Adenovirus methods and protocols: 2 Ad proteins, RNA, lifecycle, host interactions, and phylogenetics
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2007
|
| Ausgabe: | 2. ed. |
| Schriftenreihe: | Methods in molecular medicine
131 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis Klappentext |
| Beschreibung: | XIII, 362 S. Ill., graph. Darst. |
| ISBN: | 9781588299017 1588299015 |
Internformat
MARC
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Datensatz im Suchindex
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| adam_text | Adenoviras Methods and Protocols
Second Edition, Volume
Ad Proteins,
Edited by
William S. M. Wold and Ann E. Tollefson
Department of Molecular Microbiology and Immunology,
Saint Louis University School of Medicine, St Louis, Missouri
Adenovirus Methods and Protocols, Second Edition, now in two volumes, is an
essential resource for adenovirus (Ad) researchers beginning in the field, and an
inspirational starting point for researchers looking to branch into new areas of
Ad study. In addition to updating and expanding important chapters from the first
edition, the authors have added new chapters that address innovative, exciting areas
of emphasis in Ad research, including Ad vector construction and use, real-time PCR,
use of new animal models, and methods for quantification of Ad virus or virus
expression/interactions. Each of the protocols presented in these volumes is written
by trendsetting researchers in their respective areas of expertise.
In this volume, the authors focus on methods that elucidate and quantitate the
interactions of Ad with the host. Each of the protocols in these volumes provides a
general introduction,followed bytried-and-true step-by-step methods. Both novice
and experienced researchers will reap tremendous benefit from these groundbreak¬
ing volumes in Ad research. Volume
construction of adenoviruses for use as vectors and for basic research. Highlighted
topics include deletion mutants, capsid modifications, insertions, and gene replace¬
ments in human,
Features
Detailed description of quality
control testing of vectors
Methods for determination
of virus titers
Sensitive assays for testing viral
DNA
Developing new, permissive
immunocompetent animal models
Characterization of specific
Ad proteins (hexon, fiber, or
protease/proteinase)
Procedures for
transformation assays, and NK
cell-mediated cytolysis
Analysis of phylogenetics and
genetic variability
Contents
Preface
Contents
Contributors
1
Cristina
2
of Viral Protein Domains
Paul M. Loewenstein, Chao-Zhong Song, and Maurice Green
3
for Studies of
Oliver
4
an Adenovirus Infection
Gunnar Andersson, Ning
5
by Fluorescent Labeling In Situ
Eileen Bridge
6
David A. Matthews
7
David A. Omettes,
and Felicia D. Goodrum
8
Peter Yaciuk
9
to the Adenovirus Genome In Vivo
Jihong Yang and Patrick Hearing
10
Chromatin Immunoprecipitation and Electrophoretic Mobility
Shift Assays
Pilar
11
Tandem Mass Spectrometry
Anuj
vii
viii Contents
12
Transcriptional Regulatory Protein Domains
Maurice Green, Andrew Thorburn, Robert Kern,
and Paul M. Loewenstein
13
of Adenovirus Oncogenes
Michael
14
to Immortalize the Viral Genome
Walter
15
Infected or Adenovirus-Transformed Cells
John M. Routes
16
Cell-Mediated Cytolysis of Adenovirus-Transformed Cells
Graham Bottley, Graham P. Cook, and G. Eric Blair
17
John J. Rux and Roger M. Burnett
18
Joseph M. Weber
19
and Synthesis of a Fluorogenic Substrate
Walter F. Mangel and William J. McGrath
20
Dissociation Constants and Stoichiometries of Binding
Walter F. Mangel and William J. McGrath
21
Jeffrey A. Engler and Jeong Shin Hong
22
Balázs
23
Adenoviruses for Molecular Epidemiology Studies
Adriana
Index
|
| adam_txt |
Adenoviras Methods and Protocols
Second Edition, Volume
Ad Proteins,
Edited by
William S. M. Wold and Ann E. Tollefson
Department of Molecular Microbiology and Immunology,
Saint Louis University School of Medicine, St Louis, Missouri
Adenovirus Methods and Protocols, Second Edition, now in two volumes, is an
essential resource for adenovirus (Ad) researchers beginning in the field, and an
inspirational starting point for researchers looking to branch into new areas of
Ad study. In addition to updating and expanding important chapters from the first
edition, the authors have added new chapters that address innovative, exciting areas
of emphasis in Ad research, including Ad vector construction and use, real-time PCR,
use of new animal models, and methods for quantification of Ad virus or virus
expression/interactions. Each of the protocols presented in these volumes is written
by trendsetting researchers in their respective areas of expertise.
In this volume, the authors focus on methods that elucidate and quantitate the
interactions of Ad with the host. Each of the protocols in these volumes provides a
general introduction,followed bytried-and-true step-by-step methods. Both novice
and experienced researchers will reap tremendous benefit from these groundbreak¬
ing volumes in Ad research. Volume
construction of adenoviruses for use as vectors and for basic research. Highlighted
topics include deletion mutants, capsid modifications, insertions, and gene replace¬
ments in human,
Features
Detailed description of quality
control testing of vectors
Methods for determination
of virus titers
Sensitive assays for testing viral
DNA
Developing new, permissive
immunocompetent animal models
Characterization of specific
Ad proteins (hexon, fiber, or
protease/proteinase)
Procedures for
transformation assays, and NK
cell-mediated cytolysis
Analysis of phylogenetics and
genetic variability
Contents
Preface
Contents
Contributors
1
Cristina
2
of Viral Protein Domains
Paul M. Loewenstein, Chao-Zhong Song, and Maurice Green
3
for Studies of
Oliver
4
an Adenovirus Infection
Gunnar Andersson, Ning
5
by Fluorescent Labeling In Situ
Eileen Bridge
6
David A. Matthews
7
David A. Omettes,
and Felicia D. Goodrum
8
Peter Yaciuk
9
to the Adenovirus Genome In Vivo
Jihong Yang and Patrick Hearing
10
Chromatin Immunoprecipitation and Electrophoretic Mobility
Shift Assays
Pilar
11
Tandem Mass Spectrometry
Anuj
vii
viii Contents
12
Transcriptional Regulatory Protein Domains
Maurice Green, Andrew Thorburn, Robert Kern,
and Paul M. Loewenstein
13
of Adenovirus Oncogenes
Michael
14
to Immortalize the Viral Genome
Walter
15
Infected or Adenovirus-Transformed Cells
John M. Routes
16
Cell-Mediated Cytolysis of Adenovirus-Transformed Cells
Graham Bottley, Graham P. Cook, and G. Eric Blair
17
John J. Rux and Roger M. Burnett
18
Joseph M. Weber
19
and Synthesis of a Fluorogenic Substrate
Walter F. Mangel and William J. McGrath
20
Dissociation Constants and Stoichiometries of Binding
Walter F. Mangel and William J. McGrath
21
Jeffrey A. Engler and Jeong Shin Hong
22
Balázs
23
Adenoviruses for Molecular Epidemiology Studies
Adriana
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| index_date | 2024-07-02T16:49:44Z |
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| isbn | 9781588299017 1588299015 |
| language | English |
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| physical | XIII, 362 S. Ill., graph. Darst. |
| publishDate | 2007 |
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| publisher | Humana Press |
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| series | Methods in molecular medicine |
| series2 | Methods in molecular medicine |
| spelling | Adenovirus methods and protocols 2 Ad proteins, RNA, lifecycle, host interactions, and phylogenetics ed. by William S. M. Wold ... 2. ed. Totowa, NJ Humana Press 2007 XIII, 362 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular medicine 131 Methods in molecular medicine ... Adenoviridae Adenoviridae ram Adenoviridae laboratory manuals Adenoviren (DE-588)4141412-3 gnd rswk-swf Molekulare Medizin (DE-588)4543844-4 gnd rswk-swf Adenoviren (DE-588)4141412-3 s Molekulare Medizin (DE-588)4543844-4 s DE-604 Wold, William S. M. Sonstige oth (DE-604)BV022282157 2 Methods in molecular medicine 131 (DE-604)BV011334960 131 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=015492457&sequence=000003&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=015492457&sequence=000004&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA Klappentext |
| spellingShingle | Adenovirus methods and protocols Methods in molecular medicine Adenoviridae Adenoviridae ram Adenoviridae laboratory manuals Adenoviren (DE-588)4141412-3 gnd Molekulare Medizin (DE-588)4543844-4 gnd |
| subject_GND | (DE-588)4141412-3 (DE-588)4543844-4 |
| title | Adenovirus methods and protocols |
| title_auth | Adenovirus methods and protocols |
| title_exact_search | Adenovirus methods and protocols |
| title_exact_search_txtP | Adenovirus methods and protocols |
| title_full | Adenovirus methods and protocols 2 Ad proteins, RNA, lifecycle, host interactions, and phylogenetics ed. by William S. M. Wold ... |
| title_fullStr | Adenovirus methods and protocols 2 Ad proteins, RNA, lifecycle, host interactions, and phylogenetics ed. by William S. M. Wold ... |
| title_full_unstemmed | Adenovirus methods and protocols 2 Ad proteins, RNA, lifecycle, host interactions, and phylogenetics ed. by William S. M. Wold ... |
| title_short | Adenovirus methods and protocols |
| title_sort | adenovirus methods and protocols ad proteins rna lifecycle host interactions and phylogenetics |
| topic | Adenoviridae Adenoviridae ram Adenoviridae laboratory manuals Adenoviren (DE-588)4141412-3 gnd Molekulare Medizin (DE-588)4543844-4 gnd |
| topic_facet | Adenoviridae Adenoviridae laboratory manuals Adenoviren Molekulare Medizin |
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