Verfügbarkeit: RNA editing :: THWS Bibkatalog

RNA editing:

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Bibliographische Detailangaben
Format:	Buch
Sprache:	English
Veröffentlicht:	Amsterdam [u.a.] Elsevier, Acad. Press 2007
Schriftenreihe:	Methods in enzymology 424
Schlagworte:	RNA editing Labortechnik RNS-Edierung Aufsatzsammlung
Online-Zugang:	Inhaltsverzeichnis
Beschreibung:	XLV, 544 S., [5] Bl. Ill., graph. Darst.
ISBN:	9780123739223 0123739225

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Datensatz im Suchindex

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adam_text	Contents Contributors xiii Preface xix Volumes in Series xxi Section I. Trypanosome U Insertion/Deletion ι 1. Isolation and Compositional Analysis of Trypanosomatid Editosomes 3 Aswini K. Panigrahi, Achim Schnaufer, and Kenneth D. Stuart 1. Introduction 4 2. Isolation of Enzymatically Active Editosomes 4 3. Identification of Protein Components 10 4. Tandem Affinity Purification of Editosomes 15 5. Characterization of Complexes 18 References 22 2. Uridine Insertion/Deletion Editing Activities 25 Jason Carnes and Kenneth D. Stuart 1. Introduction 26 2. Full-Round Insertion and Deletion 27 3. Precleaved Insertion and Deletion 35 4. TUTase Activity 40 5. Ligase Activity 41 6. Helicase Activity 42 7. Real-Time PCR of Editing In Vivo 43 References 52 3. RNA Editing Uridylyltransferases of Trypanosomatids 55 Ruslan Aphasizhev and Inna Aphasizheva 1. Introduction 56 2. Purification of the RNA Editing TUTase 1 from Mitochondria of Leishmania tarentolae 58 3. Expression and Purification of Recombinant RETi 62 4. Isolation of the Editosome-Embedded and Recombinant RNA Editing TUTase 2 64 5. Affinity Isolation of RET2-Contaiпίпg Complex from T. brucei 65 vi Contents 6. Expression and Purification of the Recombinant TbRET2 68 7. Uridylyltransferase Activity Assays for the 20S Editosome and Recombinant RET2 69 8. In Vitro Analysis of Protein-Protein Interactions Involving TbRET2 71 References 72 4. Isolation of RNA Binding Proteins Involved in Insertion/Deletion Editing 75 Michel Pelletier, Laurie K. Read, and Ruslan Aphasizhev 1. Introduction 76 2. Purification and Characterization of Mitochondrial RNA Binding Proteins 1 and 2 from Leishmania tarentolae 80 3. Purification of the MRP1/2 Complex from Leishmania tarentolae 81 4. Analysis of MRP1/2 Interactions in Mitochondrial Extracts 86 5. Reconstitution of the MRP1/2 Complex from Recombinant Proteins 89 6. RNA Binding and RNA Annealing Activities of the MRP1/2 Complex 91 7. Purification of RBP16 from Procyclic Form Trypansoma brucei 94 8. Purification of RBP16 from the Bloodstream Forms Trypansoma brucei 97 9. Expression and Purification of Recombinant RBP16 99 References 103 5. RNA-Protein Interactions in Assembled Editing Complexes in Trypanosomes 107 Jorge Cruz-Reyes 1. Introduction 108 2. Site-Specific Photocrosslinking Methodology to Detect Direct Interactions of Purified Editing Complexes with Substrates for Full-Round Editing 110 3. Assays to Assess the Specificity of RNA-Protein Crosslinking Interactions in Editing Complexes 117 4. Additional Applications 121 5. Concluding Remarks 123 References 123 6. Strategies of Kinetoplastid Cryptogene Discovery and Analysis 127 Dmitri A. Maslov and Larry Simpson 1. Introduction 128 2. Cell Growth 129 3. DNA Isolation 131 4. Search for Cryptogenes 132 5. Elucidation of Edited mRNA Sequences 134 6. Search for Guide RNA Genes 135 References 136 Contents vii Section II. Physarum Insertion Editing 141 7. RNA Editing in Physarum Mitochondria: Assays and Biochemical Approaches 143 Elaine M. Byrne, Linda Visomirski-Robic, Yu-Wei Cheng, Amy C. Rhee, and Jonatha M. Gott 1. Introduction 144 2. Experimental Systems 145 3. RNA Editing Assays 158 References 171 8. Computational Approaches to Insertional RNA Editing 173 Ralf Bundschuh 1. Introduction 174 2. Overview 175 3. Building Gene Models 176 4. Locating Genes 178 5. Determining Start and Stop Codons 183 6. Predicting Editing Site Locations 185 7. Conclusions 193 References 194 9. Evolution of RNA Editing Sites in the Mitochondrial Small Subunit rRNA of the Myxomycota 197 Uma Krishnan, Arpi Barsamian, and Dennis L. Miller 1. Introduction 198 2. Isolation and Amplification of Myxomycete DNA for Sequence Analysis 201 3. Analysis of Mitochondrial DNA and cDNA Sequences of the SSL) rRNA Core Region 204 References 218 Section III. 5 Deletion/Insertion Editing of tRNAs 221 10. Analysis of 5 - or 3 -Terminal tRNA Editing: Mitochondrial 5 tRNA Editing in Acanthamoeba castellami as the Exemplar 223 Amanda J. Lohan and Michael W. Gray 1. Introduction 224 2. Methods 226 3. Concluding Remarks 240 References 240 v¡¡¡ Contents Section IV. A-to-l Editing 243 11. Comparative Genomic and Bioinformatic Approaches for the Identification of New Adenosine-to-lnosine Substrates 245 Jaimie Sixsmith and Robert A. Reenan 1. Adenosine-to-lnosine RNA Editing 246 2. Bioinformatics 248 3. Comparative Genomics 249 4. Nervous System Targets of RNA Editing Identified by Comparative Genomics (Hoopengardner et al., 2003) 251 5. Molecular Determinants and Guided Evolution of Species-Specific RNA Editing (Reenan, 2005) 252 6. Systematic Identification of Abundant Adenosine-to-lnosine Editing Sites in the Human Transcriptome (Levanon et ai, 2004) 256 7. Evolutionary Conserved Human Targets of Adenosine-to-lnosine RNA Editing (Levanon et ai, 2005) 257 8. Is Abundant Adenosine-to-lnosine RNA Editing Primate Specific? (Eisenberg et ai, 2005) 259 9. A Bioinformatics Screen for Novel Adenosine-to-lnosine RNA Editing Sites Reveals Recoding Editing in BC10 (Clutterluck et ai, 2005) 260 10. Conclusion 261 References 262 12. Genetic Approaches to Studying Adenosine-to-lnosine RNA Editing 265 James E. C Jepson and Robert A. Reenan 1. Introduction 266 2. ADARs: Structure and Function 267 3. The Drosophila ADAR is Required for Normal Adult Behavior and Undergoes Transcriptional and Posttranscriptional Regulation 269 4. Identifying the Targets of ADAR 270 5. The Use of Genetic Techniques to Study Adenosine-to-lnosine Editing in Drosophila 273 6. Spatial and Temporal Control of Transgenes Using the UAS-GAL4, Gene-Switch, and TARGET Systems 274 7. Quantifying Adenosine-to-lnosine Editing 280 References 284 13. A Method for Finding Sites of Selective Adenosine Deamination 289 Johan Ohlson and Marie Öhman 1. Introduction 290 2. The Choice of Organism and Tissue to Find Novel Adenosine-to-lnosine Substrates in Mammals 291 Contents 3. Proteins Binding to Double-Stranded RNA 291 4. Using Immunoprecipitation to Detect ADAR-RNA Complexes 292 5. Using Microarray to Detect Adenosine-to-lnosine Editing Targets 294 6. Detection of Known Site-Selectively Edited Sites Using Specific Immunoprecipitation 294 7. Identification of Sites of Editing within the Substrate Candidates 295 8. ADAR2 Coimmunoprecipitation Using Mouse Brain 296 References 299 14. Purification and Assay of ADAR Activity 301 Liam P. Keegan, Joshua ). Rosenthal, Loretta M. Roberson, and Mary A. O Connell 1. Introduction 302 2. Overexpression of Recombinant Proteins in Pichia pastoris 302 3. Quantification of the Efficiency of RNA Editing 310 References 317 15. Large-Scale Overexpression and Purification of ADARs from Saccharomyces cerevisiae for Biophysical and Biochemical Studies 319 Mark R. Macbeth and Brenda L. Bass 1. Introduction 320 2. Methods 321 3. Analysis of Purified hADAR2 328 4. Future Perspectives 329 References 329 16. Mouse Models to Elucidate the Functional Roles of Adenosine-to-lnosine Editing 333 Elizabeth Y. Rula and Ronald B. Emeson 1. Introduction 334 2. Mammalian ADAR Enzymes 336 3. Mammalian ADAR Substrates 338 4. Generation of Genetically Modified Mice by Homologous Recombination in ES Cells 340 5. Classification of Transgenic Approaches 345 6. Genetic Modification of ADAR Substrates 345 7. Enzyme Modified Mice 353 8. Enzyme and Substrate Modified Mice 357 References 360 Contents 17. Probing Adenosine-to-lnosine Editing Reactions Using RNA-Containing Nucleoside Analogs 369 Olena Maydanovych, LaHoma M. Easterwood, Tao Cui, Eduardo A. Véliz, Subhash Pokharel, and Peter A. Beai 1. Introduction 370 2. Synthesis of a Substrate Analog Phosphoramidite 371 3. General Synthetic Procedures 371 4. Synthesis, Deprotection, and Purification of Chemically Modified ADAR Substrate RNAs 374 5. General Biochemical Procedures 375 6. Evaluation of ADAR Substrate Analogs 377 7. Synthesis of a Transition State Analog Phosphoramidite for Mechanism-Based Trapping 379 8. Mechanism-Based Trapping of ADAR-RNA Complexes 382 References 385 Section V. Nuclear C-to-U Editing 387 18. Measuring Editing Activity and Identifying Cytidine-to-Uridine mRNA Editing Factors in Celts and Biochemical Isolates 389 Harold С Smith 1. Introduction to Mammalian Cytidine-to-Uridine mRNA Editing 390 2. Poisoned Primer Extension 392 3. Biochemical Purification of Editing Factors 394 4. Activity Assays 401 5. Summary 410 References 410 19. Mouse Models as Tools to Explore Cytidine-to-Uridine RNA Editing 417 Soo-Jin Cho, Valerie Blanc, and Nicholas 0. Davidson 1. Introduction 418 2. apoB C-to-U RNA Editing 419 3. Developmental Regulation of C-to-U RNA Editing 420 4. Hormonal Regulation of C-to-U apoB RNA Editing 421 5. Nutritional Modulation of C-to-U apoB RNA Editing 424 6. Genetic Manipulations of Genes Involved in apoB RNA Editing 426 7. Analysis of apoB RNA Editing 429 References 431 Contents x¡ Section VI. Editing in Plant Organelies 437 20. RNA Editing in Plant Mitochondria: Assays and Biochemical Approaches 439 Mizuki Takenaka and Axel Brennicke 1. Introduction 440 2. Preparation of Mitochondrial Lysates Active in RNA Editing 441 3. Preparation of Templates for the RNA Editing Assays 445 4. Setting up the In Vitro Test System 448 5. The Detection System 450 6. Crucial Parameters: Troubleshooting 454 7. Adaptations and Assays for Common Applications 455 References 457 21. Assay of Editing of Exogenous RNAs in Chloroplast Extracts of Arabidopsis, Maize, Pea, and Tobacco 459 Michael L. Hayes and Maureen R. Hanson 1. Introduction 460 2. Growth Conditions for the Isolation of Intact Chloroplasts from Tobacco, Pea, Maize, and Arabidopsis 462 3. Isolation of Chloroplasts and Production of Editing Competent Extracts 464 4. Editing of Exogenous RNA In Vitro 466 5. Quantification of Editing of Exogenous RNAs 474 6. Reduction of Editing Through the Addition of Competitor RNAs 478 7. Quantification of Editing of Endogenous RNAs 480 References 481 22. In Organetto Gene Expression and RNA Editing Studies by Electroporation-Mediated Transformation of Isolated Plant Mitochondria 483 Jean-Claude Farré, David Choury, and Alejandro Araya 1. Introduction 484 2. Purification of Mitochondria 486 3. Plasmid Construction 490 4. Electroporation-Mediated Transformation of Isolated Mitochondria 492 5. Electrotransformation Assay and Posttranscriptional Modifications of the Exogenous cox II 496 References 498 xii Contents 23. Transformation of the Plastid Genome to Study RNA Editing 501 Kerry A. Lutz and Pal Maliga 1. Introduction 502 2. Identification of Unique cis Sequences for RNA Editing 503 3. Editing trans Factors 503 4. Protocols for Construction of Transplastomic Plants 504 5. Protocols for Testing RNA Editing in Tobacco Chloroplasts 513 6. Conclusions and Future Directions 515 References 516 Author Index 519 Subject Index S37
adam_txt	Contents Contributors xiii Preface xix Volumes in Series xxi Section I. Trypanosome U Insertion/Deletion ι 1. Isolation and Compositional Analysis of Trypanosomatid Editosomes 3 Aswini K. Panigrahi, Achim Schnaufer, and Kenneth D. Stuart 1. Introduction 4 2. Isolation of Enzymatically Active Editosomes 4 3. Identification of Protein Components 10 4. Tandem Affinity Purification of Editosomes 15 5. Characterization of Complexes 18 References 22 2. Uridine Insertion/Deletion Editing Activities 25 Jason Carnes and Kenneth D. Stuart 1. Introduction 26 2. Full-Round Insertion and Deletion 27 3. Precleaved Insertion and Deletion 35 4. TUTase Activity 40 5. Ligase Activity 41 6. Helicase Activity 42 7. Real-Time PCR of Editing In Vivo 43 References 52 3. RNA Editing Uridylyltransferases of Trypanosomatids 55 Ruslan Aphasizhev and Inna Aphasizheva 1. Introduction 56 2. Purification of the RNA Editing TUTase 1 from Mitochondria of Leishmania tarentolae 58 3. Expression and Purification of Recombinant RETi 62 4. Isolation of the Editosome-Embedded and Recombinant RNA Editing TUTase 2 64 5. Affinity Isolation of RET2-Contaiпίпg Complex from T. brucei 65 vi Contents 6. Expression and Purification of the Recombinant TbRET2 68 7. Uridylyltransferase Activity Assays for the 20S Editosome and Recombinant RET2 69 8. In Vitro Analysis of Protein-Protein Interactions Involving TbRET2 71 References 72 4. Isolation of RNA Binding Proteins Involved in Insertion/Deletion Editing 75 Michel Pelletier, Laurie K. Read, and Ruslan Aphasizhev 1. Introduction 76 2. Purification and Characterization of Mitochondrial RNA Binding Proteins 1 and 2 from Leishmania tarentolae 80 3. Purification of the MRP1/2 Complex from Leishmania tarentolae 81 4. Analysis of MRP1/2 Interactions in Mitochondrial Extracts 86 5. Reconstitution of the MRP1/2 Complex from Recombinant Proteins 89 6. RNA Binding and RNA Annealing Activities of the MRP1/2 Complex 91 7. Purification of RBP16 from Procyclic Form Trypansoma brucei 94 8. Purification of RBP16 from the Bloodstream Forms Trypansoma brucei 97 9. Expression and Purification of Recombinant RBP16 99 References 103 5. RNA-Protein Interactions in Assembled Editing Complexes in Trypanosomes 107 Jorge Cruz-Reyes 1. Introduction 108 2. Site-Specific Photocrosslinking Methodology to Detect Direct Interactions of Purified Editing Complexes with Substrates for Full-Round Editing 110 3. Assays to Assess the Specificity of RNA-Protein Crosslinking Interactions in Editing Complexes 117 4. Additional Applications 121 5. Concluding Remarks 123 References 123 6. Strategies of Kinetoplastid Cryptogene Discovery and Analysis 127 Dmitri A. Maslov and Larry Simpson 1. Introduction 128 2. Cell Growth 129 3. DNA Isolation 131 4. Search for Cryptogenes 132 5. Elucidation of Edited mRNA Sequences 134 6. Search for Guide RNA Genes 135 References 136 Contents vii Section II. Physarum Insertion Editing 141 7. RNA Editing in Physarum Mitochondria: Assays and Biochemical Approaches 143 Elaine M. Byrne, Linda Visomirski-Robic, Yu-Wei Cheng, Amy C. Rhee, and Jonatha M. Gott 1. Introduction 144 2. Experimental Systems 145 3. RNA Editing Assays 158 References 171 8. Computational Approaches to Insertional RNA Editing 173 Ralf Bundschuh 1. Introduction 174 2. Overview 175 3. Building Gene Models 176 4. Locating Genes 178 5. Determining Start and Stop Codons 183 6. Predicting Editing Site Locations 185 7. Conclusions 193 References 194 9. Evolution of RNA Editing Sites in the Mitochondrial Small Subunit rRNA of the Myxomycota 197 Uma Krishnan, Arpi Barsamian, and Dennis L. Miller 1. Introduction 198 2. Isolation and Amplification of Myxomycete DNA for Sequence Analysis 201 3. Analysis of Mitochondrial DNA and cDNA Sequences of the SSL) rRNA Core Region 204 References 218 Section III. 5 Deletion/Insertion Editing of tRNAs 221 10. Analysis of 5'- or 3 -Terminal tRNA Editing: Mitochondrial 5' tRNA Editing in Acanthamoeba castellami as the Exemplar 223 Amanda J. Lohan and Michael W. Gray 1. Introduction 224 2. Methods 226 3. Concluding Remarks 240 References 240 v¡¡¡ Contents Section IV. A-to-l Editing 243 11. Comparative Genomic and Bioinformatic Approaches for the Identification of New Adenosine-to-lnosine Substrates 245 Jaimie Sixsmith and Robert A. Reenan 1. Adenosine-to-lnosine RNA Editing 246 2. Bioinformatics 248 3. Comparative Genomics 249 4. Nervous System Targets of RNA Editing Identified by Comparative Genomics (Hoopengardner et al., 2003) 251 5. Molecular Determinants and Guided Evolution of Species-Specific RNA Editing (Reenan, 2005) 252 6. Systematic Identification of Abundant Adenosine-to-lnosine Editing Sites in the Human Transcriptome (Levanon et ai, 2004) 256 7. Evolutionary Conserved Human Targets of Adenosine-to-lnosine RNA Editing (Levanon et ai, 2005) 257 8. Is Abundant Adenosine-to-lnosine RNA Editing Primate Specific? (Eisenberg et ai, 2005) 259 9. A Bioinformatics Screen for Novel Adenosine-to-lnosine RNA Editing Sites Reveals Recoding Editing in BC10 (Clutterluck et ai, 2005) 260 10. Conclusion 261 References 262 12. Genetic Approaches to Studying Adenosine-to-lnosine RNA Editing 265 James E. C Jepson and Robert A. Reenan 1. Introduction 266 2. ADARs: Structure and Function 267 3. The Drosophila ADAR is Required for Normal Adult Behavior and Undergoes Transcriptional and Posttranscriptional Regulation 269 4. Identifying the Targets of ADAR 270 5. The Use of Genetic Techniques to Study Adenosine-to-lnosine Editing in Drosophila 273 6. Spatial and Temporal Control of Transgenes Using the UAS-GAL4, Gene-Switch, and TARGET Systems 274 7. Quantifying Adenosine-to-lnosine Editing 280 References 284 13. A Method for Finding Sites of Selective Adenosine Deamination 289 Johan Ohlson and Marie Öhman 1. Introduction 290 2. The Choice of Organism and Tissue to Find Novel Adenosine-to-lnosine Substrates in Mammals 291 Contents 3. Proteins Binding to Double-Stranded RNA 291 4. Using Immunoprecipitation to Detect ADAR-RNA Complexes 292 5. Using Microarray to Detect Adenosine-to-lnosine Editing Targets 294 6. Detection of Known Site-Selectively Edited Sites Using Specific Immunoprecipitation 294 7. Identification of Sites of Editing within the Substrate Candidates 295 8. ADAR2 Coimmunoprecipitation Using Mouse Brain 296 References 299 14. Purification and Assay of ADAR Activity 301 Liam P. Keegan, Joshua ). Rosenthal, Loretta M. Roberson, and Mary A. O'Connell 1. Introduction 302 2. Overexpression of Recombinant Proteins in Pichia pastoris 302 3. Quantification of the Efficiency of RNA Editing 310 References 317 15. Large-Scale Overexpression and Purification of ADARs from Saccharomyces cerevisiae for Biophysical and Biochemical Studies 319 Mark R. Macbeth and Brenda L. Bass 1. Introduction 320 2. Methods 321 3. Analysis of Purified hADAR2 328 4. Future Perspectives 329 References 329 16. Mouse Models to Elucidate the Functional Roles of Adenosine-to-lnosine Editing 333 Elizabeth Y. Rula and Ronald B. Emeson 1. Introduction 334 2. Mammalian ADAR Enzymes 336 3. Mammalian ADAR Substrates 338 4. Generation of Genetically Modified Mice by Homologous Recombination in ES Cells 340 5. Classification of Transgenic Approaches 345 6. Genetic Modification of ADAR Substrates 345 7. Enzyme Modified Mice 353 8. Enzyme and Substrate Modified Mice 357 References 360 Contents 17. Probing Adenosine-to-lnosine Editing Reactions Using RNA-Containing Nucleoside Analogs 369 Olena Maydanovych, LaHoma M. Easterwood, Tao Cui, Eduardo A. Véliz, Subhash Pokharel, and Peter A. Beai 1. Introduction 370 2. Synthesis of a Substrate Analog Phosphoramidite 371 3. General Synthetic Procedures 371 4. Synthesis, Deprotection, and Purification of Chemically Modified ADAR Substrate RNAs 374 5. General Biochemical Procedures 375 6. Evaluation of ADAR Substrate Analogs 377 7. Synthesis of a Transition State Analog Phosphoramidite for Mechanism-Based Trapping 379 8. Mechanism-Based Trapping of ADAR-RNA Complexes 382 References 385 Section V. Nuclear C-to-U Editing 387 18. Measuring Editing Activity and Identifying Cytidine-to-Uridine mRNA Editing Factors in Celts and Biochemical Isolates 389 Harold С Smith 1. Introduction to Mammalian Cytidine-to-Uridine mRNA Editing 390 2. Poisoned Primer Extension 392 3. Biochemical Purification of Editing Factors 394 4. Activity Assays 401 5. Summary 410 References 410 19. Mouse Models as Tools to Explore Cytidine-to-Uridine RNA Editing 417 Soo-Jin Cho, Valerie Blanc, and Nicholas 0. Davidson 1. Introduction 418 2. apoB C-to-U RNA Editing 419 3. Developmental Regulation of C-to-U RNA Editing 420 4. Hormonal Regulation of C-to-U apoB RNA Editing 421 5. Nutritional Modulation of C-to-U apoB RNA Editing 424 6. Genetic Manipulations of Genes Involved in apoB RNA Editing 426 7. Analysis of apoB RNA Editing 429 References 431 Contents x¡ Section VI. Editing in Plant Organelies 437 20. RNA Editing in Plant Mitochondria: Assays and Biochemical Approaches 439 Mizuki Takenaka and Axel Brennicke 1. Introduction 440 2. Preparation of Mitochondrial Lysates Active in RNA Editing 441 3. Preparation of Templates for the RNA Editing Assays 445 4. Setting up the In Vitro Test System 448 5. The Detection System 450 6. Crucial Parameters: Troubleshooting 454 7. Adaptations and Assays for Common Applications 455 References 457 21. Assay of Editing of Exogenous RNAs in Chloroplast Extracts of Arabidopsis, Maize, Pea, and Tobacco 459 Michael L. Hayes and Maureen R. Hanson 1. Introduction 460 2. Growth Conditions for the Isolation of Intact Chloroplasts from Tobacco, Pea, Maize, and Arabidopsis 462 3. Isolation of Chloroplasts and Production of Editing Competent Extracts 464 4. Editing of Exogenous RNA In Vitro 466 5. Quantification of Editing of Exogenous RNAs 474 6. Reduction of Editing Through the Addition of Competitor RNAs 478 7. Quantification of Editing of Endogenous RNAs 480 References 481 22. In Organetto Gene Expression and RNA Editing Studies by Electroporation-Mediated Transformation of Isolated Plant Mitochondria 483 Jean-Claude Farré, David Choury, and Alejandro Araya 1. Introduction 484 2. Purification of Mitochondria 486 3. Plasmid Construction 490 4. Electroporation-Mediated Transformation of Isolated Mitochondria 492 5. Electrotransformation Assay and Posttranscriptional Modifications of the Exogenous cox II 496 References 498 xii Contents 23. Transformation of the Plastid Genome to Study RNA Editing 501 Kerry A. Lutz and Pal Maliga 1. Introduction 502 2. Identification of Unique cis Sequences for RNA Editing 503 3. Editing trans Factors 503 4. Protocols for Construction of Transplastomic Plants 504 5. Protocols for Testing RNA Editing in Tobacco Chloroplasts 513 6. Conclusions and Future Directions 515 References 516 Author Index 519 Subject Index S37
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index_date	2024-07-02T18:44:58Z
indexdate	2024-07-09T21:07:15Z
institution	BVB
isbn	9780123739223 0123739225
language	English
oai_aleph_id	oai:aleph.bib-bvb.de:BVB01-016070210
oclc_num	170033082
open_access_boolean
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physical	XLV, 544 S., [5] Bl. Ill., graph. Darst.
publishDate	2007
publishDateSearch	2007
publishDateSort	2007
publisher	Elsevier, Acad. Press
record_format	marc
series	Methods in enzymology
series2	Methods in enzymology
spelling	RNA editing ed. by Jonatha M. Gott Amsterdam [u.a.] Elsevier, Acad. Press 2007 XLV, 544 S., [5] Bl. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in enzymology 424 RNA editing Labortechnik (DE-588)4123602-6 gnd rswk-swf RNS-Edierung (DE-588)4350250-7 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content RNS-Edierung (DE-588)4350250-7 s Labortechnik (DE-588)4123602-6 s b DE-604 Gott, Jonatha M. Sonstige oth Methods in enzymology 424 (DE-604)BV000000938 424 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016070210&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis
spellingShingle	RNA editing Methods in enzymology RNA editing Labortechnik (DE-588)4123602-6 gnd RNS-Edierung (DE-588)4350250-7 gnd
subject_GND	(DE-588)4123602-6 (DE-588)4350250-7 (DE-588)4143413-4
title	RNA editing
title_auth	RNA editing
title_exact_search	RNA editing
title_exact_search_txtP	RNA editing
title_full	RNA editing ed. by Jonatha M. Gott
title_fullStr	RNA editing ed. by Jonatha M. Gott
title_full_unstemmed	RNA editing ed. by Jonatha M. Gott
title_short	RNA editing
title_sort	rna editing
topic	RNA editing Labortechnik (DE-588)4123602-6 gnd RNS-Edierung (DE-588)4350250-7 gnd
topic_facet	RNA editing Labortechnik RNS-Edierung Aufsatzsammlung
url	http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=016070210&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
volume_link	(DE-604)BV000000938
work_keys_str_mv	AT gottjonatham rnaediting

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