Mesenchymal stem cells: methods and protocols
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2008
|
| Schriftenreihe: | Methods in molecular biology
449 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Includes bibliographical references and index Part I. Isolation of human multipotential stromal cells from bone marrow and adipose tissue. Isolation and culture of bone marrow-derived human multipotent stromal cells (hMSCs) / Margaret Wolfe ... [et al.] -- Mesenchymal stem cells from adult bone marrow / Mark F. Pittenger -- A method to isolate and purify human bone marrow stromal stem cells / Stan Gronthos and Andrew C. W. Zannettino -- Adipose-derived stem cells / John K. Fraser ... [et al.] -- Isolation of human adipose-derived stem cells from biopsies and liposuction specimens / Severine G. Dubois ... [et al.] -- Part II. Manipulation of human multipotential stromal cells. Colony forming unit assays for MSCs / Radhika Pochampally -- Differentiation and characterization of human MSCs / Roxanne L. Reger, Alan H. Tucker, and Margaret R. Wolfe -- Freezing harvested hMSCs and recovery of hMSCs from frozen vials for subsequent expansion, analysis, and experimentation / Roxanne L. Reger and Margaret R. Wolfe -- Gene expression analysis at the single cell level using the human bone marrow stromal cell as a model : sample preparation methods / Beerelli Seshi -- Assays of MSCs with microarrays / Joni Yöstalo, Radhika Pochampally, and Darwin J. Prochop -- Gene delivery to mesenchymal stem cells / Reza Izadpanah and Bruce A. Bunnell -- Part III. Isolation and characterization of murine multipotential stromal cells from bone marrow. Isolation of mesenchymal stem cells from murine bone marrow by immunodepletion / Donald G. Phinney |
| Beschreibung: | XVI, 192 S. Ill., graph. Darst. 25 cm |
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| 264 | 1 | |a Totowa, NJ |b Humana Press |c 2008 | |
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| 490 | 1 | |a Methods in molecular biology |v 449 | |
| 500 | |a Includes bibliographical references and index | ||
| 500 | |a Part I. Isolation of human multipotential stromal cells from bone marrow and adipose tissue. Isolation and culture of bone marrow-derived human multipotent stromal cells (hMSCs) / Margaret Wolfe ... [et al.] -- Mesenchymal stem cells from adult bone marrow / Mark F. Pittenger -- A method to isolate and purify human bone marrow stromal stem cells / Stan Gronthos and Andrew C. W. Zannettino -- Adipose-derived stem cells / John K. Fraser ... [et al.] -- Isolation of human adipose-derived stem cells from biopsies and liposuction specimens / Severine G. Dubois ... [et al.] -- Part II. Manipulation of human multipotential stromal cells. Colony forming unit assays for MSCs / Radhika Pochampally -- Differentiation and characterization of human MSCs / Roxanne L. Reger, Alan H. Tucker, and Margaret R. Wolfe -- Freezing harvested hMSCs and recovery of hMSCs from frozen vials for subsequent expansion, analysis, and experimentation / Roxanne L. Reger and Margaret R. Wolfe -- Gene expression analysis at the single cell level using the human bone marrow stromal cell as a model : sample preparation methods / Beerelli Seshi -- Assays of MSCs with microarrays / Joni Yöstalo, Radhika Pochampally, and Darwin J. Prochop -- Gene delivery to mesenchymal stem cells / Reza Izadpanah and Bruce A. Bunnell -- Part III. Isolation and characterization of murine multipotential stromal cells from bone marrow. Isolation of mesenchymal stem cells from murine bone marrow by immunodepletion / Donald G. Phinney | ||
| 650 | 4 | |a Stem cells | |
| 650 | 4 | |a Mesenchymal Stem Cells | |
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| 650 | 2 | |a Techniques cytologiques | |
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| 650 | 4 | |a Mesenchymal Stem Cells |x cytology | |
| 650 | 4 | |a Multipotent Stem Cells |x cytology | |
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Datensatz im Suchindex
| _version_ | 1804137552879288320 |
|---|---|
| adam_text | Contents
Preface v
Contributors xi
Color Plates xv
Part I Isolation of Human Multipotential Stromal Cells
from Bone Marrow and Adipose Tissue
Chapter 1 Isolation and Culture of Bone Marrow-Derived
Human Multipotent Stromal Cells (hMSCs) 3
Margaret Wolfe, Radhika Pochampally, William Swaney,
and Roxanne L. Reger
Chapter 2 Mesenchymal Stem Cells from Adult Bone Marrow 27
Mark F. Pittenger
Chapter 3 A Method to Isolate and Purify Human Bone
Marrow Stromal Stem Cells 45
Stan Gronthos and Andrew C. W. Zannettino
Chapter 4 Adipose-Derived Stem Cells 59
John K. Fraser, Min Zhu, Isabella Wulur, and Zeni Alfonso
Chapter 5 Isolation of Human Adipose-derived Stem Cells
from Biopsies and Liposuction Specimens 69
Severine G. Dubois, Elizabeth Z. Floyd, Sanjin Zvonic,
Gail Kilroy, Xiying Wu, Stacy Carling, Yuan Di C. Halvorsen,
Eric Ravussin, and Jeffrey M. Gimble
Part II Manipulation of Human Multipotential Stromal Cells
Chapter 6 Colony Forming Unit Assays for MSCs 83
Radhika Pochampally
ix
x Contents
Chapter 7 Differentiation and Characterization
of Human MSCs 93
Roxanne L. Reger, Alan H. Tucker, and Margaret R. Wolfe
Chapter 8 Freezing Harvested hMSCs and Recovery of hMSCs
from Frozen Vials for Subsequent Expansion,
Analysis, and Experimentation 109
Roxanne L. Reger and Margaret R. Wolfe
Chapter 9 Gene Expression Analysis at the Single Cell Level
Using the Human Bone Marrow Stromal Cell as a Model:
Sample Preparation Methods 117
Beerelli Seshi
Chapter 10 Assays of MSCs with Microarrays 133
Joni Ylostalo, Radhika Pochampally.
and Darwin J. Prockop
Chapter 11 Gene Delivery to Mesenchymal Stem Cells 153
Reza Izadpanah and Bruce A. Bunnell
Part III Isolation and Characterization of Murine Multipotential
Stromal Cells from Bone Marrow
Chapter 12 Isolation of Mesenchymal Stem Cells from Murine
Bone Marrow by Immunodepletion 171
Donald G. Phinney
Index 187
|
| adam_txt |
Contents
Preface v
Contributors xi
Color Plates xv
Part I Isolation of Human Multipotential Stromal Cells
from Bone Marrow and Adipose Tissue
Chapter 1 Isolation and Culture of Bone Marrow-Derived
Human Multipotent Stromal Cells (hMSCs) 3
Margaret Wolfe, Radhika Pochampally, William Swaney,
and Roxanne L. Reger
Chapter 2 Mesenchymal Stem Cells from Adult Bone Marrow 27
Mark F. Pittenger
Chapter 3 A Method to Isolate and Purify Human Bone
Marrow Stromal Stem Cells 45
Stan Gronthos and Andrew C. W. Zannettino
Chapter 4 Adipose-Derived Stem Cells 59
John K. Fraser, Min Zhu, Isabella Wulur, and Zeni Alfonso
Chapter 5 Isolation of Human Adipose-derived Stem Cells
from Biopsies and Liposuction Specimens 69
Severine G. Dubois, Elizabeth Z. Floyd, Sanjin Zvonic,
Gail Kilroy, Xiying Wu, Stacy Carling, Yuan Di C. Halvorsen,
Eric Ravussin, and Jeffrey M. Gimble
Part II Manipulation of Human Multipotential Stromal Cells
Chapter 6 Colony Forming Unit Assays for MSCs 83
Radhika Pochampally
ix
x Contents
Chapter 7 Differentiation and Characterization
of Human MSCs 93
Roxanne L. Reger, Alan H. Tucker, and Margaret R. Wolfe
Chapter 8 Freezing Harvested hMSCs and Recovery of hMSCs
from Frozen Vials for Subsequent Expansion,
Analysis, and Experimentation 109
Roxanne L. Reger and Margaret R. Wolfe
Chapter 9 Gene Expression Analysis at the Single Cell Level
Using the Human Bone Marrow Stromal Cell as a Model:
Sample Preparation Methods 117
Beerelli Seshi
Chapter 10 Assays of MSCs with Microarrays 133
Joni Ylostalo, Radhika Pochampally.
and Darwin J. Prockop
Chapter 11 Gene Delivery to Mesenchymal Stem Cells 153
Reza Izadpanah and Bruce A. Bunnell
Part III Isolation and Characterization of Murine Multipotential
Stromal Cells from Bone Marrow
Chapter 12 Isolation of Mesenchymal Stem Cells from Murine
Bone Marrow by Immunodepletion 171
Donald G. Phinney
Index 187 |
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| spelling | Mesenchymal stem cells methods and protocols ed. by Darwin J. Prockop ... Totowa, NJ Humana Press 2008 XVI, 192 S. Ill., graph. Darst. 25 cm txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 449 Includes bibliographical references and index Part I. Isolation of human multipotential stromal cells from bone marrow and adipose tissue. Isolation and culture of bone marrow-derived human multipotent stromal cells (hMSCs) / Margaret Wolfe ... [et al.] -- Mesenchymal stem cells from adult bone marrow / Mark F. Pittenger -- A method to isolate and purify human bone marrow stromal stem cells / Stan Gronthos and Andrew C. W. Zannettino -- Adipose-derived stem cells / John K. Fraser ... [et al.] -- Isolation of human adipose-derived stem cells from biopsies and liposuction specimens / Severine G. Dubois ... [et al.] -- Part II. Manipulation of human multipotential stromal cells. Colony forming unit assays for MSCs / Radhika Pochampally -- Differentiation and characterization of human MSCs / Roxanne L. Reger, Alan H. Tucker, and Margaret R. Wolfe -- Freezing harvested hMSCs and recovery of hMSCs from frozen vials for subsequent expansion, analysis, and experimentation / Roxanne L. Reger and Margaret R. Wolfe -- Gene expression analysis at the single cell level using the human bone marrow stromal cell as a model : sample preparation methods / Beerelli Seshi -- Assays of MSCs with microarrays / Joni Yöstalo, Radhika Pochampally, and Darwin J. Prochop -- Gene delivery to mesenchymal stem cells / Reza Izadpanah and Bruce A. Bunnell -- Part III. Isolation and characterization of murine multipotential stromal cells from bone marrow. Isolation of mesenchymal stem cells from murine bone marrow by immunodepletion / Donald G. Phinney Stem cells Mesenchymal Stem Cells Cellules souches mésenchymateuses Cellules souches ram Techniques cytologiques Cell Culture Techniques Cells, Cultured Cytological Techniques Mesenchymal Stem Cells cytology Multipotent Stem Cells cytology Stem cells Laboratory manuals Stromal Cells cytology Biologisches Verfahren (DE-588)4361950-2 gnd rswk-swf Mesenchymzelle (DE-588)4278308-2 gnd rswk-swf Stammzelle (DE-588)4665329-6 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Mesenchymzelle (DE-588)4278308-2 s Stammzelle (DE-588)4665329-6 s Biologisches Verfahren (DE-588)4361950-2 s b DE-604 Prockop, Darwin J. Sonstige oth Methods in molecular biology 449 (DE-604)BV035362695 449 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016435619&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Mesenchymal stem cells methods and protocols Methods in molecular biology Stem cells Mesenchymal Stem Cells Cellules souches mésenchymateuses Cellules souches ram Techniques cytologiques Cell Culture Techniques Cells, Cultured Cytological Techniques Mesenchymal Stem Cells cytology Multipotent Stem Cells cytology Stem cells Laboratory manuals Stromal Cells cytology Biologisches Verfahren (DE-588)4361950-2 gnd Mesenchymzelle (DE-588)4278308-2 gnd Stammzelle (DE-588)4665329-6 gnd |
| subject_GND | (DE-588)4361950-2 (DE-588)4278308-2 (DE-588)4665329-6 (DE-588)4143413-4 |
| title | Mesenchymal stem cells methods and protocols |
| title_auth | Mesenchymal stem cells methods and protocols |
| title_exact_search | Mesenchymal stem cells methods and protocols |
| title_exact_search_txtP | Mesenchymal stem cells methods and protocols |
| title_full | Mesenchymal stem cells methods and protocols ed. by Darwin J. Prockop ... |
| title_fullStr | Mesenchymal stem cells methods and protocols ed. by Darwin J. Prockop ... |
| title_full_unstemmed | Mesenchymal stem cells methods and protocols ed. by Darwin J. Prockop ... |
| title_short | Mesenchymal stem cells |
| title_sort | mesenchymal stem cells methods and protocols |
| title_sub | methods and protocols |
| topic | Stem cells Mesenchymal Stem Cells Cellules souches mésenchymateuses Cellules souches ram Techniques cytologiques Cell Culture Techniques Cells, Cultured Cytological Techniques Mesenchymal Stem Cells cytology Multipotent Stem Cells cytology Stem cells Laboratory manuals Stromal Cells cytology Biologisches Verfahren (DE-588)4361950-2 gnd Mesenchymzelle (DE-588)4278308-2 gnd Stammzelle (DE-588)4665329-6 gnd |
| topic_facet | Stem cells Mesenchymal Stem Cells Cellules souches mésenchymateuses Cellules souches Techniques cytologiques Cell Culture Techniques Cells, Cultured Cytological Techniques Mesenchymal Stem Cells cytology Multipotent Stem Cells cytology Stem cells Laboratory manuals Stromal Cells cytology Biologisches Verfahren Mesenchymzelle Stammzelle Aufsatzsammlung |
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