Lipidomics and bioactive lipids: Lipids and cell signaling:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Amsterdam [u.a.]
Elsevier, Acad. Press
2007
|
| Schriftenreihe: | Methods in enzymology
434 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XLIV, 342 S. Ill., graph. Darst. |
| ISBN: | 9780123739650 0123739659 |
Internformat
MARC
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| 245 | 1 | 0 | |a Lipidomics and bioactive lipids: Lipids and cell signaling |c ed. by H. Alex Brown |
| 264 | 1 | |a Amsterdam [u.a.] |b Elsevier, Acad. Press |c 2007 | |
| 300 | |a XLIV, 342 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in enzymology |v 434 | |
| 650 | 4 | |a Lipids |x Analysis | |
| 650 | 4 | |a Lipids |x Spectra | |
| 650 | 4 | |a Lipids |x analysis | |
| 650 | 4 | |a Mass Spectrometry |x methods | |
| 650 | 4 | |a Mass spectrometry | |
| 650 | 0 | 7 | |a Lipide |0 (DE-588)4035873-2 |2 gnd |9 rswk-swf |
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| 700 | 1 | |a Brown, H. Alex |e Sonstige |4 oth | |
| 830 | 0 | |a Methods in enzymology |v 434 |w (DE-604)BV000000938 |9 434 | |
| 856 | 4 | 2 | |m Digitalisierung UB Regensburg |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016153898&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-016153898 | ||
Datensatz im Suchindex
| _version_ | 1804137191972012032 |
|---|---|
| adam_text | Contents
Contributors
xi
Preface
xvii
Volumes in Series
xix
ι.
Phospholipase Aa Assays Using
a
Radiolabeled
Substrate
and Mass Spectrometry
ι
Reí
Morikawa, Masafumi Tsujimoto, Hiroyuki
Arai,
and )unken Aoki
ι.
Introduction
2
2.
Types of
PIA
2
3.
Conventional
PLA!
Assay Using
Radiolabeled
Substrates
3
4.
Novel
PIA
Assay Using ESI-MS
6
5.
Perspective
11
Acknowledgments
11
References
11
2.
Real-Time Cell Assays of Phospholipase Aas Using
Fluorogenic Phospholipids
15
Debasis Manna and Wonhwa Cho
1.
Introduction
16
2.
Fluorogenic PLA2 Substrates
18
3.
Measuring Cellular sPLA2 Activity Using PED6 and
Red-PEDö 23
4.
Measuring Cellular cPU^a Activity Using DAPC
24
References
26
3.
Analysis and Pharmacological Targeting of
Phospholipase
С
β
Interactions with
G
Proteins
29
David M.
Lehmann,
Chujun Yuan, and Alan
V. Smreka
1.
Introduction
30
2.
Methods
31
3.
Concluding Remarks
46
Acknowledgment
47
References
47
vi
Contents
4.
Biochemical Analysis of Phospholipase
D
49
H.
Alex Brown, Lee
G. Henage,
Anita
M. Preininger,
Yun Xiang, and John H. Exton
1.
Introduction
50
2.
Assay of
Recombinant
PLD In
Vitro
52
3.
Regulated PLDi Activity
59
4.
Preparation of Activators of PLDi
60
5.
Effects of Activators on PLDi Activity
61
6.
Synergy Between PLDi Activators
62
7.
Binding of PLDi to Phospholipid Vesicles
63
8.
Kinetic Parameters of PLDi Catalytic Activity
65
9.
Kinetic Analyses of Synergistic Responses
69
10.
Phosphatidylinositol 4,5-Bisphosphate is an Essential
PLDi Activator
69
11.
In Vivo
PLD
Assay Using
Radioisotopes 74
12.
In Vivo
PLD
Assay Using Deuterated l-Butanol
74
13.
Fluorescent In Vitro
PLD
Assay
77
14.
Real-Time Diacylglycerol
Lipase
Assay
80
Acknowledgments
85
References
85
5.
Measurement of Autotaxin/Lysophospholipase
D
Activity
89
Andrew
).
Morris and Susan S. Smyth
1.
Introduction
90
2.
Overview of Methods for Determination of Autotaxin/LysoPLD Activity
93
3.
Expression of Vs-Tagged Autotaxin/LysoPLD in HEK293 Cells
94
4.
Measurement of Autotaxin/LysoPLD Activity Using
Radiolabeled
Substrates
95
5.
Measurement of Autotaxin/LysoPLD Activity Using
Fluorogenic Substrates
98
6.
Concluding Comments
100
Acknowledgment
102
References
102
6.
Platelet-Activating Factor
105
John S. Owen, Michael J. Thomas, and Robert L. Wykle
1.
Introduction
105
2.
Procedure
107
Acknowledgments
115
References
115
Contents
vii
7. Quantitative
Measurement of Phosphatidylinositol
3,4,5-trisphosphate
117
Hervé Guillou,
Len R.
Stephens, and Phillip T. Hawkins
1.
Introduction
118
2.
Measuring Levels of Radioactively Labeled
Phosphoinositides in Isolated Cells
120
3.
Measuring Ptdlns(3,4,5)P3 by Protein-Lipid Overlay
122
4.
Conclusions
126
Acknowledgments
128
References
128
8.
Measuring Phosphorylated
Akt
and Other Phosphoinositide
3-kinase-Regulated Phosphoproteins in Primary Lymphocytes
131
Amber
С
Donahue, Michael G. Kharas, and David A. Fruman
1.
Overview
132
2.
Choosing a Downstream Readout: General Considerations
134
3.
Protocols for Detection of
РІзК
-Regulated
Phosphoproteins by Immunoblot
137
4.
Protocols for Detection of Phosphoproteins by Flow Cytometry
142
5.
Discussion
147
Acknowledgments
150
References
150
9.
Regulation of Phosphatidylinositol 4-Phosphate 5-kinase
Activity by Partner Proteins
155
Yasunori Kanaho, Kazuhisa Nakayama, Michael A. Frohman,
and Takeaki Yokozeki
1.
Introduction
156
2.
Protocols
158
Acknowledgments
166
References
166
10.
Biochemical Analysis of Inositol Phosphate Kinases
171
James
С
Otto, Sashidhar Mulugu, Peter
C. Fridy,
Shean-Tai
Chiou,
Blaine
N. Armbruster,
Anthony
A. Ribeiro,
and John D. York
1.
Introduction
172
2.
Experimental Methods
174
3.
Conclusions
182
Acknowledgments
183
References
183
viii Contents
11.
Analysis of Phosphoinositides and Their Aqueous Metabolites
187
Christopher P. Berrie,
Cristiano lurisci,
Enza Piccolo,
Renzo Bagnati,
and
Daniela
Corda
1.
Introduction
188
2.
Cell Sample Extraction
191
3.
Lipid
Phase:
TLC, HPLC
Separation, and Desalting
197
4.
Aqueous Phase: HPLC Separation, Desalting, and Ascintillant Extraction
203
5.
Chemical Identification
■ 212
6.
ESI-MS/MS Identification
214
7.
Standards
219
8.
Final Considerations
226
Acknowledgments
227
References
227
12.
Combination of C17 Sphingoid Base
Homologues
and
Mass Spectroinetry Analysis as a New Approach to
Study Sphingolipid Metabolism
233
Stefka
Spassieva,
Jacek
Bielawski,
Viviana
Anelli,
and
Lina
M. Obeid
1.
Introduction
234
2.
Mass Spectrometry Analysis
235
3.
Ceramide Synthase
236
4.
In Vitro Ceramide Synthase Method
236
5.
Sphingosine Kinase
237
6.
In Vitro Sphingosine Kinase Method
238
7.
In Cells Labeling with C17 Sphingoid Base
239
Acknowledgments
240
References
240
13.
Measurement of Mammalian Sphingosine-i-Phosphate
Phosphohydrolase Activity In Vitro and In Vivo
243
Michael Maceyka, Sheldon Milstien, and Sarah Spiegel
a. Introduction
244
2.
Principle
249
3.
Measurement of SPP Activity in Cell Lysates
249
4.
Measurement of SPP Activity in Live Cells
252
Acknowledgments
253
References
253
14.
A Rapid and Sensitive Method to Measure Secretion of
Sphingosine-i-Phosphate
257
Poulami
Mitra,
Shawn G. Payne, Sheldon Milstien, and Sarah Spiegel
1.
Introduction
258
2.
Measurement of SiP
259
Contents ix
3.
Conclusions
and Perspectives
262
Acknowledgments
263
References
263
15.
Ceramide Kinase and Ceramide-i-Phosphate
265
Dayanjan S. Wijesinghe,
Nadia F. Lamour,
Antonio Gomez-Munoz, and Charles E. Chalfant
1.
Introduction
266
2.
Recombinant
Expression and Kinetic Analysis of CERK
269
3.
In Vitro Kinetic Analysis of CERK Activity Using Mixed Micellar Assays
272
4.
Effective Delivery of CiP to Cells in Tissue Culture to
Study Biological Effects
278
5.
Analysis of Levels of Kinase-Derived CaP in Cells
281
6.
Analysis of CERK Localization in Cells
284
7.
Analysis of CERK Function by siRNA-Mediated Manipulation of
CERK Expression
286
8.
Analysis of CERK mRNA Levels by Q-PCR
288
Acknowledgments
289
References
290
16.
Measurement of Mammalian Diacylglycerol Kinase Activity
In Vitro and in Cells
293
Richard M. Epand and Matthew K. Topham
1.
Introduction
294
2.
In Vitro Assay of DGK
295
3.
Measuring DGK Activity in Subcellular Compartments
300
4.
Measuring DGK Activity in Cultured Cells
301
5.
Summary
303
References
303
17.
Lipid
Phosphate Phosphatases from Saccharomyces
cerev isìae
305
George M. Carman and Wen-I Wu
1.
Introduction
306
2.
Preparation of
Radiolabeled
Substrates
307
3.
Assay Methods
307
4.
Growth of Yeast
308
5.
Purification Procedure
308
6.
Properties of DPPi- and LPPi-Encoded
Lipid
Phosphate Phosphatases
311
Acknowledgment
313
References
313
Author Index
317
Subject Index
335
|
| adam_txt |
Contents
Contributors
xi
Preface
xvii
Volumes in Series
xix
ι.
Phospholipase Aa Assays Using
a
Radiolabeled
Substrate
and Mass Spectrometry
ι
Reí
Morikawa, Masafumi Tsujimoto, Hiroyuki
Arai,
and )unken Aoki
ι.
Introduction
2
2.
Types of
PIA
2
3.
Conventional
PLA!
Assay Using
Radiolabeled
Substrates
3
4.
Novel
PIA
Assay Using ESI-MS
6
5.
Perspective
11
Acknowledgments
11
References
11
2.
Real-Time Cell Assays of Phospholipase Aas Using
Fluorogenic Phospholipids
15
Debasis Manna and Wonhwa Cho
1.
Introduction
16
2.
Fluorogenic PLA2 Substrates
18
3.
Measuring Cellular sPLA2 Activity Using PED6 and
Red-PEDö 23
4.
Measuring Cellular cPU^a Activity Using DAPC
24
References
26
3.
Analysis and Pharmacological Targeting of
Phospholipase
С
β
Interactions with
G
Proteins
29
David M.
Lehmann,
Chujun Yuan, and Alan
V. Smreka
1.
Introduction
30
2.
Methods
31
3.
Concluding Remarks
46
Acknowledgment
47
References
47
vi
Contents
4.
Biochemical Analysis of Phospholipase
D
49
H.
Alex Brown, Lee
G. Henage,
Anita
M. Preininger,
Yun Xiang, and John H. Exton
1.
Introduction
50
2.
Assay of
Recombinant
PLD In
Vitro
52
3.
Regulated PLDi Activity
59
4.
Preparation of Activators of PLDi
60
5.
Effects of Activators on PLDi Activity
61
6.
Synergy Between PLDi Activators
62
7.
Binding of PLDi to Phospholipid Vesicles
63
8.
Kinetic Parameters of PLDi Catalytic Activity
65
9.
Kinetic Analyses of Synergistic Responses
69
10.
Phosphatidylinositol 4,5-Bisphosphate is an Essential
PLDi Activator
69
11.
In Vivo
PLD
Assay Using
Radioisotopes 74
12.
In Vivo
PLD
Assay Using Deuterated l-Butanol
74
13.
Fluorescent In Vitro
PLD
Assay
77
14.
Real-Time Diacylglycerol
Lipase
Assay
80
Acknowledgments
85
References
85
5.
Measurement of Autotaxin/Lysophospholipase
D
Activity
89
Andrew
).
Morris and Susan S. Smyth
1.
Introduction
90
2.
Overview of Methods for Determination of Autotaxin/LysoPLD Activity
93
3.
Expression of Vs-Tagged Autotaxin/LysoPLD in HEK293 Cells
94
4.
Measurement of Autotaxin/LysoPLD Activity Using
Radiolabeled
Substrates
95
5.
Measurement of Autotaxin/LysoPLD Activity Using
Fluorogenic Substrates
98
6.
Concluding Comments
100
Acknowledgment
102
References
102
6.
Platelet-Activating Factor
105
John S. Owen, Michael J. Thomas, and Robert L. Wykle
1.
Introduction
105
2.
Procedure
107
Acknowledgments
115
References
115
Contents
vii
7. Quantitative
Measurement of Phosphatidylinositol
3,4,5-trisphosphate
117
Hervé Guillou,
Len R.
Stephens, and Phillip T. Hawkins
1.
Introduction
118
2.
Measuring Levels of Radioactively Labeled
Phosphoinositides in Isolated Cells
120
3.
Measuring Ptdlns(3,4,5)P3 by Protein-Lipid Overlay
122
4.
Conclusions
126
Acknowledgments
128
References
128
8.
Measuring Phosphorylated
Akt
and Other Phosphoinositide
3-kinase-Regulated Phosphoproteins in Primary Lymphocytes
131
Amber
С
Donahue, Michael G. Kharas, and David A. Fruman
1.
Overview
132
2.
Choosing a Downstream Readout: General Considerations
134
3.
Protocols for Detection of
РІзК
-Regulated
Phosphoproteins by Immunoblot
137
4.
Protocols for Detection of Phosphoproteins by Flow Cytometry
142
5.
Discussion
147
Acknowledgments
150
References
150
9.
Regulation of Phosphatidylinositol 4-Phosphate 5-kinase
Activity by Partner Proteins
155
Yasunori Kanaho, Kazuhisa Nakayama, Michael A. Frohman,
and Takeaki Yokozeki
1.
Introduction
156
2.
Protocols
158
Acknowledgments
166
References
166
10.
Biochemical Analysis of Inositol Phosphate Kinases
171
James
С
Otto, Sashidhar Mulugu, Peter
C. Fridy,
Shean-Tai
Chiou,
Blaine
N. Armbruster,
Anthony
A. Ribeiro,
and John D. York
1.
Introduction
172
2.
Experimental Methods
174
3.
Conclusions
182
Acknowledgments
183
References
183
viii Contents
11.
Analysis of Phosphoinositides and Their Aqueous Metabolites
187
Christopher P. Berrie,
Cristiano lurisci,
Enza Piccolo,
Renzo Bagnati,
and
Daniela
Corda
1.
Introduction
188
2.
Cell Sample Extraction
191
3.
Lipid
Phase:
TLC, HPLC
Separation, and Desalting
197
4.
Aqueous Phase: HPLC Separation, Desalting, and Ascintillant Extraction
203
5.
Chemical Identification
■ 212
6.
ESI-MS/MS Identification
214
7.
Standards
219
8.
Final Considerations
226
Acknowledgments
227
References
227
12.
Combination of C17 Sphingoid Base
Homologues
and
Mass Spectroinetry Analysis as a New Approach to
Study Sphingolipid Metabolism
233
Stefka
Spassieva,
Jacek
Bielawski,
Viviana
Anelli,
and
Lina
M. Obeid
1.
Introduction
234
2.
Mass Spectrometry Analysis
235
3.
Ceramide Synthase
236
4.
In Vitro Ceramide Synthase Method
236
5.
Sphingosine Kinase
237
6.
In Vitro Sphingosine Kinase Method
238
7.
In Cells Labeling with C17 Sphingoid Base
239
Acknowledgments
240
References
240
13.
Measurement of Mammalian Sphingosine-i-Phosphate
Phosphohydrolase Activity In Vitro and In Vivo
243
Michael Maceyka, Sheldon Milstien, and Sarah Spiegel
a. Introduction
244
2.
Principle
249
3.
Measurement of SPP Activity in Cell Lysates
249
4.
Measurement of SPP Activity in Live Cells
252
Acknowledgments
253
References
253
14.
A Rapid and Sensitive Method to Measure Secretion of
Sphingosine-i-Phosphate
257
Poulami
Mitra,
Shawn G. Payne, Sheldon Milstien, and Sarah Spiegel
1.
Introduction
258
2.
Measurement of SiP
259
Contents ix
3.
Conclusions
and Perspectives
262
Acknowledgments
263
References
263
15.
Ceramide Kinase and Ceramide-i-Phosphate
265
Dayanjan S. Wijesinghe,
Nadia F. Lamour,
Antonio Gomez-Munoz, and Charles E. Chalfant
1.
Introduction
266
2.
Recombinant
Expression and Kinetic Analysis of CERK
269
3.
In Vitro Kinetic Analysis of CERK Activity Using Mixed Micellar Assays
272
4.
Effective Delivery of CiP to Cells in Tissue Culture to
Study Biological Effects
278
5.
Analysis of Levels of Kinase-Derived CaP in Cells
281
6.
Analysis of CERK Localization in Cells
284
7.
Analysis of CERK Function by siRNA-Mediated Manipulation of
CERK Expression
286
8.
Analysis of CERK mRNA Levels by Q-PCR
288
Acknowledgments
289
References
290
16.
Measurement of Mammalian Diacylglycerol Kinase Activity
In Vitro and in Cells
293
Richard M. Epand and Matthew K. Topham
1.
Introduction
294
2.
In Vitro Assay of DGK
295
3.
Measuring DGK Activity in Subcellular Compartments
300
4.
Measuring DGK Activity in Cultured Cells
301
5.
Summary
303
References
303
17.
Lipid
Phosphate Phosphatases from Saccharomyces
cerev'isìae
305
George M. Carman and Wen-I Wu
1.
Introduction
306
2.
Preparation of
Radiolabeled
Substrates
307
3.
Assay Methods
307
4.
Growth of Yeast
308
5.
Purification Procedure
308
6.
Properties of DPPi- and LPPi-Encoded
Lipid
Phosphate Phosphatases
311
Acknowledgment
313
References
313
Author Index
317
Subject Index
335 |
| any_adam_object | 1 |
| any_adam_object_boolean | 1 |
| building | Verbundindex |
| bvnumber | BV022949405 |
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| callnumber-search | QP601 |
| callnumber-sort | QP 3601 |
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| classification_rvk | WC 4355 |
| classification_tum | CHE 840f |
| ctrlnum | (OCoLC)181350481 (DE-599)BVBBV022949405 |
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| dewey-ones | 547 - Organic chemistry |
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| dewey-search | 547.77046 |
| dewey-sort | 3547.77046 |
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| discipline_str_mv | Chemie / Pharmazie Biologie Chemie |
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| genre | (DE-588)4143413-4 Aufsatzsammlung gnd-content |
| genre_facet | Aufsatzsammlung |
| id | DE-604.BV022949405 |
| illustrated | Illustrated |
| index_date | 2024-07-02T19:01:30Z |
| indexdate | 2024-07-09T21:08:23Z |
| institution | BVB |
| isbn | 9780123739650 0123739659 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-016153898 |
| oclc_num | 181350481 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-12 DE-91G DE-BY-TUM DE-20 DE-19 DE-BY-UBM |
| owner_facet | DE-355 DE-BY-UBR DE-12 DE-91G DE-BY-TUM DE-20 DE-19 DE-BY-UBM |
| physical | XLIV, 342 S. Ill., graph. Darst. |
| publishDate | 2007 |
| publishDateSearch | 2007 |
| publishDateSort | 2007 |
| publisher | Elsevier, Acad. Press |
| record_format | marc |
| series | Methods in enzymology |
| series2 | Methods in enzymology |
| spelling | Lipidomics and bioactive lipids: Lipids and cell signaling ed. by H. Alex Brown Amsterdam [u.a.] Elsevier, Acad. Press 2007 XLIV, 342 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in enzymology 434 Lipids Analysis Lipids Spectra Lipids analysis Mass Spectrometry methods Mass spectrometry Lipide (DE-588)4035873-2 gnd rswk-swf Labortechnik (DE-588)4123602-6 gnd rswk-swf Signaltransduktion (DE-588)4318717-1 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Lipide (DE-588)4035873-2 s Signaltransduktion (DE-588)4318717-1 s Labortechnik (DE-588)4123602-6 s b DE-604 Brown, H. Alex Sonstige oth Methods in enzymology 434 (DE-604)BV000000938 434 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016153898&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Lipidomics and bioactive lipids: Lipids and cell signaling Methods in enzymology Lipids Analysis Lipids Spectra Lipids analysis Mass Spectrometry methods Mass spectrometry Lipide (DE-588)4035873-2 gnd Labortechnik (DE-588)4123602-6 gnd Signaltransduktion (DE-588)4318717-1 gnd |
| subject_GND | (DE-588)4035873-2 (DE-588)4123602-6 (DE-588)4318717-1 (DE-588)4143413-4 |
| title | Lipidomics and bioactive lipids: Lipids and cell signaling |
| title_auth | Lipidomics and bioactive lipids: Lipids and cell signaling |
| title_exact_search | Lipidomics and bioactive lipids: Lipids and cell signaling |
| title_exact_search_txtP | Lipidomics and bioactive lipids: Lipids and cell signaling |
| title_full | Lipidomics and bioactive lipids: Lipids and cell signaling ed. by H. Alex Brown |
| title_fullStr | Lipidomics and bioactive lipids: Lipids and cell signaling ed. by H. Alex Brown |
| title_full_unstemmed | Lipidomics and bioactive lipids: Lipids and cell signaling ed. by H. Alex Brown |
| title_short | Lipidomics and bioactive lipids: Lipids and cell signaling |
| title_sort | lipidomics and bioactive lipids lipids and cell signaling |
| topic | Lipids Analysis Lipids Spectra Lipids analysis Mass Spectrometry methods Mass spectrometry Lipide (DE-588)4035873-2 gnd Labortechnik (DE-588)4123602-6 gnd Signaltransduktion (DE-588)4318717-1 gnd |
| topic_facet | Lipids Analysis Lipids Spectra Lipids analysis Mass Spectrometry methods Mass spectrometry Lipide Labortechnik Signaltransduktion Aufsatzsammlung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016153898&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV000000938 |
| work_keys_str_mv | AT brownhalex lipidomicsandbioactivelipidslipidsandcellsignaling |