Synthesis and evaluation of novel formats in molecular imprinting:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English |
| Veröffentlicht: |
2005
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XI, 215 Bl. Ill., graph. Darst. 30 cm |
Internformat
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| 100 | 1 | |a Titirici, Magdalena |d 1977- |e Verfasser |0 (DE-588)129915572 |4 aut | |
| 245 | 1 | 0 | |a Synthesis and evaluation of novel formats in molecular imprinting |c von: Maria-Magdalena Titirici |
| 264 | 1 | |c 2005 | |
| 300 | |a XI, 215 Bl. |b Ill., graph. Darst. |c 30 cm | ||
| 336 | |b txt |2 rdacontent | ||
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Datensatz im Suchindex
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CONTENTS
1
SUMMARY
1
2
INTRODUCTION
4
3
MOLECULAR
IMPRINTING-STATE
OF
ART
10
3.1
INTRODUCTION
TO
MOLECULAR
IMPRINTING
10
3.2
AN
HISTORICAL
PERSPECTIVE
OF
THE
DEVELOPMENT
OF
MOLECULAR
IMPRINTING
11
3.2.1
MOLECULAR
IMPRINTING
IN
SILICA
11
3.2.2
MOLECULAR
IMPRINTING
IN
ORGANIC
POLYMERS
13
3.3
METHODS
FOR
PREPARATION
OF
SPHERICAL MIP
BEADS
20
3.4.
OTHER
MIP
FORMATS
25
3.5
GRAFTING
TECHNIQUES
27
3.5.1
THE
"GRAFTING
TO"
APPROACH
28
3.5.2
THE
"GRAFTING
FROM"
APPROACH
30
3.6
SURFACE
OR INTERFACIAL
IMPRINTING
34
3.7
APPLICATIONS
OF
MOLECULARLY
IMPRINTED
POLYMERS
42
3.7.1
CHIRAL
SEPARATIONS
(MICSP)
42
3.7.2.
PEPTIDES
AND
PROTEINS
RECOGNITION
45
3.7.3
SOLID
PHASE
EXTRACTIONS
50
3.7.4
BIOSENSORS
51
3.7.5.
CATALYSIS
51
3.8
CONCLUSIONS
52
4
RESULTS
AND
DISCUSSION
(I):
HIERARCHICAL
IMPRINTING
54
4.1
IMPRINTING
USING
IMMOBILISED
NUCLEOTIDES
55
4.1.1
TEMPLATE
IMMOBILISATION
56
4.1.2
CHARACTERISATION
OF
THE
RESULTING
INTERMEDIATES
60
4.1.3
PREPARATION
OF
THE
IMPRINTED
POLYMERS
65
4.1.4
CHARACTERISATION
OF
THE
IMPRINTED
POLYMERS
67
4.1.5
EVALUATION
OF
THE
IMPRINTED
POLYMERS
AS
STATIONARY
PHASES
IN
HPLC
74
4.1.5.1
EVALUATION
OF
ADENINE IMPRINTED
POLYMER
PREPARED
USING
GPS
74
4.1.5.2
EVALUATION
OF
ADENINE
IMPRINTED
POLYMERS
PREPARED
USING
CPS
76
CONTENTS
4.1.5.3
EVALUATION
OF
9-ETHYADENINE
AND
TRIAMINOPYRIMIDINE
IMPRINTED
POLYMERS
PREPARED
USING
APS
77
4.1.5.4
COMPARISON
BETWEEN
DIFFERENT
SILANE
APPROACHES
79
4.1.5.5
BINDING
MEASUREMENTS
IN
AQUEOUS
MEDIA
81
4.1.5.6
RECOGNITION
OF
LARGER
MOLECULES
84
4.1.5.
7
COMPARISON
BETWEEN
HIERARCHICAL AND
BULK
POLYMERS
85
4.1.6
CONCLUSIONS
87
4.2
IMPRINTING
USING
IMMOBILISED
PEPTIDES
89
4.2.1
SOLID
PHASE
SYNTHESIS;
TEMPLATE
IMMOBILISATION
90
4.2.2
CHARACTERISATION
OF
THE
SOLID
PHASE
SYNTHESIS
PRODUCTS
95
4.2.3
PREPARATION
OF
THE
IMPRINTED
POLYMERS
97
4.2.4
CHARACTERISATION
OF
THE
IMPRINTED
POLYMERS
99
4.2.5
ASSESSMENT
OF
THE
IMPRINTED
POLYMERS
AS
STATIONARY
PHASES
IN
HPLC
106
4.2.5.1
INFLUENCE
OF
COVALENT
TEMPLATE
IMMOBILISATION
106
4.2.5.2
SELECTIVITY
OF
MONO
AND
DI-AMINO
ACIDS
EPITOPE
IMPRINTED
POLYMERS
TOWARDS
SMALL
AMINO
ACID
DERIVATIVES
108
4.2.5.3
SELECTIVITY
OF
MONO
AND
DI-AMINO
ACIDS
EPITOPE
IMPRINTED
POLYMERS
TOWARDS
LARGER
PEPTIDES
110
4.2.5.4
THE
EFFECT
OF
EPITOPE
SIZE
ON
RETENTION
TOWARDS
LARGER
PEPTIDES
112
4.2.6
CONCLUSIONS
115
4.3
IMPORTANCE
AND OUTLOOK
116
5
RESULTS
AND
DISCUSSION
(II):
THIN
FILM
MIP-COMPOSITES
119
5.1.
LIVING
RADICAL
POLYMERISATION
120
5.2
GRAFTING
OF
MIPS
VIA
A
SURFACE
BOUND
AZO-INITIATOR
USING
RAFT
POLYMERISATION
129
5.2.1
SURFACE
ATTACHMENT
OF
FREE
RADICAL
INITIATOR
129
5.2.2
GRAFTING
STEP
130
5.2.3
CHARACTERISATION
OF
GRAFTED
POLYMER
LAYERS
133
5.2.4
CHARACTERISATION
OF
THE
MATERIALS
IN
THE
CHROMATOGRAPHIC
MODE
140
5.2.5
CONCLUSIONS
AND
OUTLOOK
144
5.3
GRAFTING
OF
MOLECULARLY
IMPRINTED
POLYMERS
VIA
A
SURFACE
BOUND
INIFERTER
INITIATOR
145
5.3.1
INIFERTER
COUPLING
AND
CHARACTERISATION
145
5.3.2
POLYMER
GRAFTING
AND
COMPOSITE
CHARACTERISATION
146
5.3.3
EVALUATION
OF
THE
COMPOSITES
AS
STATIONARY
PHASES
IN
HPLC
153
VI
CONTENTS
5.3.4.
CONCLUSIONS
157
5.4
THIN-WALLED
IMPRINTED
POLYMERS
GENERATED
FROM
INIFERTER-BASED COMPOSITES
159
5.4.1
SYNTHESIS
AND
CHARACTERISATION
160
5.4.2
ENANTIOSELECTIVITY
OF
THIN-WALLED
MIPS
AND
CORRESPONDING
COMPOSITES
169
5.4.3
IMPORTANCE
AND
OUTLOOK
180
6
EXPERIMENTAL
SECTION
182
6.1
HIERARCHICAL
IMPRINTING
USING
IMMOBILISED
NUCLEOTIDES
182
6.1.1
SILICA
SURFACE
ACTIVATION
182
6.1.2
SILICA
SURFACE
SILANISATION
182
6.1.3
END-CAPPING
USING
HEXAMETHYLDISILAZANE
(HMDS)
183
6.1.4
IMMOBILISATION
OF
ADENINE
ONTO
THE
GPS-MODIFIED
SILICA
SURFACE
183
6.1.5
IMMOBILISATION
OF
ADENINE
ONTO
THE
CPS-MODIFIED
SILICA
SURFACE
183
6.1.6
IMMOBILISATION
OF
ADENINE
ONTO
THE
APS-MODIFIED
SILICA SURFACE
184
6.1.7
IMMOBILISATION
OF
PYRIMIDINE
ONTO
THE
APS-MODIFIED
SILICA
SURFACE
184
6.1.8
POLYMERISATION
IN
THE
TEMPLATE-MODIFIED
SILICA
PORES
185
6.1.9
SILICA
DISSOLUTION
FROM
THE
RESULTING
COMPOSITES
185
6.1.10
COUPLING
OF
FLUORESCENT
LABEL
186
6.2
HIERARCHICAL
IMPRINTING
USING
IMMOBILISED
PEPTIDES
186
6.2.1
IMMOBILISATION
OF
FMOC-GLY-OH
ONTO
THE
APS-MODIFIED
SILICA
SURFACE
186
6.2.2
DEPROTECTION
OF
FMOC-GLY-SI
187
6.2.3
COUPLING
REACTION
BETWEEN
FMOC-PHE-OH
AND
H-GLY-SI
187
6.2.4
DEPROTECTION
OF
FMOC-PHE-GLY-SI
188
6.3
THIN
LAYER
MIP-COMPOSITES
VIA
A
SURFACE
BOUND
AZO
INITIATOR
AND
RAFT
POLYMERISATION
188
6.3.1
ATTACHMENT
OF
AZO
BIS(CYANOPENTANOIC
ACID)
TO
THE
SILICA
SURFACE
188
6.3.2
RAFT
AGENT
SYNTHESIS
190
6.3.3
TEMPLATE
SYNTHESIS
190
6.3.3.
1
SYNTHESIS
OF
BOC-IJD-PHENYLALANINE
ANILIDE
191
VII
CONTENTS
63.3.2
SYNTHESIS
OFL/D-PHENYLALANINE
ANILIDE
191
6.3.4
POLYMERISATION
.192
6.4
THIN
LAYER
MIP-COMPOSITES
VIA
A
SURFACE
BOUND
INIFERTER-INITIATOR
193
6.4.1
SILANISATION
OF
SILICA
SURFACE
WITH
P-(CHLOROMETHYL)
PHENYL
TRIMETHOXYSILANE
193
6.4.2
IMMBOBILISATION
OF
BENZYL
N,
N-DIETHYLDITHIOCARBAMATE
INIFERTER
193
6.4.3
POLYMERISATION
194
6.4.4
GENERATION
OF
THIN-WALLED
MIPS
194
6.4.5
SYNTHESIS
OF
BENZYL
N,N-DIETHYLDITHIOCARBAMATE
INIFERTER
IN
SOLUTION
194
6.4.6.
TREATMENT
OF
BENZYL
N,N-DIETHYLDITHIOCARBAMATE
WITH
AMMONIUM
HYDROGEN
DIFLUORIDE
195
6.5
CHARACTERISATION
TECHNIQUES
AND
THEORY
195
6.5.1
ELEMENTAL
ANALYSIS
195
6.5.2
FT-IR
SPECTROSCOPY
195
6.5.3
FLUORESCENCE
MICROSCOPY
196
6.5.4.
THERMOGRAVIMETRIC
ANALYSIS
196
6.5.5
SCANNING ELECTRON
MICROSCOPY
196
6.5.6.
TRANSMISSION ELECTRON
MICROSCOPY
197
6.5.7.
ENERGY
DISPERSIVE
X-RAY
ANALYSIS
197
6.5.8.
SWELLING
EXPERIMENTS
197
6.5.9
NITROGEN
ADSORPTION
MEASUREMENTS
198
6.5.10
HPLC
MEASUREMENTS
.
200
6.5.11
REBINDING
TESTS
201
6.5.12
KINETIC
EXPERIMENTS
203
6.6
CHEMICALS
.
204
6.6.1
CHEMICALS
FOR
SYNTHESIS
'
204
6.6.2
CHEMICALS
FOR
ANALYSIS
.
206
6.6.3
HPLC
SOLVENTS
AND
CHEMICALS
206
7
REFERENCES
207
VIII |
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| id | DE-604.BV022391507 |
| illustrated | Illustrated |
| index_date | 2024-07-02T17:14:58Z |
| indexdate | 2024-11-22T17:42:28Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-015600333 |
| oclc_num | 633367698 |
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| physical | XI, 215 Bl. Ill., graph. Darst. 30 cm |
| publishDate | 2005 |
| publishDateSearch | 2005 |
| publishDateSort | 2005 |
| record_format | marc |
| spelling | Titirici, Magdalena 1977- Verfasser (DE-588)129915572 aut Synthesis and evaluation of novel formats in molecular imprinting von: Maria-Magdalena Titirici 2005 XI, 215 Bl. Ill., graph. Darst. 30 cm txt rdacontent n rdamedia nc rdacarrier Dortmund, Univ., Diss., 2005 Trennverfahren (DE-588)4078395-9 gnd rswk-swf Stationäre Phase (DE-588)4247829-7 gnd rswk-swf Mesoporöser Kristall (DE-588)4509028-2 gnd rswk-swf Polymere (DE-588)4046699-1 gnd rswk-swf Molekulares Prägen (DE-588)4589315-9 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Polymere (DE-588)4046699-1 s Mesoporöser Kristall (DE-588)4509028-2 s Molekulares Prägen (DE-588)4589315-9 s Stationäre Phase (DE-588)4247829-7 s Trennverfahren (DE-588)4078395-9 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=015600333&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Titirici, Magdalena 1977- Synthesis and evaluation of novel formats in molecular imprinting Trennverfahren (DE-588)4078395-9 gnd Stationäre Phase (DE-588)4247829-7 gnd Mesoporöser Kristall (DE-588)4509028-2 gnd Polymere (DE-588)4046699-1 gnd Molekulares Prägen (DE-588)4589315-9 gnd |
| subject_GND | (DE-588)4078395-9 (DE-588)4247829-7 (DE-588)4509028-2 (DE-588)4046699-1 (DE-588)4589315-9 (DE-588)4113937-9 |
| title | Synthesis and evaluation of novel formats in molecular imprinting |
| title_auth | Synthesis and evaluation of novel formats in molecular imprinting |
| title_exact_search | Synthesis and evaluation of novel formats in molecular imprinting |
| title_exact_search_txtP | Synthesis and evaluation of novel formats in molecular imprinting |
| title_full | Synthesis and evaluation of novel formats in molecular imprinting von: Maria-Magdalena Titirici |
| title_fullStr | Synthesis and evaluation of novel formats in molecular imprinting von: Maria-Magdalena Titirici |
| title_full_unstemmed | Synthesis and evaluation of novel formats in molecular imprinting von: Maria-Magdalena Titirici |
| title_short | Synthesis and evaluation of novel formats in molecular imprinting |
| title_sort | synthesis and evaluation of novel formats in molecular imprinting |
| topic | Trennverfahren (DE-588)4078395-9 gnd Stationäre Phase (DE-588)4247829-7 gnd Mesoporöser Kristall (DE-588)4509028-2 gnd Polymere (DE-588)4046699-1 gnd Molekulares Prägen (DE-588)4589315-9 gnd |
| topic_facet | Trennverfahren Stationäre Phase Mesoporöser Kristall Polymere Molekulares Prägen Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=015600333&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT titiricimagdalena synthesisandevaluationofnovelformatsinmolecularimprinting |