Bioanalytical techniques:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Oxford
Alpha Science Internat.
2008
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 458 S. Ill., graph. Darst. |
| ISBN: | 9781842654255 |
Internformat
MARC
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| 100 | 1 | |a Srivastava, Manish |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Bioanalytical techniques |c M. L. Srivastava |
| 264 | 1 | |a Oxford |b Alpha Science Internat. |c 2008 | |
| 300 | |a 458 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 650 | 4 | |a Analytical biochemistry | |
| 650 | 4 | |a Analytical biotechnology | |
| 650 | 4 | |a Chemistry Techniques, Analytical |x methods | |
| 650 | 4 | |a DNA, Recombinant | |
| 650 | 4 | |a Genetic Engineering | |
| 650 | 4 | |a Molecular Probe Techniques | |
| 650 | 4 | |a Sequence Analysis, DNA | |
| 650 | 0 | 7 | |a Biochemische Analyse |0 (DE-588)4255721-5 |2 gnd |9 rswk-swf |
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| 689 | 1 | 1 | |a Analytische Methode |0 (DE-588)4142352-5 |D s |
| 689 | 1 | |C b |5 DE-604 | |
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| 999 | |a oai:aleph.bib-bvb.de:BVB01-016530808 | ||
Datensatz im Suchindex
| _version_ | 1804137702966165504 |
|---|---|
| adam_text | Contents
Preface
vii
1.
Introduction
.........................................................................................................................................1-27
Introduction, Oparin s
hypothesis,
Miller s Experiments, Genetic Material,
Griffith s effect, Bacterial Recombination,
DNA-
Replication, Watson-Crick Model
of
DNA,
Forms of
DNA, Z
DNA
(left banded
DNA),
Double Helical Structure of
DNA, Chargaff s
Rule, E.Coli
:
The Early Workhorse, Nucleus, Chromosomes,
Replication of
DNA,
Nucleic Acids, Structure of
DNA, DNA
as Genetic Material,
RNA
,
Type of RNA/ Function of
RNA, Gene,
Genetic Code, Features of Genetic
Code, Wobble Hypothesis, Concepts of Gene
Section
-1 :
GENERAL PRINCIPLES AND TECHNIQUES
2.
Microscopy
........................................................................................................................................29-47
Introduction, Invention of First Microscope, Modern Microscopic Techniques,
Light Microscopy, Construction and Working of a Compound Microscope,
Compound (Bright Field) Microscope, Dark Field Microscopy, Phase Contrast
Microscopy, Fluorescence Microscopy, Electron Microscopy, Transmission
Electron Microscopy or
ТЕМ,
Scanning Electron Microscope, Limitations of
Electron Microscopy, Ultraviolet Microscopy
3.
Aqueous Solutions
...........................................................................................................................49-56
Introduction, Water, Acids, Bases and Buffers, Zwitterionic Compounds,
Physiological Solutions
4.
Spectroscopy
.....................................................................................................................................57-66
Introduction, Electromagnetic Spectroscopy, Absorption Spectroscopy, Beer-
Lambert Law, Atomic Spectroscopy, Electron Spectroscopy, Laser Spectroscopy,
Molecular Spectroscopy, Ultraviolet and Visible Absorption Spectroscopy,
Nuclear and Magnetic Spectroscopy,
Х
-Ray & Gamma Ray Spectroscopy, Mass
Spectroscopy
5.
Centrifugation
...................................................................................................................................67-73
Introduction, Theory of Centrifugation, Types of Centrifugation, Density Gradient
Centrifugation, Types of Centrifuges
6.
Chromatography
...............................................................................................................................75-90
Introduction, Principles, Distribution of Analyte Phases, Equipment and Basic
Operation, Chromatogram, Adsorption Chromatography, Partition
Chromatography, Paper Chromatography,
Hydrophobie
Interaction
Chromatography, Ion Chromatography, Affinity Chromatography, Gas
Chromatography, HPLC
χ
Contents
7. Electrophoresis...............................................................................................................................91-100
Introduction, Types of Electrophoresis, Agar/Agarose Gel Electrophoresis,
Gradient Gel Electrophoresis, Capillary Electrophoresis
(CE),
DNA
Fingerprinting, Human Genome Project
8.
Radioisotopic Techniques
..........................................................................................................101-112
Introduction,
Radioisotopes
and Radioactivity, Types of Radioactivity,
Isotopie
Labeling, Detection and Measurement Radioactivity, Scintillation Counting
Section
- 2 :
ENZYME AND PROTEINS
9.
Protein Purification
......................................................................................................................113-121
Introduction, Protein Mixtures, General Features, Electrophoresis, Protein
Quantitation Assays, Protein Purification, Criteria for Purity, Purified Proteins,
Importance of Protein Purification
10.
Structure of Proteins
..................................................................................................................123-126
Introduction, Crystallization, NMR Spectroscopy, X-ray Diffraction
11.
Peptide
Sequencing
...................................................................................................................127-134
Introduction, Separation, Purification and Characterization of
Peptide
Chains,
Mass Spectrometers, Recording of Sample Ions, Electrospray, Specific Cleavage
Reaction
12.
Mapping and Synthesis
............................................................................................................135-143
Introduction, Epitopes Mapping, Principle of Display Technique, Epitope
Mapping Technology, Solid Supports, Protection, Chain Elongation, Cleavage
13.
Enzyme Kinetics
........................................................................................................................145-176
Introduction, Characteristic of Enzymes, Enzyme Specificity, Classification of
Enzymes, The Kinetics of Enzyme Reaction, Influence of Substrate Concentration,
Inhibitors, The Proteolytic Enzymes, The Endopeptidases, The Oxidative Enzymes,
Splitting, Transferring and Isomerizing Enzymes, Enzymatic Abnormalities,
Immobilized Enzymes
14.
Immobilized Enzymes
..............................................................................................................177-182
Introduction, Preparation Techniques, Adsorption, Occlusion, Cross-linking,
Covalent Binding, Immobilization Method, Enzyme Reactor, Kinetic Properties
Section-
3 :
IMMUNOLOGICAL ANALYSIS
15.
Antigen and Antibody Techniques
........................................................................................183-199
Introduction, Measurement of Antibody Affinity, Types of Antigen-Antibody
Reactions, Precipitation Reactions, Monoclonal Antibodies, Purifying Antibodies,
Monoclonal Therapy
Section-
4: DNA
TECHNIQUES
16.
Nucleic Acid
................................................................................................................................201-212
Introduction, Biological Importance, Nucleoproteins, Pyrimidine Bases,
Purine
Bases, Nucleotides, Biological Function of Nucleic Acids
Contents xi
17. DNA.......................................................................................................................................213-225
Introduction,
Phosphoric Acid
, Pentose
Sugar,
Nitrogeneous Bases, DNA,
Chargaffs Base Ratio, Double
Helical Structure of
DNA,
Z-DNA and
B-DNA,
Heteroduplex
DNA, Ribonucleic
Acid
18.
RNA .......................................................................................................................................
227-237
Introduction, Ribosomal
RNA,
Messenger
RNA,
Transfer
RNA,
Three-
dimensional Structure,
DNA
Replication, Stahl s Experiment, Untwisting Proteins,
RNA
Directed
DNA
Synthesis, Eukaryotic
DNA Polymerase,
Genetic Material
19. DNA
Modifying Enzymes
........................................................................................................239-268
Introduction, Genomic
DNA,
Getting
DNA, DNA
Isolation, Purification of
DNA,
Precipitation of
DNA, DNA
Analysis,
RNA
Purity, Restriction Enzymes,
DNA
Fractionation, Bacteriophage, Cosmids
20.
Immunochemical Assays
.........................................................................................................269-283
Introduction, Radioimmunoassay, Enzyme Linked Antibody Capture, ELISpot
A, Fluoroscent Antibody, Western Immunoblotting, Electrophoresis of Protein,
Transblotting Protein, Immunoprecipitation, Immunofluorescence, Fluorescence
Activated Cell, Immunoelectron Microscopy, Immunohistochemistry, Complement
System
21.
Immunology
................................................................................................................................285-307
Introduction, Type of Immunity, Characters of Antigen, Immunoglobulin, Immune
System, Cells of Immune System, Properties of Immunoglobuling, Migration
Inhibitory Factor (MIF), Immune Electrophoresis, Manipulation of Immune System,
The Arthus Reaction
22.
PCR Techniques
.........................................................................................................................309-320
Introduction, Template
DNA,
Design of PCR, Thermostable
DNA,
Template
Denaturization, Annealing Temperature, Modifications of PCR, RAPD,
Application of PCR
Section-5: CELL TECHNOLOGY
23.
Eukaryotic Cells
..........................................................................................................................321-334
Introduction, Laboratory Design, Microbiological Safety Cabinets, Safety Aspects
of Cell Culture, General Handling Techniques, Cell Culture, Cell Environment,
Prokaryotic Cell
24.
Fermentation Process
................................................................................................................335-345
Introduction, Artificial Fermentation, Design of Industrial Fermentation, Cellular
Catalysts, Bioreactors, Modes of Bioreactor Operation, Fermentation Kinetics,
Downstream Processing of Fermented Products
Section
- 6 :
GENETIC ENGINEERING
25.
Genetic Engineering
..................................................................................................................347-352
Introduction, Isolation of
DNA, Gene
Theory,
Recombinant
DNA
xii Contents
26. Tools
of Genetic
Engineering..................................................................................................353-370
Introduction,
Morgan Experiment,
Restriction Enzymes, Endonucleases,
Isolation
of
DNA,
Cloning Vectors
27.
Techniques of Genetic Engineering
.......................................................................................371-401
Introduction, Cloning, Gene Cloning, Dolly, Hybridization, Immunological
Detection, Radioactive Labeling,
DNA
Sequencing, Model for PEG Action,
DNA
Delivery,
Microinjection
28.
Genetic Engineering for Human Health
...............................................................................403-427
Introduction, HIV/AIDS, Hepatitis, Leprosy, Polio, Diabetes, Mental Health,
Malnutrition,
Recombinant
DNA, Gene
Therapy, Genetic Counselling, Gene
Synthesis, Human Genetics, Sex-Differentiation, Sex-Linked Inheritance
Glossary
.......................................................................................................................................429-452
Index
................................................................................................................................................453-458
|
| adam_txt |
Contents
Preface
vii
1.
Introduction
.1-27
Introduction, Oparin's
hypothesis,
Miller's Experiments, Genetic Material,
Griffith's effect, Bacterial Recombination,
DNA-
Replication, Watson-Crick Model
of
DNA,
Forms of
DNA, Z
DNA
(left banded
DNA),
Double Helical Structure of
DNA, Chargaff's
Rule, E.Coli
:
The Early Workhorse, Nucleus, Chromosomes,
Replication of
DNA,
Nucleic Acids, Structure of
DNA, DNA
as Genetic Material,
RNA
,
Type of RNA/ Function of
RNA, Gene,
Genetic Code, Features of Genetic
Code, Wobble Hypothesis, Concepts of Gene
Section
-1 :
GENERAL PRINCIPLES AND TECHNIQUES
2.
Microscopy
.29-47
Introduction, Invention of First Microscope, Modern Microscopic Techniques,
Light Microscopy, Construction and Working of a Compound Microscope,
Compound (Bright Field) Microscope, Dark Field Microscopy, Phase Contrast
Microscopy, Fluorescence Microscopy, Electron Microscopy, Transmission
Electron Microscopy or
ТЕМ,
Scanning Electron Microscope, Limitations of
Electron Microscopy, Ultraviolet Microscopy
3.
Aqueous Solutions
.49-56
Introduction, Water, Acids, Bases and Buffers, Zwitterionic Compounds,
Physiological Solutions
4.
Spectroscopy
.57-66
Introduction, Electromagnetic Spectroscopy, Absorption Spectroscopy, Beer-
Lambert Law, Atomic Spectroscopy, Electron Spectroscopy, Laser Spectroscopy,
Molecular Spectroscopy, Ultraviolet and Visible Absorption Spectroscopy,
Nuclear and Magnetic Spectroscopy,
Х
-Ray & Gamma Ray Spectroscopy, Mass
Spectroscopy
5.
Centrifugation
.67-73
Introduction, Theory of Centrifugation, Types of Centrifugation, Density Gradient
Centrifugation, Types of Centrifuges
6.
Chromatography
.75-90
Introduction, Principles, Distribution of Analyte Phases, Equipment and Basic
Operation, Chromatogram, Adsorption Chromatography, Partition
Chromatography, Paper Chromatography,
Hydrophobie
Interaction
Chromatography, Ion Chromatography, Affinity Chromatography, Gas
Chromatography, HPLC
χ
Contents
7. Electrophoresis.91-100
Introduction, Types of Electrophoresis, Agar/Agarose Gel Electrophoresis,
Gradient Gel Electrophoresis, Capillary Electrophoresis
(CE),
DNA
Fingerprinting, Human Genome Project
8.
Radioisotopic Techniques
.101-112
Introduction,
Radioisotopes
and Radioactivity, Types of Radioactivity,
Isotopie
Labeling, Detection and Measurement Radioactivity, Scintillation Counting
Section
- 2 :
ENZYME AND PROTEINS
9.
Protein Purification
.113-121
Introduction, Protein Mixtures, General Features, Electrophoresis, Protein
Quantitation Assays, Protein Purification, Criteria for Purity, Purified Proteins,
Importance of Protein Purification
10.
Structure of Proteins
.123-126
Introduction, Crystallization, NMR Spectroscopy, X-ray Diffraction
11.
Peptide
Sequencing
.127-134
Introduction, Separation, Purification and Characterization of
Peptide
Chains,
Mass Spectrometers, Recording of Sample Ions, Electrospray, Specific Cleavage
Reaction
12.
Mapping and Synthesis
.135-143
Introduction, Epitopes Mapping, Principle of Display Technique, Epitope
Mapping Technology, Solid Supports, Protection, Chain Elongation, Cleavage
13.
Enzyme Kinetics
.145-176
Introduction, Characteristic of Enzymes, Enzyme Specificity, Classification of
Enzymes, The Kinetics of Enzyme Reaction, Influence of Substrate Concentration,
Inhibitors, The Proteolytic Enzymes, The Endopeptidases, The Oxidative Enzymes,
Splitting, Transferring and Isomerizing Enzymes, Enzymatic Abnormalities,
Immobilized Enzymes
14.
Immobilized Enzymes
.177-182
Introduction, Preparation Techniques, Adsorption, Occlusion, Cross-linking,
Covalent Binding, Immobilization Method, Enzyme Reactor, Kinetic Properties
Section-
3 :
IMMUNOLOGICAL ANALYSIS
15.
Antigen and Antibody Techniques
.183-199
Introduction, Measurement of Antibody Affinity, Types of Antigen-Antibody
Reactions, Precipitation Reactions, Monoclonal Antibodies, Purifying Antibodies,
Monoclonal Therapy
Section-
4: DNA
TECHNIQUES
16.
Nucleic Acid
.201-212
Introduction, Biological Importance, Nucleoproteins, Pyrimidine Bases,
Purine
Bases, Nucleotides, Biological Function of Nucleic Acids
Contents xi
17. DNA.213-225
Introduction,
Phosphoric Acid
, Pentose
Sugar,
Nitrogeneous Bases, DNA,
Chargaffs Base Ratio, Double
Helical Structure of
DNA,
Z-DNA and
B-DNA,
Heteroduplex
DNA, Ribonucleic
Acid
18.
RNA .
227-237
Introduction, Ribosomal
RNA,
Messenger
RNA,
Transfer
RNA,
Three-
dimensional Structure,
DNA
Replication, Stahl's Experiment, Untwisting Proteins,
RNA
Directed
DNA
Synthesis, Eukaryotic
DNA Polymerase,
Genetic Material
19. DNA
Modifying Enzymes
.239-268
Introduction, Genomic
DNA,
Getting
DNA, DNA
Isolation, Purification of
DNA,
Precipitation of
DNA, DNA
Analysis,
RNA
Purity, Restriction Enzymes,
DNA
Fractionation, Bacteriophage, Cosmids
20.
Immunochemical Assays
.269-283
Introduction, Radioimmunoassay, Enzyme Linked Antibody Capture, ELISpot
A, Fluoroscent Antibody, Western Immunoblotting, Electrophoresis of Protein,
Transblotting Protein, Immunoprecipitation, Immunofluorescence, Fluorescence
Activated Cell, Immunoelectron Microscopy, Immunohistochemistry, Complement
System
21.
Immunology
.285-307
Introduction, Type of Immunity, Characters of Antigen, Immunoglobulin, Immune
System, Cells of Immune System, Properties of Immunoglobuling, Migration
Inhibitory Factor (MIF), Immune Electrophoresis, Manipulation of Immune System,
The Arthus Reaction
22.
PCR Techniques
.309-320
Introduction, Template
DNA,
Design of PCR, Thermostable
DNA,
Template
Denaturization, Annealing Temperature, Modifications of PCR, RAPD,
Application of PCR
Section-5: CELL TECHNOLOGY
23.
Eukaryotic Cells
.321-334
Introduction, Laboratory Design, Microbiological Safety Cabinets, Safety Aspects
of Cell Culture, General Handling Techniques, Cell Culture, Cell Environment,
Prokaryotic Cell
24.
Fermentation Process
.335-345
Introduction, Artificial Fermentation, Design of Industrial Fermentation, Cellular
Catalysts, Bioreactors, Modes of Bioreactor Operation, Fermentation Kinetics,
Downstream Processing of Fermented Products
Section
- 6 :
GENETIC ENGINEERING
25.
Genetic Engineering
.347-352
Introduction, Isolation of
DNA, Gene
Theory,
Recombinant
DNA
xii Contents
26. Tools
of Genetic
Engineering.353-370
Introduction,
Morgan Experiment,
Restriction Enzymes, Endonucleases,
Isolation
of
DNA,
Cloning Vectors
27.
Techniques of Genetic Engineering
.371-401
Introduction, Cloning, Gene Cloning, Dolly, Hybridization, Immunological
Detection, Radioactive Labeling,
DNA
Sequencing, Model for PEG Action,
DNA
Delivery,
Microinjection
28.
Genetic Engineering for Human Health
.403-427
Introduction, HIV/AIDS, Hepatitis, Leprosy, Polio, Diabetes, Mental Health,
Malnutrition,
Recombinant
DNA, Gene
Therapy, Genetic Counselling, Gene
Synthesis, Human Genetics, Sex-Differentiation, Sex-Linked Inheritance
Glossary
.429-452
Index
.453-458 |
| any_adam_object | 1 |
| any_adam_object_boolean | 1 |
| author | Srivastava, Manish |
| author_facet | Srivastava, Manish |
| author_role | aut |
| author_sort | Srivastava, Manish |
| author_variant | m s ms |
| building | Verbundindex |
| bvnumber | BV023347144 |
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| callnumber-label | QP519 |
| callnumber-raw | QP519.7 |
| callnumber-search | QP519.7 |
| callnumber-sort | QP 3519.7 |
| callnumber-subject | QP - Physiology |
| classification_rvk | WC 1200 WC 4150 |
| ctrlnum | (OCoLC)166361248 (DE-599)BVBBV023347144 |
| dewey-full | 572.36 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 572 - Biochemistry |
| dewey-raw | 572.36 |
| dewey-search | 572.36 |
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| discipline | Biologie |
| discipline_str_mv | Biologie |
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| id | DE-604.BV023347144 |
| illustrated | Illustrated |
| index_date | 2024-07-02T21:03:50Z |
| indexdate | 2024-07-09T21:16:30Z |
| institution | BVB |
| isbn | 9781842654255 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-016530808 |
| oclc_num | 166361248 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-29T DE-11 |
| owner_facet | DE-355 DE-BY-UBR DE-29T DE-11 |
| physical | 458 S. Ill., graph. Darst. |
| publishDate | 2008 |
| publishDateSearch | 2008 |
| publishDateSort | 2008 |
| publisher | Alpha Science Internat. |
| record_format | marc |
| spelling | Srivastava, Manish Verfasser aut Bioanalytical techniques M. L. Srivastava Oxford Alpha Science Internat. 2008 458 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Analytical biochemistry Analytical biotechnology Chemistry Techniques, Analytical methods DNA, Recombinant Genetic Engineering Molecular Probe Techniques Sequence Analysis, DNA Biochemische Analyse (DE-588)4255721-5 gnd rswk-swf Zelle (DE-588)4067537-3 gnd rswk-swf Analytische Methode (DE-588)4142352-5 gnd rswk-swf Biochemische Analyse (DE-588)4255721-5 s DE-604 Zelle (DE-588)4067537-3 s Analytische Methode (DE-588)4142352-5 s b DE-604 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016530808&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Srivastava, Manish Bioanalytical techniques Analytical biochemistry Analytical biotechnology Chemistry Techniques, Analytical methods DNA, Recombinant Genetic Engineering Molecular Probe Techniques Sequence Analysis, DNA Biochemische Analyse (DE-588)4255721-5 gnd Zelle (DE-588)4067537-3 gnd Analytische Methode (DE-588)4142352-5 gnd |
| subject_GND | (DE-588)4255721-5 (DE-588)4067537-3 (DE-588)4142352-5 |
| title | Bioanalytical techniques |
| title_auth | Bioanalytical techniques |
| title_exact_search | Bioanalytical techniques |
| title_exact_search_txtP | Bioanalytical techniques |
| title_full | Bioanalytical techniques M. L. Srivastava |
| title_fullStr | Bioanalytical techniques M. L. Srivastava |
| title_full_unstemmed | Bioanalytical techniques M. L. Srivastava |
| title_short | Bioanalytical techniques |
| title_sort | bioanalytical techniques |
| topic | Analytical biochemistry Analytical biotechnology Chemistry Techniques, Analytical methods DNA, Recombinant Genetic Engineering Molecular Probe Techniques Sequence Analysis, DNA Biochemische Analyse (DE-588)4255721-5 gnd Zelle (DE-588)4067537-3 gnd Analytische Methode (DE-588)4142352-5 gnd |
| topic_facet | Analytical biochemistry Analytical biotechnology Chemistry Techniques, Analytical methods DNA, Recombinant Genetic Engineering Molecular Probe Techniques Sequence Analysis, DNA Biochemische Analyse Zelle Analytische Methode |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016530808&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT srivastavamanish bioanalyticaltechniques |