Oxygen biology and hypoxia:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Amsterdam [u.a.]
Elsevier, Acad. Press
2007
|
| Schriftenreihe: | Methods in enzymology
435 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XLVIII, 543, [18] S. Ill., graph. Darst. |
| ISBN: | 9780123739704 |
Internformat
MARC
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| 245 | 1 | 0 | |a Oxygen biology and hypoxia |c ed. by Helmut Sies... |
| 264 | 1 | |a Amsterdam [u.a.] |b Elsevier, Acad. Press |c 2007 | |
| 300 | |a XLVIII, 543, [18] S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in enzymology |v 435 | |
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| 830 | 0 | |a Methods in enzymology |v 435 |w (DE-604)BV000000938 |9 435 | |
| 856 | 4 | 2 | |m Digitalisierung UB Regensburg |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016250406&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-016250406 | ||
Datensatz im Suchindex
| _version_ | 1804137278151327744 |
|---|---|
| adam_text | Contents
Contributors
xiii
Preface
xxi
Volumes in Series
xxiii
Section I. Hypoxia-lnducible Factor
ι
1. Hypoxia-lnducible Factors PER/ARNT/SIM Domains:
Structure and Function
3
Thomas H.
Scheuermann,
Jinsong Yang, Lei Zhang, Kevin H. Gardner,
and Richard K. Bruick
1.
Introduction
4
2.
Delineation of the HIF PAS Domains
5
3.
Expression and Characterization of HIF PAS Domains
7
4.
Assessing PAS Domain Protein-Protein Interactions
11
5.
Discussion
18
Acknowledgments
20
References
21
2.
Hypoxia-lnducibte Factor Prolyl-Hydroxytase: Purification
and Assays of PHD2
25
Kirsty S. Hewitson, Christopher J. Schofield, and Peter]. Ratcliffe
1.
Introduction
26
2.
Preparation of Purified PHD2 from a Bacterial Source
28
3.
Assaying of PHD2 Activity
29
4.
Indirect Measurements of PHD2 Activity
29
5.
Direct Measurements of PHD2 Hydroxylation Activity
35
6.
Binding Assays
37
7.
Comparison of Assay Formats
38
References
39
vi
Contents
3. Determination and Modulation
of Prolyl-4-Hydroxylase
Domain
Oxygen
Sensor
Activity
43
Renato
Wirthner, Kuppusamy Balamurugan, Daniel
P.
Stiehl, Sandra Barth,
Patrick Spielmann, Felix Oehme, Ingo Flamme, Dörthe M. Katschinski,
Roland H. Wenger, and Gieri Camenisch
1.
Introduction
44
2.
Production of Functionally Active PHDs
48
3.
Determination of PHD by VHL Binding to Peptides Derived from
the HIF-ia ODD Domain
48
4.
Determination of
Prolyl-4-Hydroxylation
by Oxidative
Decarboxylation of 2-Oxoglutarate
51
5.
Crude Tissue Extracts are not a Suitable Source of PHD Activity for the
2-Oxoglutarate Conversion Assay
53
6.
Thin Layer Chromatography to Assess the Purity of [s-^Cfe-Oxoglutarate
53
7.
Application of the 2-Oxoglutarate Conversion Assay to Protein Targets
55
8.
Conclusions
55
Acknowledgments
57
References
57
4.
Characterization of Ankyrin Repeat-Containing Proteins
as Substrates of the Asparaginyl Hydroxylase Factor Inhibiting
Hypoxia-lnducible Transcription Factor
61
Sarah
Linke,
Rachel
).
Hampton-Smith, and Daniel J.
Peet
1.
Introduction
62
2.
Experimental Techniques
65
3.
Discussion/Conclusion
82
Acknowledgments
83
References
83
5.
Transgenic Models to Understand Hypoxia-lnducible
Factor Function
87
Andrew Doedens and Randall S. Johnson
1.
Introduction
88
2.
Hypoxia Response Pathway Genes and Development
90
3.
HIF in Physiology
93
4.
HIF Function in Tumor Biology
98
5.
Summary
100
References
ιοί
Contents
vii
6.
The Silencing Approach of the Hypoxia-Signaling Pathway
107
Edurne
Berra
and Jacques
Pouysségur
1.
A Brief History of RNAi
108
2.
The Hypoxia-Signaling Pathway
109
3.
HIF-a Stability no
4.
HIF Activity
114
5.
HlF-i/HIF-2 Target Gene Specificity
116
6.
RNAi as a New Potential Therapeutic Strategy
116
Acknowledgments
118
References
118
7.
Cellular and Developmental Adaptations to Hypoxia:
A Drosophila
Perspective
123
Nuria Magdalena
Romero,
Andrés
Dekanty, and Pablo
Wappner
1.
Introduction
124
2.
Drosophila
melanogaster as a Model System to Study Physiological
Responses to Hypoxia
124
3.
Experimental Advantages of the Model System
125
4.
The
Drosophila
Respiratory System
126
5.
Occurrence of
a Drosophila
System Homologous to Mammalian HIF
128
6.
Regulation of
Sima
by Oxygen Levels
131
7.
Role of
Sima
and
Fatiga
in
Drosophila
Development
132
8.
Hypoxia-lnducible Genes and the Adaptation of
Drosophila
to
Oxygen Starvation
134
9.
Regulation of
Sima
by the PI3K and TOR Pathways
134
10.
Role of the HIF System in Growth Control and Cell Size Determination
136
11.
Concluding Remarks
138
Acknowledgments
139
References
139
Section II. Erythropoietin
145
8.
Constitutively Overexpressed Erythropoietin Reduces
Infarct
Size
in a Mouse Model of Permanent Coronary Artery Ligation
147
Giovanni G.
Camici,
Thomas
Stallmach,
Matthias Hermann,
Rutger Hassink,
Peter Doevendans, Beat Grenadier, Alain Hirschy,
Jobannes Vogel,
Thomas
F. Lüscher,
Frank Ruschitzka, and Max
Gassmann
1.
Introduction
148
2.
Material and Methods
149
3.
Results
151
4.
Discussion
153
viii Contents
Acknowledgments
154
References
154
9.
Use of Gene-Manipulated Mice in the Study of
Erythropoietin
Gene Expression
157
Norio
Suzuki, Naoshi
Obara,
and Masayuki Yamamoto
1.
Introduction
158
2.
Materials
161
3.
Methods and Results
163
4.
Conclusion
173
Acknowledgments
173
References
174
10.
Control of Erythropoietin Gene Expression and its
Use in Medicine
179
Wolfgang Jelkmann
1.
Introduction
180
2.
Native
ЕРО
Gene Expression and its
Pharmacologie
Stimulation
г8о
3.
ЕРО
Gene Transfer
184
4.
Recombinant
EPOS
187
5.
Conclusions
190
References
191
11.
Role of Hypoxia-lnducible Factor-act in Endothelial
Development and
Hematopoyesis
199
Osamu
Ohneda, Masumi Nagano, and Yoshiaki Fujii-Kuriyama
1.
Introduction
200
2.
Vasculogenesis/Angiogenesis and HlFs
200
3.
HIF-1/3/ARNT Null Mice
204
4.
Neovascularization and HIFs
205
5.
Hematopoiesis and HIFs
211
6.
Conclusion
214
References
214
Section III. Hypoxia and Adaptation
219
12.
Organ Protection by Hypoxia and Hypoxia-lnducible Factors
221
Wanja M.
Bernhardt,
Christina
Warnecke, Carsten
WiUam, Tetsuhiro Tanaka,
Michael S. Wiesener, and Kai-Uwe Eckardt
1.
Introduction
222
2.
From
Ischemie
to
Hypoxie
Preconditioning
222
Contents
¡χ
3. Hypoxia-Inducible
Transcription Factors
223
4.
Strategies to Activate HIF and HIF Target Genes
227
5. Hypoxie
Preconditioning and HIF
231
6.
HIF in Chronic Hypoxic/lschemic Diseases
236
7.
Conclusions and Perspectives
237
References
238
13.
Hypoxia and Regulation of Messenger
RNA
Translation
247
Marianne Koritzinsky and
Bradly
G.
Wouters
1.
Introduction
248
2.
Changes in Global mRNA Translation During Hypoxia
249
3.
Molecular Mechanisms that Regulate mRNA Translation During Hypoxia
251
4.
Methods Employed to Study mRNA Translation During Hypoxia
256
5.
Protocols
268
References
271
14.
Hypoxia and the Unfolded Protein Response
275
Constantinos Koumenis, Meixia Bi, Jiangbin Ye, Douglas Feldman,
and Albert
С
Koong
1.
Introduction
276
2.
Methods Employed in Detecting
Hypoxie
Induction of
ER
Stress
282
Acknowledgments
289
References
290
Section IV. Hypoxia and Tumor Biology
295
15.
Tumor Hypoxia in Cancer Therapy
297
J. Martin Brown
1.
Hypoxia in Human Tumors
298
2.
The Dynamic Nature of Hypoxia in Tumors
300
3.
Consequences of Tumor Hypoxia for Cancer Treatment
300
4.
Size of the Oxygen Effect with Radiation
302
5.
The Influence of Tumor Hypoxia on Cancer Treatment
by Radiotherapy
303
6.
Influence of Tumor Hypoxia on Response to Chemotherapy
307
7.
Exploiting Hypoxia in Cancer Treatment
308
References
315
16.
HIF Gene Expression
ín
Cancer Therapy
323
Denise
A. Chan, Adam j.
Krieg,
Sandra
Turcotte,
and
Amato J. Giaccia
1.
Introduction
324
2,
Experimental Procedures
326
χ
Contents
3.
Conclusions
337
Acknowledgments
337
References
338
17.
Analysis of Hypoxia-lnducible Factor-ia Expression and its
Effects on Invasion and Metastasis
347
Balaji Krishnamachary and Gregg L.
Semenza
α.
Introduction
347
2.
Protocol
1:
HIF-ia Immunohistochemistry
349
3.
Protocol
2:
Invasion Assay
350
4.
Protocol
3:
Transepithelial Resistance Measurement of
Cell-Cell Adhesion
351
5.
Protocol
4:
Analysis of mRNA Expression by qRT-PCR 35a
References
352
18.
Macrophage
Migration Inhibitory Factor Manipulation and
Evaluation in
Tumoral
Hypoxie
Adaptation
355
Millicent Winner, Lin
Leng,
Wayne Zundel, and Robert A. Mitchell
1.
Introduction
356
2.
Modulation of MIF Levels by Targeted shRNAs and Assessment of
Knockdown Efficiency
357
3.
Analysis of MIF-Dependent CSN5 and COP9 Signaiosome Function
362
4.
Determination of Tumor-Associated MIF Expression and MIF
Polymorphic Disparity
364
5.
Conclusions
367
References
367
19.
The
Von Hippel-Lindau
Tumor Suppressor Protein: An Update
371
William G. Kaelin, jr.
1.
Introduction
372
2.
Regulation of Epithelial Differentiation by pVHL
373
3.
Crosstalk Between
с
-Met
and VHL
374
4.
Regulation of
Neuronal Apoptosis
by pVHL
375
5.
Possible Links Between P53 and pVHL
376
6.
Regulation of pVHL by Phosphorylation
376
7.
Polyubiquitylation of pVHL
377
8.
Mouse Models for Studying pVHL Function
377
References
378
Contents xi
20.
Hypoxia-lnducible Factor
ι
Inhibitors
385
Giovanni
Melillo
1.
Introduction
386
2.
Cell-Based High Throughput Screens
387
3.
Cell-Free Assays
393
4.
Bioassay-Directed Isolation of Natural Product HIF-i Inhibitors
398
5.
Conclusions
399
Acknowledgments
399
References
400
Section V. Hypoxia and Inflammatory Mediators
403
21.
Regulation of Hypoxia-lnducible Factors During Inflammation
405
Stilla
Frede,
Utta Berchner-Pfannschmidt,
and Joachim Fandrey
1.
Introduction
406
2.
Regulation of HIF at the Transcriptional Level
408
3.
Regulation of HIF at the Translational Level
410
4.
Regulation of HIF-ia at the Posttranslational Level
411
5.
Regulation of HIF-i Activity
413
6.
Perspectives
414
7.
Conclusions
414
Acknowledgments
415
References
415
22. Superoxide
and Derived Reactive Oxygen Species in the
Regulation of Hypoxia-lnducible Factors
421
Agnes
Görlach
and Thomas Kietzmann
1.
Introduction
422
2.
Reactive Oxygen Species Act as Signaling Molecules
423
3.
HlFs are Sensitive to Oxygen
424
4.
Reactive Oxygen Species Modulate HIF
425
5.
How are HIFs Regulated by Reactive Oxygen Species?
427
6.
Summary
431
7.
Methods
431
8.
The Cytochrome
С
Reduction Assay for Detection of Extracellular
Reactive Oxygen Species
431
9.
Chemiluminescence Assay for Detection of ExtraceUular Reactive
Oxygen Species
432
io.
Measuring Intracellular Production of Reactive Oxygen Species using
Fiuorescent Dyes
433
xii
Contents
її.
Detection of Reactive Oxygen Species by Electron Paramagnetic
Resonance
436
Acknowledgments
438
References
438
23.
Genetics of Mitochondrial Electron Transport Chain in
Regulating Oxygen Sensing
447
Eric L. Belt and Navdeep S. Chandel
1.
Introduction
448
2.
Detecting HIF-ia Protein Levels
449
3.
Detecting Intracellular
ROS
Levels
452
4.
Method
1:
Examining
Hypoxie
Stabilization of HIF-ia Protein in Cells
Containing RNAI against the Rieske Fe-S Protein
454
5.
Method
2:
Examining the Role of
ROS
Generated from Mitochondrial
Electron Transport in
Hypoxie
Stabilization of
H
IF-
ία
Protein
458
6.
Concluding Remarks
459
Acknowledgments
459
References
460
24.
Hypoxia-lnducible Factor-i« Under the Control
of Nitric Oxide
463
Bernhard
Brune
and Jie Zhou
1.
HIF-i and Oxygen Sensing
464
2.
Nitric Oxide: A Multifunctional Messenger
465
3.
Accumulation of HIF-ia and Activation of HIF-i by NO
467
4. Superoxide
Stabilizes HIF-iot but Antagonizes NO Actions
470
5. Hypoxie
Signal Transmission is Antagonized by NO
472
6.
Summary and Conclusions
473
Acknowledgments
475
References
475
25. Hypoxie
Regulation of
NF
-л
В
Signaling
479
Eoin P. Cummins, Katrina M. Comerford,
Carsten Scholz, Ulrike Bruning,
and Cormac T. Taylor
1.
Background
480
2.
Treatment Protocols for Cellular Hypoxia Studies
481
3.
Measurement of NF-kB Activity in Cultured Cells
484
4.
Summary/Conclusions
491
References
491
Author index
493
Subject Index
535
|
| adam_txt |
Contents
Contributors
xiii
Preface
xxi
Volumes in Series
xxiii
Section I. Hypoxia-lnducible Factor
ι
1. Hypoxia-lnducible Factors PER/ARNT/SIM Domains:
Structure and Function
3
Thomas H.
Scheuermann,
Jinsong Yang, Lei Zhang, Kevin H. Gardner,
and Richard K. Bruick
1.
Introduction
4
2.
Delineation of the HIF PAS Domains
5
3.
Expression and Characterization of HIF PAS Domains
7
4.
Assessing PAS Domain Protein-Protein Interactions
11
5.
Discussion
18
Acknowledgments
20
References
21
2.
Hypoxia-lnducibte Factor Prolyl-Hydroxytase: Purification
and Assays of PHD2
25
Kirsty S. Hewitson, Christopher J. Schofield, and Peter]. Ratcliffe
1.
Introduction
26
2.
Preparation of Purified PHD2 from a Bacterial Source
28
3.
Assaying of PHD2 Activity
29
4.
Indirect Measurements of PHD2 Activity
29
5.
Direct Measurements of PHD2 Hydroxylation Activity
35
6.
Binding Assays
37
7.
Comparison of Assay Formats
38
References
39
vi
Contents
3. Determination and Modulation
of Prolyl-4-Hydroxylase
Domain
Oxygen
Sensor
Activity
43
Renato
Wirthner, Kuppusamy Balamurugan, Daniel
P.
Stiehl, Sandra Barth,
Patrick Spielmann, Felix Oehme, Ingo Flamme, Dörthe M. Katschinski,
Roland H. Wenger, and Gieri Camenisch
1.
Introduction
44
2.
Production of Functionally Active PHDs
48
3.
Determination of PHD by VHL Binding to Peptides Derived from
the HIF-ia ODD Domain
48
4.
Determination of
Prolyl-4-Hydroxylation
by Oxidative
Decarboxylation of 2-Oxoglutarate
51
5.
Crude Tissue Extracts are not a Suitable Source of PHD Activity for the
2-Oxoglutarate Conversion Assay
53
6.
Thin Layer Chromatography to Assess the Purity of [s-^Cfe-Oxoglutarate
53
7.
Application of the 2-Oxoglutarate Conversion Assay to Protein Targets
55
8.
Conclusions
55
Acknowledgments
57
References
57
4.
Characterization of Ankyrin Repeat-Containing Proteins
as Substrates of the Asparaginyl Hydroxylase Factor Inhibiting
Hypoxia-lnducible Transcription Factor
61
Sarah
Linke,
Rachel
).
Hampton-Smith, and Daniel J.
Peet
1.
Introduction
62
2.
Experimental Techniques
65
3.
Discussion/Conclusion
82
Acknowledgments
83
References
83
5.
Transgenic Models to Understand Hypoxia-lnducible
Factor Function
87
Andrew Doedens and Randall S. Johnson
1.
Introduction
88
2.
Hypoxia Response Pathway Genes and Development
90
3.
HIF in Physiology
93
4.
HIF Function in Tumor Biology
98
5.
Summary
100
References
ιοί
Contents
vii
6.
The Silencing Approach of the Hypoxia-Signaling Pathway
107
Edurne
Berra
and Jacques
Pouysségur
1.
A Brief History of RNAi
108
2.
The Hypoxia-Signaling Pathway
109
3.
HIF-a Stability no
4.
HIF Activity
114
5.
HlF-i/HIF-2 Target Gene Specificity
116
6.
RNAi as a New Potential Therapeutic Strategy
116
Acknowledgments
118
References
118
7.
Cellular and Developmental Adaptations to Hypoxia:
A Drosophila
Perspective
123
Nuria Magdalena
Romero,
Andrés
Dekanty, and Pablo
Wappner
1.
Introduction
124
2.
Drosophila
melanogaster as a Model System to Study Physiological
Responses to Hypoxia
124
3.
Experimental Advantages of the Model System
125
4.
The
Drosophila
Respiratory System
126
5.
Occurrence of
a Drosophila
System Homologous to Mammalian HIF
128
6.
Regulation of
Sima
by Oxygen Levels
131
7.
Role of
Sima
and
Fatiga
in
Drosophila
Development
132
8.
Hypoxia-lnducible Genes and the Adaptation of
Drosophila
to
Oxygen Starvation
134
9.
Regulation of
Sima
by the PI3K and TOR Pathways
134
10.
Role of the HIF System in Growth Control and Cell Size Determination
136
11.
Concluding Remarks
138
Acknowledgments
139
References
139
Section II. Erythropoietin
145
8.
Constitutively Overexpressed Erythropoietin Reduces
Infarct
Size
in a Mouse Model of Permanent Coronary Artery Ligation
147
Giovanni G.
Camici,
Thomas
Stallmach,
Matthias Hermann,
Rutger Hassink,
Peter Doevendans, Beat Grenadier, Alain Hirschy,
Jobannes Vogel,
Thomas
F. Lüscher,
Frank Ruschitzka, and Max
Gassmann
1.
Introduction
148
2.
Material and Methods
149
3.
Results
151
4.
Discussion
153
viii Contents
Acknowledgments
154
References
154
9.
Use of Gene-Manipulated Mice in the Study of
Erythropoietin
Gene Expression
157
Norio
Suzuki, Naoshi
Obara,
and Masayuki Yamamoto
1.
Introduction
158
2.
Materials
161
3.
Methods and Results
163
4.
Conclusion
173
Acknowledgments
173
References
174
10.
Control of Erythropoietin Gene Expression and its
Use in Medicine
179
Wolfgang Jelkmann
1.
Introduction
180
2.
Native
ЕРО
Gene Expression and its
Pharmacologie
Stimulation
г8о
3.
ЕРО
Gene Transfer
184
4.
Recombinant
EPOS
187
5.
Conclusions
190
References
191
11.
Role of Hypoxia-lnducible Factor-act in Endothelial
Development and
Hematopoyesis
199
Osamu
Ohneda, Masumi Nagano, and Yoshiaki Fujii-Kuriyama
1.
Introduction
200
2.
Vasculogenesis/Angiogenesis and HlFs
200
3.
HIF-1/3/ARNT Null Mice
204
4.
Neovascularization and HIFs
205
5.
Hematopoiesis and HIFs
211
6.
Conclusion
214
References
214
Section III. Hypoxia and Adaptation
219
12.
Organ Protection by Hypoxia and Hypoxia-lnducible Factors
221
Wanja M.
Bernhardt,
Christina
Warnecke, Carsten
WiUam, Tetsuhiro Tanaka,
Michael S. Wiesener, and Kai-Uwe Eckardt
1.
Introduction
222
2.
From
Ischemie
to
Hypoxie
Preconditioning
222
Contents
¡χ
3. Hypoxia-Inducible
Transcription Factors
223
4.
Strategies to Activate HIF and HIF Target Genes
227
5. Hypoxie
Preconditioning and HIF
231
6.
HIF in Chronic Hypoxic/lschemic Diseases
236
7.
Conclusions and Perspectives
237
References
238
13.
Hypoxia and Regulation of Messenger
RNA
Translation
247
Marianne Koritzinsky and
Bradly
G.
Wouters
1.
Introduction
248
2.
Changes in Global mRNA Translation During Hypoxia
249
3.
Molecular Mechanisms that Regulate mRNA Translation During Hypoxia
251
4.
Methods Employed to Study mRNA Translation During Hypoxia
256
5.
Protocols
268
References
271
14.
Hypoxia and the Unfolded Protein Response
275
Constantinos Koumenis, Meixia Bi, Jiangbin Ye, Douglas Feldman,
and Albert
С
Koong
1.
Introduction
276
2.
Methods Employed in Detecting
Hypoxie
Induction of
ER
Stress
282
Acknowledgments
289
References
290
Section IV. Hypoxia and Tumor Biology
295
15.
Tumor Hypoxia in Cancer Therapy
297
J. Martin Brown
1.
Hypoxia in Human Tumors
298
2.
The Dynamic Nature of Hypoxia in Tumors
300
3.
Consequences of Tumor Hypoxia for Cancer Treatment
300
4.
Size of the Oxygen Effect with Radiation
302
5.
The Influence of Tumor Hypoxia on Cancer Treatment
by Radiotherapy
303
6.
Influence of Tumor Hypoxia on Response to Chemotherapy
307
7.
Exploiting Hypoxia in Cancer Treatment
308
References
315
16.
HIF Gene Expression
ín
Cancer Therapy
323
Denise
A. Chan, Adam j.
Krieg,
Sandra
Turcotte,
and
Amato J. Giaccia
1.
Introduction
324
2,
Experimental Procedures
326
χ
Contents
3.
Conclusions
337
Acknowledgments
337
References
338
17.
Analysis of Hypoxia-lnducible Factor-ia Expression and its
Effects on Invasion and Metastasis
347
Balaji Krishnamachary and Gregg L.
Semenza
α.
Introduction
347
2.
Protocol
1:
HIF-ia Immunohistochemistry
349
3.
Protocol
2:
Invasion Assay
350
4.
Protocol
3:
Transepithelial Resistance Measurement of
Cell-Cell Adhesion
351
5.
Protocol
4:
Analysis of mRNA Expression by qRT-PCR 35a
References
352
18.
Macrophage
Migration Inhibitory Factor Manipulation and
Evaluation in
Tumoral
Hypoxie
Adaptation
355
Millicent Winner, Lin
Leng,
Wayne Zundel, and Robert A. Mitchell
1.
Introduction
356
2.
Modulation of MIF Levels by Targeted shRNAs and Assessment of
Knockdown Efficiency
357
3.
Analysis of MIF-Dependent CSN5 and COP9 Signaiosome Function
362
4.
Determination of Tumor-Associated MIF Expression and MIF
Polymorphic Disparity
364
5.
Conclusions
367
References
367
19.
The
Von Hippel-Lindau
Tumor Suppressor Protein: An Update
371
William G. Kaelin, jr.
1.
Introduction
372
2.
Regulation of Epithelial Differentiation by pVHL
373
3.
Crosstalk Between
с
-Met
and VHL
374
4.
Regulation of
Neuronal Apoptosis
by pVHL
375
5.
Possible Links Between P53 and pVHL
376
6.
Regulation of pVHL by Phosphorylation
376
7.
Polyubiquitylation of pVHL
377
8.
Mouse Models for Studying pVHL Function
377
References
378
Contents xi
20.
Hypoxia-lnducible Factor
ι
Inhibitors
385
Giovanni
Melillo
1.
Introduction
386
2.
Cell-Based High Throughput Screens
387
3.
Cell-Free Assays
393
4.
Bioassay-Directed Isolation of Natural Product HIF-i Inhibitors
398
5.
Conclusions
399
Acknowledgments
399
References
400
Section V. Hypoxia and Inflammatory Mediators
403
21.
Regulation of Hypoxia-lnducible Factors During Inflammation
405
Stilla
Frede,
Utta Berchner-Pfannschmidt,
and Joachim Fandrey
1.
Introduction
406
2.
Regulation of HIF at the Transcriptional Level
408
3.
Regulation of HIF at the Translational Level
410
4.
Regulation of HIF-ia at the Posttranslational Level
411
5.
Regulation of HIF-i Activity
413
6.
Perspectives
414
7.
Conclusions
414
Acknowledgments
415
References
415
22. Superoxide
and Derived Reactive Oxygen Species in the
Regulation of Hypoxia-lnducible Factors
421
Agnes
Görlach
and Thomas Kietzmann
1.
Introduction
422
2.
Reactive Oxygen Species Act as Signaling Molecules
423
3.
HlFs are Sensitive to Oxygen
424
4.
Reactive Oxygen Species Modulate HIF
425
5.
How are HIFs Regulated by Reactive Oxygen Species?
427
6.
Summary
431
7.
Methods
431
8.
The Cytochrome
С
Reduction Assay for Detection of Extracellular
Reactive Oxygen Species
431
9.
Chemiluminescence Assay for Detection of ExtraceUular Reactive
Oxygen Species
432
io.
Measuring Intracellular Production of Reactive Oxygen Species using
Fiuorescent Dyes
433
xii
Contents
її.
Detection of Reactive Oxygen Species by Electron Paramagnetic
Resonance
436
Acknowledgments
438
References
438
23.
Genetics of Mitochondrial Electron Transport Chain in
Regulating Oxygen Sensing
447
Eric L. Belt and Navdeep S. Chandel
1.
Introduction
448
2.
Detecting HIF-ia Protein Levels
449
3.
Detecting Intracellular
ROS
Levels
452
4.
Method
1:
Examining
Hypoxie
Stabilization of HIF-ia Protein in Cells
Containing RNAI against the Rieske Fe-S Protein
454
5.
Method
2:
Examining the Role of
ROS
Generated from Mitochondrial
Electron Transport in
Hypoxie
Stabilization of
H
IF-
ία
Protein
458
6.
Concluding Remarks
459
Acknowledgments
459
References
460
24.
Hypoxia-lnducible Factor-i« Under the Control
of Nitric Oxide
463
Bernhard
Brune
and Jie Zhou
1.
HIF-i and Oxygen Sensing
464
2.
Nitric Oxide: A Multifunctional Messenger
465
3.
Accumulation of HIF-ia and Activation of HIF-i by NO
467
4. Superoxide
Stabilizes HIF-iot but Antagonizes NO Actions
470
5. Hypoxie
Signal Transmission is Antagonized by NO
472
6.
Summary and Conclusions
473
Acknowledgments
475
References
475
25. Hypoxie
Regulation of
NF
-л
В
Signaling
479
Eoin P. Cummins, Katrina M. Comerford,
Carsten Scholz, Ulrike Bruning,
and Cormac T. Taylor
1.
Background
480
2.
Treatment Protocols for Cellular Hypoxia Studies
481
3.
Measurement of NF-kB Activity in Cultured Cells
484
4.
Summary/Conclusions
491
References
491
Author index
493
Subject Index
535 |
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| genre_facet | Aufsatzsammlung Statistik |
| id | DE-604.BV023046963 |
| illustrated | Illustrated |
| index_date | 2024-07-02T19:22:59Z |
| indexdate | 2024-07-09T21:09:45Z |
| institution | BVB |
| isbn | 9780123739704 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-016250406 |
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| physical | XLVIII, 543, [18] S. Ill., graph. Darst. |
| publishDate | 2007 |
| publishDateSearch | 2007 |
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| publisher | Elsevier, Acad. Press |
| record_format | marc |
| series | Methods in enzymology |
| series2 | Methods in enzymology |
| spelling | Oxygen biology and hypoxia ed. by Helmut Sies... Amsterdam [u.a.] Elsevier, Acad. Press 2007 XLVIII, 543, [18] S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in enzymology 435 Labortechnik (DE-588)4123602-6 gnd rswk-swf Molekularbiologie (DE-588)4039983-7 gnd rswk-swf Hypoxie (DE-588)4133549-1 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content (DE-588)4056995-0 Statistik gnd-content Hypoxie (DE-588)4133549-1 s Molekularbiologie (DE-588)4039983-7 s Labortechnik (DE-588)4123602-6 s DE-604 Sies, Helmut Sonstige oth Methods in enzymology 435 (DE-604)BV000000938 435 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016250406&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Oxygen biology and hypoxia Methods in enzymology Labortechnik (DE-588)4123602-6 gnd Molekularbiologie (DE-588)4039983-7 gnd Hypoxie (DE-588)4133549-1 gnd |
| subject_GND | (DE-588)4123602-6 (DE-588)4039983-7 (DE-588)4133549-1 (DE-588)4143413-4 (DE-588)4056995-0 |
| title | Oxygen biology and hypoxia |
| title_auth | Oxygen biology and hypoxia |
| title_exact_search | Oxygen biology and hypoxia |
| title_exact_search_txtP | Oxygen biology and hypoxia |
| title_full | Oxygen biology and hypoxia ed. by Helmut Sies... |
| title_fullStr | Oxygen biology and hypoxia ed. by Helmut Sies... |
| title_full_unstemmed | Oxygen biology and hypoxia ed. by Helmut Sies... |
| title_short | Oxygen biology and hypoxia |
| title_sort | oxygen biology and hypoxia |
| topic | Labortechnik (DE-588)4123602-6 gnd Molekularbiologie (DE-588)4039983-7 gnd Hypoxie (DE-588)4133549-1 gnd |
| topic_facet | Labortechnik Molekularbiologie Hypoxie Aufsatzsammlung Statistik |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016250406&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV000000938 |
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