Integrins:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Amsterdam [u.a.]
Elsevier, Acad. Press
2007
|
| Schriftenreihe: | Methods in enzymology
426 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XLV, 567, [12] Bl. Ill., graph. Darst. |
| ISBN: | 9780123739247 |
Internformat
MARC
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| 020 | |a 9780123739247 |9 978-0-12-373924-7 | ||
| 035 | |a (OCoLC)173498265 | ||
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| 084 | |a MED 308f |2 stub | ||
| 084 | |a CHE 820f |2 stub | ||
| 245 | 1 | 0 | |a Integrins |c ed. by David A. Cheresh |
| 264 | 1 | |a Amsterdam [u.a.] |b Elsevier, Acad. Press |c 2007 | |
| 300 | |a XLV, 567, [12] Bl. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in enzymology |v 426 | |
| 650 | 4 | |a Integrins | |
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| 830 | 0 | |a Methods in enzymology |v 426 |w (DE-604)BV000000938 |9 426 | |
| 856 | 4 | 2 | |m Digitalisierung UB Regensburg |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016219272&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-016219272 | ||
Datensatz im Suchindex
| _version_ | 1804137231646982144 |
|---|---|
| adam_text | Contents
Contributors
xiii
Preface
xix
Volumes in Series
xxi
ι.
Quantitative Measurements of Integrin-Mediated
Adhesion to Extracellular Matrix
ι
David Boettiger
ι.
Introduction
2
2.
Spinning Disc Measurements
3
3.
Protocols
4
4.
Data Analysis
12
Acknowledgments
19
References
24
2.
investigating
Integrin
Regulation and Signaling Events in
Three-Dimensional Systems
27
Patricia J. Keely, Matthew W. Conktin, Scott
Gehler,
Suzanne M. Ponik, and Paolo P. Provenzano
1.
Introduction
28
2.
Collagen Matrices of Different Densities
31
3.
Gel Contraction as a Measure to Quantify Cell Contractility
33
4.
Rho Activity Assay from Cells Cultured in
3D
Collagen Gels
33
5.
Co-lmmunoprecipitation of Integrin-Associated Proteins
from Cells Cultured in
3D
Collagen Gels
35
6.
jS^lntegrin Endocytosis Assays
36
7.
Imaging Cell-Matrix Interactions in
3D
Collagen Gels
39
References
44
3.
Integrins
in Cell Migration
47
Keefe T. Chan,
Christa
L
Cortesio, and Anna Huttenlocher
1.
Introduction
47
2.
Methods for Analysis of Integrin-Mediated Cell Migration
49
References
64
vi
Contents
4. Integrin Cytoskeletal
Interactions
69
Yatish
Lad, David
S.
Harburger, and David A. Caiderwood
1.
Introduction
70
2. Models
of
Integrin Cytoplasmic
Tails
72
3. Expression
Constructs for
Integrin
Tail
Model Proteins
74
4.
Purification of
Integrin Cytoplasmic
Tails
74
5.
Preparation of the Affinity Matrix
76
6.
Binding Assays Using Cell Lysates
77
7.
Direct Protein-Protein Binding Assays
78
8.
Advanced Applications
79
9.
Concluding Remarks
81
Acknowledgments
81
References
82
5.
Cell Survival in a Three-Dimensional Matrix
85
Alireza
Alavi
and Dwayne G. Stupack
1.
Introduction
86
2.
Protocols
87
3.
Conclusions and Perspective
100
References
100
6.
Platelet Integrin Adhesive Functions and Signaling
103
Nicolas
Prévost, Hisashi
Kato,
Laurent
Bodin,
and
Sanford J.
Shattil
1.
Introduction
104
2.
Cell Models
104
3.
Microscopy
107
4.
Assaying Integrin Adhesive Responses
108
5.
Biochemical Analysis of Integrin-Based Signaling
111
References i!4
Development of Monoclonal Antibodies to Integrin Receptors
117
E. A. Wayner and
B. G.
Hoffstrom
1.
Introduction
це
2.
Methods
121
3.
Protocols i39
References 151
Contents
vii
8.
Cell Adhesion, Cellular Tension, and Cell Cycle Control
155
Eric A. Klein, Yuval Yung,
Paola Castagnino,
Devashish Kothapalli, and Richard K. Assoian
1.
Introduction
156
2.
Preparative Methods
156
3.
Analytical Methods
165
Acknowledgments
174
References
174
9.
Analysis of
Integrin
Signaling by Fluorescence
Resonance Energy Transfer
177
Yingxiao Wang and Shu
Chien
1.
Introduction
178
2.
Fluorescence Proteins and FRET
179
3.
Integrin
Signaling
180
4.
FRET Analysis of
Integrin
Signaling
182
5.
Future Directions
196
Acknowledgments
197
References
197
10.
Studies on
Integrins
in the Nervous System
203
Sumiko Denda and Louis F. Reichardt
1.
Introduction
204
2. Neuronal
Cell Adhesion and Neurite Outgrowth Assays
208
3. Neuronal
Culture Procedures
210
4.
Biochemical Studies Using Cultured Neurons
214
References
216
11.
Methods for Identifying Novel
Integrin Ligands
223
Denise
К.
Marciano,
Sumiko Denda, and Louis F. Reichardt
1.
Introduction
224
2.
Production of Soluble aS jSi-AP
228
3.
Solid-Phase Binding Assays with Soluble
Integrin Heterodimers
230
4.
Histochemistry with Soluble
Integrin
Heterodimers
231
5.
Far Western Blotting Using
integrin
Heterodimers
231
6.
Ligand Detection Using Intact
Integrin
Receptors
232
7.
аз/?і
Immunolabeling and Purification
233
8.
Receptor-Binding Assays
234
References
234
viii Contents
12.
Analysis of
Integrin
Functions in Peri-Implantation Embryos,
Hematopoietic System, and Skin
239
Eloi Montanez,
Aleksandra Piwko-Czuchra,
Martina Bauer,
Shaohua Li, Peter Yurchenco, and
Reinhard Fässler
1.
Introduction
240
2.
Analysis of
Integrin
Functions during Peri-Implantation Development
241
3.
Analysis of
Integrin
Functions in Blood
249
4.
Analysis of
Integrin
Functions in Skin
■ 264
5.
In Vitro Skin Analysis
277
References
286
13.
Identification and Molecular Characterization of
Multiple Phenotypes in Integrin Knockout Mice
291
Chun Chen and Dean Sheppard
1.
Introduction
292
2.
Strategies for Generating Integrin Knockout Mice
292
3.
Validation of Observed Phenotypes by Transgenic Rescue In Vivo
293
4.
In Vitro Transgenic Rescue
294
5.
Generation of Blocking Mouse Monoclonal Antibodies
that Recognize
Murine
Integrins
294
6.
Selection of Integrin Knockout Lines to Study
295
7.
Selection of Phenotypic Assays
296
8.
Identification of Additional Phenotypes in Integrin
Knockouts that Survive Embryonic Development
297
9.
Identification of Integrin Knockout Phenotypes Based
on Educated Guesses from In Vitro Experiments
299
10.
Use of Expression Microarrays to Suggest
Possible Phenotypic Assays
300
11.
Summary
302
References
302
14.
Purification, Analysis, and Crystal Structure of
Integrins
307
Jian-Ping Xiong, Simon L. Goodman, and M.
Amin
Arnaout
1.
Introduction
308
2.
Purification and Analysis of Integrin Heterodimers
308
3.
Atomic Structure of
Integrins
Using Macromolecular Crystallography
320
4.
Summary
328
Acknowledgments
329
References
329
Contents
¡χ
15. Electron
Microscopy of
Integrins
337
Brian
D. Adair
and Mark Yeager
1.
Introduction
338
2.
Purification of Full-Length
апь^з
from Platelets
339
3.
Preparation of Specimens for Negative-Stain EM
341
4.
Preparation of Specimens for Cryo-EM
346
5.
Low-Dose EM
348
6.
Image Appraisal, Particle Selection, and Preprocessing
350
7.
Initial Particle Analysis and Generation of Reference-Free Aligned
Class Averages
355
8.
Three-Dimensional Structure Refinement
357
9.
Evaluation of the Refinement
362
10.
Pseudoatomic Modeling of EM Density Maps
365
11.
Future Prospects for Cryo-EM
368
Acknowledgments
369
References
369
16.
Intravital
Imaging and Cell Invasion
375
Milan Makale
1.
Introduction
376
2.
Methods
380
Acknowledgments
398
References
398
17.
Using Xenopus Embryos to Investigate
Integrin
Function
403
Douglas W.
DeSimone,
Bette
Dzamba, and Lance A. Davidson
1.
Introduction
404
2.
Visualization of
Integrins,
Extracellular Matrix,
and Cytoskeleton in Embryo
Expiants
405
3.
Live Imaging of Fibronectin Fibrils
410
4.
Conclusions
413
Acknowledgments
413
References
414
18.
Methods to Study Lymphatic Vessel
Integrins
415
Barbara Garmy-Susini, Milan Makale, Mark Fuster, and Judith A. Varner
1.
Introduction
416
2.
Isolation of Human Lymphatic Endothelial Cells
418
3.
Isolation of
Murine
Lymphatic Endothelial Cells
418
Contents
4. Characterization
of
LEC
Integrin Expression 421
5. In Vitro
Cell Adhesion Assays
42α
6.
Migration Assays
423
7. Matrigel
Tube Formation
424
8.
Frozen Section Immunofluorescence Microscopy
425
9.
Paraffin-Embedded Section Immunohistochemistry
427
10.
Growth-Factor-Induced Lymph Node Lymphangiogenesis
428
11.
Murine
Lymphangioma Model
428
12.
Tumor Models
430
13.
Endothelial Cell-Specific Integrin
aą
Deletion Mutant
432
14.
Intravital
Microscopy of Lymph Nodes
433
15.
Abdominal Window Implantation
433
16. Intradermal
and Footpad Injections
436
References
436
19.
Analysis of Integrin Signaling in Genetically Engineered
Mouse Models of Mammary Tumor Progression
439
Yuliya Pylayeva, Wenjun Guo, and
Filippo
G. Giancotti
1.
Introduction
440
2.
Experimental Approach
442
3.
Measurement of Primary Tumor Growth
443
4.
Spontaneous and Allograft Lung Metastasis Assays
444
5.
Analysis of Tumor Sections
448
6.
Detergent Extraction of Tumor Samples
452
7.
£x Vivo Culture of Mammary Tumor Cells
452
8.
Genetic Modification of Primary Mammary Tumor Celts
453
9.
Disruption of Epithelial Adhesion and Growth Control
456
Acknowledgments
459
References
459
20.
Design and Chemical Synthesis of Integrin Ligands
463
Dominik Heckmann
and
Horst Kessler
1.
Introduction
464
2.
Design of Integrin Ligands: Overview
465
3.
Application of Integrin Ligands for Imaging and Surface Coating
480
4.
Experimental Section
488
References
495
Contents xi
21.
Evaluating
Integrin
Function in Models of Angiogenesis and
Vascular Permeability
505
Sara M.
Weis
1.
Introduction
506
2.
Assessing Role of
Integrins
During Angiogenesis
511
3.
Assessing Role of
Integrins
in Vascular Permeability
518
4.
Concluding Remarks
521
References
523
Author Index
529
Subject Index S59
|
| adam_txt |
Contents
Contributors
xiii
Preface
xix
Volumes in Series
xxi
ι.
Quantitative Measurements of Integrin-Mediated
Adhesion to Extracellular Matrix
ι
David Boettiger
ι.
Introduction
2
2.
Spinning Disc Measurements
3
3.
Protocols
4
4.
Data Analysis
12
Acknowledgments
19
References
24
2.
investigating
Integrin
Regulation and Signaling Events in
Three-Dimensional Systems
27
Patricia J. Keely, Matthew W. Conktin, Scott
Gehler,
Suzanne M. Ponik, and Paolo P. Provenzano
1.
Introduction
28
2.
Collagen Matrices of Different Densities
31
3.
Gel Contraction as a Measure to Quantify Cell Contractility
33
4.
Rho Activity Assay from Cells Cultured in
3D
Collagen Gels
33
5.
Co-lmmunoprecipitation of Integrin-Associated Proteins
from Cells Cultured in
3D
Collagen Gels
35
6.
jS^lntegrin Endocytosis Assays
36
7.
Imaging Cell-Matrix Interactions in
3D
Collagen Gels
39
References
44
3.
Integrins
in Cell Migration
47
Keefe T. Chan,
Christa
L
Cortesio, and Anna Huttenlocher
1.
Introduction
47
2.
Methods for Analysis of Integrin-Mediated Cell Migration
49
References
64
vi
Contents
4. Integrin Cytoskeletal
Interactions
69
Yatish
Lad, David
S.
Harburger, and David A. Caiderwood
1.
Introduction
70
2. Models
of
Integrin Cytoplasmic
Tails
72
3. Expression
Constructs for
Integrin
Tail
Model Proteins
74
4.
Purification of
Integrin Cytoplasmic
Tails
74
5.
Preparation of the Affinity Matrix
76
6.
Binding Assays Using Cell Lysates
77
7.
Direct Protein-Protein Binding Assays
78
8.
Advanced Applications
79
9.
Concluding Remarks
81
Acknowledgments
81
References
82
5.
Cell Survival in a Three-Dimensional Matrix
85
Alireza
Alavi
and Dwayne G. Stupack
1.
Introduction
86
2.
Protocols
87
3.
Conclusions and Perspective
100
References
100
6.
Platelet Integrin Adhesive Functions and Signaling
103
Nicolas
Prévost, Hisashi
Kato,
Laurent
Bodin,
and
Sanford J.
Shattil
1.
Introduction
104
2.
Cell Models
104
3.
Microscopy
107
4.
Assaying Integrin Adhesive Responses
108
5.
Biochemical Analysis of Integrin-Based Signaling
111
References i!4
Development of Monoclonal Antibodies to Integrin Receptors
117
E. A. Wayner and
B. G.
Hoffstrom
1.
Introduction
це
2.
Methods
121
3.
Protocols i39
References 151
Contents
vii
8.
Cell Adhesion, Cellular Tension, and Cell Cycle Control
155
Eric A. Klein, Yuval Yung,
Paola Castagnino,
Devashish Kothapalli, and Richard K. Assoian
1.
Introduction
156
2.
Preparative Methods
156
3.
Analytical Methods
165
Acknowledgments
174
References
174
9.
Analysis of
Integrin
Signaling by Fluorescence
Resonance Energy Transfer
177
Yingxiao Wang and Shu
Chien
1.
Introduction
178
2.
Fluorescence Proteins and FRET
179
3.
Integrin
Signaling
180
4.
FRET Analysis of
Integrin
Signaling
182
5.
Future Directions
196
Acknowledgments
197
References
197
10.
Studies on
Integrins
in the Nervous System
203
Sumiko Denda and Louis F. Reichardt
1.
Introduction
204
2. Neuronal
Cell Adhesion and Neurite Outgrowth Assays
208
3. Neuronal
Culture Procedures
210
4.
Biochemical Studies Using Cultured Neurons
214
References
216
11.
Methods for Identifying Novel
Integrin Ligands
223
Denise
К.
Marciano,
Sumiko Denda, and Louis F. Reichardt
1.
Introduction
224
2.
Production of Soluble aS'jSi-AP
228
3.
Solid-Phase Binding Assays with Soluble
Integrin Heterodimers
230
4.
Histochemistry with Soluble
Integrin
Heterodimers
231
5.
Far Western Blotting Using
integrin
Heterodimers
231
6.
Ligand Detection Using Intact
Integrin
Receptors
232
7.
аз/?і
Immunolabeling and Purification
233
8.
Receptor-Binding Assays
234
References
234
viii Contents
12.
Analysis of
Integrin
Functions in Peri-Implantation Embryos,
Hematopoietic System, and Skin
239
Eloi Montanez,
Aleksandra Piwko-Czuchra,
Martina Bauer,
Shaohua Li, Peter Yurchenco, and
Reinhard Fässler
1.
Introduction
240
2.
Analysis of
Integrin
Functions during Peri-Implantation Development
241
3.
Analysis of
Integrin
Functions in Blood
249
4.
Analysis of
Integrin
Functions in Skin
■ 264
5.
In Vitro Skin Analysis
277
References
286
13.
Identification and Molecular Characterization of
Multiple Phenotypes in Integrin Knockout Mice
291
Chun Chen and Dean Sheppard
1.
Introduction
292
2.
Strategies for Generating Integrin Knockout Mice
292
3.
Validation of Observed Phenotypes by Transgenic Rescue In Vivo
293
4.
In Vitro Transgenic Rescue
294
5.
Generation of Blocking Mouse Monoclonal Antibodies
that Recognize
Murine
Integrins
294
6.
Selection of Integrin Knockout Lines to Study
295
7.
Selection of Phenotypic Assays
296
8.
Identification of Additional Phenotypes in Integrin
Knockouts that Survive Embryonic Development
297
9.
Identification of Integrin Knockout Phenotypes Based
on Educated Guesses from In Vitro Experiments
299
10.
Use of Expression Microarrays to Suggest
Possible Phenotypic Assays
300
11.
Summary
302
References
302
14.
Purification, Analysis, and Crystal Structure of
Integrins
307
Jian-Ping Xiong, Simon L. Goodman, and M.
Amin
Arnaout
1.
Introduction
308
2.
Purification and Analysis of Integrin Heterodimers
308
3.
Atomic Structure of
Integrins
Using Macromolecular Crystallography
320
4.
Summary
328
Acknowledgments
329
References
329
Contents
¡χ
15. Electron
Microscopy of
Integrins
337
Brian
D. Adair
and Mark Yeager
1.
Introduction
338
2.
Purification of Full-Length
апь^з
from Platelets
339
3.
Preparation of Specimens for Negative-Stain EM
341
4.
Preparation of Specimens for Cryo-EM
346
5.
Low-Dose EM
348
6.
Image Appraisal, Particle Selection, and Preprocessing
350
7.
Initial Particle Analysis and Generation of Reference-Free Aligned
Class Averages
355
8.
Three-Dimensional Structure Refinement
357
9.
Evaluation of the Refinement
362
10.
Pseudoatomic Modeling of EM Density Maps
365
11.
Future Prospects for Cryo-EM
368
Acknowledgments
369
References
369
16.
Intravital
Imaging and Cell Invasion
375
Milan Makale
1.
Introduction
376
2.
Methods
380
Acknowledgments
398
References
398
17.
Using Xenopus Embryos to Investigate
Integrin
Function
403
Douglas W.
DeSimone,
Bette
Dzamba, and Lance A. Davidson
1.
Introduction
404
2.
Visualization of
Integrins,
Extracellular Matrix,
and Cytoskeleton in Embryo
Expiants
405
3.
Live Imaging of Fibronectin Fibrils
410
4.
Conclusions
413
Acknowledgments
413
References
414
18.
Methods to Study Lymphatic Vessel
Integrins
415
Barbara Garmy-Susini, Milan Makale, Mark Fuster, and Judith A. Varner
1.
Introduction
416
2.
Isolation of Human Lymphatic Endothelial Cells
418
3.
Isolation of
Murine
Lymphatic Endothelial Cells
418
Contents
4. Characterization
of
LEC
Integrin Expression 421
5. In Vitro
Cell Adhesion Assays
42α
6.
Migration Assays
423
7. Matrigel
Tube Formation
424
8.
Frozen Section Immunofluorescence Microscopy
425
9.
Paraffin-Embedded Section Immunohistochemistry
427
10.
Growth-Factor-Induced Lymph Node Lymphangiogenesis
428
11.
Murine
Lymphangioma Model
428
12.
Tumor Models
430
13.
Endothelial Cell-Specific Integrin
aą
Deletion Mutant
432
14.
Intravital
Microscopy of Lymph Nodes
433
15.
Abdominal Window Implantation
433
16. Intradermal
and Footpad Injections
436
References
436
19.
Analysis of Integrin Signaling in Genetically Engineered
Mouse Models of Mammary Tumor Progression
439
Yuliya Pylayeva, Wenjun Guo, and
Filippo
G. Giancotti
1.
Introduction
440
2.
Experimental Approach
442
3.
Measurement of Primary Tumor Growth
443
4.
Spontaneous and Allograft Lung Metastasis Assays
444
5.
Analysis of Tumor Sections
448
6.
Detergent Extraction of Tumor Samples
452
7.
£x Vivo Culture of Mammary Tumor Cells
452
8.
Genetic Modification of Primary Mammary Tumor Celts
453
9.
Disruption of Epithelial Adhesion and Growth Control
456
Acknowledgments
459
References
459
20.
Design and Chemical Synthesis of Integrin Ligands
463
Dominik Heckmann
and
Horst Kessler
1.
Introduction
464
2.
Design of Integrin Ligands: Overview
465
3.
Application of Integrin Ligands for Imaging and Surface Coating
480
4.
Experimental Section
488
References
495
Contents xi
21.
Evaluating
Integrin
Function in Models of Angiogenesis and
Vascular Permeability
505
Sara M.
Weis
1.
Introduction
506
2.
Assessing Role of
Integrins
During Angiogenesis
511
3.
Assessing Role of
Integrins
in Vascular Permeability
518
4.
Concluding Remarks
521
References
523
Author Index
529
Subject Index S59 |
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| any_adam_object_boolean | 1 |
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| bvnumber | BV023015105 |
| callnumber-first | Q - Science |
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| discipline_str_mv | Biologie Chemie Medizin |
| format | Book |
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| genre | (DE-588)4143413-4 Aufsatzsammlung gnd-content |
| genre_facet | Aufsatzsammlung |
| id | DE-604.BV023015105 |
| illustrated | Illustrated |
| index_date | 2024-07-02T19:10:57Z |
| indexdate | 2024-07-09T21:09:01Z |
| institution | BVB |
| isbn | 9780123739247 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-016219272 |
| oclc_num | 173498265 |
| open_access_boolean | |
| owner | DE-12 DE-355 DE-BY-UBR DE-91G DE-BY-TUM DE-19 DE-BY-UBM DE-20 |
| owner_facet | DE-12 DE-355 DE-BY-UBR DE-91G DE-BY-TUM DE-19 DE-BY-UBM DE-20 |
| physical | XLV, 567, [12] Bl. Ill., graph. Darst. |
| publishDate | 2007 |
| publishDateSearch | 2007 |
| publishDateSort | 2007 |
| publisher | Elsevier, Acad. Press |
| record_format | marc |
| series | Methods in enzymology |
| series2 | Methods in enzymology |
| spelling | Integrins ed. by David A. Cheresh Amsterdam [u.a.] Elsevier, Acad. Press 2007 XLV, 567, [12] Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in enzymology 426 Integrins Integrine (DE-588)4278201-6 gnd rswk-swf Labortechnik (DE-588)4123602-6 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Integrine (DE-588)4278201-6 s Labortechnik (DE-588)4123602-6 s DE-604 Cheresh, David A. Sonstige oth Methods in enzymology 426 (DE-604)BV000000938 426 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016219272&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Integrins Methods in enzymology Integrins Integrine (DE-588)4278201-6 gnd Labortechnik (DE-588)4123602-6 gnd |
| subject_GND | (DE-588)4278201-6 (DE-588)4123602-6 (DE-588)4143413-4 |
| title | Integrins |
| title_auth | Integrins |
| title_exact_search | Integrins |
| title_exact_search_txtP | Integrins |
| title_full | Integrins ed. by David A. Cheresh |
| title_fullStr | Integrins ed. by David A. Cheresh |
| title_full_unstemmed | Integrins ed. by David A. Cheresh |
| title_short | Integrins |
| title_sort | integrins |
| topic | Integrins Integrine (DE-588)4278201-6 gnd Labortechnik (DE-588)4123602-6 gnd |
| topic_facet | Integrins Integrine Labortechnik Aufsatzsammlung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016219272&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV000000938 |
| work_keys_str_mv | AT chereshdavida integrins |