Molecular forensics:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Chichester [u.a.]
Wiley
2007
|
| Schlagworte: | |
| Online-Zugang: | Table of contents only Publisher description Contributor biographical information Inhaltsverzeichnis |
| Beschreibung: | XIII, 244 S. Ill., graph. Darst. |
| ISBN: | 9780470024959 9780470024966 047002495X 0470024968 |
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| 245 | 1 | 0 | |a Molecular forensics |c ed. by Ralph Rapley ... |
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| 300 | |a XIII, 244 S. |b Ill., graph. Darst. | ||
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Datensatz im Suchindex
| _version_ | 1804137184601571328 |
|---|---|
| adam_text | Contents
Preface ix
List of Contributors xi
1 Current and Future Trends in Forensic Molecular Biology 1
Simon J. Walsh
1.1 Introduction 1
1.2 Developments within the field of forensic molecular biology 2
1.3 Developments influencing law enforcement operational impacts 7
1.4 Developments influencing the justice system socio legal impacts 10
1.5 Summary 11
1.6 References 12
2 Basic Tools and Techniques in Molecular Biology 21
Ralph Rapley and David Whitehouse
2.1 Introduction 21
2.2 Isolation and separation of nucleic acids 21
2.3 Automated analysis of nucleic acid fragments 23
2.4 Molecular biology and bioinformatics 24
2.5 The polymerase chain reaction (PCR) 25
2.6 Applications of the PCR 28
2.7 Nucleotide sequencing of DNA 30
2.8 Conclusion 34
2.9 References 34
3 Automated DNA Extraction Techniques for Forensic Analysis 37
Marion Nagy
3.1 Introduction 37
3.2 Principal steps of DNA extraction 38
3.3 DNA extraction techniques 39
3.4 Modified techniques for DNA extraction from challenging forensic
samples 43
3.5 Automation of DNA extraction 45
3.6 References 56
4 Real time Quantitative PCR in Forensic Science 59
Antonio Alonso and Oscar Garcia
4.1 Introduction 59
4.2 Current real time PCR chemistries 60
vl CONTENTS
4.3 Human nuclear DNA quantification 61
4.4 Human mitochondrial DNA quantification 65
4.5 Detection and quantification of non human species 66
4.6 Concluding remarks and perspectives 67
4.7 References 67
5 Minisatellite and Microsatellite DNA Typing Analysis 71
Keiji Tamaki
5.1 Introduction 71
5.2 Minisatellites 71
5.3 Microsatellites 80
5.4 Acknowledgements 86
5.5 References 86
6 Application of SNPs in Forensic Casework 91
Claus Bersting, Juan J. Sanchez and Niels Morling
6.1 Introduction 91
6.2 Single nucleotide polymorphisms 92
6.3 Single nucleotide polymorphism typing technology 94
6.4 Single nucleotide polymorphisms for human identification 95
6.5 Single nucleotide polymorphisms in mitochondrial DNA 98
6.6 Forensic DNA phenotyping 98
6.7 Ethical considerations of SNP genotyping 100
6.8 References 100
7 The X Chromosome in Forensic Science: Past, Present
and Future 103
Reinhard Szibor
7.1 Introduction 103
7.2 History of forensic utilization of the X chromosome 104
7.3 Chromosome X short tandem repeats 107
7.4 Power of ChrX markers in trace analysis 111
7.5 Power of ChrX markers in kinship testing 111
7.6 Chromosome X marker mapping and haplotype analysis 114
7.7 Chromosome X chromosome Y homologue markers 119
7.8 Chromosome X STR allele and haplotype distribution in different
populations 119
7.9 Ethical considerations in ChrX marker testing 120
7.10 Concluding remarks 121
7.11 References 121
8 Mitochondrial Analysis in Forensic Science 127
Hirokazu Matsuda and Nobuhiro Yukawa
8.1 Introduction 127
8.2 Mitochondrial DNA (mtDNA) biology 128
8.3 Identification of individuals (mtDNA typing) 132
8.4 Topics of forensic interest 134
8.5 References 138
CONTENTS vii
9 Y Chromosomal Markers in Forensic Genetics 141
Manfred Kayser
9.1 Introduction 141
9.2 Identification of the male sex 142
9.3 Identification of male lineages 143
9.4 Identification of a male s paternity 148
9.5 Identification of a male s geographical origin 151
9.6 The future of Y chromosomal markers in forensics 155
9.7 Acknowledgements 156
9.8 References 157
10 Laser Microdissection in Forensic Science 163
Luigi Saravo, Davide Di Martino, Nicola Staiti, Carlo Romano,
Enrico Di Luise, Dario Piscitello, Salvatore Spitaleri,
Ernesto Ginestra, Ignazio Ciuna, Fabio Quadrana, Beniamino Leo,
Giuseppe Giuffre and Giovanni Tuccari
10.1 Introduction 163
10.2 Histological, biochemical analysis 166
10.3 References 169
11 Laboratory Information Systems for Forensic Analysis of
DNA Evidence 171
Benoit Leclair and Tom Scholl
11.1 Introduction 171
11.2 The specifications of forensic genotyping assays 173
11.3 Automated pipetting 174
11.4 Analysis of STR data 176
11.5 Bioinformatics 178
11.6 Conclusion 180
11.7 References 181
12 Statistical Presentation of Forensic Data 185
Mark A. Best
12. Introduction 185
12.2 Techniques 185
12.3 Laboratory issues 188
12.4 Statistical analysis 189
12.5 Other issues 193
12.6 Special situations 194
12.7 References 195
13 Protein Profiling for Forensic and Biometric Applications 197
Mikhail Soloviev, Julian Bailes, Nina Salata and Paul Finch
13.1 Introduction 197
13.2 Protein assays in molecular forensics: current status 197
13.3 Novel technologies and the remaining challenges 204
13.4 Protein markers for use in forensic and biometric applications 208
13.5 References 2i7
vili CONTENTS
14 Application of MRS in Forensic Pathology 221
Eva Scheurer, Michael Ith and Chris Boesch
14.1 Forensic, criminalistic and ethical significance of time of death 221
14.2 Classical methods for the determination of PMI 222
14.3 Magnetic resonance spectroscopy 224
14.4 How to predict PMI based on MRS measurements 229
14.5 Outlook 232
14.6 References 235
Index 241
|
| adam_txt |
Contents
Preface ix
List of Contributors xi
1 Current and Future Trends in Forensic Molecular Biology 1
Simon J. Walsh
1.1 Introduction 1
1.2 Developments within the field of forensic molecular biology 2
1.3 Developments influencing law enforcement operational impacts 7
1.4 Developments influencing the justice system socio legal impacts 10
1.5 Summary 11
1.6 References 12
2 Basic Tools and Techniques in Molecular Biology 21
Ralph Rapley and David Whitehouse
2.1 Introduction 21
2.2 Isolation and separation of nucleic acids 21
2.3 Automated analysis of nucleic acid fragments 23
2.4 Molecular biology and bioinformatics 24
2.5 The polymerase chain reaction (PCR) 25
2.6 Applications of the PCR 28
2.7 Nucleotide sequencing of DNA 30
2.8 Conclusion 34
2.9 References 34
3 Automated DNA Extraction Techniques for Forensic Analysis 37
Marion Nagy
3.1 Introduction 37
3.2 Principal steps of DNA extraction 38
3.3 DNA extraction techniques 39
3.4 Modified techniques for DNA extraction from challenging forensic
samples 43
3.5 Automation of DNA extraction 45
3.6 References 56
4 Real time Quantitative PCR in Forensic Science 59
Antonio Alonso and Oscar Garcia
4.1 Introduction 59
4.2 Current real time PCR chemistries 60
vl CONTENTS
4.3 Human nuclear DNA quantification 61
4.4 Human mitochondrial DNA quantification 65
4.5 Detection and quantification of non human species 66
4.6 Concluding remarks and perspectives 67
4.7 References 67
5 Minisatellite and Microsatellite DNA Typing Analysis 71
Keiji Tamaki
5.1 Introduction 71
5.2 Minisatellites 71
5.3 Microsatellites 80
5.4 Acknowledgements 86
5.5 References 86
6 Application of SNPs in Forensic Casework 91
Claus Bersting, Juan J. Sanchez and Niels Morling
6.1 Introduction 91
6.2 Single nucleotide polymorphisms 92
6.3 Single nucleotide polymorphism typing technology 94
6.4 Single nucleotide polymorphisms for human identification 95
6.5 Single nucleotide polymorphisms in mitochondrial DNA 98
6.6 Forensic DNA phenotyping 98
6.7 Ethical considerations of SNP genotyping 100
6.8 References 100
7 The X Chromosome in Forensic Science: Past, Present
and Future 103
Reinhard Szibor
7.1 Introduction 103
7.2 History of forensic utilization of the X chromosome 104
7.3 Chromosome X short tandem repeats 107
7.4 Power of ChrX markers in trace analysis 111
7.5 Power of ChrX markers in kinship testing 111
7.6 Chromosome X marker mapping and haplotype analysis 114
7.7 Chromosome X chromosome Y homologue markers 119
7.8 Chromosome X STR allele and haplotype distribution in different
populations 119
7.9 Ethical considerations in ChrX marker testing 120
7.10 Concluding remarks 121
7.11 References 121
8 Mitochondrial Analysis in Forensic Science 127
Hirokazu Matsuda and Nobuhiro Yukawa
8.1 Introduction 127
8.2 Mitochondrial DNA (mtDNA) biology 128
8.3 Identification of individuals (mtDNA typing) 132
8.4 Topics of forensic interest 134
8.5 References 138
CONTENTS vii
9 Y Chromosomal Markers in Forensic Genetics 141
Manfred Kayser
9.1 Introduction 141
9.2 Identification of the male sex 142
9.3 Identification of male lineages 143
9.4 Identification of a male's paternity 148
9.5 Identification of a male's geographical origin 151
9.6 The future of Y chromosomal markers in forensics 155
9.7 Acknowledgements 156
9.8 References 157
10 Laser Microdissection in Forensic Science 163
Luigi Saravo, Davide Di Martino, Nicola Staiti, Carlo Romano,
Enrico Di Luise, Dario Piscitello, Salvatore Spitaleri,
Ernesto Ginestra, Ignazio Ciuna, Fabio Quadrana, Beniamino Leo,
Giuseppe Giuffre and Giovanni Tuccari
10.1 Introduction 163
10.2 Histological, biochemical analysis 166
10.3 References 169
11 Laboratory Information Systems for Forensic Analysis of
DNA Evidence 171
Benoit Leclair and Tom Scholl
11.1 Introduction 171
11.2 The specifications of forensic genotyping assays 173
11.3 Automated pipetting 174
11.4 Analysis of STR data 176
11.5 Bioinformatics 178
11.6 Conclusion 180
11.7 References 181
12 Statistical Presentation of Forensic Data 185
Mark A. Best
12.\ Introduction 185
12.2 Techniques 185
12.3 Laboratory issues 188
12.4 Statistical analysis 189
12.5 Other issues 193
12.6 Special situations 194
12.7 References 195
13 Protein Profiling for Forensic and Biometric Applications 197
Mikhail Soloviev, Julian Bailes, Nina Salata and Paul Finch
13.1 Introduction 197
13.2 Protein assays in molecular forensics: current status 197
13.3 Novel technologies and the remaining challenges 204
13.4 Protein markers for use in forensic and biometric applications 208
13.5 References 2i7
vili CONTENTS
14 Application of MRS in Forensic Pathology 221
Eva Scheurer, Michael Ith and Chris Boesch
14.1 Forensic, criminalistic and ethical significance of time of death 221
14.2 Classical methods for the determination of PMI 222
14.3 Magnetic resonance spectroscopy 224
14.4 How to predict PMI based on MRS measurements 229
14.5 Outlook 232
14.6 References 235
Index 241 |
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| spelling | Molecular forensics ed. by Ralph Rapley ... Chichester [u.a.] Wiley 2007 XIII, 244 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Forensic biology Chemistry, Forensic Forensic Medicine methods DNA analysis Genetic Techniques Molekularbiologie (DE-588)4039983-7 gnd rswk-swf Rechtsmedizin (DE-588)4048805-6 gnd rswk-swf Rechtsmedizin (DE-588)4048805-6 s Molekularbiologie (DE-588)4039983-7 s b DE-604 Rapley, Ralph Sonstige oth http://www.loc.gov/catdir/toc/ecip075/2006038357.html Table of contents only http://www.loc.gov/catdir/enhancements/fy0740/2006038357-d.html Publisher description http://www.loc.gov/catdir/enhancements/fy0740/2006038357-b.html Contributor biographical information HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016149495&sequence=000010&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Molecular forensics Forensic biology Chemistry, Forensic Forensic Medicine methods DNA analysis Genetic Techniques Molekularbiologie (DE-588)4039983-7 gnd Rechtsmedizin (DE-588)4048805-6 gnd |
| subject_GND | (DE-588)4039983-7 (DE-588)4048805-6 |
| title | Molecular forensics |
| title_auth | Molecular forensics |
| title_exact_search | Molecular forensics |
| title_exact_search_txtP | Molecular forensics |
| title_full | Molecular forensics ed. by Ralph Rapley ... |
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| title_full_unstemmed | Molecular forensics ed. by Ralph Rapley ... |
| title_short | Molecular forensics |
| title_sort | molecular forensics |
| topic | Forensic biology Chemistry, Forensic Forensic Medicine methods DNA analysis Genetic Techniques Molekularbiologie (DE-588)4039983-7 gnd Rechtsmedizin (DE-588)4048805-6 gnd |
| topic_facet | Forensic biology Chemistry, Forensic Forensic Medicine methods DNA analysis Genetic Techniques Molekularbiologie Rechtsmedizin |
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