Biosynthese</subfield><subfield code="0">(DE-588)4006902-3</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Biochemische Methode</subfield><subfield code="0">(DE-588)4319880-6</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Enzymkatalyse</subfield><subfield code="0">(DE-588)4152480-9</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Mikroorganismus</subfield><subfield code="0">(DE-588)4039226-0</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Biokatalyse</subfield><subfield code="0">(DE-588)4393622-2</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Transformation</subfield><subfield code="g">Genetik</subfield><subfield code="0">(DE-588)4268828-0</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Biotransformation</subfield><subfield code="0">(DE-588)4006908-4</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Mikroorganismus</subfield><subfield code="0">(DE-588)4039226-0</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Enzymkatalyse</subfield><subfield code="0">(DE-588)4152480-9</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="2"><subfield code="a">Biosynthese</subfield><subfield code="0">(DE-588)4006902-3</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="3"><subfield code="a">Biotransformation</subfield><subfield code="0">(DE-588)4006908-4</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="4"><subfield code="a">Biokatalyse</subfield><subfield code="0">(DE-588)4393622-2</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="5"><subfield code="a">Transformation</subfield><subfield code="g">Genetik</subfield><subfield code="0">(DE-588)4268828-0</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="6"><subfield code="a">Biochemische Methode</subfield><subfield code="0">(DE-588)4319880-6</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="C">b</subfield><subfield code="5">DE-604</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">OEBV Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017297848&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-017297848</subfield></datafield></record></collection> |
| id | DE-604.BV023748773 |
| illustrated | Illustrated |
| indexdate | 2024-07-09T21:33:55Z |
| institution | BVB |
| isbn | 9781405193672 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-017297848 |
| oclc_num | 699010436 |
| open_access_boolean | |
| owner | DE-526 DE-11 DE-M49 DE-BY-TUM |
| owner_facet | DE-526 DE-11 DE-M49 DE-BY-TUM |
| physical | XII, 331 S. Ill., graph. Darst. |
| publishDate | 2009 |
| publishDateSearch | 2009 |
| publishDateSort | 2009 |
| publisher | Wiley |
| record_format | marc |
| series2 | Postgraduate Chemistry Series |
| spelling | Grogan, Gideon Verfasser aut Practical biotransformations a beginner's guide Gideon Grogan Chichester Wiley 2009 XII, 331 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Postgraduate Chemistry Series Biosynthese (DE-588)4006902-3 gnd rswk-swf Biochemische Methode (DE-588)4319880-6 gnd rswk-swf Enzymkatalyse (DE-588)4152480-9 gnd rswk-swf Mikroorganismus (DE-588)4039226-0 gnd rswk-swf Biokatalyse (DE-588)4393622-2 gnd rswk-swf Transformation Genetik (DE-588)4268828-0 gnd rswk-swf Biotransformation (DE-588)4006908-4 gnd rswk-swf Mikroorganismus (DE-588)4039226-0 s Enzymkatalyse (DE-588)4152480-9 s Biosynthese (DE-588)4006902-3 s Biotransformation (DE-588)4006908-4 s Biokatalyse (DE-588)4393622-2 s Transformation Genetik (DE-588)4268828-0 s Biochemische Methode (DE-588)4319880-6 s b DE-604 OEBV Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017297848&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Grogan, Gideon Practical biotransformations a beginner's guide Biosynthese (DE-588)4006902-3 gnd Biochemische Methode (DE-588)4319880-6 gnd Enzymkatalyse (DE-588)4152480-9 gnd Mikroorganismus (DE-588)4039226-0 gnd Biokatalyse (DE-588)4393622-2 gnd Transformation Genetik (DE-588)4268828-0 gnd Biotransformation (DE-588)4006908-4 gnd |
| subject_GND | (DE-588)4006902-3 (DE-588)4319880-6 (DE-588)4152480-9 (DE-588)4039226-0 (DE-588)4393622-2 (DE-588)4268828-0 (DE-588)4006908-4 |
| title | Practical biotransformations a beginner's guide |
| title_auth | Practical biotransformations a beginner's guide |
| title_exact_search | Practical biotransformations a beginner's guide |
| title_full | Practical biotransformations a beginner's guide Gideon Grogan |
| title_fullStr | Practical biotransformations a beginner's guide Gideon Grogan |
| title_full_unstemmed | Practical biotransformations a beginner's guide Gideon Grogan |
| title_short | Practical biotransformations |
| title_sort | practical biotransformations a beginner s guide |
| title_sub | a beginner's guide |
| topic | Biosynthese (DE-588)4006902-3 gnd Biochemische Methode (DE-588)4319880-6 gnd Enzymkatalyse (DE-588)4152480-9 gnd Mikroorganismus (DE-588)4039226-0 gnd Biokatalyse (DE-588)4393622-2 gnd Transformation Genetik (DE-588)4268828-0 gnd Biotransformation (DE-588)4006908-4 gnd |
| topic_facet | Biosynthese Biochemische Methode Enzymkatalyse Mikroorganismus Biokatalyse Transformation Genetik Biotransformation |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=017297848&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT grogangideon practicalbiotransformationsabeginnersguide |