Verfügbarkeit: Drosophila :: THWS Bibkatalog

Drosophila: methods and protocols

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Format:	Buch
Sprache:	English
Veröffentlicht:	Totowa, NJ Humana Press 2008
Schriftenreihe:	Methods in molecular biology 420
Schlagworte:	Drosophila Molecular biology / Research Molecular biology / Technique Animal models in research Drosophila melanogaster / Laboratory Manuals Molecular Biology / methods Models, Animal Drosophila melanogaster > Laboratory Manuals Models, Animal > Laboratory Manuals Molecular Biology > methods > Laboratory Manuals Molecular biology > Research Molecular biology > Technique Genetik Taufliege Molekularbiologie Methode Aufsatzsammlung
Online-Zugang:	Inhaltsverzeichnis
Beschreibung:	XI, 437 S. Ill., graph. Darst.
ISBN:	9781588298171 9781597455831

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Datensatz im Suchindex

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adam_text	Contents Preface ................................................................................................................ v Contributors ....................................................................................................... /χ 1 Drosophila melanogaster and the Development of Biology in the 20th Century Alfonso Martinez Arias ...............................................................................1 2 Getting Started: An Overview on Raising and Handling Drosophila Hugo Stocker and Peter Gallant ..............................................................27 3 FlyBase: A Database for the Drosophila Research Community Rachel Drysdale and the FlyBase Consortium ..........................................45 4 The Use of P-Element Transposons to Generate Transgenic Flies André Bachmann and Elisabeth Knust. .....................................................61 5 The GAL4 System: A Versatile System for the Expression of Genes David A. Elliott and Andrea H. Brand ......................................................79 6 P-Element Mutagenesis Thomas Hummel and Christian Klämbt ...................................................97 7 EMS Screens: From Mutagenesis to Screening and Mapping Christian Bökel.......................................................................................119 8 RNAi Screening in Cultured Drosophila Cells Sandra Steinbrink and Michael Boutros.................................................139 9 Methods for Homologous Recombination in Drosophila Keith Α. Maggert, Weif. Gong, and Kent G. Golic ................................155 10 Recombinases and Their Use in Gene Activation, Gene Inactivation, and Transgenesis Johannes Bischof and Konrad Basler......................................................175 11 Cuticle Preparation of Drosophila Embryos and Larvae Cyrille Alexandre ...................................................................................1 97 12 Immunolabefing of Embryos H.-Arno ƒ. Müller...................................................................................207 13 Imaging Cellular and Molecular Dynamics in Live Embryos Using Fluorescent Proteins Matthieu Cavey and Thomas Lecuit ........................................................279 14 Analysis of Neuromuscular junctions: Histology and In Vivo Imaging Andreas Schmid and Stephan ƒ. Sigrist..... ..............................................239 VII viii Contents 15 Immunolabelíng of Imaginai Discs Thomas Klein ..........................................................................................253 16 Imaging Drosophila Pupal Wing Morphogenesis Anne-Kathrin Classen, Benoit Aigouy, Angela Giangrande, and Suzanne Eaton ............................................................................265 17 Microscopic Analysis of the Adult Drosophila Retina Using Semithin Plastic Sections Konstantin Gaengel and Marek Młodzik................................................ 277 18 Fluorescent In Situ Hybridization Protocols in Drosophila Embryos and Tissues Eric Lécuyer, Neela Parthasarathy, and Henry M. Krause .......................289 19 Linear RNA Amplification for the Production of Microarray Hybridization Probes Ansgar Klebes and Thomas B. Kornberg .................................................303 20 An Overview of the Identification, Detection, and Functional Analysis of Drosophila MicroRNAs Nicholas S. Sokol. ...................................................................................319 21 Extraction and Immunoblotting of Proteins From Embryos Andreas Wodarz .....................................................................................335 22 Purification of Drosophila Protein Complexes for Mass Spectrometry Christoph Jüschke and Jürgen A. Knoblich .............................................347 23 Mass Production of Drosophila Embryos and Chromatographie Purification of Native Protein Complexes Natascha Kunért and Alexander Brehm .................................................359 24 Flow Cytometric Analysis of Drosophila Cells Aida Flor A. de la Cruz and Bruce A. Edgar ...........................................373 25 Drosophila Cell Lines as Model Systems and as an Experimental Tool Buzz Baum and Lucy Cherbas ................................................................391 Appendix: Phenotypic Markers in Drosophila Jennifer Childress and Georg Halder..................................................... 425 Index .................................................................................................................429
adam_txt	Contents Preface . v Contributors . /χ 1 Drosophila melanogaster and the Development of Biology in the 20th Century Alfonso Martinez Arias .1 2 Getting Started: An Overview on Raising and Handling Drosophila Hugo Stocker and Peter Gallant .27 3 FlyBase: A Database for the Drosophila Research Community Rachel Drysdale and the FlyBase Consortium .45 4 The Use of P-Element Transposons to Generate Transgenic Flies André Bachmann and Elisabeth Knust. .61 5 The GAL4 System: A Versatile System for the Expression of Genes David A. Elliott and Andrea H. Brand .79 6 P-Element Mutagenesis Thomas Hummel and Christian Klämbt .97 7 EMS Screens: From Mutagenesis to Screening and Mapping Christian Bökel.119 8 RNAi Screening in Cultured Drosophila Cells Sandra Steinbrink and Michael Boutros.139 9 Methods for Homologous Recombination in Drosophila Keith Α. Maggert, Weif. Gong, and Kent G. Golic .155 10 Recombinases and Their Use in Gene Activation, Gene Inactivation, and Transgenesis Johannes Bischof and Konrad Basler.175 11 Cuticle Preparation of Drosophila Embryos and Larvae Cyrille Alexandre .1 97 12 Immunolabefing of Embryos H.-Arno ƒ. Müller.207 13 Imaging Cellular and Molecular Dynamics in Live Embryos Using Fluorescent Proteins Matthieu Cavey and Thomas Lecuit .279 14 Analysis of Neuromuscular junctions: Histology and In Vivo Imaging Andreas Schmid and Stephan ƒ. Sigrist. .239 VII viii Contents 15 Immunolabelíng of Imaginai Discs Thomas Klein .253 16 Imaging Drosophila Pupal Wing Morphogenesis Anne-Kathrin Classen, Benoit Aigouy, Angela Giangrande, and Suzanne Eaton .265 17 Microscopic Analysis of the Adult Drosophila Retina Using Semithin Plastic Sections Konstantin Gaengel and Marek Młodzik. 277 18 Fluorescent In Situ Hybridization Protocols in Drosophila Embryos and Tissues Eric Lécuyer, Neela Parthasarathy, and Henry M. Krause .289 19 Linear RNA Amplification for the Production of Microarray Hybridization Probes Ansgar Klebes and Thomas B. Kornberg .303 20 An Overview of the Identification, Detection, and Functional Analysis of Drosophila MicroRNAs Nicholas S. Sokol. .319 21 Extraction and Immunoblotting of Proteins From Embryos Andreas Wodarz .335 22 Purification of Drosophila Protein Complexes for Mass Spectrometry Christoph Jüschke and Jürgen A. Knoblich .347 23 Mass Production of Drosophila Embryos and Chromatographie Purification of Native Protein Complexes Natascha Kunért and Alexander Brehm .359 24 Flow Cytometric Analysis of Drosophila Cells Aida Flor A. de la Cruz and Bruce A. Edgar .373 25 Drosophila Cell Lines as Model Systems and as an Experimental Tool Buzz Baum and Lucy Cherbas .391 Appendix: Phenotypic Markers in Drosophila Jennifer Childress and Georg Halder. 425 Index .429
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spelling	Drosophila methods and protocols ed. by Christian Dahmann Totowa, NJ Humana Press 2008 XI, 437 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 420 Drosophila Molecular biology / Research Molecular biology / Technique Animal models in research Drosophila melanogaster / Laboratory Manuals Molecular Biology / methods Models, Animal Drosophila melanogaster Laboratory Manuals Models, Animal Laboratory Manuals Molecular Biology methods Laboratory Manuals Molecular biology Research Molecular biology Technique Genetik (DE-588)4071711-2 gnd rswk-swf Taufliege (DE-588)4078168-9 gnd rswk-swf Molekularbiologie (DE-588)4039983-7 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Drosophila (DE-588)4150708-3 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Taufliege (DE-588)4078168-9 s Molekularbiologie (DE-588)4039983-7 s Methode (DE-588)4038971-6 s DE-604 Drosophila (DE-588)4150708-3 s DE-188 Genetik (DE-588)4071711-2 s Dahmann, Christian 1966- Sonstige (DE-588)1147022240 oth Methods in molecular biology 420 (DE-604)BV035362695 420 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016516442&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis
spellingShingle	Drosophila methods and protocols Methods in molecular biology Drosophila Molecular biology / Research Molecular biology / Technique Animal models in research Drosophila melanogaster / Laboratory Manuals Molecular Biology / methods Models, Animal Drosophila melanogaster Laboratory Manuals Models, Animal Laboratory Manuals Molecular Biology methods Laboratory Manuals Molecular biology Research Molecular biology Technique Genetik (DE-588)4071711-2 gnd Taufliege (DE-588)4078168-9 gnd Molekularbiologie (DE-588)4039983-7 gnd Methode (DE-588)4038971-6 gnd Drosophila (DE-588)4150708-3 gnd
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title	Drosophila methods and protocols
title_auth	Drosophila methods and protocols
title_exact_search	Drosophila methods and protocols
title_exact_search_txtP	Drosophila methods and protocols
title_full	Drosophila methods and protocols ed. by Christian Dahmann
title_fullStr	Drosophila methods and protocols ed. by Christian Dahmann
title_full_unstemmed	Drosophila methods and protocols ed. by Christian Dahmann
title_short	Drosophila
title_sort	drosophila methods and protocols
title_sub	methods and protocols
topic	Drosophila Molecular biology / Research Molecular biology / Technique Animal models in research Drosophila melanogaster / Laboratory Manuals Molecular Biology / methods Models, Animal Drosophila melanogaster Laboratory Manuals Models, Animal Laboratory Manuals Molecular Biology methods Laboratory Manuals Molecular biology Research Molecular biology Technique Genetik (DE-588)4071711-2 gnd Taufliege (DE-588)4078168-9 gnd Molekularbiologie (DE-588)4039983-7 gnd Methode (DE-588)4038971-6 gnd Drosophila (DE-588)4150708-3 gnd
topic_facet	Drosophila Molecular biology / Research Molecular biology / Technique Animal models in research Drosophila melanogaster / Laboratory Manuals Molecular Biology / methods Models, Animal Drosophila melanogaster Laboratory Manuals Models, Animal Laboratory Manuals Molecular Biology methods Laboratory Manuals Molecular biology Research Molecular biology Technique Genetik Taufliege Molekularbiologie Methode Aufsatzsammlung
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