Lipidomics and bioactive lipids: Mass-spectrometry-based lipid analysis:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Amsterdam [u.a.]
Elsevier, Acad. Press
2007
|
| Schriftenreihe: | Methods in enzymology
432 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XLIV, 387 S., [19] Bl. Ill., graph. Darst. |
| ISBN: | 9780123738950 0123738954 |
Internformat
MARC
| LEADER | 00000nam a2200000 cb4500 | ||
|---|---|---|---|
| 001 | BV022964631 | ||
| 003 | DE-604 | ||
| 005 | 20080228 | ||
| 007 | t | ||
| 008 | 071114s2007 ad|| |||| 00||| eng d | ||
| 020 | |a 9780123738950 |9 978-0-12-373895-0 | ||
| 020 | |a 0123738954 |9 0-12-373895-4 | ||
| 035 | |a (OCoLC)222132522 | ||
| 035 | |a (DE-599)BVBBV022964631 | ||
| 040 | |a DE-604 |b ger |e rakwb | ||
| 041 | 0 | |a eng | |
| 049 | |a DE-355 |a DE-19 |a DE-20 |a DE-91G |a DE-12 |a DE-29T | ||
| 082 | 0 | |a 547.77046 |2 22 | |
| 084 | |a WC 4355 |0 (DE-625)148096: |2 rvk | ||
| 084 | |a CHE 840f |2 stub | ||
| 245 | 1 | 0 | |a Lipidomics and bioactive lipids: Mass-spectrometry-based lipid analysis |c ed. by H. Alex Brown |
| 264 | 1 | |a Amsterdam [u.a.] |b Elsevier, Acad. Press |c 2007 | |
| 300 | |a XLIV, 387 S., [19] Bl. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in enzymology |v 432 | |
| 650 | 4 | |a Lipids |x analysis | |
| 650 | 4 | |a Mass Spectrometry |x methods | |
| 650 | 0 | 7 | |a Labortechnik |0 (DE-588)4123602-6 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Lipidstoffwechsel |0 (DE-588)4035875-6 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Massenspektrometrie |0 (DE-588)4037882-2 |2 gnd |9 rswk-swf |
| 689 | 0 | 0 | |a Lipidstoffwechsel |0 (DE-588)4035875-6 |D s |
| 689 | 0 | 1 | |a Massenspektrometrie |0 (DE-588)4037882-2 |D s |
| 689 | 0 | 2 | |a Labortechnik |0 (DE-588)4123602-6 |D s |
| 689 | 0 | |C b |5 DE-604 | |
| 700 | 1 | |a Brown, H. Alex |e Sonstige |4 oth | |
| 830 | 0 | |a Methods in enzymology |v 432 |w (DE-604)BV000000938 |9 432 | |
| 856 | 4 | 2 | |m Digitalisierung UB Regensburg |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016168960&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-016168960 | ||
Datensatz im Suchindex
| _version_ | 1804137213332553728 |
|---|---|
| adam_text | Contents
Contributors
xi
Preface
xvii
Volumes in Series
xix
ι.
Qualitative Analysis and Quantitative Assessment of Changes in
Neutral Glycerol
Lipid
Molecular Species Within Cells
ι
Jessica Krank,
Robert
С
Murphy, Robert M. Barkley, Eva Duchoslav, and
Andrew McAnoy
ι.
Introduction
2
2.
Reagents
3
3.
Methods
4
4.
Results
7
5.
Conclusions
19
Acknowledgments
19
References
19
2.
Glycerophospholipid Identification and Quantitation by Electrospray
lonization Mass Spectrometry
21
Pavlina
T.
Ivanova,
Stephen
В.
Milne, Mark
О.
Byrne, Yun Xiang, and
H. Alex Brown
1.
Introduction
22
2.
Nomenclature
25
3.
Mass Spectrometry
26
4.
General Strategy for Phospholipid Isolation and Mass Spectral Analysis
27
5.
Extraction and Mass Spectral Analysis of Global Glycerophospholipids
28
6.
Polyphosphoinositide Extraction and Mass Spectral Analysis
35
7.
Computational Analysis of Mass Spectral Data
41
Acknowledgments
54
References
54
VI
Contents
3.
Detection and Quantitation of Eicosanoids via High Performance
Liquid Chromatography-Electrospray lonization-Mass Spectrometry
59
Raymond Deems, Matthew W. Buczynski, Rebecca Bowers-Gentry,
Richard Harkewicz, and Edward A. Dennis
1.
Introduction
60
2.
Methods
62
3.
Results and Discussion
74
Acknowledgments
80
References
81
4.
Structure-Specific, Quantitative Methods for Analysis of Sphingolipids
by Liquid Chromatography-Tandem Mass Spectrometry:
Inside-Out Sphingolipidomics
83
M. Cameron Sullards, Jeremy
С
Allegood, Samuel Kelly, Elaine Wang,
Christopher A. Haynes, Hyejung Park, Yanfeng Chen, and Alfred H. Merrill, Jr.
1.
Introduction: An Overview of Sphingolipid Structures and Nomenclature
86
2.
Analysis of Sphingolipids by Mass Spectrometry
89
3.
Analysis of Sphingolipids by OMIC Approaches
92
4.
Materials and Methods
95
5.
Materials
98
6.
Extraction
98
7.
Identification of the Molecular Species by Tandem Mass Spectrometry
100
8.
Quantitation by LC-ESI-MS/MS Using Multireaction Monitoring
101
9.
Analysis of (Dihydro)Ceramides, (Dihydro)Sphingomyelins, and
(DihydrojMonohexosyl-Ceramides in Positive Ion Mode
104
10.
Other Methods
109
Acknowledgments
111
References
112
5.
Analysis of Ubiquinones, DoUchols, and Dolichol Diphosphate-
Oligosaccharides by Liquid Chromatography-Electrospray
lonization-Mass Spectrometry
117
Teresa A. Garrett, Ziqiang Guan, and Christian R. H. Raetz
1.
Introduction
118
2.
Materials
121
3.
Liquid Chromatography-Mass Spectrometry
122
4.
Preparation of
Lipid
Extracts
122
5.
LC-MS Detection and Quantification of Coenzyme
Q
123
6.
LC-MS Detection and Quantification of Dolichol
130
Contents
vii
7. LC-MS
and LC-MS/MS Characterization of Dolichol
Diphosphate-Linked Oligosaccharides 13c
Acknowledgment
140
References
140
6.
Extraction and Analysis of Sterols in Biological Matrices by High
Performance Liquid Chromatography Electrospray lonization
Mass Spectrometry
145
Jeffrey G. McDonald, Bonne M. Thompson, Erin
С
McCrum, and
David W. Russell
1.
Introduction
146
2.
Supplies and Reagents
148
3.
Extraction of Lipids from Cultured Cells and Tissues
149
4.
Saponification
of
Lipid
Extracts
151
5.
Solid-Phase Extraction
152
6.
Analysis by HPLC-ESI-MS
153
7.
Quantitation
156
8.
Data
157
9.
Discussion, Nuances, Caveats, and Pitfalls
162
Acknowledgments
168
References
169
7.
The
Lipid
Maps Initiative in Lipidomics
171
Kara
Schmelzer,
Eoin Fahy, Shankar Subramaniam, and Edward A. Dennis
1.
Introduction
172
2.
Building Infrastructure in Lipidomics
173
3.
Classification, Nomenclature, and Structural Representation of Lipids
174
4.
Mass Spectrometry as a Platform for
Lipid
Molecular Species
177
5.
Future Plans
180
Acknowledgments
182
References
182
8.
Basic Analytical Systems for Lipidomics by Mass Spectrometry
in Japan
185
Ryo Taguchi, Mashahiro Nishijima, and Takao Shimizu
1.
Introduction
186
2.
Lipid
Bank and Related Databases
187
3.
Strategies for
Lipid
Identification and Quantitative Analysis by
Mass Spectrometry
188
viii Contents
4.
Several Practical Lipidomics Methods by Mass Spectrometry
190
5.
Strategies for Identification of Individual Molecular Species in
Glycerolipids and Glycerophospholipids by
Lipid
Search
192
6.
Quantitative Analysis or Profiling of
Lipid
Molecular Species
197
7.
Application of Several Different Methods in Lipidomics
199
8.
Future Program for Lipidomics
208
Acknowledgments
209
References
209
9.
The European Lipidomics Initiative: Enabling Technologies
213
Gerrit
van
Meer,
Bas R. Leeflang,
Gerhard
Liebisch, Gerd Schmitz,
and
Felix
M.
Goni
1.
Introduction
214
2.
Methods of Lipidomics: Quantitative Analysis in Time
215
3.
Imaging Lipids
218
4.
Methods to Study the Physical Properties of Lipids
219
5.
Data Handling and Standardization
224
6.
Perspectives
226
Acknowledgments
227
References
227
10.
Lipidomic Analysis of Signaling Pathways
233
Michael J.
0.
Wakelam, Trevor R. Pettitt, and Anthony D. Postle
1.
General Considerations
235
2.
Lipid
Extraction
235
3.
H PLC-MS
Analysis
237
4.
General Phospholipid HPLC
237
5.
Ceramide, Diradylglycerol, and Monoradylglycerol Separation
239
6.
Phosphoinositide Separation
240
7.
Analysis of Phospholipid Synthesis by ESI-MS/MS Using
Stable Isotopes
241
8.
Labeling Protocols
242
9.
Lipid
Extraction for ESI-MS/MS
243
10.
LC-MS Analysis (methyl-dgJ-Choline
243
11.
MRM Analysis of (/nerftyW^-Choline Enrichment in Cell and
Tissue Phosphorylcholine
244
12.
ESI-MS/MS Analysis of Native and Newly Synthesized Phospholipids
244
13.
Data Analysis
245
References
246
Contents ix
11. Bioinformatics
for Lipidomics
247
Eoin
Fahy, Dawn Cotter,
Robert Byrnes, Manish Sud, Andrea Maer, Joshua
Li,
David Nadeau, Yihua Zhau, and Shankar Subramaniam
1.
Introduction
248
2.
Lipid
Structure Databases
249
3.
Lipid-Associated Protein/Gene Databases
254
4.
Tools for Lipidomics
256
5.
Lipid
Pathways
268
6.
Challenges for Future
Lipid
Informatics
270
Acknowledgments
272
References
272
12.
Mediator Lipidomics: Search Algorithms for Eicosanoids, Resolvins,
and Protectins
275
Charles
N.
Serhan, Yan
Lu, Song
Hong, and RongYang
1.
Introduction: Metabolomics and Mediator Lipidomics-lnformatics
276
2.
Engineered Animals and Human Tissues
278
3.
New Chemical Mediator Pathways in Resolution of Inflammation
279
4.
Logic Diagram to Identify PUFA-Derived
Lipid
Mediators: Eicosanoids,
Resolvins, and Protectins
284
5.
Cognoscitive-Contrast-Angle Algorithm
286
6.
Theoretical Database for Novel LM and Search Algorithm
292
7.
Discussion
308
8.
Conclusions and Next Steps
311
Acknowledgments
313
References
313
13.
A Guide to Biochemical Systems Modeling of Sphingolipids for
the Biochemist
319
Kellie
J.
Sims, Fernando Alvarez-Vasquez,
Eberhard O.
Voit,
and
Yusuf A. Hannun
1.
Introduction
320
2.
System Map: Specifying the Model
322
3.
Symbolic Equations: From Words and Pictures to Equations
327
4.
Numerical Equations: From the Symbolic to a Computational Model
331
5.
Model Analysis: Steady State, Stability, and Sensitivity
341
6.
Simulation: What Happens If.
..? 345
7.
Conclusion
346
χ
Contents
8.
Epilogue
346
Acknowledgments
348
References
348
14.
Quantitation and Standardization of Lipid Internal Standards for
Mass Spectroscopy
351
Jeff D. Moore, William V. Caufield, and Walter A. Shaw
1.
Introduction
352
2.
Lipid Handling Guidelines
352
3.
Chemical Characterization of Lipid Stocks
353
4.
Preparation of Working Lipid Standards
355
5.
Packaging of Lipid Standards
359
6.
Quality Control and Stability Testing
361
7.
Discussion
364
References
366
Author Index
36g
Subject Index
381
|
| adam_txt |
Contents
Contributors
xi
Preface
xvii
Volumes in Series
xix
ι.
Qualitative Analysis and Quantitative Assessment of Changes in
Neutral Glycerol
Lipid
Molecular Species Within Cells
ι
Jessica Krank,
Robert
С
Murphy, Robert M. Barkley, Eva Duchoslav, and
Andrew McAnoy
ι.
Introduction
2
2.
Reagents
3
3.
Methods
4
4.
Results
7
5.
Conclusions
19
Acknowledgments
19
References
19
2.
Glycerophospholipid Identification and Quantitation by Electrospray
lonization Mass Spectrometry
21
Pavlina
T.
Ivanova,
Stephen
В.
Milne, Mark
О.
Byrne, Yun Xiang, and
H. Alex Brown
1.
Introduction
22
2.
Nomenclature
25
3.
Mass Spectrometry
26
4.
General Strategy for Phospholipid Isolation and Mass Spectral Analysis
27
5.
Extraction and Mass Spectral Analysis of Global Glycerophospholipids
28
6.
Polyphosphoinositide Extraction and Mass Spectral Analysis
35
7.
Computational Analysis of Mass Spectral Data
41
Acknowledgments
54
References
54
VI
Contents
3.
Detection and Quantitation of Eicosanoids via High Performance
Liquid Chromatography-Electrospray lonization-Mass Spectrometry
59
Raymond Deems, Matthew W. Buczynski, Rebecca Bowers-Gentry,
Richard Harkewicz, and Edward A. Dennis
1.
Introduction
60
2.
Methods
62
3.
Results and Discussion
74
Acknowledgments
80
References
81
4.
Structure-Specific, Quantitative Methods for Analysis of Sphingolipids
by Liquid Chromatography-Tandem Mass Spectrometry:
"Inside-Out" Sphingolipidomics
83
M. Cameron Sullards, Jeremy
С
Allegood, Samuel Kelly, Elaine Wang,
Christopher A. Haynes, Hyejung Park, Yanfeng Chen, and Alfred H. Merrill, Jr.
1.
Introduction: An Overview of Sphingolipid Structures and Nomenclature
86
2.
Analysis of Sphingolipids by Mass Spectrometry
89
3.
Analysis of Sphingolipids by "OMIC" Approaches
92
4.
Materials and Methods
95
5.
Materials
98
6.
Extraction
98
7.
Identification of the Molecular Species by Tandem Mass Spectrometry
100
8.
Quantitation by LC-ESI-MS/MS Using Multireaction Monitoring
101
9.
Analysis of (Dihydro)Ceramides, (Dihydro)Sphingomyelins, and
(DihydrojMonohexosyl-Ceramides in Positive Ion Mode
104
10.
Other Methods
109
Acknowledgments
111
References
112
5.
Analysis of Ubiquinones, DoUchols, and Dolichol Diphosphate-
Oligosaccharides by Liquid Chromatography-Electrospray
lonization-Mass Spectrometry
117
Teresa A. Garrett, Ziqiang Guan, and Christian R. H. Raetz
1.
Introduction
118
2.
Materials
121
3.
Liquid Chromatography-Mass Spectrometry
122
4.
Preparation of
Lipid
Extracts
122
5.
LC-MS Detection and Quantification of Coenzyme
Q
123
6.
LC-MS Detection and Quantification of Dolichol
130
Contents
vii
7. LC-MS
and LC-MS/MS Characterization of Dolichol
Diphosphate-Linked Oligosaccharides 13c
Acknowledgment
140
References
140
6.
Extraction and Analysis of Sterols in Biological Matrices by High
Performance Liquid Chromatography Electrospray lonization
Mass Spectrometry
145
Jeffrey G. McDonald, Bonne M. Thompson, Erin
С
McCrum, and
David W. Russell
1.
Introduction
146
2.
Supplies and Reagents
148
3.
Extraction of Lipids from Cultured Cells and Tissues
149
4.
Saponification
of
Lipid
Extracts
151
5.
Solid-Phase Extraction
152
6.
Analysis by HPLC-ESI-MS
153
7.
Quantitation
156
8.
Data
157
9.
Discussion, Nuances, Caveats, and Pitfalls
162
Acknowledgments
168
References
169
7.
The
Lipid
Maps Initiative in Lipidomics
171
Kara
Schmelzer,
Eoin Fahy, Shankar Subramaniam, and Edward A. Dennis
1.
Introduction
172
2.
Building Infrastructure in Lipidomics
173
3.
Classification, Nomenclature, and Structural Representation of Lipids
174
4.
Mass Spectrometry as a Platform for
Lipid
Molecular Species
177
5.
Future Plans
180
Acknowledgments
182
References
182
8.
Basic Analytical Systems for Lipidomics by Mass Spectrometry
in Japan
185
Ryo Taguchi, Mashahiro Nishijima, and Takao Shimizu
1.
Introduction
186
2.
Lipid
Bank and Related Databases
187
3.
Strategies for
Lipid
Identification and Quantitative Analysis by
Mass Spectrometry
188
viii Contents
4.
Several Practical Lipidomics Methods by Mass Spectrometry
190
5.
Strategies for Identification of Individual Molecular Species in
Glycerolipids and Glycerophospholipids by
Lipid
Search
192
6.
Quantitative Analysis or Profiling of
Lipid
Molecular Species
197
7.
Application of Several Different Methods in Lipidomics
199
8.
Future Program for Lipidomics
208
Acknowledgments
209
References
209
9.
The European Lipidomics Initiative: Enabling Technologies
213
Gerrit
van
Meer,
Bas R. Leeflang,
Gerhard
Liebisch, Gerd Schmitz,
and
Felix
M.
Goni
1.
Introduction
214
2.
Methods of Lipidomics: Quantitative Analysis in Time
215
3.
Imaging Lipids
218
4.
Methods to Study the Physical Properties of Lipids
219
5.
Data Handling and Standardization
224
6.
Perspectives
226
Acknowledgments
227
References
227
10.
Lipidomic Analysis of Signaling Pathways
233
Michael J.
0.
Wakelam, Trevor R. Pettitt, and Anthony D. Postle
1.
General Considerations
235
2.
Lipid
Extraction
235
3.
H PLC-MS
Analysis
237
4.
General Phospholipid HPLC
237
5.
Ceramide, Diradylglycerol, and Monoradylglycerol Separation
239
6.
Phosphoinositide Separation
240
7.
Analysis of Phospholipid Synthesis by ESI-MS/MS Using
Stable Isotopes
241
8.
Labeling Protocols
242
9.
Lipid
Extraction for ESI-MS/MS
243
10.
LC-MS Analysis (methyl-dgJ-Choline
243
11.
MRM Analysis of (/nerftyW^-Choline Enrichment in Cell and
Tissue Phosphorylcholine
244
12.
ESI-MS/MS Analysis of Native and Newly Synthesized Phospholipids
244
13.
Data Analysis
245
References
246
Contents ix
11. Bioinformatics
for Lipidomics
247
Eoin
Fahy, Dawn Cotter,
Robert Byrnes, Manish Sud, Andrea Maer, Joshua
Li,
David Nadeau, Yihua Zhau, and Shankar Subramaniam
1.
Introduction
248
2.
Lipid
Structure Databases
249
3.
Lipid-Associated Protein/Gene Databases
254
4.
Tools for Lipidomics
256
5.
Lipid
Pathways
268
6.
Challenges for Future
Lipid
Informatics
270
Acknowledgments
272
References
272
12.
Mediator Lipidomics: Search Algorithms for Eicosanoids, Resolvins,
and Protectins
275
Charles
N.
Serhan, Yan
Lu, Song
Hong, and RongYang
1.
Introduction: Metabolomics and Mediator Lipidomics-lnformatics
276
2.
Engineered Animals and Human Tissues
278
3.
New Chemical Mediator Pathways in Resolution of Inflammation
279
4.
Logic Diagram to Identify PUFA-Derived
Lipid
Mediators: Eicosanoids,
Resolvins, and Protectins
284
5.
Cognoscitive-Contrast-Angle Algorithm
286
6.
Theoretical Database for Novel LM and Search Algorithm
292
7.
Discussion
308
8.
Conclusions and Next Steps
311
Acknowledgments
313
References
313
13.
A Guide to Biochemical Systems Modeling of Sphingolipids for
the Biochemist
319
Kellie
J.
Sims, Fernando Alvarez-Vasquez,
Eberhard O.
Voit,
and
Yusuf A. Hannun
1.
Introduction
320
2.
System Map: Specifying the Model
322
3.
Symbolic Equations: From Words and Pictures to Equations
327
4.
Numerical Equations: From the Symbolic to a Computational Model
331
5.
Model Analysis: Steady State, Stability, and Sensitivity
341
6.
Simulation: What Happens If.
.? 345
7.
Conclusion
346
χ
Contents
8.
Epilogue
346
Acknowledgments
348
References
348
14.
Quantitation and Standardization of Lipid Internal Standards for
Mass Spectroscopy
351
Jeff D. Moore, William V. Caufield, and Walter A. Shaw
1.
Introduction
352
2.
Lipid Handling Guidelines
352
3.
Chemical Characterization of Lipid Stocks
353
4.
Preparation of Working Lipid Standards
355
5.
Packaging of Lipid Standards
359
6.
Quality Control and Stability Testing
361
7.
Discussion
364
References
366
Author Index
36g
Subject Index
381 |
| any_adam_object | 1 |
| any_adam_object_boolean | 1 |
| building | Verbundindex |
| bvnumber | BV022964631 |
| classification_rvk | WC 4355 |
| classification_tum | CHE 840f |
| ctrlnum | (OCoLC)222132522 (DE-599)BVBBV022964631 |
| dewey-full | 547.77046 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 547 - Organic chemistry |
| dewey-raw | 547.77046 |
| dewey-search | 547.77046 |
| dewey-sort | 3547.77046 |
| dewey-tens | 540 - Chemistry and allied sciences |
| discipline | Chemie / Pharmazie Biologie Chemie |
| discipline_str_mv | Chemie / Pharmazie Biologie Chemie |
| format | Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01808nam a2200445 cb4500</leader><controlfield tag="001">BV022964631</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20080228 </controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">071114s2007 ad|| |||| 00||| eng d</controlfield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9780123738950</subfield><subfield code="9">978-0-12-373895-0</subfield></datafield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">0123738954</subfield><subfield code="9">0-12-373895-4</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)222132522</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)BVBBV022964631</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-355</subfield><subfield code="a">DE-19</subfield><subfield code="a">DE-20</subfield><subfield code="a">DE-91G</subfield><subfield code="a">DE-12</subfield><subfield code="a">DE-29T</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">547.77046</subfield><subfield code="2">22</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WC 4355</subfield><subfield code="0">(DE-625)148096:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">CHE 840f</subfield><subfield code="2">stub</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Lipidomics and bioactive lipids: Mass-spectrometry-based lipid analysis</subfield><subfield code="c">ed. by H. Alex Brown</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Amsterdam [u.a.]</subfield><subfield code="b">Elsevier, Acad. Press</subfield><subfield code="c">2007</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">XLIV, 387 S., [19] Bl.</subfield><subfield code="b">Ill., graph. Darst.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="1" ind2=" "><subfield code="a">Methods in enzymology</subfield><subfield code="v">432</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Lipids</subfield><subfield code="x">analysis</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Mass Spectrometry</subfield><subfield code="x">methods</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Labortechnik</subfield><subfield code="0">(DE-588)4123602-6</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Lipidstoffwechsel</subfield><subfield code="0">(DE-588)4035875-6</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Massenspektrometrie</subfield><subfield code="0">(DE-588)4037882-2</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Lipidstoffwechsel</subfield><subfield code="0">(DE-588)4035875-6</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Massenspektrometrie</subfield><subfield code="0">(DE-588)4037882-2</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="2"><subfield code="a">Labortechnik</subfield><subfield code="0">(DE-588)4123602-6</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="C">b</subfield><subfield code="5">DE-604</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Brown, H. Alex</subfield><subfield code="e">Sonstige</subfield><subfield code="4">oth</subfield></datafield><datafield tag="830" ind1=" " ind2="0"><subfield code="a">Methods in enzymology</subfield><subfield code="v">432</subfield><subfield code="w">(DE-604)BV000000938</subfield><subfield code="9">432</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">Digitalisierung UB Regensburg</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016168960&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-016168960</subfield></datafield></record></collection> |
| id | DE-604.BV022964631 |
| illustrated | Illustrated |
| index_date | 2024-07-02T19:06:06Z |
| indexdate | 2024-07-09T21:08:43Z |
| institution | BVB |
| isbn | 9780123738950 0123738954 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-016168960 |
| oclc_num | 222132522 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-19 DE-BY-UBM DE-20 DE-91G DE-BY-TUM DE-12 DE-29T |
| owner_facet | DE-355 DE-BY-UBR DE-19 DE-BY-UBM DE-20 DE-91G DE-BY-TUM DE-12 DE-29T |
| physical | XLIV, 387 S., [19] Bl. Ill., graph. Darst. |
| publishDate | 2007 |
| publishDateSearch | 2007 |
| publishDateSort | 2007 |
| publisher | Elsevier, Acad. Press |
| record_format | marc |
| series | Methods in enzymology |
| series2 | Methods in enzymology |
| spelling | Lipidomics and bioactive lipids: Mass-spectrometry-based lipid analysis ed. by H. Alex Brown Amsterdam [u.a.] Elsevier, Acad. Press 2007 XLIV, 387 S., [19] Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in enzymology 432 Lipids analysis Mass Spectrometry methods Labortechnik (DE-588)4123602-6 gnd rswk-swf Lipidstoffwechsel (DE-588)4035875-6 gnd rswk-swf Massenspektrometrie (DE-588)4037882-2 gnd rswk-swf Lipidstoffwechsel (DE-588)4035875-6 s Massenspektrometrie (DE-588)4037882-2 s Labortechnik (DE-588)4123602-6 s b DE-604 Brown, H. Alex Sonstige oth Methods in enzymology 432 (DE-604)BV000000938 432 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016168960&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Lipidomics and bioactive lipids: Mass-spectrometry-based lipid analysis Methods in enzymology Lipids analysis Mass Spectrometry methods Labortechnik (DE-588)4123602-6 gnd Lipidstoffwechsel (DE-588)4035875-6 gnd Massenspektrometrie (DE-588)4037882-2 gnd |
| subject_GND | (DE-588)4123602-6 (DE-588)4035875-6 (DE-588)4037882-2 |
| title | Lipidomics and bioactive lipids: Mass-spectrometry-based lipid analysis |
| title_auth | Lipidomics and bioactive lipids: Mass-spectrometry-based lipid analysis |
| title_exact_search | Lipidomics and bioactive lipids: Mass-spectrometry-based lipid analysis |
| title_exact_search_txtP | Lipidomics and bioactive lipids: Mass-spectrometry-based lipid analysis |
| title_full | Lipidomics and bioactive lipids: Mass-spectrometry-based lipid analysis ed. by H. Alex Brown |
| title_fullStr | Lipidomics and bioactive lipids: Mass-spectrometry-based lipid analysis ed. by H. Alex Brown |
| title_full_unstemmed | Lipidomics and bioactive lipids: Mass-spectrometry-based lipid analysis ed. by H. Alex Brown |
| title_short | Lipidomics and bioactive lipids: Mass-spectrometry-based lipid analysis |
| title_sort | lipidomics and bioactive lipids mass spectrometry based lipid analysis |
| topic | Lipids analysis Mass Spectrometry methods Labortechnik (DE-588)4123602-6 gnd Lipidstoffwechsel (DE-588)4035875-6 gnd Massenspektrometrie (DE-588)4037882-2 gnd |
| topic_facet | Lipids analysis Mass Spectrometry methods Labortechnik Lipidstoffwechsel Massenspektrometrie |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016168960&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV000000938 |
| work_keys_str_mv | AT brownhalex lipidomicsandbioactivelipidsmassspectrometrybasedlipidanalysis |