PCR primer design:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, N.J.
Humana Press
2007
|
| Schriftenreihe: | Methods in molecular biology (Clifton, N.J.)
402 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Includes bibliographical references and index |
| Beschreibung: | XIII, 431 S. Ill., graph. Darst. 24 cm |
| ISBN: | 9781588297259 158829725X |
Internformat
MARC
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| 245 | 1 | 0 | |a PCR primer design |c edited by Anton Yuryev |
| 264 | 1 | |a Totowa, N.J. |b Humana Press |c 2007 | |
| 300 | |a XIII, 431 S. |b Ill., graph. Darst. |c 24 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in molecular biology (Clifton, N.J.) |v 402 | |
| 500 | |a Includes bibliographical references and index | ||
| 650 | 2 | |a DNA-directed DNA polymerase | |
| 650 | 7 | |a Réaction en chaîne de la polymérase |2 ram | |
| 650 | 2 | |a Réaction polymérisation en chaîne | |
| 650 | 4 | |a Polymerase chain reaction | |
| 650 | 4 | |a DNA polymerases | |
| 650 | 0 | 7 | |a Polymerase-Kettenreaktion |0 (DE-588)4256726-9 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Primer |g Molekulargenetik |0 (DE-588)4318719-5 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4143413-4 |a Aufsatzsammlung |2 gnd-content | |
| 689 | 0 | 0 | |a Polymerase-Kettenreaktion |0 (DE-588)4256726-9 |D s |
| 689 | 0 | 1 | |a Primer |g Molekulargenetik |0 (DE-588)4318719-5 |D s |
| 689 | 0 | |C b |5 DE-604 | |
| 700 | 1 | |a Yuryev, Anton |e Sonstige |4 oth | |
| 830 | 0 | |a Methods in molecular biology (Clifton, N.J.) |v 402 |w (DE-604)BV035362695 |9 402 | |
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| 999 | |a oai:aleph.bib-bvb.de:BVB01-016224922 | ||
Datensatz im Suchindex
| _version_ | 1804137240507449344 |
|---|---|
| adam_text | Contents
Preface v
Contributors xi
Part I: Basic Principles and Software for PCR
Primer Design
1. Physical Principles and Visual OMP Software
for Optimal PCR Design
John SantaLucia, Jr 3
2. OLIGO 7 Primer Analysis Software
Wojciech Rychlik 35
3. Selection for 3 End Triplets for Polymerase
Chain Reaction Primers
Kenji Onodera 61
4. The Reference Point Method in Primer Design
Thomas Kampke 75
5. PCR Primer Design Using Statistical Modeling
Anton Yuryev 93
6. Developing a Statistical Model for Primer Design
Jianping Huang and Anton Yuryev 105
Part II: Genome Scale PCR Primer Design
7. GST PRIME: An Algorithm for Genome Wide Primer Design
Dario Leister and Claudio Varotto 141
8. Genome Scale Probe and Primer Design
with PR1MEGENS
Gyan Prakash Srivastava and Dong Xu 159
A. Repeat Masking for PCR Primer Design
9. SNPbox: Web Based High Throughput Primer Design
with an Eye for Repetitive Sequences
Stefan Weckx, Peter De Rijk, Wim Glassee, Christine Van
Broeckhoven, and Jurgen Del Favero 179
vii
viii Contents
10. Fast Masking of Repeated Primer Binding Sites in Eukaryotic
Genomes
Reidar Andreson, Lauris Kaplinski, and Maido Remm 201
B. Multiplex PCR Primer Design
11. Degenerate Primer Design: Theoretical Analysis and the HYDEN
Program
Chaim Linhart and Ron Shamir 221
12. An Iterative Method for Selecting Degenerate Multiplex PCR
Primers
Richard Souvenir, Jeremy Buhler, Gary Stormo, and Weixiong
Zhang 245
13. Primer Design for Multiplexed Genotyping
Lars Kaderali 269
14. MultiPLX: Automatic Grouping and Evaluation
of PCR Primers
Lauris Kaplinski and Maido Remm 287
15. MultiPrimer: A System for Microarray PCR
Primer Design
Rohan Fernandes and Steven Skiena 305
C. Allele specific PCR
16. Modified Oligonucleotides as Tools for Allele Specific
Amplification
Michael Strerath, Ilka Detmer, Jens Gaster,
and Andreas Marx 317
17. AllelelD: A Pathogen Detection and Identification System
Arun Apte and Siddharth Singh 329
D. Long PCR Primer Design
18. Designing Primers for Whole Genome PCR Scanning
Using the Software Package GenoFrag: A Software Package
for the Design of Primers Dedicated to Whole Genome
Scanning by LR PCR
Nouri Ben Zakour and Yves Le Loir 349
E. DNA Methylation Mapping
19. Designing PCR Primer for DNA Methylation Mapping
Long Cheng Li 371
Contents ix
20. BiSearch: ePCR Tool for Native or Bisulfite Treated Genomic
Template
Tamas Aranyi and Gabor E. Tusnady 385
21. Graphical Design of Primers with PerlPrimer
Owen Marshall 403
Index 475
|
| adam_txt |
Contents
Preface v
Contributors xi
Part I: Basic Principles and Software for PCR
Primer Design
1. Physical Principles and Visual OMP Software
for Optimal PCR Design
John SantaLucia, Jr 3
2. OLIGO 7 Primer Analysis Software
Wojciech Rychlik 35
3. Selection for 3' End Triplets for Polymerase
Chain Reaction Primers
Kenji Onodera 61
4. The Reference Point Method in Primer Design
Thomas Kampke 75
5. PCR Primer Design Using Statistical Modeling
Anton Yuryev 93
6. Developing a Statistical Model for Primer Design
Jianping Huang and Anton Yuryev 105
Part II: Genome Scale PCR Primer Design
7. GST PRIME: An Algorithm for Genome Wide Primer Design
Dario Leister and Claudio Varotto 141
8. Genome Scale Probe and Primer Design
with PR1MEGENS
Gyan Prakash Srivastava and Dong Xu 159
A. Repeat Masking for PCR Primer Design
9. SNPbox: Web Based High Throughput Primer Design
with an Eye for Repetitive Sequences
Stefan Weckx, Peter De Rijk, Wim Glassee, Christine Van
Broeckhoven, and Jurgen Del Favero 179
vii
viii Contents
10. Fast Masking of Repeated Primer Binding Sites in Eukaryotic
Genomes
Reidar Andreson, Lauris Kaplinski, and Maido Remm 201
B. Multiplex PCR Primer Design
11. Degenerate Primer Design: Theoretical Analysis and the HYDEN
Program
Chaim Linhart and Ron Shamir 221
12. An Iterative Method for Selecting Degenerate Multiplex PCR
Primers
Richard Souvenir, Jeremy Buhler, Gary Stormo, and Weixiong
Zhang 245
13. Primer Design for Multiplexed Genotyping
Lars Kaderali 269
14. MultiPLX: Automatic Grouping and Evaluation
of PCR Primers
Lauris Kaplinski and Maido Remm 287
15. MultiPrimer: A System for Microarray PCR
Primer Design
Rohan Fernandes and Steven Skiena 305
C. Allele specific PCR
16. Modified Oligonucleotides as Tools for Allele Specific
Amplification
Michael Strerath, Ilka Detmer, Jens Gaster,
and Andreas Marx 317
17. AllelelD: A Pathogen Detection and Identification System
Arun Apte and Siddharth Singh 329
D. Long PCR Primer Design
18. Designing Primers for Whole Genome PCR Scanning
Using the Software Package GenoFrag: A Software Package
for the Design of Primers Dedicated to Whole Genome
Scanning by LR PCR
Nouri Ben Zakour and Yves Le Loir 349
E. DNA Methylation Mapping
19. Designing PCR Primer for DNA Methylation Mapping
Long Cheng Li 371
Contents ix
20. BiSearch: ePCR Tool for Native or Bisulfite Treated Genomic
Template
Tamas Aranyi and Gabor E. Tusnady 385
21. Graphical Design of Primers with PerlPrimer
Owen Marshall 403
Index 475 |
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| illustrated | Illustrated |
| index_date | 2024-07-02T19:13:05Z |
| indexdate | 2024-07-09T21:09:09Z |
| institution | BVB |
| isbn | 9781588297259 158829725X |
| language | English |
| lccn | 2007925517 |
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| physical | XIII, 431 S. Ill., graph. Darst. 24 cm |
| publishDate | 2007 |
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| publisher | Humana Press |
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| series | Methods in molecular biology (Clifton, N.J.) |
| series2 | Methods in molecular biology (Clifton, N.J.) |
| spelling | PCR primer design edited by Anton Yuryev Totowa, N.J. Humana Press 2007 XIII, 431 S. Ill., graph. Darst. 24 cm txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology (Clifton, N.J.) 402 Includes bibliographical references and index DNA-directed DNA polymerase Réaction en chaîne de la polymérase ram Réaction polymérisation en chaîne Polymerase chain reaction DNA polymerases Polymerase-Kettenreaktion (DE-588)4256726-9 gnd rswk-swf Primer Molekulargenetik (DE-588)4318719-5 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Polymerase-Kettenreaktion (DE-588)4256726-9 s Primer Molekulargenetik (DE-588)4318719-5 s b DE-604 Yuryev, Anton Sonstige oth Methods in molecular biology (Clifton, N.J.) 402 (DE-604)BV035362695 402 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016224922&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | PCR primer design Methods in molecular biology (Clifton, N.J.) DNA-directed DNA polymerase Réaction en chaîne de la polymérase ram Réaction polymérisation en chaîne Polymerase chain reaction DNA polymerases Polymerase-Kettenreaktion (DE-588)4256726-9 gnd Primer Molekulargenetik (DE-588)4318719-5 gnd |
| subject_GND | (DE-588)4256726-9 (DE-588)4318719-5 (DE-588)4143413-4 |
| title | PCR primer design |
| title_auth | PCR primer design |
| title_exact_search | PCR primer design |
| title_exact_search_txtP | PCR primer design |
| title_full | PCR primer design edited by Anton Yuryev |
| title_fullStr | PCR primer design edited by Anton Yuryev |
| title_full_unstemmed | PCR primer design edited by Anton Yuryev |
| title_short | PCR primer design |
| title_sort | pcr primer design |
| topic | DNA-directed DNA polymerase Réaction en chaîne de la polymérase ram Réaction polymérisation en chaîne Polymerase chain reaction DNA polymerases Polymerase-Kettenreaktion (DE-588)4256726-9 gnd Primer Molekulargenetik (DE-588)4318719-5 gnd |
| topic_facet | DNA-directed DNA polymerase Réaction en chaîne de la polymérase Réaction polymérisation en chaîne Polymerase chain reaction DNA polymerases Polymerase-Kettenreaktion Primer Molekulargenetik Aufsatzsammlung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016224922&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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