Mitochondria:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Amsterdam [u.a.]
Elsevier, Academic Press
2007
|
| Ausgabe: | 2. ed. |
| Schriftenreihe: | Methods in cell biology
80 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XXIV, 920 S., [6] Bl. |
| ISBN: | 9780125441735 0125441738 |
Internformat
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| 245 | 1 | 0 | |a Mitochondria |c ed. by Liza A. Pon ... |
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Datensatz im Suchindex
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| adam_text | CONTENTS
Contributors
Preface
PART I Isolation and Subfractionation of Mitochondria
1.
Animal Cells and Tissue Culture Lines
Francesco Palloni
I. Introduction
II. General Features of Mitochondrial Preparations
III. Mitochondria from Beef Heart
IV. Mitochondria from Rat Liver
V. Mitochondria from Skeletal Muscle
VI. Synaptic and Nonsynaptic Mitochondria from Different Rat Brain Regions
VII.
VIII.
IX. Mitochondria from Insect Flight Muscle
X. Mitochondria from Porcine Adrenal Cortex
XI. Mitochondria from Human Platelets
XII.
XIII.
XIV.
XV. Mitochondria from Fish Erythrocytes
XVI.
XVII.
XVIII.
References
2.
Yeast Saccharomyces cerevisiac
htvan R. Boldogh and
I. Introduction
II. Isolation of Mitochondria
III. Analysis of Isolated Mitochondria
Contents
IV.
and Inner
References
3. Isolation
Α. Η.
I. Introduction
II. Growth and Preparation of Plant Material
III. Isolation of Mitochondria
IV. Density Gradient Purification of Mitochondria
V. Mitochondrial Yield, Purity, Integrity, Storage, and Function
VI. Subfractionation of Mitochondrial Compartments
VII. In
VIII.
IX. Conclusion
References
PART II Biochemical Assays of Mitochondrial Activity
4.
Denise M.
and Robert W. Taylor
I. Introduction
II. Materials and Methods
III. Interpreting Results of RC Enzymology
References
5.
Gaetano
I. Introduction
II. Measurements of Endogenous Respiration in Intact Cells
III. KCN
IV. In Situ Analysis of Mitochondrial OXPHOS
References
6.
In Situ Hybridization Staining Methods) to Visualize Mitochondria
Kurenai
I. Introduction
II. Histochemistry
Contents
III. Immunohistochemistry
IV.
References
7.
Cristofol
I. Introduction
II. ATP Synthesis Assays
III. Methodological Considerations
IV. Experimental Procedures
V. Measurement of High-Energy Phosphates in Animal Tissue
and Cultured Cells by HPLC
References
8.
Naň
I. Introduction
II. Principle
III. Procedure
IV. Results
References
9.
Michael J. Bennett
I. Introduction
II. Metabolite Measurements
III. Enzyme and Transporter Assays
IV. Metabolic Flux Studies
V. Discussion
References
10.
TCA Cycle Enzymes and PDHc
Aim Saada Reisch and
I. Introduction
H. Pyruvate Dehydrogenase Complex
HI. Citrate Synthase
IV. Aconitase
Contents
V.
VI.
VII.
VIII.
IX.
X.
XI.
References
11.
Michael
I. Introduction
II. Molecular Species of Cardiolipin
III. Cardiolipin Analysis by
IV. Cardiolipin Analysis by HPLC
V. Cardiolipin Analysis by MS
VI. Applications of Cardiolipin Measurements
References
12.
and in Reconstituted Systems
Marco
I. Introduction
II. Determining the Permeability of the Mitochondrial Outer
Membrane to Metabolites
III. VDAC Activity After
IV. Summary
References
13.
Sabine
I. Introduction
II. Determination of Cellular Fc/S Protein Formation and
III. Determination of Mitochondrial Iron Contents
IV. Reporter Assays for Analysis of Iron-Dependent Gene Expression
in S. ceret isiac
References
Contents
PART III Assays for Mitochondrial Respiratory Activity and
Permeability in Living Cells
14.
Cationic Fluorophores
John]. Lemasters and Venkat K. Ramshesh
I. Introduction
II. Quantitative Imaging of
III. Visualization of Depolarized Mitochondria
IV. Conclusion
References
15.
Paolo
and
I. Introduction
II. Targeting Strategies and Transfection
III. Calcium Reporters
IV.
V. Conclusions
References
16.
Transition of Mitochondria
I aonfal Zamzami,
I. Introduction
II. Procedures
References
17.
Christopher
I. Introduction
II. ATP Homeostasis in the Heart
III. Measurement of Mitochondrial and Cvtosolic ATP in Cardiac Myocytes
IV. Measurement of ATP in Subcellular Compartments by Luminomctery
References
Contents
PART
18.
J. S. Armstrong and M. Whiteman
I. Introduction
II. Measurement of
III. Measurement of
IV. General Problems Associated with the Measurement of
V. Conclusions
References
19.
Catalase, and Glutathione Peroxidase
Cristo/oí Vives-Bauza, Anatoly
I. Introduction
II. Experimental Procedures
References
20.
Snifi
I. Introduction
II. Apparatus
III. Calibration of the NO Electrode
IV. NO Threshold Measurement
V. Open-Flow Respirometry
VI. An In Vitro Model of Ischemia/Reperfusion Injury
VII.
References
21.
JooYcun Oh, Michelle S.Johnson, and Aimee
I. Introduction
II. Rationale
III. Methods
IV. Discussion
References
Contents xi
PART V
22.
Ali Naini
I. Introduction
II. Large-Scale Rearrangements of mtDNA
III. Detecting mtDNA Rearrangements
IV. Details of Southern Blot Hybridization Analysis
V. Depletion of mtDNA
VI. Point Mutations in mtDNA
References
23.
in Yeast and Humans
Gerald S. Shadel and Bonnie L. Scidcl-Rogol
I. Introduction
II. Diagnosis of mtDNA Replication Defects in Yeast and Human Cells
III. Analyzing Mitochondrial Transcripts In Vivo
IV. Additional Considerations
References
24.
Sión
I. Introduction
II.
III. Analytical PCR for Investigation of mtDNA in Microdissected Samples
IV. Concluding Remarks
References
25.
Sandra R. Bacman and Carlos T.
I. Introduction
II. Generation of Nuclear Donors
III. Generation of mtDNA Donors
IV. Generation of Transmitochondrial Cybrids
V. Manipulating Heteroplasmy
References
Contents
26.
Chlamydomonas reinhardtii Mitochondria
Nathalie Bonnefoy, Claire Remade, and
I. Introduction
II. Important Features of S. cerevisiae and C. reinhardtii Mitochondrial Genetics
III. Delivery of
Cells and Detection of Mitochondrial
IV. Strategies for Gene Replacement in S. cerevisiae mtDNA
V. Transformation of rho+ Cells with Plasmids or Linear
VI. Concluding Remarks
References
27.
Mice to Model Neurodegenerative Diseases
Carl A. Pinkert and Ian A. Trounce
I. Introduction
II. Spontaneous and Induced Models of Mitochondrial Disease
III. Transgenic Models
IV. Introduction of Mutant mtDNA into Mitochondria
V. The First Transmitochondrial Mice
VI. Summary/Future Directions
References
28.
P.
and J.
I. Introduction
II. Rationale
III. Methods
IV. Materials
V. Discussion
VI. Summary
References
PART VI Assays for Mitochondrial Morphology and Motility
29.
Tlwresa C. Swayne, Anna C. Gay, and Liza A
I. Introduction
II
Contents
III. Use of Immunostaining to Detect Mitochondria in
Fixed Yeast Cells
IV.
Fluorescent Fusion Proteins
V. Tagging Endogenous Proteins with Fluorescent Proteins
VI. Strategies for Visualization of Yeast Mitochondria
VII.
References
30.
Kurt
I. Introduction
II. Labeling of Mitochondria in Living Animal Cells
III. Probing Interactions Between Mitochondria and the Cytoskeleton
and Molecular Motors in Living Animal Cells
IV. Image Acquisition
V. Preparation of Cells
VI. Time-Lapse Recordings
VII.
VIII.
ÍX.
X. Appendix
References
31.
¡swan R. Boldogh,
I. Introduction
II. In Vitro
III. Sedimentation Assay for Binding of Actin to Mitochondria
References
32.
Elena Ingennaii, Shell} Mecmen, Rachel DeVay, andjodi
I. Introduction
II. Mitochondrial Fusion in Vitro
HI. Analysis of Dynamin-Related GTPase Activity In Vitro
References
Contents
PART
to Mitochondria
33.
Ilka
I. Introduction
II. Materials and Methods
III. Applications
IV. Outlook
References
34.
Johannes M. Herrmann and
I. Introduction
II. Rationale
III. Methods
References
35.
ShukryJ.
I. Introduction
II. Overview on Protein
III. Mitochondrial-Targeting Signals
IV. Bioinformatics Tools to Predict Mitochondrial Targeting Signals
V. Experimental Analysis of Mitochondrial-Targeting Signals
VI. Summary
References
36.
Diana Stcjanoi ski,
I. Introduction
II. In Vitro Synthesis of Precursor Proteins
III. The Import Reaction
IV. Steps to Resolve the Location of Imported Mitochondrial Precursors
V. Assaying Protein Complex Assembly by BN-PAGE
VI. Resolving Protein Complex Constituents
Contents
VII.
VIII.
References
PART
1.
2.
3.
Representative Organisms
4.
5.
6.
Various Stresses and Growth Conditions
Index
Volumes in Series
|
| adam_txt |
CONTENTS
Contributors
Preface
PART I Isolation and Subfractionation of Mitochondria
1.
Animal Cells and Tissue Culture Lines
Francesco Palloni
I. Introduction
II. General Features of Mitochondrial Preparations
III. Mitochondria from Beef Heart
IV. Mitochondria from Rat Liver
V. Mitochondria from Skeletal Muscle
VI. Synaptic and Nonsynaptic Mitochondria from Different Rat Brain Regions
VII.
VIII.
IX. Mitochondria from Insect Flight Muscle
X. Mitochondria from Porcine Adrenal Cortex
XI. Mitochondria from Human Platelets
XII.
XIII.
XIV.
XV. Mitochondria from Fish Erythrocytes
XVI.
XVII.
XVIII.
References
2.
Yeast Saccharomyces cerevisiac
htvan R. Boldogh and
I. Introduction
II. Isolation of Mitochondria
III. Analysis of Isolated Mitochondria
Contents
IV.
and Inner
References
3. Isolation
Α. Η.
I. Introduction
II. Growth and Preparation of Plant Material
III. Isolation of Mitochondria
IV. Density Gradient Purification of Mitochondria
V. Mitochondrial Yield, Purity, Integrity, Storage, and Function
VI. Subfractionation of Mitochondrial Compartments
VII. In
VIII.
IX. Conclusion
References
PART II Biochemical Assays of Mitochondrial Activity
4.
Denise M.
and Robert W. Taylor
I. Introduction
II. Materials and Methods
III. Interpreting Results of RC Enzymology
References
5.
Gaetano
I. Introduction
II. Measurements of Endogenous Respiration in Intact Cells
III. KCN
IV. In Situ Analysis of Mitochondrial OXPHOS
References
6.
In Situ Hybridization Staining Methods) to Visualize Mitochondria
Kurenai
I. Introduction
II. Histochemistry
Contents
III. Immunohistochemistry
IV.
References
7.
Cristofol
I. Introduction
II. ATP Synthesis Assays
III. Methodological Considerations
IV. Experimental Procedures
V. Measurement of High-Energy Phosphates in Animal Tissue
and Cultured Cells by HPLC
References
8.
Naň
I. Introduction
II. Principle
III. Procedure
IV. Results
References
9.
Michael J. Bennett
I. Introduction
II. Metabolite Measurements
III. Enzyme and Transporter Assays
IV. Metabolic Flux Studies
V. Discussion
References
10.
TCA Cycle Enzymes and PDHc
Aim Saada Reisch and
I. Introduction
H. Pyruvate Dehydrogenase Complex
HI. Citrate Synthase
IV. Aconitase
Contents
V.
VI.
VII.
VIII.
IX.
X.
XI.
References
11.
Michael
I. Introduction
II. Molecular Species of Cardiolipin
III. Cardiolipin Analysis by
IV. Cardiolipin Analysis by HPLC
V. Cardiolipin Analysis by MS
VI. Applications of Cardiolipin Measurements
References
12.
and in Reconstituted Systems
Marco
I. Introduction
II. Determining the Permeability of the Mitochondrial Outer
Membrane to Metabolites
III. VDAC Activity After
IV. Summary
References
13.
Sabine
I. Introduction
II. Determination of Cellular Fc/S Protein Formation and
III. Determination of Mitochondrial Iron Contents
IV. Reporter Assays for Analysis of Iron-Dependent Gene Expression
in S. ceret'isiac
References
Contents
PART III Assays for Mitochondrial Respiratory Activity and
Permeability in Living Cells
14.
Cationic Fluorophores
John]. Lemasters and Venkat K. Ramshesh
I. Introduction
II. Quantitative Imaging of
III. Visualization of Depolarized Mitochondria
IV. Conclusion
References
15.
Paolo
and
I. Introduction
II. Targeting Strategies and Transfection
III. Calcium Reporters
IV.
V. Conclusions
References
16.
Transition of Mitochondria
I\'aonfal Zamzami,
I. Introduction
II. Procedures
References
17.
Christopher
I. Introduction
II. ATP Homeostasis in the Heart
III. Measurement of Mitochondrial and Cvtosolic ATP in Cardiac Myocytes
IV. Measurement of ATP in Subcellular Compartments by Luminomctery
References
Contents
PART
18.
J. S. Armstrong and M. Whiteman
I. Introduction
II. Measurement of
III. Measurement of
IV. General Problems Associated with the Measurement of
V. Conclusions
References
19.
Catalase, and Glutathione Peroxidase
Cristo/oí Vives-Bauza, Anatoly
I. Introduction
II. Experimental Procedures
References
20.
Snifi
I. Introduction
II. Apparatus
III. Calibration of the NO'Electrode
IV. NO' Threshold Measurement
V. Open-Flow Respirometry
VI. An In Vitro Model of Ischemia/Reperfusion Injury
VII.
References
21.
JooYcun Oh, Michelle S.Johnson, and Aimee
I. Introduction
II. Rationale
III. Methods
IV. Discussion
References
Contents xi
PART V
22.
Ali Naini
I. Introduction
II. Large-Scale Rearrangements of mtDNA
III. Detecting mtDNA Rearrangements
IV. Details of Southern Blot Hybridization Analysis
V. Depletion of mtDNA
VI. Point Mutations in mtDNA
References
23.
in Yeast and Humans
Gerald S. Shadel and Bonnie L. Scidcl-Rogol
I. Introduction
II. Diagnosis of mtDNA Replication Defects in Yeast and Human Cells
III. Analyzing Mitochondrial Transcripts In Vivo
IV. Additional Considerations
References
24.
Sión
I. Introduction
II.
III. Analytical PCR for Investigation of mtDNA in Microdissected Samples
IV. Concluding Remarks
References
25.
Sandra R. Bacman and Carlos T.
I. Introduction
II. Generation of Nuclear Donors
III. Generation of mtDNA Donors
IV. Generation of Transmitochondrial Cybrids
V. Manipulating Heteroplasmy
References
Contents
26.
Chlamydomonas reinhardtii Mitochondria
Nathalie Bonnefoy, Claire Remade, and
I. Introduction
II. Important Features of S. cerevisiae and C. reinhardtii Mitochondrial Genetics
III. Delivery of
Cells and Detection of Mitochondrial
IV. Strategies for Gene Replacement in S. cerevisiae mtDNA
V. Transformation of rho+ Cells with Plasmids or Linear
VI. Concluding Remarks
References
27.
Mice to Model Neurodegenerative Diseases
Carl A. Pinkert and Ian A. Trounce
I. Introduction
II. Spontaneous and Induced Models of Mitochondrial Disease
III. Transgenic Models
IV. Introduction of Mutant mtDNA into Mitochondria
V. The First Transmitochondrial Mice
VI. Summary/Future Directions
References
28.
P.
and J.
I. Introduction
II. Rationale
III. Methods
IV. Materials
V. Discussion
VI. Summary
References
PART VI Assays for Mitochondrial Morphology and Motility
29.
Tlwresa C. Swayne, Anna C. Gay, and Liza A
I. Introduction
II
Contents
III. Use of Immunostaining to Detect Mitochondria in
Fixed Yeast Cells
IV.
Fluorescent Fusion Proteins
V. Tagging Endogenous Proteins with Fluorescent Proteins
VI. Strategies for Visualization of Yeast Mitochondria
VII.
References
30.
Kurt
I. Introduction
II. Labeling of Mitochondria in Living Animal Cells
III. Probing Interactions Between Mitochondria and the Cytoskeleton
and Molecular Motors in Living Animal Cells
IV. Image Acquisition
V. Preparation of Cells
VI. Time-Lapse Recordings
VII.
VIII.
ÍX.
X. Appendix
References
31.
¡swan R. Boldogh,
I. Introduction
II. In Vitro
III. Sedimentation Assay for Binding of Actin to Mitochondria
References
32.
Elena Ingennaii, Shell}' Mecmen, Rachel DeVay, andjodi
I. Introduction
II. Mitochondrial Fusion in Vitro
HI. Analysis of Dynamin-Related GTPase Activity In Vitro
References
Contents
PART
to Mitochondria
33.
Ilka
I. Introduction
II. Materials and Methods
III. Applications
IV. Outlook
References
34.
Johannes M. Herrmann and
I. Introduction
II. Rationale
III. Methods
References
35.
ShukryJ.
I. Introduction
II. Overview on Protein
III. Mitochondrial-Targeting Signals
IV. Bioinformatics Tools to Predict Mitochondrial Targeting Signals
V. Experimental Analysis of Mitochondrial-Targeting Signals
VI. Summary
References
36.
Diana Stcjanoi'ski,
I. Introduction
II. In Vitro Synthesis of Precursor Proteins
III. The Import Reaction
IV. Steps to Resolve the Location of Imported Mitochondrial Precursors
V. Assaying Protein Complex Assembly by BN-PAGE
VI. Resolving Protein Complex Constituents
Contents
VII.
VIII.
References
PART
1.
2.
3.
Representative Organisms
4.
5.
6.
Various Stresses and Growth Conditions
Index
Volumes in Series |
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| classification_rvk | WC 5100 |
| ctrlnum | (OCoLC)123081114 (DE-599)BVBBV022498566 |
| discipline | Biologie |
| discipline_str_mv | Biologie |
| edition | 2. ed. |
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| id | DE-604.BV022498566 |
| illustrated | Not Illustrated |
| index_date | 2024-07-02T17:54:35Z |
| indexdate | 2024-07-09T20:58:55Z |
| institution | BVB |
| isbn | 9780125441735 0125441738 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-015705656 |
| oclc_num | 123081114 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-20 |
| owner_facet | DE-355 DE-BY-UBR DE-20 |
| physical | XXIV, 920 S., [6] Bl. |
| publishDate | 2007 |
| publishDateSearch | 2007 |
| publishDateSort | 2007 |
| publisher | Elsevier, Academic Press |
| record_format | marc |
| series | Methods in cell biology |
| series2 | Methods in cell biology |
| spelling | Mitochondria ed. by Liza A. Pon ... 2. ed. Amsterdam [u.a.] Elsevier, Academic Press 2007 XXIV, 920 S., [6] Bl. txt rdacontent n rdamedia nc rdacarrier Methods in cell biology 80 Mitochondriën gtt Mitochondria Molekularbiologie (DE-588)4039983-7 gnd rswk-swf Labortechnik (DE-588)4123602-6 gnd rswk-swf Mitochondrium (DE-588)4124939-2 gnd rswk-swf Mitochondrium (DE-588)4124939-2 s Labortechnik (DE-588)4123602-6 s DE-604 Molekularbiologie (DE-588)4039983-7 s Pon, Liza A. Sonstige oth Methods in cell biology 80 (DE-604)BV002534878 80 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=015705656&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Mitochondria Methods in cell biology Mitochondriën gtt Mitochondria Molekularbiologie (DE-588)4039983-7 gnd Labortechnik (DE-588)4123602-6 gnd Mitochondrium (DE-588)4124939-2 gnd |
| subject_GND | (DE-588)4039983-7 (DE-588)4123602-6 (DE-588)4124939-2 |
| title | Mitochondria |
| title_auth | Mitochondria |
| title_exact_search | Mitochondria |
| title_exact_search_txtP | Mitochondria |
| title_full | Mitochondria ed. by Liza A. Pon ... |
| title_fullStr | Mitochondria ed. by Liza A. Pon ... |
| title_full_unstemmed | Mitochondria ed. by Liza A. Pon ... |
| title_short | Mitochondria |
| title_sort | mitochondria |
| topic | Mitochondriën gtt Mitochondria Molekularbiologie (DE-588)4039983-7 gnd Labortechnik (DE-588)4123602-6 gnd Mitochondrium (DE-588)4124939-2 gnd |
| topic_facet | Mitochondriën Mitochondria Molekularbiologie Labortechnik Mitochondrium |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=015705656&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV002534878 |
| work_keys_str_mv | AT ponlizaa mitochondria |