Molecular analysis of novel subunits of the anaphasepromoting complex (APC/C) in yeast:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2005
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 102 Bl. Ill., graph. Darst. 30 cm |
Internformat
MARC
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| 100 | 1 | |a Schwickart, Martin |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Molecular analysis of novel subunits of the anaphasepromoting complex (APC/C) in yeast |c von Martin Schwickart |
| 264 | 1 | |c 2005 | |
| 300 | |a 102 Bl. |b Ill., graph. Darst. |c 30 cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 502 | |a Dresden, Techn. Univ., Diss., 2005 | ||
| 650 | 4 | |a Zellzyklus - Saccharomycetaceae - Untereinheit - Ubiquitin-Protein-Ligase | |
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| 650 | 0 | 7 | |a Saccharomycetaceae |0 (DE-588)4131614-9 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Ubiquitin-Protein-Ligase |0 (DE-588)4398417-4 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Zellzyklus |0 (DE-588)4129960-7 |2 gnd |9 rswk-swf |
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| 689 | 0 | 1 | |a Zellzyklus |0 (DE-588)4129960-7 |D s |
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| 943 | 1 | |a oai:aleph.bib-bvb.de:BVB01-015608061 | |
Datensatz im Suchindex
| _version_ | 1808044579669147648 |
|---|---|
| adam_text |
TABLE
OF
CONTENTS
ZUSAMMENFASSUNG
.
6
ABSTRACT
.
7
INTRODUCTION
.
8
COORDINATION
OF
THE
CELL
CYCLE
.
9
SISTER
CHROMATID
LINKAGE
AND
SEPARATION
IN
MITOSIS
.
9
CYCLIN
DEPENDENT
KINASES
AND
THE
APC/C
REGULATE
THE
ORDER
OF
CELL
CYCLE
EVENTS.
10
REGULATION
OF
APC/C
ACTIVITY
BY
ACTIVATORS
.
11
COORDINATION
OF
APC/C
ACTIVITY
WITH
CELL
CYCLE
TRANSITIONS
.
12
THE
MITOTIC
CHECKPOINT
.
14
REGULATION
OF
MEIOSIS
.
15
THE
APC/C
IS
A
UBIQUITIN
LIGASE
.
.
17
GENERAL
MECHANISM
OF
UBIQUITIN
LIGATION
.
17
TWO
CLASSES
OF
UBIQUITIN
LIGASES
.
18
SUBUNIT
COMPOSITION
OF
THE
APC/C
.
19
THE
ENZYMATIC
CORE
.
19
SUBUNITS
IMPLICATED
IN
PROTEIN-PROTEIN
INTERACTION
.
20
SUBSTRATE
RECOGNITION
OF
THE
APC/C
.
23
GOAL
OF
THIS
THESIS
.
24
RESULTS
.
25
IDENTIFICATION
OF
NOVEL
APC/C-ASSOCIATED
PROTEINS.
SWML
AND
MND2
.
25
CHARACTERISATION
OF
SWML
.
26
SWML
IS
A
SUBUNIT
OF
THE
APC/C
.
26
BLAST
SEARCH
FOR
HOMOLOGUES
OF
SWML/APCL3
.
28
THE
SCHIZOSACCHAROMYCES
POMBE
ORTHOLOGUE
OF
SWML/APCL3
IS
A
SUBUNIT
OF
THE
APC/C
.
29
THE
FUNCTION
OF
S.
CEREVISIAE
SWML/APCL3
IS
CONSERVED
IN
HUMANS
AND
S.
POMBE.
31
SWML/APCL3
IS
REQUIRED
FOR
STRUCTURAL
INTEGRITY
OF
THE
APC/C
.
32
SWML/APCL3
IS
REQUIRED
FOR
THE
STABLE
FORMATION
OF
A
SUB-COMPLEX
OF
THE
APC/C.
35
COMPONENTS
FOR
RECONSTITUTION
OF
APC/C
UBIQUITIN
LIGASE
ACTIVITY
IN
VITRO
.
36
SWML/APCL3
IS
REQUIRED
FOR POLYUBIQUITINATION
.
39
DEFECTIVE
CELL
CYCLE
PROGRESSION
IN
SWMIA
MUTANT
CELLS
.
41
SWML/APCL3
IS
REQUIRED
FOR
TIMELY
SISTER
CHROMATID
SEPARATION.
41
SWML/APCL3
IS
REQUIRED
FOR
APC/C
ACTIVITY
DURING
G1
.
44
CELL
SEPARATION
DEFECT
OF
THE
SWMLA
MUTANT
.
45
SWML/APCL3
IS
IMPORTANT
FOR
APC/C
ACTIVITY
IN
MEIOSIS
.
46
CHARACTERISATION
OF
MND2
.
48
MND2
IS
A
CORE
SUBUNIT
OF
THE
APC/C
IN
PROLIFERATING
CELLS
.
48
PREPARATIVE
PURIFICATION
OF
APC/C
IN
MEIOSIS
.
50
SUBUNIT
COMPOSITION
OF
THE
APC/C
IN
MEIOTIC
PROPHASE
1
.
53
MND2
IS
A
CORE
SUBUNIT
IN
MEIOTIC
PROPHASE
1.
54
MND2
IS
REQUIRED
FOR
INHIBITION
OF
PDSL
UBIQUITINATION
BY
APC/C-AMAL
IN
VITRO.
56
MND2
IS
SUFFICIENT
FOR
INHIBITION
OF
APC/C-AMAL
DEPENDENT
UBIQUITINATION
.
58
MND2
AND
APC/C-AMAL
ACTIVITY
IS
REGULATED
IN
MEIOSIS
.
61
DISCUSSION
.
64
IDENTIFICATION
OF
THE
NOVEL
APC/C
SUBUNITS
SWML
AND
MND2
.
64
EVOLUTIONARY
CONSERVATION
OF
SWML
.
64
SWML/APCL3
STABILIZES
A
SUB-COMPLEX
OF
THE
APC/C
.
65
FUNCTIONS
OF
SWM1/APC13
IN
THE
MITOTIC
CELL
CYCLE
.
67
FUNCTIONS
OF
SWM1/APC13
IN
MEIOSIS
.
67
MOLECULAR
FUNCTION
OF
MND2
.
68
MECHANISMS
OF
MND2
INHIBITION
.
69
REGULATION
OF
APC/C-AMAL
ACTIVITY
DURING
MEIOSIS
.
71
IMPLICATIONS
ON
COHESION
MAINTENANCE
.
72
MATERIAL
AND
METHODS
.
73
STRAINS
AND
GROWTH
CONDITIONS
.
73
PLASMID
CONSTRUCTION
.
73
CONSTRUCTION
OF
S.
CEREVISIAE
STRAINS
.74
4
CONSTRUCTION
OF
S.
POMBE
STRAINS
.
75
ANALYSIS
OF
PROTEINS
.
75
ANTIBODIES
.
75
APC/C
PURIFICATION
AND
MASS
SPECTROMETRY
.
76
PURIFICATION
OF
UBC4
AND
MND2
.
77
UBIQUITINATION
ASSAY
.
77
DNA
CONTENT
.
78
INDIRECT
IMMUNOFLUORESCENCE
.
78
MONITORING
OF
SISTER
CHROMATID
SEPARATION
.
78
ISOLATION
OF
CELLS
IN
G1
BY
ELUTRIATION
.
78
NUCLEAR
LOCALISATION
OF
GFP
FUSION
PROTEINS
.
79
DATABASE
SEARCHES
.
79
LARGE
SCALE
SYNCHRONOUS
MEIOTIC
CULTURES
OF
S.
CEREVISIAE
CELLS
.
79
TABLE
2.
YEAST
STRAIN
TABLE
.
80
ABBREVIATIONS
.
85
REFERENCES
.
87
ACKNOWLEDGMENTS
.
101
DECLARATION
.
102
5 |
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| discipline_str_mv | Biologie |
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| physical | 102 Bl. Ill., graph. Darst. 30 cm |
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| spelling | Schwickart, Martin Verfasser aut Molecular analysis of novel subunits of the anaphasepromoting complex (APC/C) in yeast von Martin Schwickart 2005 102 Bl. Ill., graph. Darst. 30 cm txt rdacontent n rdamedia nc rdacarrier Dresden, Techn. Univ., Diss., 2005 Zellzyklus - Saccharomycetaceae - Untereinheit - Ubiquitin-Protein-Ligase Untereinheit (DE-588)4335882-2 gnd rswk-swf Saccharomycetaceae (DE-588)4131614-9 gnd rswk-swf Ubiquitin-Protein-Ligase (DE-588)4398417-4 gnd rswk-swf Zellzyklus (DE-588)4129960-7 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Saccharomycetaceae (DE-588)4131614-9 s Zellzyklus (DE-588)4129960-7 s Ubiquitin-Protein-Ligase (DE-588)4398417-4 s Untereinheit (DE-588)4335882-2 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=015608061&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Schwickart, Martin Molecular analysis of novel subunits of the anaphasepromoting complex (APC/C) in yeast Zellzyklus - Saccharomycetaceae - Untereinheit - Ubiquitin-Protein-Ligase Untereinheit (DE-588)4335882-2 gnd Saccharomycetaceae (DE-588)4131614-9 gnd Ubiquitin-Protein-Ligase (DE-588)4398417-4 gnd Zellzyklus (DE-588)4129960-7 gnd |
| subject_GND | (DE-588)4335882-2 (DE-588)4131614-9 (DE-588)4398417-4 (DE-588)4129960-7 (DE-588)4113937-9 |
| title | Molecular analysis of novel subunits of the anaphasepromoting complex (APC/C) in yeast |
| title_auth | Molecular analysis of novel subunits of the anaphasepromoting complex (APC/C) in yeast |
| title_exact_search | Molecular analysis of novel subunits of the anaphasepromoting complex (APC/C) in yeast |
| title_exact_search_txtP | Molecular analysis of novel subunits of the anaphasepromoting complex (APC/C) in yeast |
| title_full | Molecular analysis of novel subunits of the anaphasepromoting complex (APC/C) in yeast von Martin Schwickart |
| title_fullStr | Molecular analysis of novel subunits of the anaphasepromoting complex (APC/C) in yeast von Martin Schwickart |
| title_full_unstemmed | Molecular analysis of novel subunits of the anaphasepromoting complex (APC/C) in yeast von Martin Schwickart |
| title_short | Molecular analysis of novel subunits of the anaphasepromoting complex (APC/C) in yeast |
| title_sort | molecular analysis of novel subunits of the anaphasepromoting complex apc c in yeast |
| topic | Zellzyklus - Saccharomycetaceae - Untereinheit - Ubiquitin-Protein-Ligase Untereinheit (DE-588)4335882-2 gnd Saccharomycetaceae (DE-588)4131614-9 gnd Ubiquitin-Protein-Ligase (DE-588)4398417-4 gnd Zellzyklus (DE-588)4129960-7 gnd |
| topic_facet | Zellzyklus - Saccharomycetaceae - Untereinheit - Ubiquitin-Protein-Ligase Untereinheit Saccharomycetaceae Ubiquitin-Protein-Ligase Zellzyklus Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=015608061&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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