The nucleus: 2 Chromatin, transcription, envelope, proteins, dynamics, and imaging
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2008
|
| Schriftenreihe: | Methods in molecular biology
464 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XVIII, 444 S. Ill., graph. Darst. |
| ISBN: | 9781603274609 9781603274616 |
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| 245 | 1 | 0 | |a The nucleus |n 2 |p Chromatin, transcription, envelope, proteins, dynamics, and imaging |c Ronald Hancock, ed. |
| 246 | 1 | 3 | |a Physical properties and imaging methods |
| 264 | 1 | |a Totowa, NJ |b Humana Press |c 2008 | |
| 300 | |a XVIII, 444 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in molecular biology |v 464 | |
| 490 | 0 | |a Methods in molecular biology |v ... | |
| 650 | 7 | |a Noyau cellulaire |2 ram | |
| 650 | 7 | |a Transcription génétique |2 ram | |
| 650 | 4 | |a Cell Nucleus |x genetics |x Laboratory Manuals | |
| 650 | 4 | |a Cell Nucleus |x physiology |x Laboratory Manuals | |
| 650 | 4 | |a Cell nuclei |x Research |x Laboratory manuals | |
| 650 | 4 | |a Cytological Techniques |x Laboratory Manuals | |
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Datensatz im Suchindex
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|---|---|
| adam_text | Contents
Preface
..............................................................................................................
v
Contributors
.................................................................................................... xi
List of Color Plates
......................................................................................... xvii
Part I Physical and Mechanical Properties
1
Physical Properties of the Nucleus Studied
by
Micropipette
Aspiration
..................................................................... 3
Amy
С
Rowat
2
Mechanical Properties of
Interphase
Nuclei Probed
by Cellular Strain Application
................................................................ 13
Jan Lammerding
and Richard T. Lee
Part II Chromatin and Transcription
3
Gene Expression in Polytene Nuclei
........................................................ 29
Petra Björk
and
Lars Wieslander
4
Electron Microscope Visualization of
RNA
Transcription
and Processing in Saccharomyces cerevisiae
by Miller Chromatin Spreading
............................................................. 55
Yvonne
N.
Osheim, Sarah L. French, Martha L. Sikes,
and Ann L. Beyer
5
Combing Genomic
DNA
for Structural and Functional Studies
......... 71
Catherine Schurra and Aaron
Bensimon
6
Using Molecular Beacons to Study Dispersal of mRNPs
from the Gene Locus
................................................................................ 91
Patrick
Т. С
van
den Bogaard
and Sanjay Tyagi
vìii
Contents
7
Mapping
Cis-
and Trans- Chromatin Interaction Networks
Using Chromosome Conformation Capture (3C)
............................... 105
Adriana
Miele
and Job
Dekker
8
Recognition Imaging of Chromatin and Chromatin-Remodeling
Complexes in the Atomic Force Microscope
....................................... 123
Dennis Lohr, Hongda Wang, Ralph Bash, and Stuart M. Lindsay
9
Using Cells Encapsulated in
Agarose
Microbeads to Analyse
Nuclear Structure and Functions
......................................................... 139
Dean Jackson
Part III The Nuclear Envelope
10
Investigation of Nuclear Envelope Structure
and Passive Permeability
....................................................................... 161
Victor Shahin, Yvonne
Ludwig,
and Hans Oberleithner
11
Reconstitution
of Nuclear Import in Permeabffized Cells
.................. 181
Aurelia
Cassany and Larry Gerace
12
Nuclear Envelope Formation In Vitro: A Sea Urchin Egg
Cell-Free System
..................................................................................... 207
Richard D. Byrne, Vanessa Zhendre,
Banafshé
Larijani,
and Dominic L. Poccia
Part IV Modifications of Nuclear Proteins
13
Detection and Analysis of (O-linked
ß-jV-Acetylglucosamine)-
Modified Proteins
................................................................................... 227
Natasha E. Zachara
14
Detection of Sumoylated Proteins
......................................................... 255
Ok-Kyong Park-Sarge and Kevin D.
Sarge
15
Detection of the Nuclear Poly(ADP-ribose)-Metabolizing
Enzymes and Activities in Response to
DNA
Damage
........................ 267
Jean-Christophe
Amé,
Antoinette
Hakmé,
Delphine Quenet,
Elise
Fouquerel,
Françoise Dantzer,
and
Valérie
Schreiber
16
Purification and Analysis of Variant and Modified Histones
Using 2D PAGE
...................................................................................... 285
George R. Green and Due P. Do
Contents ix
17
Quantification
of Redox
Conditions in the Nucleuss
........................... 303
Young-Mi Go,
Jan Pohl,
and Dean P. Jones
Part V Protein Dynamics in the Nucleus
18
Fluorescence Correlation Spectroscopy to Assess the Mobility
of Nuclear Proteins
................................................................................. 321
Stefanie
Weidtkamp-Peters, Klaus Weisshart,
Lars Schmiedeberg,
and Peter
Hemmerich
19
Single Molecule Tracking for Studying Nucleocytoplasmic
Transport and Intranuclear Dynamics
................................................. 343
Jan Peter
Siebrasse
and
Ulrich Kubitscheck
20
Fluorescence Recovery After Photobleaching (FRAP)
to Study Nuclear Protein Dynamics in Living Cells
............................ 363
Martin E. van
Royen,
Pascal
Farla,
Karin
Α.
Mattern, Bart Geverts,
Jan
Trapman,
and Adriaan B.
Houtsmuller
Part
VI Imaging Methods
21 Nanosizing
by Spatially Modulated Illumination (SMI)
Microscopy and Applications to the Nucleus
...................................... 389
Udo
J. Birk,
David Baddeley, and
Christoph Cremer
22
Visualisation of
RNA
by Electron Microscopic
In Situ Hybridisation
............................................................................. 403
Jacques Rouquette,
Karl-Henning Kalland,
and
Stanislav Fakan
23
Electron
Spectroscopie
Imaging of the Nuclear Landscape
................ 415
Kashif Ahmed, Ren Li, and David P. Bazett-Jones
24
Cryoelectron Microscopy of Vitreous Sections: A Step Further
Towards the Native State
....................................................................... 425
Cedric Bouchet-Marquis and
Stanislav
Fakan
Index
................................................................................................................. 441
|
| adam_txt |
Contents
Preface
.
v
Contributors
. xi
List of Color Plates
. xvii
Part I Physical and Mechanical Properties
1
Physical Properties of the Nucleus Studied
by
Micropipette
Aspiration
. 3
Amy
С
Rowat
2
Mechanical Properties of
Interphase
Nuclei Probed
by Cellular Strain Application
. 13
Jan Lammerding
and Richard T. Lee
Part II Chromatin and Transcription
3
Gene Expression in Polytene Nuclei
. 29
Petra Björk
and
Lars Wieslander
4
Electron Microscope Visualization of
RNA
Transcription
and Processing in Saccharomyces cerevisiae
by Miller Chromatin Spreading
. 55
Yvonne
N.
Osheim, Sarah L. French, Martha L. Sikes,
and Ann L. Beyer
5
Combing Genomic
DNA
for Structural and Functional Studies
. 71
Catherine Schurra and Aaron
Bensimon
6
Using Molecular Beacons to Study Dispersal of mRNPs
from the Gene Locus
. 91
Patrick
Т. С
van
den Bogaard
and Sanjay Tyagi
vìii
Contents
7
Mapping
Cis-
and Trans- Chromatin Interaction Networks
Using Chromosome Conformation Capture (3C)
. 105
Adriana
Miele
and Job
Dekker
8
Recognition Imaging of Chromatin and Chromatin-Remodeling
Complexes in the Atomic Force Microscope
. 123
Dennis Lohr, Hongda Wang, Ralph Bash, and Stuart M. Lindsay
9
Using Cells Encapsulated in
Agarose
Microbeads to Analyse
Nuclear Structure and Functions
. 139
Dean Jackson
Part III The Nuclear Envelope
10
Investigation of Nuclear Envelope Structure
and Passive Permeability
. 161
Victor Shahin, Yvonne
Ludwig,
and Hans Oberleithner
11
Reconstitution
of Nuclear Import in Permeabffized Cells
. 181
Aurelia
Cassany and Larry Gerace
12
Nuclear Envelope Formation In Vitro: A Sea Urchin Egg
Cell-Free System
. 207
Richard D. Byrne, Vanessa Zhendre,
Banafshé
Larijani,
and Dominic L. Poccia
Part IV Modifications of Nuclear Proteins
13
Detection and Analysis of (O-linked
ß-jV-Acetylglucosamine)-
Modified Proteins
. 227
Natasha E. Zachara
14
Detection of Sumoylated Proteins
. 255
Ok-Kyong Park-Sarge and Kevin D.
Sarge
15
Detection of the Nuclear Poly(ADP-ribose)-Metabolizing
Enzymes and Activities in Response to
DNA
Damage
. 267
Jean-Christophe
Amé,
Antoinette
Hakmé,
Delphine Quenet,
Elise
Fouquerel,
Françoise Dantzer,
and
Valérie
Schreiber
16
Purification and Analysis of Variant and Modified Histones
Using 2D PAGE
. 285
George R. Green and Due P. Do
Contents ix
17
Quantification
of Redox
Conditions in the Nucleuss
. 303
Young-Mi Go,
Jan Pohl,
and Dean P. Jones
Part V Protein Dynamics in the Nucleus
18
Fluorescence Correlation Spectroscopy to Assess the Mobility
of Nuclear Proteins
. 321
Stefanie
Weidtkamp-Peters, Klaus Weisshart,
Lars Schmiedeberg,
and Peter
Hemmerich
19
Single Molecule Tracking for Studying Nucleocytoplasmic
Transport and Intranuclear Dynamics
. 343
Jan Peter
Siebrasse
and
Ulrich Kubitscheck
20
Fluorescence Recovery After Photobleaching (FRAP)
to Study Nuclear Protein Dynamics in Living Cells
. 363
Martin E. van
Royen,
Pascal
Farla,
Karin
Α.
Mattern, Bart Geverts,
Jan
Trapman,
and Adriaan B.
Houtsmuller
Part
VI Imaging Methods
21 Nanosizing
by Spatially Modulated Illumination (SMI)
Microscopy and Applications to the Nucleus
. 389
Udo
J. Birk,
David Baddeley, and
Christoph Cremer
22
Visualisation of
RNA
by Electron Microscopic
In Situ Hybridisation
. 403
Jacques Rouquette,
Karl-Henning Kalland,
and
Stanislav Fakan
23
Electron
Spectroscopie
Imaging of the Nuclear Landscape
. 415
Kashif Ahmed, Ren Li, and David P. Bazett-Jones
24
Cryoelectron Microscopy of Vitreous Sections: A Step Further
Towards the Native State
. 425
Cedric Bouchet-Marquis and
Stanislav
Fakan
Index
. 441 |
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| id | DE-604.BV023353058 |
| illustrated | Illustrated |
| index_date | 2024-07-02T21:05:54Z |
| indexdate | 2024-07-09T21:16:38Z |
| institution | BVB |
| isbn | 9781603274609 9781603274616 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-016536639 |
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| physical | XVIII, 444 S. Ill., graph. Darst. |
| publishDate | 2008 |
| publishDateSearch | 2008 |
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| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spelling | The nucleus 2 Chromatin, transcription, envelope, proteins, dynamics, and imaging Ronald Hancock, ed. Physical properties and imaging methods Totowa, NJ Humana Press 2008 XVIII, 444 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 464 Methods in molecular biology ... Noyau cellulaire ram Transcription génétique ram Cell Nucleus genetics Laboratory Manuals Cell Nucleus physiology Laboratory Manuals Cell nuclei Research Laboratory manuals Cytological Techniques Laboratory Manuals Zellkern (DE-588)4067544-0 gnd rswk-swf Kernhülle (DE-588)4309987-7 gnd rswk-swf Coiled Body (DE-588)4416414-2 gnd rswk-swf Nucleolus (DE-588)4212008-1 gnd rswk-swf Kernproteine (DE-588)4389511-6 gnd rswk-swf (DE-588)1071861417 Konferenzschrift 2007 Québec gnd-content Zellkern (DE-588)4067544-0 s DE-604 Kernproteine (DE-588)4389511-6 s Kernhülle (DE-588)4309987-7 s Nucleolus (DE-588)4212008-1 s Coiled Body (DE-588)4416414-2 s Hancock, Ronald Sonstige oth (DE-604)BV023353030 2 Methods in molecular biology 464 (DE-604)BV035362695 464 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016536639&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | The nucleus Methods in molecular biology Noyau cellulaire ram Transcription génétique ram Cell Nucleus genetics Laboratory Manuals Cell Nucleus physiology Laboratory Manuals Cell nuclei Research Laboratory manuals Cytological Techniques Laboratory Manuals Zellkern (DE-588)4067544-0 gnd Kernhülle (DE-588)4309987-7 gnd Coiled Body (DE-588)4416414-2 gnd Nucleolus (DE-588)4212008-1 gnd Kernproteine (DE-588)4389511-6 gnd |
| subject_GND | (DE-588)4067544-0 (DE-588)4309987-7 (DE-588)4416414-2 (DE-588)4212008-1 (DE-588)4389511-6 (DE-588)1071861417 |
| title | The nucleus |
| title_alt | Physical properties and imaging methods |
| title_auth | The nucleus |
| title_exact_search | The nucleus |
| title_exact_search_txtP | The nucleus |
| title_full | The nucleus 2 Chromatin, transcription, envelope, proteins, dynamics, and imaging Ronald Hancock, ed. |
| title_fullStr | The nucleus 2 Chromatin, transcription, envelope, proteins, dynamics, and imaging Ronald Hancock, ed. |
| title_full_unstemmed | The nucleus 2 Chromatin, transcription, envelope, proteins, dynamics, and imaging Ronald Hancock, ed. |
| title_short | The nucleus |
| title_sort | the nucleus chromatin transcription envelope proteins dynamics and imaging |
| topic | Noyau cellulaire ram Transcription génétique ram Cell Nucleus genetics Laboratory Manuals Cell Nucleus physiology Laboratory Manuals Cell nuclei Research Laboratory manuals Cytological Techniques Laboratory Manuals Zellkern (DE-588)4067544-0 gnd Kernhülle (DE-588)4309987-7 gnd Coiled Body (DE-588)4416414-2 gnd Nucleolus (DE-588)4212008-1 gnd Kernproteine (DE-588)4389511-6 gnd |
| topic_facet | Noyau cellulaire Transcription génétique Cell Nucleus genetics Laboratory Manuals Cell Nucleus physiology Laboratory Manuals Cell nuclei Research Laboratory manuals Cytological Techniques Laboratory Manuals Zellkern Kernhülle Coiled Body Nucleolus Kernproteine Konferenzschrift 2007 Québec |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016536639&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV023353030 (DE-604)BV035362695 |
| work_keys_str_mv | AT hancockronald thenucleus2 AT hancockronald physicalpropertiesandimagingmethods |