The function and regulation of single immunoglobulin IL-1-related receptor (SIGIRR) in kidney disease:
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2007
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| Schlagworte: | |
| Online-Zugang: | kostenfrei https://nbn-resolving.org/urn:nbn:de:bvb:19-78881 Inhaltsverzeichnis |
| Beschreibung: | 133, 11 S. Ill., graph. Darst. |
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| 100 | 1 | |a Lech, Maciej Marcin |d 1979- |e Verfasser |0 (DE-588)134113764 |4 aut | |
| 245 | 1 | 0 | |a The function and regulation of single immunoglobulin IL-1-related receptor (SIGIRR) in kidney disease |c von Maciej Marcin Lech |
| 264 | 1 | |c 2007 | |
| 300 | |a 133, 11 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a München, Univ., Diss., 2007 | ||
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| 912 | |a ebook | ||
| 999 | |a oai:aleph.bib-bvb.de:BVB01-016450659 | ||
Datensatz im Suchindex
| _version_ | 1804137576453373952 |
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| adam_text | 10
TABLE OF CONTENTS
1. INTRODUCTION
1.1. Innate and adaptive immune system
1.2. TLR/IL-1R superfamily signaling
1.3. Regulation of TLR/IL-1R superfamily signaling
1.4. Structure and function of SIGIRR
1.5. Aim of the research project
2. MATERIAL METHODS
2.1. Molecular biology methods
2.1.1. Cloning of human and murine SIGIRR
2.1.2. Electrophoresis of DNA
2.1.3. Ligation of DNA fragments
2.1.4. Generation of competent cells for electro-transformation
2.1.5. Transformation of bacterial cells by electroporation
2.1.6. Small scale plasmid preparation - mini prep
2.1.7. Analysis of DNA with restriction enzymes
2.1.8. RNA isolation from cultured cells
2.1.9. Isolation of RNA from tissues
2.1.10. cDNA synthesis and the real-time PCR analysis
2.1.11. Northern blot
2.2. Cell culture methods and cytokine detection
2.2.1. Cell lines
2.2.2. Calcium phosphate transfection
2.2.3. Reporter gene analysis
2.2.4. Primary cell culture - tubular epithelial cells
2.2.5. Primary cell culture - mesangial cells
11
2.2.6. Primary cell culture - adherent spleen monocytes
2.2.7. Primary cell culture - bone marrow-derived dendritic cells
2.2.8. Primary cell culture - renal antigen presenting cells
2.2.9. Stimulation experiments
2.2.10. Cytokine measurements
2.2.11. Proliferation assay
2.2.12. Phogocytosis assay
2.3. Protein analysis
2.3.1. Western blot
2.3.2. Tissue immunostaining
2.3.4. Immunostaining of cultured cells for SIGIRR
2.2.5. Immunostaining for nuclear p50
2.2.6. Flow cytometry
2.4. In vivo experiments and statistic
2.4.1. Animal studies
2.4.2. Serum IgGs and urine protein measurement
2.4.3. Statistical analysis
3. RESULTS
3.1. Expression of SIGIRR
3.1.1. SIGIRR expression is strain and gender dependent
3.1.2. SIGIRR expression in solid organs
3.1.3. SIGIRR is expressed at high levels in intrarenal myeloid cells and tubular epithelial
cells
3.2. Regulatory function of SIGIRR
3.2.1. Cloning of SIGIRR
3.2.2. SIGIRR inhibits TLR-ligands mediated NF-kB activation
12
3.2.3. SIGIRR is a negative regulator of TLR/IL-1R signalling in immune cells but not in
tubular epithelial cells
3.2.4. SIGIRR is a suppressor of TLR mRNA expression in monocytes but not in tubular
epithelial cells
3.2.5. SIGIRR is a suppressor of TLR mRNA expression in dendritic cells
3.2.6. SIGIRR is a suppressor of TLR signaling in dendritic cells
3.3. Regulation of SIGIRR expression
3.3.1. The regulation of SIGIRR is cell type-specific
3.3.2. Immune cells and renal tubular epithelial cells both express full length SIGIRR
3.4. Posttranslational modification of SIGIRR
3.4.1. SIGIRR glycosylation is cell specific
3.5. Other functions of SIGIRR
3.5.1. SIGIRR influence the maturation of the intrarenal myeloid cells
3.5.2. SIGIRR do not influence the proliferation of tubular epithelial and antigen presenting
cells
3.5.3. The function of SIGIRR during the stress conditions
3.5.4. SIGIRR is not a cell-cell contact molecule
3.6. The role of SIGIRR in systemic lupus erythematosus
3.6.1. Characterization of the C57BL/6 Ipr/lpr mice
3.6.2. SIGIRR affects immune complexes-dependent DCs activation
3.6.3. SIGIRR negatively regulates cytokine and anti dsDNA IgGs in C57BL/6 Ipr/lpr mice
3.6.4. Further abnormalities in C57BL/6 Ipr/lpr SIGIRR-/- mice
4. DISCUSSION SUMMARY
4.1. Function of SIGIRR in the kidney
4.2. Function of SIGIRR in systemic autoimmunity
5. REFERENCES
13
6. CURRICULUM VITAE LIST OF PUBLICATIONS
|
| adam_txt |
10
TABLE OF CONTENTS
1. INTRODUCTION
1.1. Innate and adaptive immune system
1.2. TLR/IL-1R superfamily signaling
1.3. Regulation of TLR/IL-1R superfamily signaling
1.4. Structure and function of SIGIRR
1.5. Aim of the research project
2. MATERIAL METHODS
2.1. Molecular biology methods
2.1.1. Cloning of human and murine SIGIRR
2.1.2. Electrophoresis of DNA
2.1.3. Ligation of DNA fragments
2.1.4. Generation of competent cells for electro-transformation
2.1.5. Transformation of bacterial cells by electroporation
2.1.6. Small scale plasmid preparation - mini prep
2.1.7. Analysis of DNA with restriction enzymes
2.1.8. RNA isolation from cultured cells
2.1.9. Isolation of RNA from tissues
2.1.10. cDNA synthesis and the real-time PCR analysis
2.1.11. Northern blot
2.2. Cell culture methods and cytokine detection
2.2.1. Cell lines
2.2.2. Calcium phosphate transfection
2.2.3. Reporter gene analysis
2.2.4. Primary cell culture - tubular epithelial cells
2.2.5. Primary cell culture - mesangial cells
11
2.2.6. Primary cell culture - adherent spleen monocytes
2.2.7. Primary cell culture - bone marrow-derived dendritic cells
2.2.8. Primary cell culture - renal antigen presenting cells
2.2.9. Stimulation experiments
2.2.10. Cytokine measurements
2.2.11. Proliferation assay
2.2.12. Phogocytosis assay
2.3. Protein analysis
2.3.1. Western blot
2.3.2. Tissue immunostaining
2.3.4. Immunostaining of cultured cells for SIGIRR
2.2.5. Immunostaining for nuclear p50
2.2.6. Flow cytometry
2.4. In vivo experiments and statistic
2.4.1. Animal studies
2.4.2. Serum IgGs and urine protein measurement
2.4.3. Statistical analysis
3. RESULTS
3.1. Expression of SIGIRR
3.1.1. SIGIRR expression is strain and gender dependent
3.1.2. SIGIRR expression in solid organs
3.1.3. SIGIRR is expressed at high levels in intrarenal myeloid cells and tubular epithelial
cells
3.2. Regulatory function of SIGIRR
3.2.1. Cloning of SIGIRR
3.2.2. SIGIRR inhibits TLR-ligands mediated NF-kB activation
12
3.2.3. SIGIRR is a negative regulator of TLR/IL-1R signalling in immune cells but not in
tubular epithelial cells
3.2.4. SIGIRR is a suppressor of TLR mRNA expression in monocytes but not in tubular
epithelial cells
3.2.5. SIGIRR is a suppressor of TLR mRNA expression in dendritic cells
3.2.6. SIGIRR is a suppressor of TLR signaling in dendritic cells
3.3. Regulation of SIGIRR expression
3.3.1. The regulation of SIGIRR is cell type-specific
3.3.2. Immune cells and renal tubular epithelial cells both express full length SIGIRR
3.4. Posttranslational modification of SIGIRR
3.4.1. SIGIRR glycosylation is cell specific
3.5. Other functions of SIGIRR
3.5.1. SIGIRR influence the maturation of the intrarenal myeloid cells
3.5.2. SIGIRR do not influence the proliferation of tubular epithelial and antigen presenting
cells
3.5.3. The function of SIGIRR during the stress conditions
3.5.4. SIGIRR is not a cell-cell contact molecule
3.6. The role of SIGIRR in systemic lupus erythematosus
3.6.1. Characterization of the C57BL/6 Ipr/lpr mice
3.6.2. SIGIRR affects immune complexes-dependent DCs activation
3.6.3. SIGIRR negatively regulates cytokine and anti dsDNA IgGs in C57BL/6 Ipr/lpr mice
3.6.4. Further abnormalities in C57BL/6 Ipr/lpr SIGIRR-/- mice
4. DISCUSSION SUMMARY
4.1. Function of SIGIRR in the kidney
4.2. Function of SIGIRR in systemic autoimmunity
5. REFERENCES
13
6. CURRICULUM VITAE LIST OF PUBLICATIONS |
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| spelling | Lech, Maciej Marcin 1979- Verfasser (DE-588)134113764 aut The function and regulation of single immunoglobulin IL-1-related receptor (SIGIRR) in kidney disease von Maciej Marcin Lech 2007 133, 11 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier München, Univ., Diss., 2007 (DE-588)4113937-9 Hochschulschrift gnd-content Erscheint auch als Online-Ausgabe urn:nbn:de:bvb:19-78881 http://edoc.ub.uni-muenchen.de/7888/ Verlag kostenfrei Volltext https://nbn-resolving.org/urn:nbn:de:bvb:19-78881 Resolving-System HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016450659&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Lech, Maciej Marcin 1979- The function and regulation of single immunoglobulin IL-1-related receptor (SIGIRR) in kidney disease |
| subject_GND | (DE-588)4113937-9 |
| title | The function and regulation of single immunoglobulin IL-1-related receptor (SIGIRR) in kidney disease |
| title_auth | The function and regulation of single immunoglobulin IL-1-related receptor (SIGIRR) in kidney disease |
| title_exact_search | The function and regulation of single immunoglobulin IL-1-related receptor (SIGIRR) in kidney disease |
| title_exact_search_txtP | The function and regulation of single immunoglobulin IL-1-related receptor (SIGIRR) in kidney disease |
| title_full | The function and regulation of single immunoglobulin IL-1-related receptor (SIGIRR) in kidney disease von Maciej Marcin Lech |
| title_fullStr | The function and regulation of single immunoglobulin IL-1-related receptor (SIGIRR) in kidney disease von Maciej Marcin Lech |
| title_full_unstemmed | The function and regulation of single immunoglobulin IL-1-related receptor (SIGIRR) in kidney disease von Maciej Marcin Lech |
| title_short | The function and regulation of single immunoglobulin IL-1-related receptor (SIGIRR) in kidney disease |
| title_sort | the function and regulation of single immunoglobulin il 1 related receptor sigirr in kidney disease |
| topic_facet | Hochschulschrift |
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