The nucleus: 1 Nuclei and subnuclear components
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2008
|
| Schriftenreihe: | Methods in molecular biology
463 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XVIII, 323 S. Ill., graph. Darst. |
| ISBN: | 9781588299772 9781597454063 |
Internformat
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Datensatz im Suchindex
| _version_ | 1804137711817195520 |
|---|---|
| adam_text | Contents
Preface v
Contributors xi
List of Color Plates xvii
Part I The Intranuclear Environment
1 The Intranuclear Environment 3
Santiago Schnell and Ronald Hancock
Part II Isolation Of Nuclei
2 Purification of Nuclei and Preparation of Nuclear
Envelopes from Skeletal Muscle 23
Gavin S. Wilkie and Eric C. Schirmer
3 Isolation of Highly Purified Yeast Nuclei for Nuclease
Mapping of Chromatin Structure 43
Joseph C. Reese, Hesheng Zhang, and Zhengjian Zhang
4 Working with Oocyte Nuclei: Cytological
Preparations of Active Chromatin and Nuclear
Bodies from Amphibian Germinal Vesicles 55
Garry T. Morgan
5 Preparation of Arabidopsis Nuclei and Nucleoli 67
Peter McKeown, Alison F. Pendle, and Peter J. Shaw
6 High-Yield Isolation and Subcellular Proteomic
Characterization of Nuclear and Subnuclear
Structures from Trypanosomes 77
Jeffrey A. DeGrasse, Brian T. Chait, Mark C. Field, and Michael P. Rout
vii
viii Contents
7 Methods for Studying the Nuclei and Chromosomes
ofDinoflagellates 93
Marie-Odile Soyer-Gobillard
Part III The Nucleolus
8 Isolation of Nucleoli from Ehrlich Ascites Tumor Cells
and Dynamics of Nascent RNA within Isolated Nucleoli Ill
Marc Thiry and Dominique Ploton
9 Time-lapse Microscopy and Fluorescence Resonance
Energy Transfer to Analyze the Dynamics
and Interactions of Nucleolar Proteins in Living Cells 123
Emilie Louvet, Marc Tramier, Nicole Angelier,
and Daniele Hernandez-Verdun
10 Three-Dimensional Reconstruction of Nucleolar
Components by Electron Microscope Tomography 137
Pavel Tchelidze, Herve Kaplan, Adrien Beorchia,
Marie-Frangoise O Donohue, Helene Bobichon,
Nathalie Lalun, Laurence Wortham, and Dominique Ploton
Part IV Intranuclear Structures
11 The Perinucleolar Compartment (PNC): Detection
by Immunohistochemistry 161
Alicja Slusarczyk and Sui Huang
12 Isolation of the Constitutive Heterochromatin from
Mouse Liver Nuclei 169
Olga V. Zatsepina, Oxana O. Zharskaya,
and Andrei N. Prusov
13 Isolation of Pathology-Associated
Intranuclear Inclusions 181
Christine Iwahashi and Paul J. Hagerman
14 The Nuclear Ubiquitin-Proteasome System:
Visualization of Proteasomes, Protein Aggregates,
and Proteolysis in the Cell Nucleus 191
Anna von Mikecz, Min Chen, Thomas Rockel, and Andrea Scharf
Contents ix
Part V Interphase Chromosomes
15 Multicolor 3D Fluorescence In Situ Hybridization
for Imaging Interphase Chromosomes 205
Marion Cremer, Florian Grasser, Christian Lanctot, Stefan Mtiller,
Michaela Neusser, Roman Zinner, Irina Solovei, and Thomas Cremer
16 Fluorescent Transgenes to Study Interphase Chromosomes
in Living Plants 241
Antonius J. M. Matzke, Bruno Huettel, Johannes van der Winden,
and Marjori Matzke
17 Analysis of Telomeres and Telomerase 267
Jiri Fajkus, Martina Dvorackova, and Eva Sykorova
18 Combined Immunofluorescence, RNA Fluorescent
In Situ Hybridization, and DNA Fluorescent
In Situ Hybridization to Study Chromatin Changes,
Transcriptional Activity, Nuclear Organization,
and X-Chromosome Inactivation 297
Julie Chaumeil, Sandrine Augui, Jennifer C. Chow,
and Edith Heard
19 Analysis of the Mobility of DNA Double-Strand
Break-Containing Chromosome Domains
in Living Mammalian Cells 309
Przemek M. Krawczyk, Jan Stap, Ron A. Hoebe, Carel H. van Oven,
Roland Kanaar, and Jacob A. Aten
Index 321
|
| adam_txt |
Contents
Preface v
Contributors xi
List of Color Plates xvii
Part I The Intranuclear Environment
1 The Intranuclear Environment 3
Santiago Schnell and Ronald Hancock
Part II Isolation Of Nuclei
2 Purification of Nuclei and Preparation of Nuclear
Envelopes from Skeletal Muscle 23
Gavin S. Wilkie and Eric C. Schirmer
3 Isolation of Highly Purified Yeast Nuclei for Nuclease
Mapping of Chromatin Structure 43
Joseph C. Reese, Hesheng Zhang, and Zhengjian Zhang
4 Working with Oocyte Nuclei: Cytological
Preparations of Active Chromatin and Nuclear
Bodies from Amphibian Germinal Vesicles 55
Garry T. Morgan
5 Preparation of Arabidopsis Nuclei and Nucleoli 67
Peter McKeown, Alison F. Pendle, and Peter J. Shaw
6 High-Yield Isolation and Subcellular Proteomic
Characterization of Nuclear and Subnuclear
Structures from Trypanosomes 77
Jeffrey A. DeGrasse, Brian T. Chait, Mark C. Field, and Michael P. Rout
vii
viii Contents
7 Methods for Studying the Nuclei and Chromosomes
ofDinoflagellates 93
Marie-Odile Soyer-Gobillard
Part III The Nucleolus
8 Isolation of Nucleoli from Ehrlich Ascites Tumor Cells
and Dynamics of Nascent RNA within Isolated Nucleoli Ill
Marc Thiry and Dominique Ploton
9 Time-lapse Microscopy and Fluorescence Resonance
Energy Transfer to Analyze the Dynamics
and Interactions of Nucleolar Proteins in Living Cells 123
Emilie Louvet, Marc Tramier, Nicole Angelier,
and Daniele Hernandez-Verdun
10 Three-Dimensional Reconstruction of Nucleolar
Components by Electron Microscope Tomography 137
Pavel Tchelidze, Herve Kaplan, Adrien Beorchia,
Marie-Frangoise O'Donohue, Helene Bobichon,
Nathalie Lalun, Laurence Wortham, and Dominique Ploton
Part IV Intranuclear Structures
11 The Perinucleolar Compartment (PNC): Detection
by Immunohistochemistry 161
Alicja Slusarczyk and Sui Huang
12 Isolation of the Constitutive Heterochromatin from
Mouse Liver Nuclei 169
Olga V. Zatsepina, Oxana O. Zharskaya,
and Andrei N. Prusov
13 Isolation of Pathology-Associated
Intranuclear Inclusions 181
Christine Iwahashi and Paul J. Hagerman
14 The Nuclear Ubiquitin-Proteasome System:
Visualization of Proteasomes, Protein Aggregates,
and Proteolysis in the Cell Nucleus 191
Anna von Mikecz, Min Chen, Thomas Rockel, and Andrea Scharf
Contents ix
Part V Interphase Chromosomes
15 Multicolor 3D Fluorescence In Situ Hybridization
for Imaging Interphase Chromosomes 205
Marion Cremer, Florian Grasser, Christian Lanctot, Stefan Mtiller,
Michaela Neusser, Roman Zinner, Irina Solovei, and Thomas Cremer
16 Fluorescent Transgenes to Study Interphase Chromosomes
in Living Plants 241
Antonius J. M. Matzke, Bruno Huettel, Johannes van der Winden,
and Marjori Matzke
17 Analysis of Telomeres and Telomerase 267
Jiri Fajkus, Martina Dvorackova, and Eva Sykorova
18 Combined Immunofluorescence, RNA Fluorescent
In Situ Hybridization, and DNA Fluorescent
In Situ Hybridization to Study Chromatin Changes,
Transcriptional Activity, Nuclear Organization,
and X-Chromosome Inactivation 297
Julie Chaumeil, Sandrine Augui, Jennifer C. Chow,
and Edith Heard
19 Analysis of the Mobility of DNA Double-Strand
Break-Containing Chromosome Domains
in Living Mammalian Cells 309
Przemek M. Krawczyk, Jan Stap, Ron A. Hoebe, Carel H. van Oven,
Roland Kanaar, and Jacob A. Aten
Index 321 |
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| language | English |
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| physical | XVIII, 323 S. Ill., graph. Darst. |
| publishDate | 2008 |
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| publisher | Humana Press |
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| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spelling | The nucleus 1 Nuclei and subnuclear components Ronald Hancock, ed. Totowa, NJ Humana Press 2008 XVIII, 323 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 463 Methods in molecular biology ... Noyau cellulaire ram Transcription génétique ram Zellkern (DE-588)4067544-0 gnd rswk-swf Coiled Body (DE-588)4416414-2 gnd rswk-swf Nucleolus (DE-588)4212008-1 gnd rswk-swf (DE-588)1071861417 Konferenzschrift 2007 Québec gnd-content Coiled Body (DE-588)4416414-2 s DE-604 Zellkern (DE-588)4067544-0 s Nucleolus (DE-588)4212008-1 s Hancock, Ronald Sonstige oth (DE-604)BV023353030 1 Methods in molecular biology 463 (DE-604)BV035362695 463 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016536626&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | The nucleus Methods in molecular biology Noyau cellulaire ram Transcription génétique ram Zellkern (DE-588)4067544-0 gnd Coiled Body (DE-588)4416414-2 gnd Nucleolus (DE-588)4212008-1 gnd |
| subject_GND | (DE-588)4067544-0 (DE-588)4416414-2 (DE-588)4212008-1 (DE-588)1071861417 |
| title | The nucleus |
| title_auth | The nucleus |
| title_exact_search | The nucleus |
| title_exact_search_txtP | The nucleus |
| title_full | The nucleus 1 Nuclei and subnuclear components Ronald Hancock, ed. |
| title_fullStr | The nucleus 1 Nuclei and subnuclear components Ronald Hancock, ed. |
| title_full_unstemmed | The nucleus 1 Nuclei and subnuclear components Ronald Hancock, ed. |
| title_short | The nucleus |
| title_sort | the nucleus nuclei and subnuclear components |
| topic | Noyau cellulaire ram Transcription génétique ram Zellkern (DE-588)4067544-0 gnd Coiled Body (DE-588)4416414-2 gnd Nucleolus (DE-588)4212008-1 gnd |
| topic_facet | Noyau cellulaire Transcription génétique Zellkern Coiled Body Nucleolus Konferenzschrift 2007 Québec |
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