RNA modification:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Amsterdam [u.a.]
Elsevier, Acad. Press
2007
|
| Schriftenreihe: | Methods in enzymology
425 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XLII, 433 S., [4] Bl. Ill., graph. Darst. |
| ISBN: | 9780123741554 0123741556 |
Internformat
MARC
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| 001 | BV022881920 | ||
| 003 | DE-604 | ||
| 005 | 20071106 | ||
| 007 | t | ||
| 008 | 071015s2007 ad|| |||| 00||| eng d | ||
| 020 | |a 9780123741554 |9 978-0-12-374155-4 | ||
| 020 | |a 0123741556 |9 0-12-374155-6 | ||
| 035 | |a (OCoLC)170033061 | ||
| 035 | |a (DE-599)BVBBV022881920 | ||
| 040 | |a DE-604 |b ger |e rakwb | ||
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| 049 | |a DE-19 |a DE-20 |a DE-355 |a DE-91G |a DE-12 |a DE-703 | ||
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| 084 | |a WC 4355 |0 (DE-625)148096: |2 rvk | ||
| 084 | |a CHE 860f |2 stub | ||
| 245 | 1 | 0 | |a RNA modification |c ed. by Jonatha M. Gott |
| 264 | 1 | |a Amsterdam [u.a.] |b Elsevier, Acad. Press |c 2007 | |
| 300 | |a XLII, 433 S., [4] Bl. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 1 | |a Methods in enzymology |v 425 | |
| 650 | 4 | |a RNA editing | |
| 650 | 0 | 7 | |a Nucleotide |0 (DE-588)4135085-6 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a RNS |0 (DE-588)4076759-0 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Modifikation |g Biochemie |0 (DE-588)4307844-8 |2 gnd |9 rswk-swf |
| 689 | 0 | 0 | |a RNS |0 (DE-588)4076759-0 |D s |
| 689 | 0 | 1 | |a Nucleotide |0 (DE-588)4135085-6 |D s |
| 689 | 0 | 2 | |a Modifikation |g Biochemie |0 (DE-588)4307844-8 |D s |
| 689 | 0 | |C b |5 DE-604 | |
| 700 | 1 | |a Gott, Jonatha M. |e Sonstige |4 oth | |
| 830 | 0 | |a Methods in enzymology |v 425 |w (DE-604)BV000000938 |9 425 | |
| 856 | 4 | 2 | |m Digitalisierung UB Regensburg |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016086882&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
| 999 | |a oai:aleph.bib-bvb.de:BVB01-016086882 | ||
Datensatz im Suchindex
| _version_ | 1804137145982517248 |
|---|---|
| adam_text | Contents
Contributors
x¡
Preface
xv
Volumes in Series
xvii
Section I. Modified Nucleotides
ι
1. Identifying Modifications in
RNA
by MALDI Mass Spectrometry
3
Stephen Douthwaite and Finn Kirpekar
а. Introduction
4
2.
Experimental Strategy
5
3.
Experimental Procedures
7
4.
Perspectives and Conclusion
15
References
17
2.
Identification of Modified Residues in RNAs by Reverse
Transcription-Based Methods
21
Yuri Motorin,
Sébastien
Muller,
Isabelle
Behm-Ansmant, and
Christiane
Branlant
1.
Introduction
22
2.
Reverse Transcription (RT)-Based
Methods for Detection of
Modified Residues
24
3.
RNA
Extraction from Various Cell Types
29
4.
RNA
Modifications Detectable After Specific Chemical Treatment
32
5.
RNA
Modifications Leading to RT Pauses Without Preliminary
Chemical Treatment
48
б.
Conclusion
48
References
49
v¡
Contents
Section II. tRNA Modifications
55
3.
Detection of Enzymatic Activity of Transfer
RNA
Modification
Enzymes Using
Radiolabeled tRNA
Substrates
57
Henri Grosjean, Louis Droogmans,
Martine
Roovers, and
Gérard
Keith
1.
Introduction
59
2.
Identification of Modified Nucleotides in tRNA
60
3.
Testing the Activity of
RNA
Modification Enzymes
62
4.
Complete Digestion of
RNA
with Various Nucleases
70
5.
Analysis of
RNA
Digest Products by Thin-Layer Chromatography
(TLC)
73
6.
Detection of Modified Nucleotides in Uniformly Labeled [32P]-RNA or
[35S]-Containing
RNA
Extracted from Microorganisms
82
7.
Base Composition Analysis of
RNA
by Postlabeling Procedures
84
8.
RNA
Sequencing of Purified Nonradiolabeled tRNA Species
87
9.
Identification of Modified Residues in RNAs by Reverse
Transcriptase-Based Methods
90
10.
Discussion and Outlook
92
References
94
4.
In Vitro Detection of the Enzymatic Activity of
Folate-Dependent tRNA (Uracil-54,-C5)-Methyltransferase:
Evolutionary Implications
103
Jaunius Urbonavicius,
Céline Brochier-Armanet, Stéphane Skouloubris,
Hannu Myllykallio, and Henri Grosjean
1.
Introduction
104
2.
Overproduction and Purification of B. subtilis
tRNA (Uracil-54,-C5)-Methyltransferase
106
3.
Enzymatic Activity Assay
107
4.
Phylogenetic Analysis no
5.
Discussion 11a
References
117
5.
Probing the Intermediacy of Covalent
RNA
Enzyme Complexes in
RNA
Modification Enzymes
121
Stephanie M. Chervin, Jeffrey D.
Kittendorf,
and George A. Garcia
1.
Introduction
122
2.
A Case Study for Probing Reaction Intermediacy: The Covalent
RNA
Complex of tRNA-Guanine Transglycosylase (TGT)
124
3.
Denaturing Gel Electrophoresis: A Tool to Probe
Enzyme-RNA Complexes
125
Contents
vii
4.
Analysis of the Chemical Competency of Covalent
Enzyme-RNA Complexes
132
5.
Analysis of the Rate of Formation of Covalent
Enzyme-RNA Complexes
133
6.
Summary
135
References
136
6.
Identification and Characterization of Modification Enzymes by
Biochemical Analysis of the Proteome
139
Jane E. Jackman, Lakmal Kotelawala, Elizabeth
).
Grayhack,
and Eric M. Phizicky
1.
Introduction
140
2.
Methodology
141
3.
Development of an Appropriate Biochemical Assay
141
4.
Parallel Biochemical Analysis of the Yeast Proteome with the
MORF
Library
144
5.
Deconvolution of the Active Pool to Identify the Individual
Gene Responsible for Activity
147
6.
Confirmation of the Identity of the Gene Responsible for the
Modification Activity
147
7.
Possible Reasons for Lack of Success with this Approach
149
8.
Conclusions
150
References
150
7.
Identification of Genes Encoding tRNA Modification Enzymes by
Comparative Genomics
153
Valérie de Crécy-Lagard
1.
Introduction
154
2.
Methods to Identify Missing tRNA Modification Genes
155
3.
Homology-Based Genomic Data Mining Methods
159
4.
Non-Homology-Based Genomic Data Mining Methods
160
5.
General Conclusion: The Power of Integration
176
References
176
8.
Identification and Characterization of Archaeal and Fungal
tRNA Methyltransferases
185
David E. Graham and
Gisela
Kramer
a. Introduction
186
2.
Identification of tRNA Methyltransferases by Use of
Genome Sequences
187
viii Contents
3. Substrates
for Methyltransferase Assays
192
4. Methyltransferase
Activity Assays
198
5.
Identifying Modification Products
200
6.
Identifying Modification Sites
201
7.
tRNA Methyltransferases from M. jannaschi!
205
References
205
9.
Mass Spectrometric Identification and Characterization of
RNA-Modifying Enzymes
211
Tsutomu Suzuki, Yoshiho Ikeuchi, Akiko
Noma,
Takeo Suzuki, and
Yuriko Sakaguchi
1.
Introduction
212
2.
Ribonucleome Analysis: Quest for RNA-Modifying Genes
212
3.
Strains Used for the Ribonucleome Analysis
214
4.
Parallel Preparation of Total RNAs from
£
coli or
Yeast Strains
214
5.
Isolation and Purification of Individual tRNAs from Yeast
216
6.
Nucleoside Preparation
217
7.
Mass Spectrometric Analysis of Total Nucleosides
217
8.
LC/MS Profiling of Modified Nucleosides
219
9.
RNase Digestion for
RNA
Fragment Analysis by LC/MS
221
10.
Capillary LC
NANO
ESI/Mass Spectrometry
221
11.
RNA
Fragment Analysis by Capillary LC/MS
223
12.
Discussion
224
References
227
10.
Chaplet Column Chromatography: Isolation of a Large Set of
Individual RNAs in a Single Step
231
Takeo Suzuki and Tsutomu Suzuki
1.
Introduction
231
2.
Outline of Chaplet Column Chromatography
232
3.
Materials and Reagents
234
4.
Preparation of Crude
RNA
Solution
235
5.
Immobilization of
DNA
Probes
235
6.
RNA
Isolation by the
CCC
Method
237
7.
Discussion
238
References
238
Contents
¡χ
Section
III. Sno-Mediated Modifications
241
11.
Biochemical Purification of Box H/ACA RNPs Involved in
Pseudouridylation
243
John Karijolich, David Stephenson, and Yi-Tao Yu
1.
Introduction
244
2.
Biotin-Streptavidin Affinity Purification Using Biotinylated
SFU-Containing
RNA
246
3.
TAP Tag Purification of Box H/ACA RNPs
251
4.
Immunoprecipitation of Box H/ACA RNPs
258
References
260
12.
In Vitro
Reconstitution
and Affinity Purification of Catalytically
Active Archaeal Box C/D sRNP Complexes
263
Keith Gagnon, Xinxin Zhang, and E. Stuart Maxwell
1.
Introduction
264
2.
Cloning, Expression, and Preparation of M. jannaschii Box C/D
sRNP Core Proteins
265
3.
Cloning and In Vitro Transcription of Archaeal Box C/D sRNAs
270
4.
In Vitro Assembly of the M, jannaschii sR8 Box C/D sRNP Complex
270
5.
Assessment of In Vitro Assembled M.
jannaschii
Box C/D sRNP
Methylation Activity
271
6.
Sequential Affinity
Chromatographie
Purification of In Vitro
Assembled Box C/D sRNPs
272
7.
Concluding Remarks
280
References
281
13.
Identifying Effects of snoRNA-Guided Modifications on the
Synthesis and Function of the Yeast Ribosome
283
Wayne A. Decatur, Xue-hai Liang,
Dorota Piekna-Przybylska,
and
Maurille J. Fournier
1.
Introduction
284
2.
Experimental Strategies
285
3.
Analyzing snoRNA-Guided Modifications in Selected
Regions of the Ribosome
287
4.
Determining Effects on rRNA and Ribosome Biogenesis
297
5.
Investigating Ribosomal Complexes
302
6.
Characterizing Ribosome Function
304
7.
Chemical Probing of rRNA Structure
306
References
308
Contents
14.
The Ui
snRNA
Hairpin II as
a RNA
Affinity Tag for Selecting
snoRNP Complexes
317
Dorota
Piekna-Przybylska, Ben Liu, and Maurille J. Fournier
1.
Introduction
318
2.
Methodology
326
3.
Materials and Experimental Procedures
342
References
348
15.
A Dedicated Computational Approach for the Identification of
Archaeal H/ACA sRNAs
355
Sébastien
Muller,
Bruno
Charpentier,
Christiane
Branlant, and Fabrice
Ledere
1.
Introduction
356
2.
Method
358
3.
Conclusions
383
References
384
16.
Reconstitution
of
Archaeal H/ACA
sRNPs and Test of their Activity
389
Bruno
Charpentier, jean-Baptiste
Fourmann, and
Christiane
Branlant
1.
Introduction
390
2.
Overview of the Issues that can be Addressed by the Method
393
3.
The Basic Principles of the Experiments
393
4,
Materials and Reagents
394
5.
Methods
396
6.
General Comments
403
References
404
Author Index
407
Subject Index
427
|
| adam_txt |
Contents
Contributors
x¡
Preface
xv
Volumes in Series
xvii
Section I. Modified Nucleotides
ι
1. Identifying Modifications in
RNA
by MALDI Mass Spectrometry
3
Stephen Douthwaite and Finn Kirpekar
а. Introduction
4
2.
Experimental Strategy
5
3.
Experimental Procedures
7
4.
Perspectives and Conclusion
15
References
17
2.
Identification of Modified Residues in RNAs by Reverse
Transcription-Based Methods
21
Yuri Motorin,
Sébastien
Muller,
Isabelle
Behm-Ansmant, and
Christiane
Branlant
1.
Introduction
22
2.
Reverse Transcription (RT)-Based
Methods for Detection of
Modified Residues
24
3.
RNA
Extraction from Various Cell Types
29
4.
RNA
Modifications Detectable After Specific Chemical Treatment
32
5.
RNA
Modifications Leading to RT Pauses Without Preliminary
Chemical Treatment
48
б.
Conclusion
48
References
49
v¡
Contents
Section II. tRNA Modifications
55
3.
Detection of Enzymatic Activity of Transfer
RNA
Modification
Enzymes Using
Radiolabeled tRNA
Substrates
57
Henri Grosjean, Louis Droogmans,
Martine
Roovers, and
Gérard
Keith
1.
Introduction
59
2.
Identification of Modified Nucleotides in tRNA
60
3.
Testing the Activity of
RNA
Modification Enzymes
62
4.
Complete Digestion of
RNA
with Various Nucleases
70
5.
Analysis of
RNA
Digest Products by Thin-Layer Chromatography
(TLC)
73
6.
Detection of Modified Nucleotides in Uniformly Labeled [32P]-RNA or
[35S]-Containing
RNA
Extracted from Microorganisms
82
7.
Base Composition Analysis of
RNA
by Postlabeling Procedures
84
8.
RNA
Sequencing of Purified Nonradiolabeled tRNA Species
87
9.
Identification of Modified Residues in RNAs by Reverse
Transcriptase-Based Methods
90
10.
Discussion and Outlook
92
References
94
4.
In Vitro Detection of the Enzymatic Activity of
Folate-Dependent tRNA (Uracil-54,-C5)-Methyltransferase:
Evolutionary Implications
103
Jaunius Urbonavicius,
Céline Brochier-Armanet, Stéphane Skouloubris,
Hannu Myllykallio, and Henri Grosjean
1.
Introduction
104
2.
Overproduction and Purification of B. subtilis
tRNA (Uracil-54,-C5)-Methyltransferase
106
3.
Enzymatic Activity Assay
107
4.
Phylogenetic Analysis no
5.
Discussion 11a
References
117
5.
Probing the Intermediacy of Covalent
RNA
Enzyme Complexes in
RNA
Modification Enzymes
121
Stephanie M. Chervin, Jeffrey D.
Kittendorf,
and George A. Garcia
1.
Introduction
122
2.
A Case Study for Probing Reaction Intermediacy: The Covalent
RNA
Complex of tRNA-Guanine Transglycosylase (TGT)
124
3.
Denaturing Gel Electrophoresis: A Tool to Probe
Enzyme-RNA Complexes
125
Contents
vii
4.
Analysis of the Chemical Competency of Covalent
Enzyme-RNA Complexes
132
5.
Analysis of the Rate of Formation of Covalent
Enzyme-RNA Complexes
133
6.
Summary
135
References
136
6.
Identification and Characterization of Modification Enzymes by
Biochemical Analysis of the Proteome
139
Jane E. Jackman, Lakmal Kotelawala, Elizabeth
).
Grayhack,
and Eric M. Phizicky
1.
Introduction
140
2.
Methodology
141
3.
Development of an Appropriate Biochemical Assay
141
4.
Parallel Biochemical Analysis of the Yeast Proteome with the
MORF
Library
144
5.
Deconvolution of the Active Pool to Identify the Individual
Gene Responsible for Activity
147
6.
Confirmation of the Identity of the Gene Responsible for the
Modification Activity
147
7.
Possible Reasons for Lack of Success with this Approach
149
8.
Conclusions
150
References
150
7.
Identification of Genes Encoding tRNA Modification Enzymes by
Comparative Genomics
153
Valérie de Crécy-Lagard
1.
Introduction
154
2.
Methods to Identify Missing tRNA Modification Genes
155
3.
Homology-Based Genomic Data Mining Methods
159
4.
Non-Homology-Based Genomic Data Mining Methods
160
5.
General Conclusion: The Power of Integration
176
References
176
8.
Identification and Characterization of Archaeal and Fungal
tRNA Methyltransferases
185
David E. Graham and
Gisela
Kramer
a. Introduction
186
2.
Identification of tRNA Methyltransferases by Use of
Genome Sequences
187
viii Contents
3. Substrates
for Methyltransferase Assays
192
4. Methyltransferase
Activity Assays
198
5.
Identifying Modification Products
200
6.
Identifying Modification Sites
201
7.
tRNA Methyltransferases from M. jannaschi!
205
References
205
9.
Mass Spectrometric Identification and Characterization of
RNA-Modifying Enzymes
211
Tsutomu Suzuki, Yoshiho Ikeuchi, Akiko
Noma,
Takeo Suzuki, and
Yuriko Sakaguchi
1.
Introduction
212
2.
Ribonucleome Analysis: Quest for RNA-Modifying Genes
212
3.
Strains Used for the Ribonucleome Analysis
214
4.
Parallel Preparation of Total RNAs from
£
coli or
Yeast Strains
214
5.
Isolation and Purification of Individual tRNAs from Yeast
216
6.
Nucleoside Preparation
217
7.
Mass Spectrometric Analysis of Total Nucleosides
217
8.
LC/MS Profiling of Modified Nucleosides
219
9.
RNase Digestion for
RNA
Fragment Analysis by LC/MS
221
10.
Capillary LC
NANO
ESI/Mass Spectrometry
221
11.
RNA
Fragment Analysis by Capillary LC/MS
223
12.
Discussion
224
References
227
10.
Chaplet Column Chromatography: Isolation of a Large Set of
Individual RNAs in a Single Step
231
Takeo Suzuki and Tsutomu Suzuki
1.
Introduction
231
2.
Outline of Chaplet Column Chromatography
232
3.
Materials and Reagents
234
4.
Preparation of Crude
RNA
Solution
235
5.
Immobilization of
DNA
Probes
235
6.
RNA
Isolation by the
CCC
Method
237
7.
Discussion
238
References
238
Contents
¡χ
Section
III. Sno-Mediated Modifications
241
11.
Biochemical Purification of Box H/ACA RNPs Involved in
Pseudouridylation
243
John Karijolich, David Stephenson, and Yi-Tao Yu
1.
Introduction
244
2.
Biotin-Streptavidin Affinity Purification Using Biotinylated
SFU-Containing
RNA
246
3.
TAP Tag Purification of Box H/ACA RNPs
251
4.
Immunoprecipitation of Box H/ACA RNPs
258
References
260
12.
In Vitro
Reconstitution
and Affinity Purification of Catalytically
Active Archaeal Box C/D sRNP Complexes
263
Keith Gagnon, Xinxin Zhang, and E. Stuart Maxwell
1.
Introduction
264
2.
Cloning, Expression, and Preparation of M. jannaschii Box C/D
sRNP Core Proteins
265
3.
Cloning and In Vitro Transcription of Archaeal Box C/D sRNAs
270
4.
In Vitro Assembly of the M, jannaschii sR8 Box C/D sRNP Complex
270
5.
Assessment of In Vitro Assembled M.
jannaschii
Box C/D sRNP
Methylation Activity
271
6.
Sequential Affinity
Chromatographie
Purification of In Vitro
Assembled Box C/D sRNPs
272
7.
Concluding Remarks
280
References
281
13.
Identifying Effects of snoRNA-Guided Modifications on the
Synthesis and Function of the Yeast Ribosome
283
Wayne A. Decatur, Xue-hai Liang,
Dorota Piekna-Przybylska,
and
Maurille J. Fournier
1.
Introduction
284
2.
Experimental Strategies
285
3.
Analyzing snoRNA-Guided Modifications in Selected
Regions of the Ribosome
287
4.
Determining Effects on rRNA and Ribosome Biogenesis
297
5.
Investigating Ribosomal Complexes
302
6.
Characterizing Ribosome Function
304
7.
Chemical Probing of rRNA Structure
306
References
308
Contents
14.
The Ui
snRNA
Hairpin II as
a RNA
Affinity Tag for Selecting
snoRNP Complexes
317
Dorota
Piekna-Przybylska, Ben Liu, and Maurille J. Fournier
1.
Introduction
318
2.
Methodology
326
3.
Materials and Experimental Procedures
342
References
348
15.
A Dedicated Computational Approach for the Identification of
Archaeal H/ACA sRNAs
355
Sébastien
Muller,
Bruno
Charpentier,
Christiane
Branlant, and Fabrice
Ledere
1.
Introduction
356
2.
Method
358
3.
Conclusions
383
References
384
16.
Reconstitution
of
Archaeal H/ACA
sRNPs and Test of their Activity
389
Bruno
Charpentier, jean-Baptiste
Fourmann, and
Christiane
Branlant
1.
Introduction
390
2.
Overview of the Issues that can be Addressed by the Method
393
3.
The Basic Principles of the Experiments
393
4,
Materials and Reagents
394
5.
Methods
396
6.
General Comments
403
References
404
Author Index
407
Subject Index
427 |
| any_adam_object | 1 |
| any_adam_object_boolean | 1 |
| building | Verbundindex |
| bvnumber | BV022881920 |
| classification_rvk | VK 8700 WC 4355 |
| classification_tum | CHE 860f |
| ctrlnum | (OCoLC)170033061 (DE-599)BVBBV022881920 |
| dewey-full | 572.8845 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 572 - Biochemistry |
| dewey-raw | 572.8845 |
| dewey-search | 572.8845 |
| dewey-sort | 3572.8845 |
| dewey-tens | 570 - Biology |
| discipline | Chemie / Pharmazie Biologie Chemie |
| discipline_str_mv | Chemie / Pharmazie Biologie Chemie |
| format | Book |
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| id | DE-604.BV022881920 |
| illustrated | Illustrated |
| index_date | 2024-07-02T18:50:47Z |
| indexdate | 2024-07-09T21:07:39Z |
| institution | BVB |
| isbn | 9780123741554 0123741556 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-016086882 |
| oclc_num | 170033061 |
| open_access_boolean | |
| owner | DE-19 DE-BY-UBM DE-20 DE-355 DE-BY-UBR DE-91G DE-BY-TUM DE-12 DE-703 |
| owner_facet | DE-19 DE-BY-UBM DE-20 DE-355 DE-BY-UBR DE-91G DE-BY-TUM DE-12 DE-703 |
| physical | XLII, 433 S., [4] Bl. Ill., graph. Darst. |
| publishDate | 2007 |
| publishDateSearch | 2007 |
| publishDateSort | 2007 |
| publisher | Elsevier, Acad. Press |
| record_format | marc |
| series | Methods in enzymology |
| series2 | Methods in enzymology |
| spelling | RNA modification ed. by Jonatha M. Gott Amsterdam [u.a.] Elsevier, Acad. Press 2007 XLII, 433 S., [4] Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in enzymology 425 RNA editing Nucleotide (DE-588)4135085-6 gnd rswk-swf RNS (DE-588)4076759-0 gnd rswk-swf Modifikation Biochemie (DE-588)4307844-8 gnd rswk-swf RNS (DE-588)4076759-0 s Nucleotide (DE-588)4135085-6 s Modifikation Biochemie (DE-588)4307844-8 s b DE-604 Gott, Jonatha M. Sonstige oth Methods in enzymology 425 (DE-604)BV000000938 425 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016086882&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | RNA modification Methods in enzymology RNA editing Nucleotide (DE-588)4135085-6 gnd RNS (DE-588)4076759-0 gnd Modifikation Biochemie (DE-588)4307844-8 gnd |
| subject_GND | (DE-588)4135085-6 (DE-588)4076759-0 (DE-588)4307844-8 |
| title | RNA modification |
| title_auth | RNA modification |
| title_exact_search | RNA modification |
| title_exact_search_txtP | RNA modification |
| title_full | RNA modification ed. by Jonatha M. Gott |
| title_fullStr | RNA modification ed. by Jonatha M. Gott |
| title_full_unstemmed | RNA modification ed. by Jonatha M. Gott |
| title_short | RNA modification |
| title_sort | rna modification |
| topic | RNA editing Nucleotide (DE-588)4135085-6 gnd RNS (DE-588)4076759-0 gnd Modifikation Biochemie (DE-588)4307844-8 gnd |
| topic_facet | RNA editing Nucleotide RNS Modifikation Biochemie |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016086882&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV000000938 |
| work_keys_str_mv | AT gottjonatham rnamodification |