From genes to genomes: concepts and applications of DNA technology
Gespeichert in:
| Hauptverfasser: | , |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Chichester [u.a.]
Wiley
2007
|
| Ausgabe: | 2. ed. |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | X, 384 S. Ill., graph. Darst. |
| ISBN: | 9780470017340 9780470017333 |
Internformat
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| 100 | 1 | |a Dale, Jeremy W. |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a From genes to genomes |b concepts and applications of DNA technology |c Jeremy W. Dale and Malcolm von Schantz |
| 250 | |a 2. ed. | ||
| 264 | 1 | |a Chichester [u.a.] |b Wiley |c 2007 | |
| 300 | |a X, 384 S. |b Ill., graph. Darst. | ||
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| 650 | 4 | |a DNA, Recombinant | |
| 650 | 4 | |a Genes | |
| 650 | 4 | |a Genetic Engineering | |
| 650 | 4 | |a Genetic engineering | |
| 650 | 4 | |a Genome | |
| 650 | 4 | |a Genomes | |
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Datensatz im Suchindex
| _version_ | 1804137186883272704 |
|---|---|
| adam_text | Contents
Preface ix
1 Introduction 1
2 Basic molecular biology 7
2.1 Nucleic acid structure 7
2.2 What is a gene? 15
2.3 Information flow: gene expression 16
2.4 Gene structure and organization 21
3 How to clone a gene 25
3.1 What is cloning? 25
3.2 Overview of the procedures 26
3.3 Gene libraries 29
3.4 Hybridization 30
3.5 Polymerase chain reaction 32
3.6 Extraction and purification of nucleic acids 33
3.7 Detection and quantitation of nucleic acids 36
3.8 Gel electrophoresis 37
4 Cutting and joining DNA 41
4.1 Restriction endojnucleases 41
4.2 Ligation. • /.. 47
4.3 Modification of restriction fragment ends 53
4.4 Other ways of joining DNA molecules 57
5 Vectors 61
5.1 Plasmid vectors 61
5.2 Vectors based on the lambda bacteriophage 69
5.3 Cosmids 78
5.4 M13 vectors 79
5.5 Expression vectors 81
5.6 Vectors for cloning and expression in eukaryotic cells 84
5.7 Supervectors: YACs and BACs 92
5.8 Summary 94
6 Genomic and cDNA libraries 95
6.1 Genomic libraries 96
6.2 Growing and storing libraries 105
6.3 cDNA libraries 106
6.4 Random, arrayed and ordered libraries 113
7 Finding the right clone 117
7.1 Screening libraries with gene probes 117
7.2 Screening expression libraries with antibodies 128
7.3 Subcloning 130
7.4 Characterization of plasmid clones 131
8 Polymerase chain reaction 135
8.1 The PCR reaction 136
8.2 PCR in practice 140
8.3 Cloning PCR products 144
8.4 Long range PCR 146
8.5 Reverse transcription PCR 146
8.6 Rapid amplification of cDNA ends 147
8.7 Quantitative PCR 149
8.8 Applications of PCR 154
9 Characterization of a cloned gene 159
9.1 DNA sequencing 159
9.2 Databank entries and annotation 167
9.3 Sequence analysis 173
9.4 Sequence comparisons 178
9.5 Protein structure 190
9.6 Confirming gene function 196
10 Analysis of gene expression 201
10.1 Analysing transcription 201
10.2 Methods for studying the promoter 209
10.3 Regulatory elements and DNA binding proteins 213
10.4 Translational analysis 217
11 Products from native and manipulated cloned genes 221
11.1 Factors affecting expression of cloned genes 222
11.2 Expression of cloned genes in bacteria 227
11.3 Expression in eukaryotic host cells 235
11.4 Adding tags and signals 239
11.5 In vitro mutagenesis 242
11.6 Vaccines 247
12 Genomic analysis 251
12.1 Genome sequencing 251
12.2 Analysis and annotation 261
12.3 Comparing genomes 269
12.4 Genome browsers 271
12.5 Relating genes and functions: genetic and physical maps 273
12.6 Transposon mutagenesis and other screening techniques 276
12.7 Conclusion 285
13 Analysis of genetic variation 287
13.1 Single nucleotide polymorphisms 288
13.2 Larger scale variations 291
13.3 Other methods for studying variation 293
13.4 Human genetic diseases 300
13.5 Molecular phylogeny 305
14 Post genomic analysis 313
14.1 Analysing transcription; transcriptomes 313
14.2 Array based methods 319
14.3 Translational analysis; proteomics 326
14.4 Post translational analysis: protein interactions 329
14.5 Integrative studies; systems biology 332
15 Modifying organisms; transgenics 333
15.1 Modification of bacteria and viruses: live vaccines 333
15.2 Transgenesis and cloning 337
15.3 Animal transgenesis 338
15.4 Applications of transgenic animals 347
15.5 Transgenic plants and their applications 351
Glossary 355
Bibliography 373
Index 375
|
| adam_txt |
Contents
Preface ix
1 Introduction 1
2 Basic molecular biology 7
2.1 Nucleic acid structure 7
2.2 What is a gene? 15
2.3 Information flow: gene expression 16
2.4 Gene structure and organization 21
3 How to clone a gene 25
3.1 What is cloning? 25
3.2 Overview of the procedures 26
3.3 Gene libraries 29
3.4 Hybridization 30
3.5 Polymerase chain reaction 32
3.6 Extraction and purification of nucleic acids 33
3.7 Detection and quantitation of nucleic acids 36
3.8 Gel electrophoresis 37
4 Cutting and joining DNA 41
4.1 Restriction endojnucleases 41
4.2 Ligation. • /. 47
4.3 Modification of restriction fragment ends 53
4.4 Other ways of joining DNA molecules 57
5 Vectors 61
5.1 Plasmid vectors 61
5.2 Vectors based on the lambda bacteriophage 69
5.3 Cosmids 78
5.4 M13 vectors 79
5.5 Expression vectors 81
5.6 Vectors for cloning and expression in eukaryotic cells 84
5.7 Supervectors: YACs and BACs 92
5.8 Summary 94
6 Genomic and cDNA libraries 95
6.1 Genomic libraries 96
6.2 Growing and storing libraries 105
6.3 cDNA libraries 106
6.4 Random, arrayed and ordered libraries 113
7 Finding the right clone 117
7.1 Screening libraries with gene probes 117
7.2 Screening expression libraries with antibodies 128
7.3 Subcloning 130
7.4 Characterization of plasmid clones 131
8 Polymerase chain reaction 135
8.1 The PCR reaction 136
8.2 PCR in practice 140
8.3 Cloning PCR products 144
8.4 Long range PCR 146
8.5 Reverse transcription PCR 146
8.6 Rapid amplification of cDNA ends 147
8.7 Quantitative PCR 149
8.8 Applications of PCR 154
9 Characterization of a cloned gene 159
9.1 DNA sequencing 159
9.2 Databank entries and annotation 167
9.3 Sequence analysis 173
9.4 Sequence comparisons 178
9.5 Protein structure 190
9.6 Confirming gene function 196
10 Analysis of gene expression 201
10.1 Analysing transcription 201
10.2 Methods for studying the promoter 209
10.3 Regulatory elements and DNA binding proteins 213
10.4 Translational analysis 217
11 Products from native and manipulated cloned genes 221
11.1 Factors affecting expression of cloned genes 222
11.2 Expression of cloned genes in bacteria 227
11.3 Expression in eukaryotic host cells 235
11.4 Adding tags and signals 239
11.5 In vitro mutagenesis 242
11.6 Vaccines 247
12 Genomic analysis 251
12.1 Genome sequencing 251
12.2 Analysis and annotation 261
12.3 Comparing genomes 269
12.4 Genome browsers 271
12.5 Relating genes and functions: genetic and physical maps 273
12.6 Transposon mutagenesis and other screening techniques 276
12.7 Conclusion 285
13 Analysis of genetic variation 287
13.1 Single nucleotide polymorphisms 288
13.2 Larger scale variations 291
13.3 Other methods for studying variation 293
13.4 Human genetic diseases 300
13.5 Molecular phylogeny 305
14 Post genomic analysis 313
14.1 Analysing transcription; transcriptomes 313
14.2 Array based methods 319
14.3 Translational analysis; proteomics 326
14.4 Post translational analysis: protein interactions 329
14.5 Integrative studies; systems biology 332
15 Modifying organisms; transgenics 333
15.1 Modification of bacteria and viruses: live vaccines 333
15.2 Transgenesis and cloning 337
15.3 Animal transgenesis 338
15.4 Applications of transgenic animals 347
15.5 Transgenic plants and their applications 351
Glossary 355
Bibliography 373
Index 375 |
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| index_date | 2024-07-02T18:59:56Z |
| indexdate | 2024-07-09T21:08:18Z |
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| isbn | 9780470017340 9780470017333 |
| language | English |
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| spelling | Dale, Jeremy W. Verfasser aut From genes to genomes concepts and applications of DNA technology Jeremy W. Dale and Malcolm von Schantz 2. ed. Chichester [u.a.] Wiley 2007 X, 384 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier ADN Gènes Génie génétique Génomes Ingeniería genética Cloning, Molecular DNA DNA, Recombinant Genes Genetic Engineering Genetic engineering Genome Genomes Molekulargenetik (DE-588)4039987-4 gnd rswk-swf DNS (DE-588)4070512-2 gnd rswk-swf Gentechnologie (DE-588)4071722-7 gnd rswk-swf DNS (DE-588)4070512-2 s Gentechnologie (DE-588)4071722-7 s 1\p DE-604 Molekulargenetik (DE-588)4039987-4 s 2\p DE-604 Schantz, Malcolm von 1966- Verfasser (DE-588)140576053 aut HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016150952&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk 2\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Dale, Jeremy W. Schantz, Malcolm von 1966- From genes to genomes concepts and applications of DNA technology ADN Gènes Génie génétique Génomes Ingeniería genética Cloning, Molecular DNA DNA, Recombinant Genes Genetic Engineering Genetic engineering Genome Genomes Molekulargenetik (DE-588)4039987-4 gnd DNS (DE-588)4070512-2 gnd Gentechnologie (DE-588)4071722-7 gnd |
| subject_GND | (DE-588)4039987-4 (DE-588)4070512-2 (DE-588)4071722-7 |
| title | From genes to genomes concepts and applications of DNA technology |
| title_auth | From genes to genomes concepts and applications of DNA technology |
| title_exact_search | From genes to genomes concepts and applications of DNA technology |
| title_exact_search_txtP | From genes to genomes concepts and applications of DNA technology |
| title_full | From genes to genomes concepts and applications of DNA technology Jeremy W. Dale and Malcolm von Schantz |
| title_fullStr | From genes to genomes concepts and applications of DNA technology Jeremy W. Dale and Malcolm von Schantz |
| title_full_unstemmed | From genes to genomes concepts and applications of DNA technology Jeremy W. Dale and Malcolm von Schantz |
| title_short | From genes to genomes |
| title_sort | from genes to genomes concepts and applications of dna technology |
| title_sub | concepts and applications of DNA technology |
| topic | ADN Gènes Génie génétique Génomes Ingeniería genética Cloning, Molecular DNA DNA, Recombinant Genes Genetic Engineering Genetic engineering Genome Genomes Molekulargenetik (DE-588)4039987-4 gnd DNS (DE-588)4070512-2 gnd Gentechnologie (DE-588)4071722-7 gnd |
| topic_facet | ADN Gènes Génie génétique Génomes Ingeniería genética Cloning, Molecular DNA DNA, Recombinant Genes Genetic Engineering Genetic engineering Genome Genomes Molekulargenetik DNS Gentechnologie |
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