The Role of Wnt signalling in the development of somites and neural crest:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Berlin [u.a.]
Springer
2008
|
| Schriftenreihe: | Advances in anatomy, embryology and cell biology
195 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | X, 64 S. Ill, graph. Darst. |
| ISBN: | 3540777261 9783540777267 |
Internformat
MARC
| LEADER | 00000nam a2200000 cb4500 | ||
|---|---|---|---|
| 001 | BV023355037 | ||
| 003 | DE-604 | ||
| 005 | 20080704 | ||
| 007 | t | ||
| 008 | 080620s2008 gw ad|| |||| 00||| eng d | ||
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| 016 | 7 | |a 986861626 |2 DE-101 | |
| 020 | |a 3540777261 |c Pb. : ca. EUR 90.90 (freier Pr.), ca. sfr 148.00 (freier Pr.) |9 3-540-77726-1 | ||
| 020 | |a 9783540777267 |c Pb. : ca. EUR 90.90 (freier Pr.), ca. sfr 148.00 (freier Pr.) |9 978-3-540-77726-7 | ||
| 028 | 5 | 2 | |a 12214341 |
| 035 | |a (OCoLC)213384263 | ||
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| 040 | |a DE-604 |b ger |e rakddb | ||
| 041 | 0 | |a eng | |
| 044 | |a gw |c XA-DE-BE | ||
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| 084 | |a 610 |2 sdnb | ||
| 245 | 1 | 0 | |a The Role of Wnt signalling in the development of somites and neural crest |c C. Schmidt ... |
| 264 | 1 | |a Berlin [u.a.] |b Springer |c 2008 | |
| 300 | |a X, 64 S. |b Ill, graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Advances in anatomy, embryology and cell biology |v 195 | |
| 650 | 4 | |a Neural Crest |x physiology | |
| 650 | 4 | |a Neural crest | |
| 650 | 4 | |a Somite | |
| 650 | 4 | |a Somites |x physiology | |
| 650 | 4 | |a Wnt Proteins |x physiology | |
| 650 | 4 | |a Wnt proteins | |
| 650 | 0 | 7 | |a Wnt-Proteine |0 (DE-588)7515809-7 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Embryonalentwicklung |0 (DE-588)4070792-1 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Neuralleiste |0 (DE-588)4503267-1 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Signaltransduktion |0 (DE-588)4318717-1 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Somit |0 (DE-588)4409995-2 |2 gnd |9 rswk-swf |
| 689 | 0 | 0 | |a Neuralleiste |0 (DE-588)4503267-1 |D s |
| 689 | 0 | 1 | |a Embryonalentwicklung |0 (DE-588)4070792-1 |D s |
| 689 | 0 | 2 | |a Wnt-Proteine |0 (DE-588)7515809-7 |D s |
| 689 | 0 | 3 | |a Signaltransduktion |0 (DE-588)4318717-1 |D s |
| 689 | 0 | |5 DE-604 | |
| 689 | 1 | 0 | |a Somit |0 (DE-588)4409995-2 |D s |
| 689 | 1 | 1 | |a Embryonalentwicklung |0 (DE-588)4070792-1 |D s |
| 689 | 1 | 2 | |a Wnt-Proteine |0 (DE-588)7515809-7 |D s |
| 689 | 1 | 3 | |a Signaltransduktion |0 (DE-588)4318717-1 |D s |
| 689 | 1 | |5 DE-604 | |
| 700 | 1 | |a Schmidt, Corina |d 1969- |e Sonstige |0 (DE-588)122243447 |4 oth | |
| 776 | 0 | 8 | |i Erscheint auch als |n Online-Ausgabe |z 978-3-540-77727-4 |
| 830 | 0 | |a Advances in anatomy, embryology and cell biology |v 195 |w (DE-604)BV002528506 |9 195 | |
| 856 | 4 | 2 | |m Digitalisierung UB Regensburg |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016538585&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-016538585 | ||
Datensatz im Suchindex
| _version_ | 1804137714715459584 |
|---|---|
| adam_text | List of
Contents
1
Introduction
........................................................ 1
1.1
Wnts and Development
............................................... 1
1.2
Somite Formation
.................................................... 6
1.3
Induction, Delamination and Migration of Neural Crest
................... 9
1.3.1
Induction of the Neural Crest
.......................................... 10
1.3.2
Delamination and Migration of the Neural Crest
......................... 12
1.4
Outline of Research Plan
.............................................. 14
1.4.1
Wnt6 and Somitogenesis
.............................................. 14
1.4.2
The Regulation of cNkdl Expression
.................................... 14
1.4.3
Wnt6 and Neural Crest
................................................ 15
2
Materials and Methods
............................................... 15
2.1
Embryos
............................................................ 15
2.2
Culture of Wnt-Expressing Cells
....................................... 15
2.3
Operations
.......................................................... 16
2.4
BrdU Experiment
.................................................... 17
2.5
Neural Crest Cell Culture
.............................................. 17
2.6
HNK
Staining Neural Crest Culture
..................................... 17
2.7
Wnt6 siRNA RCAS Construct
.......................................... ¡7
2.8
Immunohistochemistry on Sections
....................................
!8
2.9
Immunohistochemistry on Whole Embryos
............................. 18
2.10
Whoie-Mount in Situ Hybridisation
.................................... 19
2.11
Chemicals
........................................................... 19
2.12
Anti-sense
RNA
Probes
............................................... 19
2.12.1 DNA
Linearisation
................................................... 20
2.12.2 DNA
Purification
.................................................... 20
2.12.3
Probe Synthesis
...................................................... 21
3
Results
............................................................. 21
3.1
Wnt6 and Somitogenesis
.............................................. 21
3.1.1
Consequences of Ectoderm Removal on the Epithelialisation
of
Segmental
Plate
.................................................... 21
3.1.2
Effect of Vnt6 on the Development of Somites
........................... 23
3.1.3
Wntó
Rescues the Effect of Ectoderm Removal
........................... 23
3.1.4
Wnt6 Rescues the Effect of Medial Barrier Insertion
...................... 25
3.1.5
Effect of Wnt6 Expression on Muscle Development
....................... 28
3.1.6
Effect of Sfrp2 on Somite Development
.................................. 29
List of Contents
3.2
The Regulation of cNkdl Expression
.................................... 29
3.2.1
Normal Expression of cNkdl in Chicken Embryos
ѓготНШ-Нто
.................................................... 29
3.2.2
Regulation of cNkdl Expression
........................................ 31
3.2.3
Regulation of cNkdl by Members of the Wnt Family
...................... 33
3.3
Wnt6 and Neural Crest
................................................ 36
3.3.1
Wnt6 Induces Neural Crest Production and Wntl Inhibits
Neural Crest Induction
................................................ 36
3.3.2
Wnt6 Induces Neural Crest Production Through the Non-canonical
Signalling Pathway
................................................... 37
3.3.3
Activation of the Non-canonical Pathway Through Dsh Induces
Neural Crest
......................................................... 37
3.3.4
The Canonical Wnt Pathway Inhibits Neural Crest Induction
............... 39
3.3.5
Specific Inhibition of Wnt6 Reduces Neural Crest Production
.............. 41
4
Discussion
.......................................................... 43
4.1
Wnt6 and Somitogenesis
.............................................. 43
4.2
The Regulation of cNkdl Expression
.................................... 47
4.3
Wnts and Neural Crest Induction
....................................... 49
References
................................................................. 53
|
| adam_txt |
List of
Contents
1
Introduction
. 1
1.1
Wnts and Development
. 1
1.2
Somite Formation
. 6
1.3
Induction, Delamination and Migration of Neural Crest
. 9
1.3.1
Induction of the Neural Crest
. 10
1.3.2
Delamination and Migration of the Neural Crest
. 12
1.4
Outline of Research Plan
. 14
1.4.1
Wnt6 and Somitogenesis
. 14
1.4.2
The Regulation of cNkdl Expression
. 14
1.4.3
Wnt6 and Neural Crest
. 15
2
Materials and Methods
. 15
2.1
Embryos
. 15
2.2
Culture of Wnt-Expressing Cells
. 15
2.3
Operations
. 16
2.4
BrdU Experiment
. 17
2.5
Neural Crest Cell Culture
. 17
2.6
HNK
Staining Neural Crest Culture
. 17
2.7
Wnt6 siRNA RCAS Construct
. ¡7
2.8
Immunohistochemistry on Sections
.
!8
2.9
Immunohistochemistry on Whole Embryos
. 18
2.10
Whoie-Mount in Situ Hybridisation
. 19
2.11
Chemicals
. 19
2.12
Anti-sense
RNA
Probes
. 19
2.12.1 DNA
Linearisation
. 20
2.12.2 DNA
Purification
. 20
2.12.3
Probe Synthesis
. 21
3
Results
. 21
3.1
Wnt6 and Somitogenesis
. 21
3.1.1
Consequences of Ectoderm Removal on the Epithelialisation
of
Segmental
Plate
. 21
3.1.2
Effect of \Vnt6 on the Development of Somites
. 23
3.1.3
Wntó
Rescues the Effect of Ectoderm Removal
. 23
3.1.4
Wnt6 Rescues the Effect of Medial Barrier Insertion
. 25
3.1.5
Effect of Wnt6 Expression on Muscle Development
. 28
3.1.6
Effect of Sfrp2 on Somite Development
. 29
List of Contents
3.2
The Regulation of cNkdl Expression
. 29
3.2.1
Normal Expression of cNkdl in Chicken Embryos
ѓготНШ-Нто
. 29
3.2.2
Regulation of cNkdl Expression
. 31
3.2.3
Regulation of cNkdl by Members of the Wnt Family
. 33
3.3
Wnt6 and Neural Crest
. 36
3.3.1
Wnt6 Induces Neural Crest Production and Wntl Inhibits
Neural Crest Induction
. 36
3.3.2
Wnt6 Induces Neural Crest Production Through the Non-canonical
Signalling Pathway
. 37
3.3.3
Activation of the Non-canonical Pathway Through Dsh Induces
Neural Crest
. 37
3.3.4
The Canonical Wnt Pathway Inhibits Neural Crest Induction
. 39
3.3.5
Specific Inhibition of Wnt6 Reduces Neural Crest Production
. 41
4
Discussion
. 43
4.1
Wnt6 and Somitogenesis
. 43
4.2
The Regulation of cNkdl Expression
. 47
4.3
Wnts and Neural Crest Induction
. 49
References
. 53 |
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| id | DE-604.BV023355037 |
| illustrated | Illustrated |
| index_date | 2024-07-02T21:06:41Z |
| indexdate | 2024-07-09T21:16:41Z |
| institution | BVB |
| isbn | 3540777261 9783540777267 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-016538585 |
| oclc_num | 213384263 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR |
| owner_facet | DE-355 DE-BY-UBR |
| physical | X, 64 S. Ill, graph. Darst. |
| publishDate | 2008 |
| publishDateSearch | 2008 |
| publishDateSort | 2008 |
| publisher | Springer |
| record_format | marc |
| series | Advances in anatomy, embryology and cell biology |
| series2 | Advances in anatomy, embryology and cell biology |
| spelling | The Role of Wnt signalling in the development of somites and neural crest C. Schmidt ... Berlin [u.a.] Springer 2008 X, 64 S. Ill, graph. Darst. txt rdacontent n rdamedia nc rdacarrier Advances in anatomy, embryology and cell biology 195 Neural Crest physiology Neural crest Somite Somites physiology Wnt Proteins physiology Wnt proteins Wnt-Proteine (DE-588)7515809-7 gnd rswk-swf Embryonalentwicklung (DE-588)4070792-1 gnd rswk-swf Neuralleiste (DE-588)4503267-1 gnd rswk-swf Signaltransduktion (DE-588)4318717-1 gnd rswk-swf Somit (DE-588)4409995-2 gnd rswk-swf Neuralleiste (DE-588)4503267-1 s Embryonalentwicklung (DE-588)4070792-1 s Wnt-Proteine (DE-588)7515809-7 s Signaltransduktion (DE-588)4318717-1 s DE-604 Somit (DE-588)4409995-2 s Schmidt, Corina 1969- Sonstige (DE-588)122243447 oth Erscheint auch als Online-Ausgabe 978-3-540-77727-4 Advances in anatomy, embryology and cell biology 195 (DE-604)BV002528506 195 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016538585&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | The Role of Wnt signalling in the development of somites and neural crest Advances in anatomy, embryology and cell biology Neural Crest physiology Neural crest Somite Somites physiology Wnt Proteins physiology Wnt proteins Wnt-Proteine (DE-588)7515809-7 gnd Embryonalentwicklung (DE-588)4070792-1 gnd Neuralleiste (DE-588)4503267-1 gnd Signaltransduktion (DE-588)4318717-1 gnd Somit (DE-588)4409995-2 gnd |
| subject_GND | (DE-588)7515809-7 (DE-588)4070792-1 (DE-588)4503267-1 (DE-588)4318717-1 (DE-588)4409995-2 |
| title | The Role of Wnt signalling in the development of somites and neural crest |
| title_auth | The Role of Wnt signalling in the development of somites and neural crest |
| title_exact_search | The Role of Wnt signalling in the development of somites and neural crest |
| title_exact_search_txtP | The Role of Wnt signalling in the development of somites and neural crest |
| title_full | The Role of Wnt signalling in the development of somites and neural crest C. Schmidt ... |
| title_fullStr | The Role of Wnt signalling in the development of somites and neural crest C. Schmidt ... |
| title_full_unstemmed | The Role of Wnt signalling in the development of somites and neural crest C. Schmidt ... |
| title_short | The Role of Wnt signalling in the development of somites and neural crest |
| title_sort | the role of wnt signalling in the development of somites and neural crest |
| topic | Neural Crest physiology Neural crest Somite Somites physiology Wnt Proteins physiology Wnt proteins Wnt-Proteine (DE-588)7515809-7 gnd Embryonalentwicklung (DE-588)4070792-1 gnd Neuralleiste (DE-588)4503267-1 gnd Signaltransduktion (DE-588)4318717-1 gnd Somit (DE-588)4409995-2 gnd |
| topic_facet | Neural Crest physiology Neural crest Somite Somites physiology Wnt Proteins physiology Wnt proteins Wnt-Proteine Embryonalentwicklung Neuralleiste Signaltransduktion Somit |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016538585&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV002528506 |
| work_keys_str_mv | AT schmidtcorina theroleofwntsignallinginthedevelopmentofsomitesandneuralcrest |