Genomics protocols:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2008
|
| Ausgabe: | 2. ed. |
| Schriftenreihe: | Methods in molecular biology
439 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XV, 425 S. graph. Darst. |
| ISBN: | 9781588298713 |
Internformat
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| 650 | 4 | |a Gene mapping |v Laboratory manuals | |
| 650 | 4 | |a Genomics |v Laboratory manuals | |
| 650 | 4 | |a Genomics |x methods |v Laboratory Manuals | |
| 650 | 4 | |a Molecular Biology |v Laboratory Manuals | |
| 650 | 4 | |a Sequence Analysis, DNA |v Laboratory Manuals | |
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Datensatz im Suchindex
| _version_ | 1804137431350378496 |
|---|---|
| adam_text | Contents
Preface v
Contributors xi
Chapter 1 Whole Genome Amplification with Phi29 DNA
Polymerase to Enable Genetic or Genomic Analysis
of Samples of Low DNA Yield 1
Kaisa Silander and Janna Saarela
Chapter 2 Scanning for DNA Variants by Denaturant Capillary
Electrophoresis 19
Per O. Ekstr0m
Chapter 3 Identification of SNPs, or Mutations
in Sequence Chromatograms 35
Nicole Draper
Chapter 4 BeadArray-Based Genotyping 53
Helen Butler and Jiannis Ragoussis
Chapter 5 Microsatellite-Based Candidate Gene Linkage
Analysis Studies 75
Cathryn Mellersh
Chapter 6 Full Complexity Genomic Hybridization on 60-mer
Oligonucleotide Microarrays for Array Comparative
Genomic Hybridization (aCGH) 87
Alexei Protopopov, Bin Feng, and Lynda Chin
Chapter 7 Detection of Copy Number Changes at Multiple Loci in DNA
Prepared from Formalin-Fixed, Paraffin-Embedded Tissue
by Multiplex Ligation-Dependent Probe Amplification .... 101
Minoru Takata
vii
viii Contents
Chapter 8 Application of Microarrays for DNA
Methylation Profiling 109
Axel Schumacher, Andreas Weinhausl, and Arturas Petronis
Chapter 9 Genomewide Identification of Protein Binding
Locations Using Chromatin Immunoprecipitation
Coupled with Microarray 131
Byung-Kwan Cho, Eric M. Knight, and Bernhard 0. Palsson
Chapter 10 Transcriptional Profiling of Small Samples in the
Central Nervous System 147
Stephen D. Ginsberg
Chapter 11 Quantitative Expression Profiling of RNA from
Formalin-Fixed, Paraffin-Embedded Tissues Using
Randomly Assembled Bead Arrays 159
Marina Bibikova, Joanne M. Yeakley,
Jessica Wang-Rodriguez, and Jian-Bing Fan
Chapter 12 Expression Profiling of microRNAs in Cancer Cells:
Technical Considerations 179
Mouldy Sioud and Lina Cekaite
Chapter 13 Identification of Disease Biomarkers by Profiling of Serum
Proteins Using SELDI-TOF Mass Spectrometry 191
Sigrun Langbein
Chapter 14 The Applicability of a Cluster of Differentiation
Monoclonal Antibody Microarray to the
Diagnosis of Human Disease 199
Peter Ellmark, Adrian Woolfson, Larissa Belov, and
Richard I. Christopherson
Chapter 15 Protein Profiling Based on Two-Dimensional
Difference Gel Electrophoresis 211
Gert Van den Bergh and Lutgarde Arckens
Chapter 16 Quantitative Protein Profiling by Mass Spectrometry
Using Isotope-Coded Affinity Tags 225
Arsalan S. Haqqani, John F. Kelly, and Danica B. Stanimirovic
Chapter 17 Quantitative Protein Profiling by Mass Spectrometry
Using Label-Free Proteomics 241
Arsalan S. Haqqani, John F. Kelly, and Danica B. Stanimirovic
I Contents ix
i
j Chapter 18 Using 2D-LC-MS/MS to Identify Francisella tularensis
j Peptides in Extracts from an Infected Mouse
| Macrophage Cell Line 257
j John F. Kelly and Wen Ding
Chapter 19 Baculovirus Expression Vector System:
An Emerging Host for High-Throughput Eukaryotic
Protein Expression 269
Binesh Shrestha, Carol Smee, and Opher Gileadi
Chapter 20 Coimmunoprecipitation and Proteomic Analyses 291
S. Fabio Falsone, Bernd Gesslbauer, and Andreas J. Kungl
Chapter 21 Tandem Affinity Purification Combined with
Mass Spectrometry to Identify Components
of Protein Complexes 309
Peter Kaiser, David Meierhofer, Xiaorong Wang,
and Lan Huang
Chapter 22 Mammalian Two-Hybrid Assay for Detecting
Protein-Protein Interactions in vivo 327
Runtao He and Xuguang Li
Chapter 23 Detection of Protein-Protein Interactions in Live
Cells and Animals with Split Firefly Luciferase
Protein Fragment Complementation 339
Victor Villalobos, Snehal Naik, and David Piwnica-Worms
Chapter 24 Subcellular Localization of Intracellular Human
Proteins by Construction of Tagged Fusion Proteins
and Transient Expression in COS-7 Cells 353
John E. Collins
Chapter 25 GeneFAS: A Tool for the Prediction of Gene Function
Using Multiple Sources of Data 369
Trupti Joshi, Chao Zhang, Guan Ning Lin, Zhao Song,
and Dong Xu
Chapter 26 Comparative Genomics-Based Prediction
of Protein Function 387
Toni Gabaldon
Chapter 27 Design, Manufacture, and Assay of the Efficacy
of siRNAs for Gene Silencing 403
Louise A. Dawson and Badar A. Usmani
Index 421
|
| adam_txt |
Contents
Preface v
Contributors xi
Chapter 1 Whole Genome Amplification with Phi29 DNA
Polymerase to Enable Genetic or Genomic Analysis
of Samples of Low DNA Yield 1
Kaisa Silander and Janna Saarela
Chapter 2 Scanning for DNA Variants by Denaturant Capillary
Electrophoresis 19
Per O. Ekstr0m
Chapter 3 Identification of SNPs, or Mutations
in Sequence Chromatograms 35
Nicole Draper
Chapter 4 BeadArray-Based Genotyping 53
Helen Butler and Jiannis Ragoussis
Chapter 5 Microsatellite-Based Candidate Gene Linkage
Analysis Studies 75
Cathryn Mellersh
Chapter 6 Full Complexity Genomic Hybridization on 60-mer
Oligonucleotide Microarrays for Array Comparative
Genomic Hybridization (aCGH) 87
Alexei Protopopov, Bin Feng, and Lynda Chin
Chapter 7 Detection of Copy Number Changes at Multiple Loci in DNA
Prepared from Formalin-Fixed, Paraffin-Embedded Tissue
by Multiplex Ligation-Dependent Probe Amplification . 101
Minoru Takata
vii
viii Contents
Chapter 8 Application of Microarrays for DNA
Methylation Profiling 109
Axel Schumacher, Andreas Weinhausl, and Arturas Petronis
Chapter 9 Genomewide Identification of Protein Binding
Locations Using Chromatin Immunoprecipitation
Coupled with Microarray 131
Byung-Kwan Cho, Eric M. Knight, and Bernhard 0. Palsson
Chapter 10 Transcriptional Profiling of Small Samples in the
Central Nervous System 147
Stephen D. Ginsberg
Chapter 11 Quantitative Expression Profiling of RNA from
Formalin-Fixed, Paraffin-Embedded Tissues Using
Randomly Assembled Bead Arrays 159
Marina Bibikova, Joanne M. Yeakley,
Jessica Wang-Rodriguez, and Jian-Bing Fan
Chapter 12 Expression Profiling of microRNAs in Cancer Cells:
Technical Considerations 179
Mouldy Sioud and Lina Cekaite
Chapter 13 Identification of Disease Biomarkers by Profiling of Serum
Proteins Using SELDI-TOF Mass Spectrometry 191
Sigrun Langbein
Chapter 14 The Applicability of a Cluster of Differentiation
Monoclonal Antibody Microarray to the
Diagnosis of Human Disease 199
Peter Ellmark, Adrian Woolfson, Larissa Belov, and
Richard I. Christopherson
Chapter 15 Protein Profiling Based on Two-Dimensional
Difference Gel Electrophoresis 211
Gert Van den Bergh and Lutgarde Arckens
Chapter 16 Quantitative Protein Profiling by Mass Spectrometry
Using Isotope-Coded Affinity Tags 225
Arsalan S. Haqqani, John F. Kelly, and Danica B. Stanimirovic
Chapter 17 Quantitative Protein Profiling by Mass Spectrometry
Using Label-Free Proteomics 241
Arsalan S. Haqqani, John F. Kelly, and Danica B. Stanimirovic
I Contents ix
i
j Chapter 18 Using 2D-LC-MS/MS to Identify Francisella tularensis
j Peptides in Extracts from an Infected Mouse
| Macrophage Cell Line 257
j John F. Kelly and Wen Ding
Chapter 19 Baculovirus Expression Vector System:
An Emerging Host for High-Throughput Eukaryotic
Protein Expression 269
Binesh Shrestha, Carol Smee, and Opher Gileadi
Chapter 20 Coimmunoprecipitation and Proteomic Analyses 291
S. Fabio Falsone, Bernd Gesslbauer, and Andreas J. Kungl
Chapter 21 Tandem Affinity Purification Combined with
Mass Spectrometry to Identify Components
of Protein Complexes 309
Peter Kaiser, David Meierhofer, Xiaorong Wang,
and Lan Huang
Chapter 22 Mammalian Two-Hybrid Assay for Detecting
Protein-Protein Interactions in vivo 327
Runtao He and Xuguang Li
Chapter 23 Detection of Protein-Protein Interactions in Live
Cells and Animals with Split Firefly Luciferase
Protein Fragment Complementation 339
Victor Villalobos, Snehal Naik, and David Piwnica-Worms
Chapter 24 Subcellular Localization of Intracellular Human
Proteins by Construction of Tagged Fusion Proteins
and Transient Expression in COS-7 Cells 353
John E. Collins
Chapter 25 GeneFAS: A Tool for the Prediction of Gene Function
Using Multiple Sources of Data 369
Trupti Joshi, Chao Zhang, Guan Ning Lin, Zhao Song,
and Dong Xu
Chapter 26 Comparative Genomics-Based Prediction
of Protein Function 387
Toni Gabaldon
Chapter 27 Design, Manufacture, and Assay of the Efficacy
of siRNAs for Gene Silencing 403
Louise A. Dawson and Badar A. Usmani
Index 421 |
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| spelling | Genomics protocols ed. by Mike Starkey ... 2. ed. Totowa, NJ Humana Press 2008 XV, 425 S. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 439 Chromosome Mapping Laboratory Manuals Gene mapping Laboratory manuals Genomics Laboratory manuals Genomics methods Laboratory Manuals Molecular Biology Laboratory Manuals Sequence Analysis, DNA Laboratory Manuals Genkartierung (DE-588)4123274-4 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Molekulargenetik (DE-588)4039987-4 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Molekulargenetik (DE-588)4039987-4 s Methode (DE-588)4038971-6 s DE-604 Genkartierung (DE-588)4123274-4 s Starkey, Michael P. Sonstige oth Methods in molecular biology 439 (DE-604)BV035362695 439 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016356467&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Genomics protocols Methods in molecular biology Chromosome Mapping Laboratory Manuals Gene mapping Laboratory manuals Genomics Laboratory manuals Genomics methods Laboratory Manuals Molecular Biology Laboratory Manuals Sequence Analysis, DNA Laboratory Manuals Genkartierung (DE-588)4123274-4 gnd Methode (DE-588)4038971-6 gnd Molekulargenetik (DE-588)4039987-4 gnd |
| subject_GND | (DE-588)4123274-4 (DE-588)4038971-6 (DE-588)4039987-4 (DE-588)4143413-4 |
| title | Genomics protocols |
| title_auth | Genomics protocols |
| title_exact_search | Genomics protocols |
| title_exact_search_txtP | Genomics protocols |
| title_full | Genomics protocols ed. by Mike Starkey ... |
| title_fullStr | Genomics protocols ed. by Mike Starkey ... |
| title_full_unstemmed | Genomics protocols ed. by Mike Starkey ... |
| title_short | Genomics protocols |
| title_sort | genomics protocols |
| topic | Chromosome Mapping Laboratory Manuals Gene mapping Laboratory manuals Genomics Laboratory manuals Genomics methods Laboratory Manuals Molecular Biology Laboratory Manuals Sequence Analysis, DNA Laboratory Manuals Genkartierung (DE-588)4123274-4 gnd Methode (DE-588)4038971-6 gnd Molekulargenetik (DE-588)4039987-4 gnd |
| topic_facet | Chromosome Mapping Laboratory Manuals Gene mapping Laboratory manuals Genomics Laboratory manuals Genomics methods Laboratory Manuals Molecular Biology Laboratory Manuals Sequence Analysis, DNA Laboratory Manuals Genkartierung Methode Molekulargenetik Aufsatzsammlung |
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