Microarray technology and cancer gene profiling:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
New York, NY
Springer Science+Business Media [u.a.]
2007
|
| Schriftenreihe: | Advances in experimental medicine and biology
593 |
| Schlagworte: | |
| Online-Zugang: | Inhaltstext Inhaltsverzeichnis |
| Beschreibung: | XVIII, 159 S. Ill., graph. Darst. |
| ISBN: | 9780387399775 0387399771 |
Internformat
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Datensatz im Suchindex
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CONTENTS
SECTION I: MICROARRAY TECHNOLOGY
1.
David W. Petersen and Ernest S. Kawasaki
Abstract
Introduction
Nomenclature.
The
Robotic Printers
Printing Pins
Microarray Slide Substrates
Personnel
Conclusions
2.
GENE EXPRESSION PROFILING
Susanna Mandruzzato
Abstract
Principles of Gene Microarray Technology
Target Preparation and Hybridization
Image Acquisition and Quantification
3.
Simone Mocellin
Abstract
Introduction
Study Design and Data Analysis
Data Collection and Normalization
Comparing Expression Data
xvi Contents
4.
ABOUTIT!
Ernst Wit,
Abstract
A Light Introduction
P-Value Weighting
Within-Spot Pixel Variance Weighting
A Weighty Discussion
5.1.
RNA
Ena
Abstract
Introduction
Source Material Collection and
Single-Strand cDNA Synthesis
Double-Stranded cDNA (ds-cDNA) Synthesis
RNA
Target Labeling for cDNA Microarray Using Amplified
Appendix:
5.2.
LASER CAPTURE.
INCREASING SPECIFICITY OF GENE EXPRESSION
PROFILING OF CANCER SPECIMENS
Giovanni Esposito
Abstract
Microdissection
Why
Slide Preparation for
In Our Laboratory
Conclusions
53.
VALIDATION OF GENE EXPRESSION DATA
BY QUANTITATIVE REAL TIME PCR
Maurizio Provenzano
Abstract
PCR-Based Analysis of Gene Quantitation
Principles
Main Issues
Messenger
Contents xvii
SECTION
6. MICROARRAYS
AND CLASSIFICATION
Ainhoa Perez-Diez,
Abstract
Introduction
Revealing Expression Profiles for Cancer Diagnosis
and Classification
Using Expression Profiles in the Clinic
Perspective
7.
OF
THE CASE OF IMMUNOTHERAPY
Vladia
Experimental Models, Human Polymorphisms
and Cancer Heterogeneity
Gene Profiling and T-Cell Phenotypes
Interactions between Tumor Cells and TA-Specific
The Tumor Microenvironment
Influence of Genetic Background and Cancer Heterogeneity
on Immune Responsiveness
Gene Profiling of
Conclusions
8.
OF TUMOR SENSITIVITY AND RESISTANCE
TO
Luigi
Abstract
Introduction
Correlation of Gene Expression Data from
with Response to
Conclusions
9.
Lu Wang,
Abstract
Introduction
Enzymatic Approach
xviii Contents
Polymerase Chain
Microarray
Invader Assay.
Mass Spectrometry
Sequencing
Summary
10.
Mei
Abstract
Cancer Development
Science/Technology
Complementary Approach
In Vitro/PrecUnical Research Using Microarray
Clinical Studies
11.
LYMPHOMAS
Sarah E. Henrickson, Elena
and Andreas
Abstract
Introduction
Gene Expression Profiling
Gene Expression Analysis
The Applications of Gene Expression Profiling to Leukemia
and Lymphoma
12.
Simone Mocellin,
Abstract
Tumor Immunology and the Post-Genomic Era
Tumor Immune Escape
New Targets for Tumor Immunotherapy
Dendritic Cell Biology
Τ
Tumor
Concluding Remarks
INDEX
CONTENTS
SECTION I: MICROARRAY TECHNOLOGY
1.
David W. Petersen and Ernest S. Kawasaki
Abstract
Introduction
Nomenclature.
The
Robotic Printers
Printing Pins
Microarray Slide Substrates
Personnel
Conclusions
2.
GENE EXPRESSION PROFILING
Susanna Mandruzzato
Abstract
Principles of Gene Microarray Technology
Target Preparation and Hybridization
Image Acquisition and Quantification
3.
Simone Mocellin
Abstract
Introduction
Study Design and Data Analysis
Data Collection and Normalization
Comparing Expression Data
xvi Contents
4.
ABOUTIT!
Ernst Wit,
Abstract
A Light Introduction
P-Value Weighting
Within-Spot Pixel Variance Weighting
A Weighty Discussion
5.1.
RNA
Ena
Abstract
Introduction
Source Material Collection and
Single-Strand cDNA Synthesis
Double-Stranded cDNA (ds-cDNA) Synthesis
RNA
Target Labeling for cDNA Microarray Using Amplified
Appendix:
5.2.
LASER CAPTURE.
INCREASING SPECIFICITY OF GENE EXPRESSION
PROFILING OF CANCER SPECIMENS
Giovanni Esposito
Abstract
Microdissection
Why
Slide Preparation for
In Our Laboratory
Conclusions
53.
VALIDATION OF GENE EXPRESSION DATA
BY QUANTITATIVE REAL TIME PCR
Maurizio Provenzano
Abstract
PCR-Based Analysis of Gene Quantitation
Principles
Main Issues
Messenger
Contents xvii
SECTION
6. MICROARRAYS
AND CLASSIFICATION
Ainhoa Perez-Diez,
Abstract
Introduction
Revealing Expression Profiles for Cancer Diagnosis
and Classification
Using Expression Profiles in the Clinic
Perspective
7.
OF
THE CASE OF IMMUNOTHERAPY
Vladia
Experimental Models, Human Polymorphisms
and Cancer Heterogeneity
Gene Profiling and T-Cell Phenotypes
Interactions between Tumor Cells and TA-Specific
The Tumor Microenvironment
Influence of Genetic Background and Cancer Heterogeneity
on Immune Responsiveness
Gene Profiling of
Conclusions
8.
OF TUMOR SENSITIVITY AND RESISTANCE
TO
Luigi
Abstract
Introduction
Correlation of Gene Expression Data from
with Response to
Conclusions
9.
Lu Wang,
Abstract
Introduction
Enzymatic Approach
xviii Contents
Polymerase Chain
Microarray
Invader Assay.
Mass Spectrometry
Sequencing
Summary
10.
Mei
Abstract
Cancer Development
Science/Technology
Complementary Approach
In Vitro/PrecUnical Research Using Microarray
Clinical Studies
11.
LYMPHOMAS
Sarah E. Henrickson, Elena
and Andreas
Abstract
Introduction
Gene Expression Profiling
Gene Expression Analysis
The Applications of Gene Expression Profiling to Leukemia
and Lymphoma
12.
Simone Mocellin,
Abstract
Tumor Immunology and the Post-Genomic Era
Tumor Immune Escape
New Targets for Tumor Immunotherapy
Dendritic Cell Biology
Τ
Tumor
Concluding Remarks
INDEX |
| adam_txt |
CONTENTS
SECTION I: MICROARRAY TECHNOLOGY
1.
David W. Petersen and Ernest S. Kawasaki
Abstract
Introduction
Nomenclature.
The
Robotic Printers
Printing Pins
Microarray Slide Substrates
Personnel
Conclusions
2.
GENE EXPRESSION PROFILING
Susanna Mandruzzato
Abstract
Principles of Gene Microarray Technology
Target Preparation and Hybridization
Image Acquisition and Quantification
3.
Simone Mocellin
Abstract
Introduction
Study Design and Data Analysis
Data Collection and Normalization
Comparing Expression Data
xvi Contents
4.
ABOUTIT!
Ernst Wit,
Abstract
A Light Introduction
P-Value Weighting
Within-Spot Pixel Variance Weighting
A Weighty Discussion
5.1.
RNA
Ena
Abstract
Introduction
Source Material Collection and
Single-Strand cDNA Synthesis
Double-Stranded cDNA (ds-cDNA) Synthesis
RNA
Target Labeling for cDNA Microarray Using Amplified
Appendix:
5.2.
LASER CAPTURE.
INCREASING SPECIFICITY OF GENE EXPRESSION
PROFILING OF CANCER SPECIMENS
Giovanni Esposito
Abstract
Microdissection
Why
Slide Preparation for
In Our Laboratory
Conclusions
53.
VALIDATION OF GENE EXPRESSION DATA
BY QUANTITATIVE REAL TIME PCR
Maurizio Provenzano
Abstract
PCR-Based Analysis of Gene Quantitation
Principles
Main Issues
Messenger
Contents xvii
SECTION
6. MICROARRAYS
AND CLASSIFICATION
Ainhoa Perez-Diez,
Abstract
Introduction
Revealing Expression Profiles for Cancer Diagnosis
and Classification
Using Expression Profiles in the Clinic
Perspective
7.
OF
THE CASE OF IMMUNOTHERAPY
Vladia
Experimental Models, Human Polymorphisms
and Cancer Heterogeneity
Gene Profiling and T-Cell Phenotypes
Interactions between Tumor Cells and TA-Specific
The Tumor Microenvironment
Influence of Genetic Background and Cancer Heterogeneity
on Immune Responsiveness
Gene Profiling of
Conclusions
8.
OF TUMOR SENSITIVITY AND RESISTANCE
TO
Luigi
Abstract
Introduction
Correlation of Gene Expression Data from
with Response to
Conclusions
9.
Lu Wang,
Abstract
Introduction
Enzymatic Approach
xviii Contents
Polymerase Chain
Microarray
Invader Assay.
Mass Spectrometry
Sequencing
Summary
10.
Mei
Abstract
Cancer Development
Science/Technology
Complementary Approach
In Vitro/PrecUnical Research Using Microarray
Clinical Studies
11.
LYMPHOMAS
Sarah E. Henrickson, Elena
and Andreas
Abstract
Introduction
Gene Expression Profiling
Gene Expression Analysis
The Applications of Gene Expression Profiling to Leukemia
and Lymphoma
12.
Simone Mocellin,
Abstract
Tumor Immunology and the Post-Genomic Era
Tumor Immune Escape
New Targets for Tumor Immunotherapy
Dendritic Cell Biology
Τ
Tumor
Concluding Remarks
INDEX
CONTENTS
SECTION I: MICROARRAY TECHNOLOGY
1.
David W. Petersen and Ernest S. Kawasaki
Abstract
Introduction
Nomenclature.
The
Robotic Printers
Printing Pins
Microarray Slide Substrates
Personnel
Conclusions
2.
GENE EXPRESSION PROFILING
Susanna Mandruzzato
Abstract
Principles of Gene Microarray Technology
Target Preparation and Hybridization
Image Acquisition and Quantification
3.
Simone Mocellin
Abstract
Introduction
Study Design and Data Analysis
Data Collection and Normalization
Comparing Expression Data
xvi Contents
4.
ABOUTIT!
Ernst Wit,
Abstract
A Light Introduction
P-Value Weighting
Within-Spot Pixel Variance Weighting
A Weighty Discussion
5.1.
RNA
Ena
Abstract
Introduction
Source Material Collection and
Single-Strand cDNA Synthesis
Double-Stranded cDNA (ds-cDNA) Synthesis
RNA
Target Labeling for cDNA Microarray Using Amplified
Appendix:
5.2.
LASER CAPTURE.
INCREASING SPECIFICITY OF GENE EXPRESSION
PROFILING OF CANCER SPECIMENS
Giovanni Esposito
Abstract
Microdissection
Why
Slide Preparation for
In Our Laboratory
Conclusions
53.
VALIDATION OF GENE EXPRESSION DATA
BY QUANTITATIVE REAL TIME PCR
Maurizio Provenzano
Abstract
PCR-Based Analysis of Gene Quantitation
Principles
Main Issues
Messenger
Contents xvii
SECTION
6. MICROARRAYS
AND CLASSIFICATION
Ainhoa Perez-Diez,
Abstract
Introduction
Revealing Expression Profiles for Cancer Diagnosis
and Classification
Using Expression Profiles in the Clinic
Perspective
7.
OF
THE CASE OF IMMUNOTHERAPY
Vladia
Experimental Models, Human Polymorphisms
and Cancer Heterogeneity
Gene Profiling and T-Cell Phenotypes
Interactions between Tumor Cells and TA-Specific
The Tumor Microenvironment
Influence of Genetic Background and Cancer Heterogeneity
on Immune Responsiveness
Gene Profiling of
Conclusions
8.
OF TUMOR SENSITIVITY AND RESISTANCE
TO
Luigi
Abstract
Introduction
Correlation of Gene Expression Data from
with Response to
Conclusions
9.
Lu Wang,
Abstract
Introduction
Enzymatic Approach
xviii Contents
Polymerase Chain
Microarray
Invader Assay.
Mass Spectrometry
Sequencing
Summary
10.
Mei
Abstract
Cancer Development
Science/Technology
Complementary Approach
In Vitro/PrecUnical Research Using Microarray
Clinical Studies
11.
LYMPHOMAS
Sarah E. Henrickson, Elena
and Andreas
Abstract
Introduction
Gene Expression Profiling
Gene Expression Analysis
The Applications of Gene Expression Profiling to Leukemia
and Lymphoma
12.
Simone Mocellin,
Abstract
Tumor Immunology and the Post-Genomic Era
Tumor Immune Escape
New Targets for Tumor Immunotherapy
Dendritic Cell Biology
Τ
Tumor
Concluding Remarks
INDEX |
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| owner | DE-355 DE-BY-UBR DE-12 |
| owner_facet | DE-355 DE-BY-UBR DE-12 |
| physical | XVIII, 159 S. Ill., graph. Darst. |
| publishDate | 2007 |
| publishDateSearch | 2007 |
| publishDateSort | 2007 |
| publisher | Springer Science+Business Media [u.a.] |
| record_format | marc |
| series | Advances in experimental medicine and biology |
| series2 | Advances in experimental medicine and biology |
| spelling | Microarray technology and cancer gene profiling ed. by Simone Mocellin New York, NY Springer Science+Business Media [u.a.] 2007 XVIII, 159 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Advances in experimental medicine and biology 593 Oncogènes Puces à ADN DNA microarrays Gene Expression Profiling methods Microarray Analysis methods Neoplasms genetics Oncogenes Tumorzelle (DE-588)4186433-5 gnd rswk-swf Onkogen (DE-588)4131118-8 gnd rswk-swf Microarray (DE-588)4544227-7 gnd rswk-swf Genexpression (DE-588)4020136-3 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Tumorzelle (DE-588)4186433-5 s Onkogen (DE-588)4131118-8 s Genexpression (DE-588)4020136-3 s Microarray (DE-588)4544227-7 s b DE-604 Mocellin, Simone Sonstige oth Advances in experimental medicine and biology 593 (DE-604)BV000003102 593 text/html http://deposit.dnb.de/cgi-bin/dokserv?id=2845234&prov=M&dok_var=1&dok_ext=htm Inhaltstext Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=015506142&sequence=000005&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Microarray technology and cancer gene profiling Advances in experimental medicine and biology Oncogènes Puces à ADN DNA microarrays Gene Expression Profiling methods Microarray Analysis methods Neoplasms genetics Oncogenes Tumorzelle (DE-588)4186433-5 gnd Onkogen (DE-588)4131118-8 gnd Microarray (DE-588)4544227-7 gnd Genexpression (DE-588)4020136-3 gnd |
| subject_GND | (DE-588)4186433-5 (DE-588)4131118-8 (DE-588)4544227-7 (DE-588)4020136-3 (DE-588)4143413-4 |
| title | Microarray technology and cancer gene profiling |
| title_auth | Microarray technology and cancer gene profiling |
| title_exact_search | Microarray technology and cancer gene profiling |
| title_exact_search_txtP | Microarray technology and cancer gene profiling |
| title_full | Microarray technology and cancer gene profiling ed. by Simone Mocellin |
| title_fullStr | Microarray technology and cancer gene profiling ed. by Simone Mocellin |
| title_full_unstemmed | Microarray technology and cancer gene profiling ed. by Simone Mocellin |
| title_short | Microarray technology and cancer gene profiling |
| title_sort | microarray technology and cancer gene profiling |
| topic | Oncogènes Puces à ADN DNA microarrays Gene Expression Profiling methods Microarray Analysis methods Neoplasms genetics Oncogenes Tumorzelle (DE-588)4186433-5 gnd Onkogen (DE-588)4131118-8 gnd Microarray (DE-588)4544227-7 gnd Genexpression (DE-588)4020136-3 gnd |
| topic_facet | Oncogènes Puces à ADN DNA microarrays Gene Expression Profiling methods Microarray Analysis methods Neoplasms genetics Oncogenes Tumorzelle Onkogen Microarray Genexpression Aufsatzsammlung |
| url | http://deposit.dnb.de/cgi-bin/dokserv?id=2845234&prov=M&dok_var=1&dok_ext=htm http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=015506142&sequence=000005&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV000003102 |
| work_keys_str_mv | AT mocellinsimone microarraytechnologyandcancergeneprofiling |