Cell imaging:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Bloxham
Scion Publ.
2006
|
| Ausgabe: | 1. publ. |
| Schriftenreihe: | Methods express series
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XXX, 303 S. Ill., graph. Darst. |
| ISBN: | 1904842046 9781904842040 1904842267 |
Internformat
MARC
| LEADER | 00000nam a2200000zc 4500 | ||
|---|---|---|---|
| 001 | BV023236939 | ||
| 003 | DE-604 | ||
| 005 | 20080429 | ||
| 007 | t | ||
| 008 | 080403s2006 xxkad|| |||| 00||| eng d | ||
| 010 | |a 2006389019 | ||
| 020 | |a 1904842046 |9 1-904842-04-6 | ||
| 020 | |a 9781904842040 |c pbk. |9 978-1-904842-04-0 | ||
| 020 | |a 1904842267 |9 1-904842-26-7 | ||
| 035 | |a (OCoLC)65516942 | ||
| 035 | |a (DE-599)BVBBV023236939 | ||
| 040 | |a DE-604 |b ger |e aacr | ||
| 041 | 0 | |a eng | |
| 044 | |a xxk |c GB | ||
| 049 | |a DE-355 |a DE-526 | ||
| 050 | 0 | |a QH585 | |
| 082 | 0 | |a 571.6028/4 |2 22 | |
| 084 | |a WC 5100 |0 (DE-625)148108:13423 |2 rvk | ||
| 245 | 1 | 0 | |a Cell imaging |c ed. by D. Stephens |
| 250 | |a 1. publ. | ||
| 264 | 1 | |a Bloxham |b Scion Publ. |c 2006 | |
| 300 | |a XXX, 303 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 0 | |a Methods express series | |
| 650 | 7 | |a Beeldverwerking |2 gtt | |
| 650 | 7 | |a Cellen (biologie) |2 gtt | |
| 650 | 4 | |a Cells |v Laboratory Manuals | |
| 650 | 4 | |a Cytological Techniques |x methods |v Laboratory Manuals | |
| 650 | 4 | |a Cytology |x Technique | |
| 650 | 4 | |a Imaging systems in biology | |
| 650 | 4 | |a Microscopy | |
| 650 | 4 | |a Microscopy |x methods |v Laboratory Manuals | |
| 650 | 0 | 7 | |a Cytologie |0 (DE-588)4070177-3 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Mikroskopie |0 (DE-588)4039238-7 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Bildgebendes Verfahren |0 (DE-588)4006617-4 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4143413-4 |a Aufsatzsammlung |2 gnd-content | |
| 689 | 0 | 0 | |a Cytologie |0 (DE-588)4070177-3 |D s |
| 689 | 0 | 1 | |a Bildgebendes Verfahren |0 (DE-588)4006617-4 |D s |
| 689 | 0 | |5 DE-604 | |
| 689 | 1 | 0 | |a Cytologie |0 (DE-588)4070177-3 |D s |
| 689 | 1 | 1 | |a Mikroskopie |0 (DE-588)4039238-7 |D s |
| 689 | 1 | |C b |5 DE-604 | |
| 700 | 1 | |a Stephens, David |e Sonstige |4 oth | |
| 856 | 4 | 2 | |m GBV Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016422547&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-016422547 | ||
Datensatz im Suchindex
| _version_ | 1804137532478193664 |
|---|---|
| adam_text | EDITED BY D. STEPHENS DEPARTMENT OF BIOCHEMISTRY, UNIVERSITY OF BRISTOL,
BRISTOL, UK SCION CONTENTS CONTRIBUTORS PREFACE ABBREVIATIONS COLOR
SECTION CHAPTER 1 AN INTRODUCTION TO CELL IMAGING DAVID STEPHENS 1.
INTRODUCTION 2. FLUORESCENCE MICROSCOPY 3. GENETICALLY ENCODED
FLUOROPHORES 4. BASIC NECESSITIES - CULTURING CELLS FOR IMAGING 5.
LIMITS OF RESOLUTION AND DETECTION 6. SELECTIVE IMAGING - OPTICAL AND
MECHANICAL 7. CHOICE OF SYSTEM 8. IMAGE PROCESSING AND PRESENTATION 9. A
WORKED EXAMPLE 10. FURTHER RESOURCES 11. REFERENCES CHAPTER 2 CONFOCAL
OR WIDE-FIELD? A GUIDE TO SELECTING APPROPRIATE METHODS IMAGING MARK A.
JEPSON 1. INTRODUCTION 2. METHODS AND APPROACHES 2.1. COMPARISON OF CLSM
AND WFM 2.2. OPTIMIZING IMAGING PARAMETERS: THE NEED FOR COMPROMISE 2.3.
FLEXIBILITY OF POINT-SCANNING SYSTEMS FOR SPECIALIZED IMAGING
APPLICATIONS 2.4. MULTI-COLOR IMAGING 2.5. IMPROVING SPATIAL ACCURACY OF
WFM IMAGES BY DECONVOLUTION 2.6. POTENTIAL PROBLEMS AND NITFALLS IN
WFM/DECONVOLUTION XI XIII XIV XVII 1 2 2 8 8 11 11 12 13 14 15 FOR CELL
17 19 19 20 32 33 34 37 VI * CONTENTS 2.7. IMAGING SYSTEMS WITH ENHANCED
PERFORMANCE 39 2.8. CONCLUDING REMARKS 42 3. RECOMMENDED PROTOCOLS 43
4. REFERENCES 47 CHAPTER 3 IMAGE QUANTIFICATION AND ANALYSIS KOTA MIURA
AND JENS RIETDORF 1. INTRODUCTION 49 2. METHODS AND APPROACHES 50 2.1.
DENSITOMETRY 50 2.2. MORPHOMETRY 51 2.3. MEASUREMENT OF MOVEMENT 52 3.
RECOMMENDED PROTOCOLS 61 4. TROUBLESHOOTING 66 4.1. PROBABLE SOURCES OF
ERROR IN MEASUREMENTS 66 5. REFERENCES 67 6. APPENDIX 69 6.1. GAUSSIAN
FITTING METHOD 69 6.2. PATTERN MATCHING METHOD 70 CHAPTER 4 TRANSMITTED
LIGHT IMAGING JON D. LANE AND HOWARD STEBBINGS 1. INTRODUCTION 73 2.
METHODS AND APPROACHES 74 2.1. THE LIGHT MICROSCOPE OPTICAL SYSTEM AND
BRIGHT-FIELD MICROSCOPY 74 2.2. PHASE CONTRAST MICROSCOPY 75 2.3.
DIFFERENTIAL INTERFERENCE CONTRAST (DIC) MICROSCOPY 78 2.4. POLARIZING
MICROSCOPY 83 2.5. DARK-FIELD MICROSCOPY 84 2.6. AUTORADIOGRAPHY AND THE
BRIGHT-FIELD MICROSCOPE 85 2.7. OTHER TRANSMITTED LIGHT MICROSCOPY
TECHNIQUES 86 3. RECOMMENDED PROTOCOLS 87 4. TROUBLESHOOTING 92 5.
REFERENCES 92 CHAPTER 5 SPECTRAL IMAGING TECHNIQUES FOR FLUORESCENCE
MICROSCOPY TIMO ZIMMERMANN 1. INTRODUCTION 95 1.1 DEFINITION OF THE
OVERLAP OF FLUOROPHORE SIGNALS (CROSSTALK) 96 2. METHODS AND APPROACHES
99 2.1. SPECTRAL IMAGING 99 2.2. LINEAR UNMIXING 99 2.3. METHODS FOR
SPECTRAL IMAGING AND LINEAR UNMIXING 103 CONTENTS * VII 2.4. SPECTRAL
UNMIXING BY SUBTRACTION 105 2.5. APPLICATIONS 106 3. RECOMMENDED
PROTOCOLS 108 4. OPTIMIZATION AND TROUBLESHOOTING 111 4.1. PARAMETERS
AFFECTING SPECTRAL IMAGING AND LINEAR UNMIXING 111 4.2. OPTIMIZATION OF
LINEAR UNMIXING CALCULATIONS 115 5. REFERENCES 116 6. APPENDIX 117 6.1.
NUMERICAL RECIPE FOR LINEAR UNMIXING 117 6.2. LINKS FOR LINEAR UNMIXING
TOOLS 118 CHAPTER 6 MEASUREMENT OF PROTEIN MOTION BY PHOTOBLEACHING JOHN
F. PRESLEY 1. INTRODUCTION 119 1.1. PHYSICS OF PHOTOBLEACHING 120 1.2.
MOTIVATION FOR PHOTOBLEACHING EXPERIMENTS 120 1.3. SCOPE 121 2. METHODS
AND APPROACHES 121 2.1. DIFFUSION OF A FLUORESCENTLY TAGGED PROTEIN
WITHIN A MEMBRANE 121 2.2. DETERMINATION OF DIFFUSION COEFFICIENTS FOR
CYTOPLASMIC PROTEINS 123 2.3. USE OF THE DIFFUSION COEFFICIENT TO DETECT
ENTRY OF A PROTEIN INTO A COMPLEX 123 2.4. FLUORESCENCE LOSS IN
PHOTOBLEACHING (FLIP) 124 2.5. PHOTOACTIVATION 124 2.6. EXCHANGE OF A
PROTEIN BETWEEN A LARGE ORGANELLE AND THE CYTOPLASM 125 2.7. QUALITATIVE
ANALYSIS OF EXCHANGE BETWEEN AN ORGANELLE AND THE REST OF THE CELL 125
2.8. PHOTOBLEACHING OF FRAGMENTED ORGANELLES 128 2.9. ANALOGOUS PROBLEMS
131 2.10. MORE COMPLEX KINETIC MODELS 132 3. RECOMMENDED PROTOCOLS 132
4. TROUBLESHOOTING 139 4.1. FOCUS PROBLEMS 139 4.2. PHOTOBLEACHING
DURING SEQUENCE ACQUISITION 140 4.3. DIFFICULT CURVE FITS 140 4.4. OTHER
PROBLEMS 140 5. REFERENCES 141 CHAPTER 7 IMAGING CALCIUM AND
CALCIUM-BINDING PROTEINS BURCU HASDEMIR, ROBERT BURGOYNE AND ALEXEI
TEPIKIN 1. INTRODUCTION 143 2. METHODS AND APPROACHES 143 2.1. IMAGING
CALCIUM IN THE CYTOSOL 143 2.2. HARDWARE FOR DETECTION OF CALCIUM
SIGNALS 147 VIII D CONTENTS 3. 4. 5. 2.3. IMAGING MITOCHONDRIAL 2.4.
IMAGING CALCIUM IN THE 2.5. IMAGING CALCIUM IN THE CALCIUM ER LUMEN
GOLGI - NOT JUST YET 2.6. IMAGING EXTRACELLULAR CALCIUM 2.7. FROM
CALCIUM SIGNALS TO RECOMMENDED PROTOCOLS TROUBLESHOOTING REFERENCES
ICABPS 147 149 151 152 153 155 161 163 CHAPTER 8 TOTAL INTERNAL
REFLECTION FLUORESCENCE MICROSCOPY DAVID ZENISEK 1. INTRODUCTION 165
1.1. GENERAL PROPERTIES OF REFLECTION AND REFRACTION AT AN INTERFACE 165
1.2. PRINCIPLES OF TIRFM 167 1.3. APPLICATIONS OF TIRFM 168 2. METHODS
AND APPROACHES 169 2.1. TECHNIQUES FOR TIRFM 169 3. RECOMMENDED
PROTOCOLS 173 4. REFERENCES 175 CHAPTER 9 AN OVERVIEW OF
THREE-DIMENSIONAL AND FOUR-DIMENSIONAL MICROSCOPY BY COMPUTATIONAL
DECONVOLUTION JOSE-ANGEL CONCHELLO 1. INTRODUCTION 177 1.1. GENERAL
PRINCIPLES 177 2. METHODS AND APPROACHES 179 2.1. SIMPLE MODELS FOR
IMAGE FORMATION 179 2.2 PSEUDO-3D MODELS - NEAREST-NEIGHBORS SUBTRACTION
179 2.3. METHODS BASED ON THE FREQUENCY RESPONSE 180 2.4. CONSTRAINED
DECONVOLUTION 187 2.5. STATISTICAL IMAGE ESTIMATION 189 2.6. BLIND
DECONVOLUTION 192 3. RECOMMENDED PROTOCOLS 193 3.1. ACQUIRING IMAGES TO
CORRECT FOR BACKGROUND AND A NONUNIFORM FLAT-FIELD RESPONSE 193 3.2.
ACQUIRING A SUITABLE IMAGE STACK FOR PSF CALCULATION 194 4. CONCLUSIONS
AND CURRENT RESEARCH 197 5. REFERENCES 198 CHAPTER 10 CORRELATIVE
MICROSCOPY USING TOKUYASU CRYOSECTIONS: APPLICATIONS FOR IMMUNOLABELING
AND IN SITU HYBRIDIZATION MIGUEL R. BRANCO, SHEILA Q. XIE, SONYA MARTIN
AND ANA POMBO 1. INTRODUCTION 201 1.1. ANTIBODY ACCESSIBILITY THROUGH
CRYOSECTIONS 203 2. METHODS AND APPROACHES 2.1. CELL FIXATION,
EMBEDDING, AND CRYOSECTIONING 2.2. IMMUNOLABELING FOR LM AND EM 2.3. RNA
IN SITU HYBRIDIZATION 2.4. CORRELATIVE MICROSCOPY RECOMMENDED PROTOCOLS
REFERENCES CONTENTS * IX 204 204 205 207 207 211 217 3. 4. CHAPTER 11
FLUORESCENCE LIFETIME IMAGING KLAUS SUHLING 1. INTRODUCTION 219 1.1. A
VERY BRIEF HISTORY OF FLUORESCENCE, LIFETIME, AND IMAGING 219 1.2.
FLUORESCENCE MICROSCOPY 221 1.3. WHAT IS FLUORESCENCE? 222 1.4. WHAT IS
FLIM AND WHY IS IT USED? 226 1.5. POLARIZATION-RESOLVED FLIM AND
SPECTRALLY RESOLVED FLIM 226 2. METHODS AND APPROACHES 229 2.1.
FLUORESCENCE IMAGING CONCEPTS 229 2.2. FLIM IMPLEMENTATION 229 3.
RECOMMENDED PROTOCOLS 234 4. DATA ANALYSIS 237 4.1. FREQUENCY DOMAIN 237
4.2. TIME DOMAIN 237 4.3. DECAY MODELS 239 4.4. GLOBAL ANALYSIS 240 4.5.
IMAGE DISPLAY 240 5. TROUBLESHOOTING 241 6. APPLICATIONS OF FLIM 242 7.
OUTLOOK 242 8. REFERENCES 243 CHAPTER 12 HOMO-FRET MEASUREMENTS TO
INVESTIGATE MOLECULAR-SCALE ORGANIZATION OF PROTEINS IN LIVING CELLS
RAJAT VARMA AND SATYAJIT MAYOR 1. INTRODUCTION 247 1.1. THEORETICAL
CONCEPTS 248 2. METHODS AND APPROACHES 250 2.1. EXPERIMENTAL APPROACHES
TO MEASURING MOLECULAR PROXIMITY USING FRET 250 3. RECOMMENDED PROTOCOLS
253 3.1. FLUOROPHORE CHOICE FOR HOMO-FRET MICROSCOPY 253 3.2.
EXPERIMENTAL DESIGN OF HOMO-FRET MEASUREMENTS 254 3.3. MODIFICATIONS OF
WIDE-FIELD FLUORESCENCE MICROSCOPY FOR ANISOTROPY MEASUREMENTS 255 X D
CONTENTS 3.4. SPATIAL RESOLUTION, PIXEL SHIFTS, AND PRECISION 258 3.5.
ANALYSIS 261 3.6. PROTOTYPE MEASUREMENTS 261 3.7. ANALYZING
CO-LOCALIZATION AT THE NANOMETER SCALE 263 3.8. QUANTITATION OF FRET
EFFICIENCIES FROM THE HOMO-FRET EXPERIMENT 264 3.9. COMPARISON OF THE
SENSITIVITIES OF HOMO- AND HETERO-FRET IN BULK SOLUTION 264 3.10.
COMPARISON BETWEEN HOMO-FRET AND HETERO-FRET MEASUREMENTS FOR SMALL
CLUSTERS 265 4. FURTHER IMPROVEMENTS IN ANISOTROPY MEASUREMENTS 265 5.
SUMMARY 266 6. REFERENCES 267 CHAPTER 13 HIGH-CONTENT AND
HIGH-THROUGHPUT SCREENING ELIZABETH P. ROQUEMORE 1. INTRODUCTION 269
1.1. HIGH-CONTENT, HIGH-THROUGHPUT SCREENING FOR PRE-CLINICAL DRUG
DISCOVERY 269 2. METHODS AND APPROACHES 277 2.1. ASSAY DEVELOPMENT 277
2.2. IMAGE ACQUISITION 281 2.3. IMAGE ANALYSIS 282 3. RECOMMENDED
PROTOCOLS 283 3.1. HIGH-THROUGHPUT, CELL-BASED SCREENING FOR GPCR
ANTAGONISTS 283 3.2. HIGH-CONTENT SIRNA SCREENING 287 3.3. HIGH-CONTENT
ANALYSIS DURING COMPOUND PROFILING 287 4. TROUBLESHOOTING 293 5.
REFERENCES 294 APPENDIX 1 LIST OF SUPPLIERS 297 INDEX 301
|
| adam_txt |
EDITED BY D. STEPHENS DEPARTMENT OF BIOCHEMISTRY, UNIVERSITY OF BRISTOL,
BRISTOL, UK SCION CONTENTS CONTRIBUTORS PREFACE ABBREVIATIONS COLOR
SECTION CHAPTER 1 AN INTRODUCTION TO CELL IMAGING DAVID STEPHENS 1.
INTRODUCTION 2. FLUORESCENCE MICROSCOPY 3. GENETICALLY ENCODED
FLUOROPHORES 4. BASIC NECESSITIES - CULTURING CELLS FOR IMAGING 5.
LIMITS OF RESOLUTION AND DETECTION 6. SELECTIVE IMAGING - OPTICAL AND
MECHANICAL 7. CHOICE OF SYSTEM 8. IMAGE PROCESSING AND PRESENTATION 9. A
WORKED EXAMPLE 10. FURTHER RESOURCES 11. REFERENCES CHAPTER 2 CONFOCAL
OR WIDE-FIELD? A GUIDE TO SELECTING APPROPRIATE METHODS IMAGING MARK A.
JEPSON 1. INTRODUCTION 2. METHODS AND APPROACHES 2.1. COMPARISON OF CLSM
AND WFM 2.2. OPTIMIZING IMAGING PARAMETERS: THE NEED FOR COMPROMISE 2.3.
FLEXIBILITY OF POINT-SCANNING SYSTEMS FOR SPECIALIZED IMAGING
APPLICATIONS 2.4. MULTI-COLOR IMAGING 2.5. IMPROVING SPATIAL ACCURACY OF
WFM IMAGES BY DECONVOLUTION 2.6. POTENTIAL PROBLEMS AND NITFALLS IN
WFM/DECONVOLUTION XI XIII XIV XVII 1 2 2 8 8 11 11 12 13 14 15 FOR CELL
17 19 19 20 32 33 34 37 VI * CONTENTS 2.7. IMAGING SYSTEMS WITH ENHANCED
PERFORMANCE 39 2.8. CONCLUDING REMARKS 42 3. RECOMMENDED PROTOCOLS " 43
4. REFERENCES 47 CHAPTER 3 IMAGE QUANTIFICATION AND ANALYSIS KOTA MIURA
AND JENS RIETDORF 1. INTRODUCTION 49 2. METHODS AND APPROACHES 50 2.1.
DENSITOMETRY 50 2.2. MORPHOMETRY 51 2.3. MEASUREMENT OF MOVEMENT 52 3.
RECOMMENDED PROTOCOLS 61 4. TROUBLESHOOTING 66 4.1. PROBABLE SOURCES OF
ERROR IN MEASUREMENTS 66 5. REFERENCES 67 6. APPENDIX 69 6.1. GAUSSIAN
FITTING METHOD 69 6.2. PATTERN MATCHING METHOD 70 CHAPTER 4 TRANSMITTED
LIGHT IMAGING JON D. LANE AND HOWARD STEBBINGS 1. INTRODUCTION 73 2.
METHODS AND APPROACHES 74 2.1. THE LIGHT MICROSCOPE OPTICAL SYSTEM AND
BRIGHT-FIELD MICROSCOPY 74 2.2. PHASE CONTRAST MICROSCOPY 75 2.3.
DIFFERENTIAL INTERFERENCE CONTRAST (DIC) MICROSCOPY 78 2.4. POLARIZING
MICROSCOPY 83 2.5. DARK-FIELD MICROSCOPY 84 2.6. AUTORADIOGRAPHY AND THE
BRIGHT-FIELD MICROSCOPE 85 2.7. OTHER TRANSMITTED LIGHT MICROSCOPY
TECHNIQUES 86 3. RECOMMENDED PROTOCOLS 87 4. TROUBLESHOOTING 92 5.
REFERENCES 92 CHAPTER 5 SPECTRAL IMAGING TECHNIQUES FOR FLUORESCENCE
MICROSCOPY TIMO ZIMMERMANN 1. INTRODUCTION 95 1.1 DEFINITION OF THE
OVERLAP OF FLUOROPHORE SIGNALS (CROSSTALK) 96 2. METHODS AND APPROACHES
99 2.1. SPECTRAL IMAGING 99 2.2. LINEAR UNMIXING 99 2.3. METHODS FOR
SPECTRAL IMAGING AND LINEAR UNMIXING 103 CONTENTS * VII 2.4. SPECTRAL
UNMIXING BY SUBTRACTION 105 2.5. APPLICATIONS 106 3. RECOMMENDED
PROTOCOLS 108 4. OPTIMIZATION AND TROUBLESHOOTING 111 4.1. PARAMETERS
AFFECTING SPECTRAL IMAGING AND LINEAR UNMIXING 111 4.2. OPTIMIZATION OF
LINEAR UNMIXING CALCULATIONS 115 5. REFERENCES 116 6. APPENDIX 117 6.1.
NUMERICAL RECIPE FOR LINEAR UNMIXING 117 6.2. LINKS FOR LINEAR UNMIXING
TOOLS 118 CHAPTER 6 MEASUREMENT OF PROTEIN MOTION BY PHOTOBLEACHING JOHN
F. PRESLEY 1. INTRODUCTION 119 1.1. PHYSICS OF PHOTOBLEACHING 120 1.2.
MOTIVATION FOR PHOTOBLEACHING EXPERIMENTS 120 1.3. SCOPE 121 2. METHODS
AND APPROACHES 121 2.1. DIFFUSION OF A FLUORESCENTLY TAGGED PROTEIN
WITHIN A MEMBRANE 121 2.2. DETERMINATION OF DIFFUSION COEFFICIENTS FOR
CYTOPLASMIC PROTEINS 123 2.3. USE OF THE DIFFUSION COEFFICIENT TO DETECT
ENTRY OF A PROTEIN INTO A COMPLEX 123 2.4. FLUORESCENCE LOSS IN
PHOTOBLEACHING (FLIP) 124 2.5. PHOTOACTIVATION 124 2.6. EXCHANGE OF A
PROTEIN BETWEEN A LARGE ORGANELLE AND THE CYTOPLASM 125 2.7. QUALITATIVE
ANALYSIS OF EXCHANGE BETWEEN AN ORGANELLE AND THE REST OF THE CELL 125
2.8. PHOTOBLEACHING OF FRAGMENTED ORGANELLES 128 2.9. ANALOGOUS PROBLEMS
131 2.10. MORE COMPLEX KINETIC MODELS ' 132 3. RECOMMENDED PROTOCOLS 132
4. TROUBLESHOOTING 139 4.1. FOCUS PROBLEMS 139 4.2. PHOTOBLEACHING
DURING SEQUENCE ACQUISITION 140 4.3. DIFFICULT CURVE FITS 140 4.4. OTHER
PROBLEMS 140 5. REFERENCES 141 CHAPTER 7 IMAGING CALCIUM AND
CALCIUM-BINDING PROTEINS BURCU HASDEMIR, ROBERT BURGOYNE AND ALEXEI
TEPIKIN 1. INTRODUCTION 143 2. METHODS AND APPROACHES 143 2.1. IMAGING
CALCIUM IN THE CYTOSOL 143 2.2. HARDWARE FOR DETECTION OF CALCIUM
SIGNALS 147 VIII D CONTENTS 3. 4. 5. 2.3. IMAGING MITOCHONDRIAL 2.4.
IMAGING CALCIUM IN THE 2.5. IMAGING CALCIUM IN THE CALCIUM ER LUMEN
GOLGI - NOT JUST YET 2.6. IMAGING EXTRACELLULAR CALCIUM 2.7. FROM
CALCIUM SIGNALS TO RECOMMENDED PROTOCOLS TROUBLESHOOTING REFERENCES
ICABPS 147 149 151 152 153 155 161 163 CHAPTER 8 TOTAL INTERNAL
REFLECTION FLUORESCENCE MICROSCOPY DAVID ZENISEK 1. INTRODUCTION 165
1.1. GENERAL PROPERTIES OF REFLECTION AND REFRACTION AT AN INTERFACE 165
1.2. PRINCIPLES OF TIRFM 167 1.3. APPLICATIONS OF TIRFM 168 2. METHODS
AND APPROACHES 169 2.1. TECHNIQUES FOR TIRFM 169 3. RECOMMENDED
PROTOCOLS 173 4. REFERENCES 175 CHAPTER 9 AN OVERVIEW OF
THREE-DIMENSIONAL AND FOUR-DIMENSIONAL MICROSCOPY BY COMPUTATIONAL
DECONVOLUTION JOSE-ANGEL CONCHELLO 1. INTRODUCTION 177 1.1. GENERAL
PRINCIPLES 177 2. METHODS AND APPROACHES 179 2.1. SIMPLE MODELS FOR
IMAGE FORMATION 179 2.2 PSEUDO-3D MODELS - NEAREST-NEIGHBORS SUBTRACTION
179 2.3. METHODS BASED ON THE FREQUENCY RESPONSE 180 2.4. CONSTRAINED
DECONVOLUTION 187 2.5. STATISTICAL IMAGE ESTIMATION 189 2.6. BLIND
DECONVOLUTION 192 3. RECOMMENDED PROTOCOLS 193 3.1. ACQUIRING IMAGES TO
CORRECT FOR BACKGROUND AND A NONUNIFORM FLAT-FIELD RESPONSE 193 3.2.
ACQUIRING A SUITABLE IMAGE STACK FOR PSF CALCULATION 194 4. CONCLUSIONS
AND CURRENT RESEARCH 197 5. REFERENCES 198 CHAPTER 10 CORRELATIVE
MICROSCOPY USING TOKUYASU CRYOSECTIONS: APPLICATIONS FOR IMMUNOLABELING
AND IN SITU HYBRIDIZATION MIGUEL R. BRANCO, SHEILA Q. XIE, SONYA MARTIN
AND ANA POMBO 1. INTRODUCTION 201 1.1. ANTIBODY ACCESSIBILITY THROUGH
CRYOSECTIONS 203 2. METHODS AND APPROACHES 2.1. CELL FIXATION,
EMBEDDING, AND CRYOSECTIONING 2.2. IMMUNOLABELING FOR LM AND EM 2.3. RNA
IN SITU HYBRIDIZATION 2.4. CORRELATIVE MICROSCOPY RECOMMENDED PROTOCOLS
REFERENCES CONTENTS * IX 204 204 205 207 207 211 217 3. 4. CHAPTER 11
FLUORESCENCE LIFETIME IMAGING KLAUS SUHLING 1. INTRODUCTION 219 1.1. A
VERY BRIEF HISTORY OF FLUORESCENCE, LIFETIME, AND IMAGING 219 1.2.
FLUORESCENCE MICROSCOPY 221 1.3. WHAT IS FLUORESCENCE? 222 1.4. WHAT IS
FLIM AND WHY IS IT USED? 226 1.5. POLARIZATION-RESOLVED FLIM AND
SPECTRALLY RESOLVED FLIM 226 2. METHODS AND APPROACHES 229 2.1.
FLUORESCENCE IMAGING CONCEPTS 229 2.2. FLIM IMPLEMENTATION 229 3.
RECOMMENDED PROTOCOLS 234 4. DATA ANALYSIS 237 4.1. FREQUENCY DOMAIN 237
4.2. TIME DOMAIN 237 4.3. DECAY MODELS 239 4.4. GLOBAL ANALYSIS 240 4.5.
IMAGE DISPLAY 240 5. TROUBLESHOOTING 241 6. APPLICATIONS OF FLIM 242 7.
OUTLOOK 242 8. REFERENCES 243 CHAPTER 12 HOMO-FRET MEASUREMENTS TO
INVESTIGATE MOLECULAR-SCALE ORGANIZATION OF PROTEINS IN LIVING CELLS
RAJAT VARMA AND SATYAJIT MAYOR 1. INTRODUCTION 247 1.1. THEORETICAL
CONCEPTS 248 2. METHODS AND APPROACHES 250 2.1. EXPERIMENTAL APPROACHES
TO MEASURING MOLECULAR PROXIMITY USING FRET 250 3. RECOMMENDED PROTOCOLS
253 3.1. FLUOROPHORE CHOICE FOR HOMO-FRET MICROSCOPY 253 3.2.
EXPERIMENTAL DESIGN OF HOMO-FRET MEASUREMENTS 254 3.3. MODIFICATIONS OF
WIDE-FIELD FLUORESCENCE MICROSCOPY FOR ANISOTROPY MEASUREMENTS 255 X D
CONTENTS 3.4. SPATIAL RESOLUTION, PIXEL SHIFTS, AND PRECISION 258 3.5.
ANALYSIS 261 3.6. PROTOTYPE MEASUREMENTS 261 3.7. ANALYZING
CO-LOCALIZATION AT THE NANOMETER SCALE 263 3.8. QUANTITATION OF FRET
EFFICIENCIES FROM THE HOMO-FRET EXPERIMENT 264 3.9. COMPARISON OF THE
SENSITIVITIES OF HOMO- AND HETERO-FRET IN BULK SOLUTION 264 3.10.
COMPARISON BETWEEN HOMO-FRET AND HETERO-FRET MEASUREMENTS FOR SMALL
CLUSTERS 265 4. FURTHER IMPROVEMENTS IN ANISOTROPY MEASUREMENTS 265 5.
SUMMARY 266 6. REFERENCES 267 CHAPTER 13 HIGH-CONTENT AND
HIGH-THROUGHPUT SCREENING ELIZABETH P. ROQUEMORE 1. INTRODUCTION 269
1.1. HIGH-CONTENT, HIGH-THROUGHPUT SCREENING FOR PRE-CLINICAL DRUG
DISCOVERY 269 2. METHODS AND APPROACHES 277 2.1. ASSAY DEVELOPMENT 277
2.2. IMAGE ACQUISITION 281 2.3. IMAGE ANALYSIS 282 3. RECOMMENDED
PROTOCOLS 283 3.1. HIGH-THROUGHPUT, CELL-BASED SCREENING FOR GPCR
ANTAGONISTS 283 3.2. HIGH-CONTENT SIRNA SCREENING 287 3.3. HIGH-CONTENT
ANALYSIS DURING COMPOUND PROFILING 287 4. TROUBLESHOOTING 293 5.
REFERENCES 294 APPENDIX 1 LIST OF SUPPLIERS 297 INDEX 301 |
| any_adam_object | 1 |
| any_adam_object_boolean | 1 |
| building | Verbundindex |
| bvnumber | BV023236939 |
| callnumber-first | Q - Science |
| callnumber-label | QH585 |
| callnumber-raw | QH585 |
| callnumber-search | QH585 |
| callnumber-sort | QH 3585 |
| callnumber-subject | QH - Natural History and Biology |
| classification_rvk | WC 5100 |
| ctrlnum | (OCoLC)65516942 (DE-599)BVBBV023236939 |
| dewey-full | 571.6028/4 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 571 - Physiology & related subjects |
| dewey-raw | 571.6028/4 |
| dewey-search | 571.6028/4 |
| dewey-sort | 3571.6028 14 |
| dewey-tens | 570 - Biology |
| discipline | Biologie |
| discipline_str_mv | Biologie |
| edition | 1. publ. |
| format | Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>02119nam a2200589zc 4500</leader><controlfield tag="001">BV023236939</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20080429 </controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">080403s2006 xxkad|| |||| 00||| eng d</controlfield><datafield tag="010" ind1=" " ind2=" "><subfield code="a">2006389019</subfield></datafield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">1904842046</subfield><subfield code="9">1-904842-04-6</subfield></datafield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9781904842040</subfield><subfield code="c">pbk.</subfield><subfield code="9">978-1-904842-04-0</subfield></datafield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">1904842267</subfield><subfield code="9">1-904842-26-7</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)65516942</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV023236939</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">aacr</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="044" ind1=" " ind2=" "><subfield code="a">xxk</subfield><subfield code="c">GB</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-355</subfield><subfield code="a">DE-526</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">QH585</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">571.6028/4</subfield><subfield code="2">22</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WC 5100</subfield><subfield code="0">(DE-625)148108:13423</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Cell imaging</subfield><subfield code="c">ed. by D. Stephens</subfield></datafield><datafield tag="250" ind1=" " ind2=" "><subfield code="a">1. publ.</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Bloxham</subfield><subfield code="b">Scion Publ.</subfield><subfield code="c">2006</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">XXX, 303 S.</subfield><subfield code="b">Ill., graph. Darst.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="0" ind2=" "><subfield code="a">Methods express series</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Beeldverwerking</subfield><subfield code="2">gtt</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Cellen (biologie)</subfield><subfield code="2">gtt</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Cells</subfield><subfield code="v">Laboratory Manuals</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Cytological Techniques</subfield><subfield code="x">methods</subfield><subfield code="v">Laboratory Manuals</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Cytology</subfield><subfield code="x">Technique</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Imaging systems in biology</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Microscopy</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Microscopy</subfield><subfield code="x">methods</subfield><subfield code="v">Laboratory Manuals</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Cytologie</subfield><subfield code="0">(DE-588)4070177-3</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Mikroskopie</subfield><subfield code="0">(DE-588)4039238-7</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Bildgebendes Verfahren</subfield><subfield code="0">(DE-588)4006617-4</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4143413-4</subfield><subfield code="a">Aufsatzsammlung</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Cytologie</subfield><subfield code="0">(DE-588)4070177-3</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Bildgebendes Verfahren</subfield><subfield code="0">(DE-588)4006617-4</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="5">DE-604</subfield></datafield><datafield tag="689" ind1="1" ind2="0"><subfield code="a">Cytologie</subfield><subfield code="0">(DE-588)4070177-3</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="1" ind2="1"><subfield code="a">Mikroskopie</subfield><subfield code="0">(DE-588)4039238-7</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="1" ind2=" "><subfield code="C">b</subfield><subfield code="5">DE-604</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Stephens, David</subfield><subfield code="e">Sonstige</subfield><subfield code="4">oth</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">GBV Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016422547&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-016422547</subfield></datafield></record></collection> |
| genre | (DE-588)4143413-4 Aufsatzsammlung gnd-content |
| genre_facet | Aufsatzsammlung |
| id | DE-604.BV023236939 |
| illustrated | Illustrated |
| index_date | 2024-07-02T20:22:28Z |
| indexdate | 2024-07-09T21:13:47Z |
| institution | BVB |
| isbn | 1904842046 9781904842040 1904842267 |
| language | English |
| lccn | 2006389019 |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-016422547 |
| oclc_num | 65516942 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-526 |
| owner_facet | DE-355 DE-BY-UBR DE-526 |
| physical | XXX, 303 S. Ill., graph. Darst. |
| publishDate | 2006 |
| publishDateSearch | 2006 |
| publishDateSort | 2006 |
| publisher | Scion Publ. |
| record_format | marc |
| series2 | Methods express series |
| spelling | Cell imaging ed. by D. Stephens 1. publ. Bloxham Scion Publ. 2006 XXX, 303 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods express series Beeldverwerking gtt Cellen (biologie) gtt Cells Laboratory Manuals Cytological Techniques methods Laboratory Manuals Cytology Technique Imaging systems in biology Microscopy Microscopy methods Laboratory Manuals Cytologie (DE-588)4070177-3 gnd rswk-swf Mikroskopie (DE-588)4039238-7 gnd rswk-swf Bildgebendes Verfahren (DE-588)4006617-4 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Cytologie (DE-588)4070177-3 s Bildgebendes Verfahren (DE-588)4006617-4 s DE-604 Mikroskopie (DE-588)4039238-7 s b DE-604 Stephens, David Sonstige oth GBV Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016422547&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Cell imaging Beeldverwerking gtt Cellen (biologie) gtt Cells Laboratory Manuals Cytological Techniques methods Laboratory Manuals Cytology Technique Imaging systems in biology Microscopy Microscopy methods Laboratory Manuals Cytologie (DE-588)4070177-3 gnd Mikroskopie (DE-588)4039238-7 gnd Bildgebendes Verfahren (DE-588)4006617-4 gnd |
| subject_GND | (DE-588)4070177-3 (DE-588)4039238-7 (DE-588)4006617-4 (DE-588)4143413-4 |
| title | Cell imaging |
| title_auth | Cell imaging |
| title_exact_search | Cell imaging |
| title_exact_search_txtP | Cell imaging |
| title_full | Cell imaging ed. by D. Stephens |
| title_fullStr | Cell imaging ed. by D. Stephens |
| title_full_unstemmed | Cell imaging ed. by D. Stephens |
| title_short | Cell imaging |
| title_sort | cell imaging |
| topic | Beeldverwerking gtt Cellen (biologie) gtt Cells Laboratory Manuals Cytological Techniques methods Laboratory Manuals Cytology Technique Imaging systems in biology Microscopy Microscopy methods Laboratory Manuals Cytologie (DE-588)4070177-3 gnd Mikroskopie (DE-588)4039238-7 gnd Bildgebendes Verfahren (DE-588)4006617-4 gnd |
| topic_facet | Beeldverwerking Cellen (biologie) Cells Laboratory Manuals Cytological Techniques methods Laboratory Manuals Cytology Technique Imaging systems in biology Microscopy Microscopy methods Laboratory Manuals Cytologie Mikroskopie Bildgebendes Verfahren Aufsatzsammlung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016422547&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT stephensdavid cellimaging |