Ultrashort laser pulses in biology and medicine:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Berlin [u.a.]
Springer
2008
|
| Schriftenreihe: | Biological and medical physics, biomedical engineering
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XV, 320 S. Ill., graph. Darst. |
| ISBN: | 9783540735656 3540735658 |
Internformat
MARC
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| 020 | |a 9783540735656 |c Gb. : ca. EUR 160.45 (freier Pr.), ca. sfr 246.00 (freier Pr.) |9 978-3-540-73565-6 | ||
| 020 | |a 3540735658 |c Gb. : ca. EUR 160.45 (freier Pr.), ca. sfr 246.00 (freier Pr.) |9 3-540-73565-8 | ||
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| 245 | 1 | 0 | |a Ultrashort laser pulses in biology and medicine |c Markus Braun ... (eds.) |
| 264 | 1 | |a Berlin [u.a.] |b Springer |c 2008 | |
| 300 | |a XV, 320 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 0 | |a Biological and medical physics, biomedical engineering | |
| 650 | 4 | |a Laser pulses, Ultrashort | |
| 650 | 4 | |a Lasers | |
| 650 | 4 | |a Pulse techniques (Electronics) | |
| 650 | 0 | 7 | |a Ultrakurzzeitlaser |0 (DE-588)4222151-1 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Medizin |0 (DE-588)4038243-6 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Biologie |0 (DE-588)4006851-1 |2 gnd |9 rswk-swf |
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| 689 | 0 | 1 | |a Biologie |0 (DE-588)4006851-1 |D s |
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| 856 | 4 | 2 | |m Digitalisierung UB Regensburg |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016154752&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-016154752 | ||
Datensatz im Suchindex
| _version_ | 1804137193256517632 |
|---|---|
| adam_text | Contents
Part I Ultrafast Lasers in Medicine
1
Ultrahigh-Resolution Optical Coherence Tomography
Using Femtosecond Lasers
J.G. Fujimoto,
A.D.
Aguirre, Y. Chen, P.R.
Herz,
P.
-L. Hsiung,
Т.Н.
Ko,
N.
Nishizawa. and F.X.
Kärtner.......................... 3
1.1
Introduction
............................................... 3
1.2
Measuring Ultrafast Optical Echoes
........................... 6
1.3
Low-Coherence
Interferometry
............................... 7
1.4
Resolution of
OCT
......................................... 8
1.5
Ultrahigh-Resolution
OCT
Using Femtosecond Lasers
........... 10
1.6
Ultrahigh-Resolution
OCT
Imaging
Using
Ti:AI2O3
Femtosecond Lasers
.......................... 11
1.7
Ultrahigh-Resolution Imaging
Using CnForsterite Femtosecond Lasers
....................... 15
1.8
Ultrahigh-Resolution
OCT
Imaging
Using Femtosecond Nd:Glass Lasers
.......................... 20
1.9
Three-Dimensional
OCT (3D-OCT)
Imaging
................... 22
1.10
Summary
.................................................. 23
References
...................................................... 24
2
Two-Photon Laser Scanning Microscopy
A.
Nimmer
jahn, P. Theer, and
F. Helmchen........................ 29
2.1
Introduction
............................................... 29
2.2
Theory and Technology
..................................... 29
2.2.1
Two-Photon Fluorescence Excitation
................... 29
2.2.2
Fluorescence Detection
................................ 33
2.2.3
Instrumentation
...................................... 35
2.2.4
Fluorescence Labeling Techniques
...................... 36
VIII Contents
2.3
Applications
............................................... 39
2.3.1
Functional Fluorescence Imaging
....................... 40
2.3.2
Photomanipulation
................................... 43
2.4
Limitations
................................................ 44
2.4.1
Spatial and Temporal Resolution
....................... 44
2.4.2
Tissue Damage
...................................... 45
2.5
Future Perspectives
......................................... 46
References
...................................................... 48
3
Femtosecond Lasers in Ophthalmology:
Surgery and Imaging
J.F.
Bille
....................................................... 53
3.1
Introduction
............................................... 53
3.2
Surgical Applications of Femtosecond Lasers in Ophthalmology.
. . 54
3.2.1
Laser-Tissue Interaction
.............................. 54
3.2.2
All-Solid-State Femtosecond Laser Technology
........... 57
3.2.3
Clinical Instrumentation
.............................. 60
3.2.4
Experimental Results
................................. 61
3.3
Imaging Applications of Femtosecond Lasers
in Ophthalmology
.......................................... 63
3.3.1
Principles of Nonlinear Microscopic Imaging
............. 63
3.3.2
Second Harmonic Generation Imaging of Collagen
Fibrils in Cornea,
Sclera,
and Optic Nerve Head
.......... 64
3.3.3
Two Photon Excited
Autofluorescence
Imaging
of Lipofuscin Granules in RPE
......................... 67
3.3.4
Aberration Free Retina Imaging
with Closed-Loop Adaptive Optics
..................... 70
3.4
Conclusion and Outlook
..................................... 71
References
...................................................... 72
Part II Ultrafast Lasers in Biology
4
Ultrafast
Peptide
and Protein Dynamics
by Vibrational Spectroscopy
P.
Hamm....................................................... 77
4.1
Introduction
............................................... 77
4.2
The Challenge of Using
IR
Spectroscopy
as Structure-Sensitive Method
............................... 78
4.3
Experimental Methods
...................................... 80
4.4
Vibrational Spectroscopy of Equilibrium Dynamics
of Peptides and Proteins
.................................... 80
4.4.1
Photon Echo Spectroscopy
............................ 81
4.4.2
2D-IR Spectroscopy
.................................. 83
Contents
IX
4.5 Vibrational
Spectroscopy of Nonequilibrium
Dynamics
of Peptides
and Proteins .................................... 86
4.6
Conclusion and
Outlook..................................... 90
References
...................................................... 91
5
Photosynthetic Light-Harvesting
T. Pullerits, T.
Polívka,
and V.
Sundström
.......................... 95
5.1
Introduction
............................................... 95
5.2
Light-Harvesting in Photosynthetic Purple Bacteria:
Energy Transfer and Trapping
............................... 96
5.2.1
B800
............................................... 97
5.2.2
Excitons
and
Polarons
in B850
......................... 98
5.2.3
Inter-Complex Excitation Transfer
......................100
5.3
Carotenoid Light-Harvesting
in the Peridinin-Chlorophyll Protein
(PCP)
...................104
5.3.1
Steady-State Spectroscopy
.............................104
5.3.2
Energy Transfer Pathways
.............................108
5.4
Carbonyl Carotenoids
in Other Light-Harvesting Systems
...........................
Ill
References
......................................................112
6
Primary Photosynthetic Energy Conversion
in Bacterial Reaction Centers
W. Zinth and J. Wachtveitl
.......................................117
6.1
Introduction
...............................................117
6.2
Structure and Absorption Spectra
of Photosynthetic Reaction Centers
...........................120
6.3
Ultrafast Reaction Steps
....................................122
6.4
Some Remarks on Superexchange Electron Transfer
.............124
6.5
Superexchange vs. Stepwise Electron Transfer
..................126
6.6
Theoretical Description of the Picosecond
ET
..................130
6.7
Experiments on Modified Reaction Centers
....................131
6.8
Optimization of Photosynthesis
..............................132
6.9
Conclusion
................................................135
References
......................................................135
7
Ultrafast Primary Reactions
in the
Photosystems
of Oxygen-Evolving Organisms
A.R. Holzwarth
..................................................141
7.1
Structural Basis of Primary Photosynthetic Reactions
...........141
7.2 Photosystem
I Structure
....................................142
7.3 Photosystem
II Structure
....................................145
7.4
Energy Transfer Processes
...................................147
7.4.1
Energy Transfer in Core Antenna/RC Particles
..........147
7.4.2
Is Energy Transfer from the Core
to the RC Rate-Limiting?
.............................147
X
Contents
7.4.3 Energy Transfer in PS
I Cores
.........................148
7.4.4 Energy Exchange
with Red
Chlorophylls in PS
I Cores
.... 149
7.4.5 Energy Transfer in PS
II Cores
........................151
7.5 Electron Transfer
Processes
..................................152
7.5.1 Photosystem
I Cores
..................................152
7.5.2 Electron Transfer in PS
II
RCs.........................154
7.6
Conclusions
................................................158
References......................................................
158
8
Primary Photochemistry in the Photoactive Yellow Protein:
The Prototype Xanthopsin
D.S.
Larsen, R. van Grondelle, and
K.J. Hellingwerf.................165
8.1
Introduction
...............................................165
8.1.1
Biological Function
...................................166
8.1.2
PYP Structure
.......................................167
8.1.3
PYP
Photocycle
.....................................169
8.2
Biophysical Techniques
......................................171
8.3
Time-Resoved Fluorescence Signals
...........................174
8.4
Electronically Resonant Transient Absorption Signals
...........176
8.4.1
Pump-Probe Measurements
...........................177
8.4.2
Pump-Dump-Probe Measurements
.....................183
8.5
Vibrationally Resonant Ultrafast Signals
......................186
8.6
Time-Resolved
Х
-Ray Diffraction Measurements
...............189
8.7
Isolated PYP Chromophores
.................................190
8.8
Quantum Calculations and Molecular Dynamics
................192
8.9
Concluding Remarks
........................................194
References
......................................................195
9
Structure Based Kinetics
by Time-Resolved X-ray Crystallography
M. Schmidt
.....................................................201
9.1
Introduction
...............................................201
9.1.1
Structure and Function of Proteins
.....................201
9.1.2
Structure Determination of Intermediate States
by Stabilization (Trapping) of their Occupation
..........203
9.2
Crystallography Meets Chemical Kinetics
......................206
9.2.1
Chemical Kinetics
....................................206
9.2.2
Time-Resolved
Х
-Ray Structure Analysis
................208
9.3
From the Reaction Initiation
to Difference Electron Density Maps
..........................213
9.3.1
Reaction Initiation
...................................213
9.3.2
Detectors
...........................................214
9.3.3
Data Reduction
......................................215
9.3.4
Difference Maps
......................................215
Contents
XI
9.4 Experiments...............................................216
9.4.1 Myoglobin...........................................216
9.4.2
The Photoactive Yellow
Protein........................218
9.5
A New Method for the Analysis of Time-Resolved X-ray Data
.. . 220
9.5.1
The Singular Value Decomposition
.....................220
9.5.2
The Noise Filter
.....................................222
9.5.3
Transient Kinetics and Kinetic Mechanisms
from the
SVD
.......................................224
9.5.4
Determination of the Structures of the Intermediates
.....226
9.5.5
Posterior Analysis
....................................227
9.5.6
Verification of the Functionality of the SVD-Driven
Analysis by Mock Data
...............................229
9.6
The
SVD
Analysis of Experimental Time-Resolved Data
........230
9.6.1
SVD-Flattening
......................................230
9.6.2
The Mechanistic Analysis of the PYP data
..............230
9.6.3
The Structures of the Intermediates
in the Late
Photocycle
Between
5цѕ
and 100ms
..........232
9.6.4
Plausible Kinetic Mechanisms
..........................232
9.6.5
The Entire
Photocycles
of the Wild-Type PYP
and its E46Q-Mutant
.................................234
9.7
Picosecond Time Resolution and Beyond
......................235
9.8
More Applications
..........................................236
References
......................................................237
10
Primary Reactions in Retinal Proteins
R. Oilier
........................................................243
10.1
Introduction
...............................................243
10.2
Systems
...................................................246
10.3
A First Glance at the Primary Reaction Dynamics
.............249
10.3.1 11-
Cis
—>
AU-
Trons Isomerization
......................250
10.3.2
All- Trans
—> 13-
Cis
Isomerization
......................252
10.4
Discussion
.................................................256
10.4.1
When Does Isomerization Occur?
......................256
10.4.2
Ultrafast Electronic Surface Crossing
...................263
10.4.3
Reaction Models
.....................................264
10.4.4
Wavepacket Dynamics after Electronic Excitation
........266
10.4.5
Chromophore-Protein Interaction
.......................268
References
......................................................271
11
Ultrashort
Laser Pulses in Single Molecule Spectroscopy
E.
Haustein
and P.
Schwüle.......................................279
11.1
Introduction
...............................................279
11.2
Basic Concepts of Fluorescence
...............................279
11.2.1
Fluorescence
.........................................279
11.2.2
Fluorescence Lifetime
.................................281
XII Contents
11.2.3
Fluorescent
Dyes
.....................................284
11.2.4
Autofluorescent
Proteins
..............................284
11.2.5
Organic Chromophores
................................284
11.2.6
Quantum Dots
.......................................285
11.3
Instrumentation and Set-up
..................................286
11.3.1
Confocal Set-up: Continuous-Wave (cw-) Excitation
......286
11.3.2
Confocal Set-up: Pulsed Excitation
.....................287
11.4
Time-Correlated Single Photon Counting (TCSPC)
.............289
11.4.1
Fluorescence Lifetime
.................................289
11.4.2
Instrument Response Function (IRF)
...................291
11.4.3
Analysis of Fluorescence Decays
........................292
11.5
Fluorescence Correlation Spectroscopy (FCS)
..................294
11.5.1
One-Photon Excitation
...............................294
11.6
Two-Photon Excitation
.....................................297
11.6.1
Correlation of Photon Arrival Times
....................298
11.7
Gated Detection
...........................................300
11.7.1
Time-Resolved Fluorescence Correlation Spectroscopy
... .301
11.8
Lifetime-Assisted Crosstalk-Suppression
for Cross-Correlation Spectroscopy
...........................302
11.9
Anisotropy
................................................302
11.9.1
Theory
.............................................302
11.9.2
Time-Resolved Fluorescence Anisotropy
.................303
11.9.3
Static Anisotropy
....................................303
11.9.4
Time-Resolved Anisotropy
.............................304
11.10
Burst -Analysis
...........................................304
11.11
Conclusions
................................................306
References
......................................................306
Index
..........................................................311
|
| adam_txt |
Contents
Part I Ultrafast Lasers in Medicine
1
Ultrahigh-Resolution Optical Coherence Tomography
Using Femtosecond Lasers
J.G. Fujimoto,
A.D.
Aguirre, Y. Chen, P.R.
Herz,
P.
-L. Hsiung,
Т.Н.
Ko,
N.
Nishizawa. and F.X.
Kärtner. 3
1.1
Introduction
. 3
1.2
Measuring Ultrafast Optical Echoes
. 6
1.3
Low-Coherence
Interferometry
. 7
1.4
Resolution of
OCT
. 8
1.5
Ultrahigh-Resolution
OCT
Using Femtosecond Lasers
. 10
1.6
Ultrahigh-Resolution
OCT
Imaging
Using
Ti:AI2O3
Femtosecond Lasers
. 11
1.7
Ultrahigh-Resolution Imaging
Using CnForsterite Femtosecond Lasers
. 15
1.8
Ultrahigh-Resolution
OCT
Imaging
Using Femtosecond Nd:Glass Lasers
. 20
1.9
Three-Dimensional
OCT (3D-OCT)
Imaging
. 22
1.10
Summary
. 23
References
. 24
2
Two-Photon Laser Scanning Microscopy
A.
Nimmer
jahn, P. Theer, and
F. Helmchen. 29
2.1
Introduction
. 29
2.2
Theory and Technology
. 29
2.2.1
Two-Photon Fluorescence Excitation
. 29
2.2.2
Fluorescence Detection
. 33
2.2.3
Instrumentation
. 35
2.2.4
Fluorescence Labeling Techniques
. 36
VIII Contents
2.3
Applications
. 39
2.3.1
Functional Fluorescence Imaging
. 40
2.3.2
Photomanipulation
. 43
2.4
Limitations
. 44
2.4.1
Spatial and Temporal Resolution
. 44
2.4.2
Tissue Damage
. 45
2.5
Future Perspectives
. 46
References
. 48
3
Femtosecond Lasers in Ophthalmology:
Surgery and Imaging
J.F.
Bille
. 53
3.1
Introduction
. 53
3.2
Surgical Applications of Femtosecond Lasers in Ophthalmology.
. . 54
3.2.1
Laser-Tissue Interaction
. 54
3.2.2
All-Solid-State Femtosecond Laser Technology
. 57
3.2.3
Clinical Instrumentation
. 60
3.2.4
Experimental Results
. 61
3.3
Imaging Applications of Femtosecond Lasers
in Ophthalmology
. 63
3.3.1
Principles of Nonlinear Microscopic Imaging
. 63
3.3.2
Second Harmonic Generation Imaging of Collagen
Fibrils in Cornea,
Sclera,
and Optic Nerve Head
. 64
3.3.3
Two Photon Excited
Autofluorescence
Imaging
of Lipofuscin Granules in RPE
. 67
3.3.4
Aberration Free Retina Imaging
with Closed-Loop Adaptive Optics
. 70
3.4
Conclusion and Outlook
. 71
References
. 72
Part II Ultrafast Lasers in Biology
4
Ultrafast
Peptide
and Protein Dynamics
by Vibrational Spectroscopy
P.
Hamm. 77
4.1
Introduction
. 77
4.2
The Challenge of Using
IR
Spectroscopy
as Structure-Sensitive Method
. 78
4.3
Experimental Methods
. 80
4.4
Vibrational Spectroscopy of Equilibrium Dynamics
of Peptides and Proteins
. 80
4.4.1
Photon Echo Spectroscopy
. 81
4.4.2
2D-IR Spectroscopy
. 83
Contents
IX
4.5 Vibrational
Spectroscopy of Nonequilibrium
Dynamics
of Peptides
and Proteins . 86
4.6
Conclusion and
Outlook. 90
References
. 91
5
Photosynthetic Light-Harvesting
T. Pullerits, T.
Polívka,
and V.
Sundström
. 95
5.1
Introduction
. 95
5.2
Light-Harvesting in Photosynthetic Purple Bacteria:
Energy Transfer and Trapping
. 96
5.2.1
B800
. 97
5.2.2
Excitons
and
Polarons
in B850
. 98
5.2.3
Inter-Complex Excitation Transfer
.100
5.3
Carotenoid Light-Harvesting
in the Peridinin-Chlorophyll Protein
(PCP)
.104
5.3.1
Steady-State Spectroscopy
.104
5.3.2
Energy Transfer Pathways
.108
5.4
Carbonyl Carotenoids
in Other Light-Harvesting Systems
.
Ill
References
.112
6
Primary Photosynthetic Energy Conversion
in Bacterial Reaction Centers
W. Zinth and J. Wachtveitl
.117
6.1
Introduction
.117
6.2
Structure and Absorption Spectra
of Photosynthetic Reaction Centers
.120
6.3
Ultrafast Reaction Steps
.122
6.4
Some Remarks on Superexchange Electron Transfer
.124
6.5
Superexchange vs. Stepwise Electron Transfer
.126
6.6
Theoretical Description of the Picosecond
ET
.130
6.7
Experiments on Modified Reaction Centers
.131
6.8
Optimization of Photosynthesis
.132
6.9
Conclusion
.135
References
.135
7
Ultrafast Primary Reactions
in the
Photosystems
of Oxygen-Evolving Organisms
A.R. Holzwarth
.141
7.1
Structural Basis of Primary Photosynthetic Reactions
.141
7.2 Photosystem
I Structure
.142
7.3 Photosystem
II Structure
.145
7.4
Energy Transfer Processes
.147
7.4.1
Energy Transfer in Core Antenna/RC Particles
.147
7.4.2
Is Energy Transfer from the Core
to the RC Rate-Limiting?
.147
X
Contents
7.4.3 Energy Transfer in PS
I Cores
.148
7.4.4 Energy Exchange
with Red
Chlorophylls in PS
I Cores
. 149
7.4.5 Energy Transfer in PS
II Cores
.151
7.5 Electron Transfer
Processes
.152
7.5.1 Photosystem
I Cores
.152
7.5.2 Electron Transfer in PS
II
RCs.154
7.6
Conclusions
.158
References.
158
8
Primary Photochemistry in the Photoactive Yellow Protein:
The Prototype Xanthopsin
D.S.
Larsen, R. van Grondelle, and
K.J. Hellingwerf.165
8.1
Introduction
.165
8.1.1
Biological Function
.166
8.1.2
PYP Structure
.167
8.1.3
PYP
Photocycle
.169
8.2
Biophysical Techniques
.171
8.3
Time-Resoved Fluorescence Signals
.174
8.4
Electronically Resonant Transient Absorption Signals
.176
8.4.1
Pump-Probe Measurements
.177
8.4.2
Pump-Dump-Probe Measurements
.183
8.5
Vibrationally Resonant Ultrafast Signals
.186
8.6
Time-Resolved
Х
-Ray Diffraction Measurements
.189
8.7
Isolated PYP Chromophores
.190
8.8
Quantum Calculations and Molecular Dynamics
.192
8.9
Concluding Remarks
.194
References
.195
9
Structure Based Kinetics
by Time-Resolved X-ray Crystallography
M. Schmidt
.201
9.1
Introduction
.201
9.1.1
Structure and Function of Proteins
.201
9.1.2
Structure Determination of Intermediate States
by Stabilization (Trapping) of their Occupation
.203
9.2
Crystallography Meets Chemical Kinetics
.206
9.2.1
Chemical Kinetics
.206
9.2.2
Time-Resolved
Х
-Ray Structure Analysis
.208
9.3
From the Reaction Initiation
to Difference Electron Density Maps
.213
9.3.1
Reaction Initiation
.213
9.3.2
Detectors
.214
9.3.3
Data Reduction
.215
9.3.4
Difference Maps
.215
Contents
XI
9.4 Experiments.216
9.4.1 Myoglobin.216
9.4.2
The Photoactive Yellow
Protein.218
9.5
A New Method for the Analysis of Time-Resolved X-ray Data
. . 220
9.5.1
The Singular Value Decomposition
.220
9.5.2
The Noise Filter
.222
9.5.3
Transient Kinetics and Kinetic Mechanisms
from the
SVD
.224
9.5.4
Determination of the Structures of the Intermediates
.226
9.5.5
Posterior Analysis
.227
9.5.6
Verification of the Functionality of the SVD-Driven
Analysis by Mock Data
.229
9.6
The
SVD
Analysis of Experimental Time-Resolved Data
.230
9.6.1
SVD-Flattening
.230
9.6.2
The Mechanistic Analysis of the PYP data
.230
9.6.3
The Structures of the Intermediates
in the Late
Photocycle
Between
5цѕ
and 100ms
.232
9.6.4
Plausible Kinetic Mechanisms
.232
9.6.5
The Entire
Photocycles
of the Wild-Type PYP
and its E46Q-Mutant
.234
9.7
Picosecond Time Resolution and Beyond
.235
9.8
More Applications
.236
References
.237
10
Primary Reactions in Retinal Proteins
R. Oilier
.243
10.1
Introduction
.243
10.2
Systems
.246
10.3
A First Glance at the Primary Reaction Dynamics
.249
10.3.1 11-
Cis
—>
AU-
Trons Isomerization
.250
10.3.2
All- Trans
—> 13-
Cis
Isomerization
.252
10.4
Discussion
.256
10.4.1
When Does Isomerization Occur?
.256
10.4.2
Ultrafast Electronic Surface Crossing
.263
10.4.3
Reaction Models
.264
10.4.4
Wavepacket Dynamics after Electronic Excitation
.266
10.4.5
Chromophore-Protein Interaction
.268
References
.271
11
Ultrashort
Laser Pulses in Single Molecule Spectroscopy
E.
Haustein
and P.
Schwüle.279
11.1
Introduction
.279
11.2
Basic Concepts of Fluorescence
.279
11.2.1
Fluorescence
.279
11.2.2
Fluorescence Lifetime
.281
XII Contents
11.2.3
Fluorescent
Dyes
.284
11.2.4
Autofluorescent
Proteins
.284
11.2.5
Organic Chromophores
.284
11.2.6
Quantum Dots
.285
11.3
Instrumentation and Set-up
.286
11.3.1
Confocal Set-up: Continuous-Wave (cw-) Excitation
.286
11.3.2
Confocal Set-up: Pulsed Excitation
.287
11.4
Time-Correlated Single Photon Counting (TCSPC)
.289
11.4.1
Fluorescence Lifetime
.289
11.4.2
Instrument Response Function (IRF)
.291
11.4.3
Analysis of Fluorescence Decays
.292
11.5
Fluorescence Correlation Spectroscopy (FCS)
.294
11.5.1
One-Photon Excitation
.294
11.6
Two-Photon Excitation
.297
11.6.1
Correlation of Photon Arrival Times
.298
11.7
Gated Detection
.300
11.7.1
Time-Resolved Fluorescence Correlation Spectroscopy
. .301
11.8
Lifetime-Assisted Crosstalk-Suppression
for Cross-Correlation Spectroscopy
.302
11.9
Anisotropy
.302
11.9.1
Theory
.302
11.9.2
Time-Resolved Fluorescence Anisotropy
.303
11.9.3
Static Anisotropy
.303
11.9.4
Time-Resolved Anisotropy
.304
11.10
"Burst"-Analysis
.304
11.11
Conclusions
.306
References
.306
Index
.311 |
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| illustrated | Illustrated |
| index_date | 2024-07-02T19:02:00Z |
| indexdate | 2024-07-09T21:08:24Z |
| institution | BVB |
| isbn | 9783540735656 3540735658 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-016154752 |
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| physical | XV, 320 S. Ill., graph. Darst. |
| publishDate | 2008 |
| publishDateSearch | 2008 |
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| publisher | Springer |
| record_format | marc |
| series2 | Biological and medical physics, biomedical engineering |
| spelling | Ultrashort laser pulses in biology and medicine Markus Braun ... (eds.) Berlin [u.a.] Springer 2008 XV, 320 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Biological and medical physics, biomedical engineering Laser pulses, Ultrashort Lasers Pulse techniques (Electronics) Ultrakurzzeitlaser (DE-588)4222151-1 gnd rswk-swf Medizin (DE-588)4038243-6 gnd rswk-swf Biologie (DE-588)4006851-1 gnd rswk-swf Ultrakurzzeitlaser (DE-588)4222151-1 s Biologie (DE-588)4006851-1 s DE-604 Medizin (DE-588)4038243-6 s Braun, Markus Sonstige oth Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016154752&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Ultrashort laser pulses in biology and medicine Laser pulses, Ultrashort Lasers Pulse techniques (Electronics) Ultrakurzzeitlaser (DE-588)4222151-1 gnd Medizin (DE-588)4038243-6 gnd Biologie (DE-588)4006851-1 gnd |
| subject_GND | (DE-588)4222151-1 (DE-588)4038243-6 (DE-588)4006851-1 |
| title | Ultrashort laser pulses in biology and medicine |
| title_auth | Ultrashort laser pulses in biology and medicine |
| title_exact_search | Ultrashort laser pulses in biology and medicine |
| title_exact_search_txtP | Ultrashort laser pulses in biology and medicine |
| title_full | Ultrashort laser pulses in biology and medicine Markus Braun ... (eds.) |
| title_fullStr | Ultrashort laser pulses in biology and medicine Markus Braun ... (eds.) |
| title_full_unstemmed | Ultrashort laser pulses in biology and medicine Markus Braun ... (eds.) |
| title_short | Ultrashort laser pulses in biology and medicine |
| title_sort | ultrashort laser pulses in biology and medicine |
| topic | Laser pulses, Ultrashort Lasers Pulse techniques (Electronics) Ultrakurzzeitlaser (DE-588)4222151-1 gnd Medizin (DE-588)4038243-6 gnd Biologie (DE-588)4006851-1 gnd |
| topic_facet | Laser pulses, Ultrashort Lasers Pulse techniques (Electronics) Ultrakurzzeitlaser Medizin Biologie |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016154752&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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