Protocols for nucleic acid analysis by nonradioactive probes:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2007
|
| Ausgabe: | 2. ed. |
| Schriftenreihe: | Methods in molecular biology
353 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | X, 321 S. Ill., graph. Darst. |
| ISBN: | 9781588294302 1588294307 |
Internformat
MARC
| LEADER | 00000nam a2200000zcb4500 | ||
|---|---|---|---|
| 001 | BV022934787 | ||
| 003 | DE-604 | ||
| 005 | 20080901 | ||
| 007 | t | ||
| 008 | 071023s2007 xxuad|| |||| 00||| eng d | ||
| 010 | |a 2006003449 | ||
| 020 | |a 9781588294302 |9 978-1-58829-430-2 | ||
| 020 | |a 1588294307 |c alk. paper |9 1-58829-430-7 | ||
| 035 | |a (OCoLC)63680478 | ||
| 035 | |a (DE-599)DNB 2006003449 | ||
| 040 | |a DE-604 |b ger |e aacr | ||
| 041 | 0 | |a eng | |
| 044 | |a xxu |c US | ||
| 049 | |a DE-703 |a DE-M49 | ||
| 050 | 0 | |a QP620 | |
| 082 | 0 | |a 572.8/636 |2 22 | |
| 084 | |a WC 4150 |0 (DE-625)148092: |2 rvk | ||
| 084 | |a CHE 808f |2 stub | ||
| 084 | |a CHE 830f |2 stub | ||
| 245 | 1 | 0 | |a Protocols for nucleic acid analysis by nonradioactive probes |c ed. by Elena Hilario ... |
| 250 | |a 2. ed. | ||
| 264 | 1 | |a Totowa, NJ |b Humana Press |c 2007 | |
| 300 | |a X, 321 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in molecular biology |v 353 | |
| 650 | 7 | |a Chemische analyses |2 gtt | |
| 650 | 7 | |a Nucleïnezuren |2 gtt | |
| 650 | 7 | |a Wetenschappelijke technieken |2 gtt | |
| 650 | 4 | |a Wissenschaftliches Arbeiten | |
| 650 | 4 | |a DNA Probes |v Laboratory Manuals | |
| 650 | 4 | |a DNA |x analysis |v Laboratory Manuals | |
| 650 | 4 | |a Molecular Probe Techniques |v Laboratory Manuals | |
| 650 | 4 | |a Nucleic Acid Amplification Techniques |v Laboratory Manuals | |
| 650 | 4 | |a Nucleic acid probes | |
| 650 | 4 | |a Nucleic acids |x Analysis | |
| 650 | 0 | 7 | |a Chemische Analyse |0 (DE-588)4009840-0 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Nucleinsäuren |0 (DE-588)4172117-2 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4143413-4 |a Aufsatzsammlung |2 gnd-content | |
| 689 | 0 | 0 | |a Nucleinsäuren |0 (DE-588)4172117-2 |D s |
| 689 | 0 | 1 | |a Chemische Analyse |0 (DE-588)4009840-0 |D s |
| 689 | 0 | |C b |5 DE-604 | |
| 700 | 1 | |a Hilario, Elena |d 1966- |e Sonstige |0 (DE-588)132998327 |4 oth | |
| 830 | 0 | |a Methods in molecular biology |v 353 |w (DE-604)BV035362695 |9 353 | |
| 856 | 4 | 2 | |m HBZ Datenaustausch |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016139556&sequence=000012&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-016139556 | ||
Datensatz im Suchindex
| _version_ | 1804137168340254720 |
|---|---|
| adam_text | Contents
Preface v
Contributors ix
Part I. Nucleic Acid Extractions
1 Genomic DNA Isolation From Different Biological Materials
Duckchul Park 3
2 Extraction of Plant RNA
Elspeth MacRae 15
Part II. Indirect Detection
3 Overview of Hybridization and Detection Techniques
Elena Hilario 27
4 Checkerboard DNA-DNA Hybridization Technology
Using Digoxigenin Detection
Lisa S. Gellen, Glenn M. Wall-Manning, and Chris H. Sissons 39
5 Nonradioactive Northern and Southern Analyses
From Plant Samples
Christoph Peterhaensel, Dagmar Weier, and Thomas Lahaye 69
6 Screening a BAC Library With Nonradioactive Overlapping
Oligonucleotide (Overgo) Probes
Elena Hilario, Tiffany F. Bennell, and Erik Rikkerink 79
7 Direct In-Gel Hybridization of DNA With
Digoxigenin-Labeled Probes
Saeed A. Khan and Mohamed S. Nawaz 93
8 In Situ Hybridization of Termite Microbes
Shigeharu Moriya, Satoko Noda, Moriya Ohkuma,
and Toshiaki Kudo 705
Part III. Fluorescent Labeling and Detection
9 RNA Electrophoretic Mobility Shift Assay Using a Fluorescent
DNA Sequencer
Yukinori Eguchi 115
vii
viii Contents
10 Comparative Quantitation of mRNA Expression
in the Central Nervous System Using Fluorescence
In Situ Hybridization
Darren J. Day, Eli M. Mrkusich, and John H. Miller 125
11 Visualization of Gene Expression by Fluorescent Multiplex
Reverse Transcriptase-PCR Amplification
Maria Rosa Ponce, Victor Quesada, Andrea Hricova,
and Jose Luis Micol 143
12 Fluorescence In Situ Hybridization for the Identification
of Environmental Microbes
Annelie Pernthaler and Jakob Pernthaler 153
Part IV. Kinetic ( Real-Time ) PCR
13 Introduction to Kinetic (Real-Time) PCR
John Mackay 167
14 Validation of Short Interfering RNA Knockdowns by Quantitative
Real-Time PCR
Sukru Tuzmen, Jeff Kiefer, and Spyro Mousses 177
15 Real-Time Quantitative PCR as an Alternative to Southern Blot
or Fluorescence In Situ Hybridization for Detection
of Gene Copy Number Changes
Jasmien Hoebeeck, Frank Speleman, and Jo Vandesompele 205
16 Design and Work-Up of a New Molecular Diagnostic Assay
Based on Real-Time PCR
Harald H. Kessler 227
1 7 Real-Time PCR Fluorescent Chemistries
John Mackay and Olfert Landt 237
Part V. Microarrays
18 Microarrays: An Overview
Norman H. Lee and Alexander I. Saeed 265
19 Oligonucleotide Microarrays for the Study of Coastal
Microbial Communities
Gaspar Taroncher-Oldenburg and Bess B. Ward 301
Index 317
|
| adam_txt |
Contents
Preface v
Contributors ix
Part I. Nucleic Acid Extractions
1 Genomic DNA Isolation From Different Biological Materials
Duckchul Park 3
2 Extraction of Plant RNA
Elspeth MacRae 15
Part II. Indirect Detection
3 Overview of Hybridization and Detection Techniques
Elena Hilario 27
4 Checkerboard DNA-DNA Hybridization Technology
Using Digoxigenin Detection
Lisa S. Gellen, Glenn M. Wall-Manning, and Chris H. Sissons 39
5 Nonradioactive Northern and Southern Analyses
From Plant Samples
Christoph Peterhaensel, Dagmar Weier, and Thomas Lahaye 69
6 Screening a BAC Library With Nonradioactive Overlapping
Oligonucleotide (Overgo) Probes
Elena Hilario, Tiffany F. Bennell, and Erik Rikkerink 79
7 Direct In-Gel Hybridization of DNA With
Digoxigenin-Labeled Probes
Saeed A. Khan and Mohamed S. Nawaz 93
8 In Situ Hybridization of Termite Microbes
Shigeharu Moriya, Satoko Noda, Moriya Ohkuma,
and Toshiaki Kudo 705
Part III. Fluorescent Labeling and Detection
9 RNA Electrophoretic Mobility Shift Assay Using a Fluorescent
DNA Sequencer
Yukinori Eguchi 115
vii
viii Contents
10 Comparative Quantitation of mRNA Expression
in the Central Nervous System Using Fluorescence
In Situ Hybridization
Darren J. Day, Eli M. Mrkusich, and John H. Miller 125
11 Visualization of Gene Expression by Fluorescent Multiplex
Reverse Transcriptase-PCR Amplification
Maria Rosa Ponce, Victor Quesada, Andrea Hricova,
and Jose Luis Micol 143
12 Fluorescence In Situ Hybridization for the Identification
of Environmental Microbes
Annelie Pernthaler and Jakob Pernthaler 153
Part IV. Kinetic ("Real-Time") PCR
13 Introduction to Kinetic (Real-Time) PCR
John Mackay 167
14 Validation of Short Interfering RNA Knockdowns by Quantitative
Real-Time PCR
Sukru Tuzmen, Jeff Kiefer, and Spyro Mousses 177
15 Real-Time Quantitative PCR as an Alternative to Southern Blot
or Fluorescence In Situ Hybridization for Detection
of Gene Copy Number Changes
Jasmien Hoebeeck, Frank Speleman, and Jo Vandesompele 205
16 Design and Work-Up of a New Molecular Diagnostic Assay
Based on Real-Time PCR
Harald H. Kessler 227
1 7 Real-Time PCR Fluorescent Chemistries
John Mackay and Olfert Landt 237
Part V. Microarrays
18 Microarrays: An Overview
Norman H. Lee and Alexander I. Saeed 265
19 Oligonucleotide Microarrays for the Study of Coastal
Microbial Communities
Gaspar Taroncher-Oldenburg and Bess B. Ward 301
Index 317 |
| any_adam_object | 1 |
| any_adam_object_boolean | 1 |
| author_GND | (DE-588)132998327 |
| building | Verbundindex |
| bvnumber | BV022934787 |
| callnumber-first | Q - Science |
| callnumber-label | QP620 |
| callnumber-raw | QP620 |
| callnumber-search | QP620 |
| callnumber-sort | QP 3620 |
| callnumber-subject | QP - Physiology |
| classification_rvk | WC 4150 |
| classification_tum | CHE 808f CHE 830f |
| ctrlnum | (OCoLC)63680478 (DE-599)DNB 2006003449 |
| dewey-full | 572.8/636 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 572 - Biochemistry |
| dewey-raw | 572.8/636 |
| dewey-search | 572.8/636 |
| dewey-sort | 3572.8 3636 |
| dewey-tens | 570 - Biology |
| discipline | Biologie Chemie |
| discipline_str_mv | Biologie Chemie |
| edition | 2. ed. |
| format | Book |
| fullrecord | <?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>02254nam a2200589zcb4500</leader><controlfield tag="001">BV022934787</controlfield><controlfield tag="003">DE-604</controlfield><controlfield tag="005">20080901 </controlfield><controlfield tag="007">t</controlfield><controlfield tag="008">071023s2007 xxuad|| |||| 00||| eng d</controlfield><datafield tag="010" ind1=" " ind2=" "><subfield code="a">2006003449</subfield></datafield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">9781588294302</subfield><subfield code="9">978-1-58829-430-2</subfield></datafield><datafield tag="020" ind1=" " ind2=" "><subfield code="a">1588294307</subfield><subfield code="c">alk. paper</subfield><subfield code="9">1-58829-430-7</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(OCoLC)63680478</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DNB 2006003449</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-604</subfield><subfield code="b">ger</subfield><subfield code="e">aacr</subfield></datafield><datafield tag="041" ind1="0" ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="044" ind1=" " ind2=" "><subfield code="a">xxu</subfield><subfield code="c">US</subfield></datafield><datafield tag="049" ind1=" " ind2=" "><subfield code="a">DE-703</subfield><subfield code="a">DE-M49</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">QP620</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">572.8/636</subfield><subfield code="2">22</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">WC 4150</subfield><subfield code="0">(DE-625)148092:</subfield><subfield code="2">rvk</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">CHE 808f</subfield><subfield code="2">stub</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">CHE 830f</subfield><subfield code="2">stub</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Protocols for nucleic acid analysis by nonradioactive probes</subfield><subfield code="c">ed. by Elena Hilario ...</subfield></datafield><datafield tag="250" ind1=" " ind2=" "><subfield code="a">2. ed.</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="a">Totowa, NJ</subfield><subfield code="b">Humana Press</subfield><subfield code="c">2007</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">X, 321 S.</subfield><subfield code="b">Ill., graph. Darst.</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="490" ind1="1" ind2=" "><subfield code="a">Methods in molecular biology</subfield><subfield code="v">353</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Chemische analyses</subfield><subfield code="2">gtt</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Nucleïnezuren</subfield><subfield code="2">gtt</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Wetenschappelijke technieken</subfield><subfield code="2">gtt</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Wissenschaftliches Arbeiten</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">DNA Probes</subfield><subfield code="v">Laboratory Manuals</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">DNA</subfield><subfield code="x">analysis</subfield><subfield code="v">Laboratory Manuals</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Molecular Probe Techniques</subfield><subfield code="v">Laboratory Manuals</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Nucleic Acid Amplification Techniques</subfield><subfield code="v">Laboratory Manuals</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Nucleic acid probes</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Nucleic acids</subfield><subfield code="x">Analysis</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Chemische Analyse</subfield><subfield code="0">(DE-588)4009840-0</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="650" ind1="0" ind2="7"><subfield code="a">Nucleinsäuren</subfield><subfield code="0">(DE-588)4172117-2</subfield><subfield code="2">gnd</subfield><subfield code="9">rswk-swf</subfield></datafield><datafield tag="655" ind1=" " ind2="7"><subfield code="0">(DE-588)4143413-4</subfield><subfield code="a">Aufsatzsammlung</subfield><subfield code="2">gnd-content</subfield></datafield><datafield tag="689" ind1="0" ind2="0"><subfield code="a">Nucleinsäuren</subfield><subfield code="0">(DE-588)4172117-2</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2="1"><subfield code="a">Chemische Analyse</subfield><subfield code="0">(DE-588)4009840-0</subfield><subfield code="D">s</subfield></datafield><datafield tag="689" ind1="0" ind2=" "><subfield code="C">b</subfield><subfield code="5">DE-604</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Hilario, Elena</subfield><subfield code="d">1966-</subfield><subfield code="e">Sonstige</subfield><subfield code="0">(DE-588)132998327</subfield><subfield code="4">oth</subfield></datafield><datafield tag="830" ind1=" " ind2="0"><subfield code="a">Methods in molecular biology</subfield><subfield code="v">353</subfield><subfield code="w">(DE-604)BV035362695</subfield><subfield code="9">353</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="m">HBZ Datenaustausch</subfield><subfield code="q">application/pdf</subfield><subfield code="u">http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016139556&sequence=000012&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA</subfield><subfield code="3">Inhaltsverzeichnis</subfield></datafield><datafield tag="999" ind1=" " ind2=" "><subfield code="a">oai:aleph.bib-bvb.de:BVB01-016139556</subfield></datafield></record></collection> |
| genre | (DE-588)4143413-4 Aufsatzsammlung gnd-content |
| genre_facet | Aufsatzsammlung |
| id | DE-604.BV022934787 |
| illustrated | Illustrated |
| index_date | 2024-07-02T18:55:58Z |
| indexdate | 2024-07-09T21:08:00Z |
| institution | BVB |
| isbn | 9781588294302 1588294307 |
| language | English |
| lccn | 2006003449 |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-016139556 |
| oclc_num | 63680478 |
| open_access_boolean | |
| owner | DE-703 DE-M49 DE-BY-TUM |
| owner_facet | DE-703 DE-M49 DE-BY-TUM |
| physical | X, 321 S. Ill., graph. Darst. |
| publishDate | 2007 |
| publishDateSearch | 2007 |
| publishDateSort | 2007 |
| publisher | Humana Press |
| record_format | marc |
| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spelling | Protocols for nucleic acid analysis by nonradioactive probes ed. by Elena Hilario ... 2. ed. Totowa, NJ Humana Press 2007 X, 321 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 353 Chemische analyses gtt Nucleïnezuren gtt Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten DNA Probes Laboratory Manuals DNA analysis Laboratory Manuals Molecular Probe Techniques Laboratory Manuals Nucleic Acid Amplification Techniques Laboratory Manuals Nucleic acid probes Nucleic acids Analysis Chemische Analyse (DE-588)4009840-0 gnd rswk-swf Nucleinsäuren (DE-588)4172117-2 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Nucleinsäuren (DE-588)4172117-2 s Chemische Analyse (DE-588)4009840-0 s b DE-604 Hilario, Elena 1966- Sonstige (DE-588)132998327 oth Methods in molecular biology 353 (DE-604)BV035362695 353 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016139556&sequence=000012&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Protocols for nucleic acid analysis by nonradioactive probes Methods in molecular biology Chemische analyses gtt Nucleïnezuren gtt Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten DNA Probes Laboratory Manuals DNA analysis Laboratory Manuals Molecular Probe Techniques Laboratory Manuals Nucleic Acid Amplification Techniques Laboratory Manuals Nucleic acid probes Nucleic acids Analysis Chemische Analyse (DE-588)4009840-0 gnd Nucleinsäuren (DE-588)4172117-2 gnd |
| subject_GND | (DE-588)4009840-0 (DE-588)4172117-2 (DE-588)4143413-4 |
| title | Protocols for nucleic acid analysis by nonradioactive probes |
| title_auth | Protocols for nucleic acid analysis by nonradioactive probes |
| title_exact_search | Protocols for nucleic acid analysis by nonradioactive probes |
| title_exact_search_txtP | Protocols for nucleic acid analysis by nonradioactive probes |
| title_full | Protocols for nucleic acid analysis by nonradioactive probes ed. by Elena Hilario ... |
| title_fullStr | Protocols for nucleic acid analysis by nonradioactive probes ed. by Elena Hilario ... |
| title_full_unstemmed | Protocols for nucleic acid analysis by nonradioactive probes ed. by Elena Hilario ... |
| title_short | Protocols for nucleic acid analysis by nonradioactive probes |
| title_sort | protocols for nucleic acid analysis by nonradioactive probes |
| topic | Chemische analyses gtt Nucleïnezuren gtt Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten DNA Probes Laboratory Manuals DNA analysis Laboratory Manuals Molecular Probe Techniques Laboratory Manuals Nucleic Acid Amplification Techniques Laboratory Manuals Nucleic acid probes Nucleic acids Analysis Chemische Analyse (DE-588)4009840-0 gnd Nucleinsäuren (DE-588)4172117-2 gnd |
| topic_facet | Chemische analyses Nucleïnezuren Wetenschappelijke technieken Wissenschaftliches Arbeiten DNA Probes Laboratory Manuals DNA analysis Laboratory Manuals Molecular Probe Techniques Laboratory Manuals Nucleic Acid Amplification Techniques Laboratory Manuals Nucleic acid probes Nucleic acids Analysis Chemische Analyse Nucleinsäuren Aufsatzsammlung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016139556&sequence=000012&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV035362695 |
| work_keys_str_mv | AT hilarioelena protocolsfornucleicacidanalysisbynonradioactiveprobes |