Verfügbarkeit: Protein purification

Protein purification: principles, high resolution methods, and applications

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Bibliographische Detailangaben
Weitere Verfasser:	Janson, Jan-Christer (HerausgeberIn)
Format:	Buch
Sprache:	English
Veröffentlicht:	Hoboken, NJ Wiley 2011
Ausgabe:	3. ed.
Schriftenreihe:	Methods of biochemical analysis 54
Schlagworte:	Chromatographie Trennverfahren Elektrophorese Reinigung Proteine Aufsatzsammlung
Online-Zugang:	Inhaltsverzeichnis
Beschreibung:	XIV, 517 S. Ill., graph. Darst.
ISBN:	0471746614 9780471746614

Internformat

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Datensatz im Suchindex

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adam_text	CONTENTS PREFACE TO THE THIRD EDITION vii PREFACE TO THE SECOND EDITION ix PREFACE TO THE FIRST EDITION xi CONTRIBUTORS xiii PART I INTRODUCTION l 1 Introduction to Protein Purification 3 Bo Ersson, Lars Rydén, and Jan-Christer Janson PART II CHROMATOGRAPHY 23 2 Introduction to Chromatography 25 Jan-Christer Janson and Jan Ake Jönsson 3 Gel Filtration: Size Exclusion Chromatography 51 Lars Hagel 4 Ion Exchange Chromatography 93 Evert Karlsson and Irwin Hirsh 5 High-Resolution Reversed-Phase Chromatography of Proteins 135 Sylvia Winkel Pettersson 6 Hydrophobie Interaction Chromatography 165 Kjell-Ove Eriksson and Makonnen Belew 7 Immobilized Metal Ion Affinity Chromatography 183 Lennart Kågedal 8 Covalent Chromatography 203 Francisco Batista-Viera, Lars Rydén, and Jan Carlsson 9 Affinity Chromatography 221 Francisco Batista-Viera, Jan-Christer Janson, and Jan Carlsson vi CONTENTS 10 Affinity Ligands from Chemical Combinatorial Libraries 259 Enrique Corredano and Herbert Baumann 11 Affinity Ligands from Biological Combinatorial Libraries 269 Per-Ake Nygren PART III OTHER SEPARATION METHODS AND RELATED TECHNIQUES 279 12 Membrane Separations 281 Joachim K. Walter, Zuwei Jin, Maik W. Jomitz, and Uwe Gottschalk 13 Refolding of Inclusion Body Proteins from E. coli 319 Zhiguo Su, Diannan Lu, and Zheng Liu 14 Purification of PEGylated Proteins 339 Conan J. Fee and James M. Van Alstine PART IV ELECTROPHORESIS 363 15 Electrophoresis in Gels 365 Reiner Westermeier 16 Conventional Isoelectric Focusing in Gel Slabs and Capillaries and Immobilized pH Gradients 379 Pier Giorgio Righetti, Elisa Fasoli, and Sabina Carla Righetti 17 Two-Dimensional Electrophoresis in Proteomics 411 Reiner Westenneier und Angelika Görg 18 Protein Elution and Blotting Techniques 441 Reiner Westermeier 19 Capillary Electrophoretic Separations 451 Wolfgang Thorrmum PART V SEPARATION METHOD OPTIMIZATION 487 20 High Throughput Screening Techniques in Protein Purification 489 Karol M. Lacki and Eggert Brekkan INDEX 507
adam_txt	CONTENTS PREFACE TO THE THIRD EDITION vii PREFACE TO THE SECOND EDITION ix PREFACE TO THE FIRST EDITION xi CONTRIBUTORS xiii PART I INTRODUCTION l 1 Introduction to Protein Purification 3 Bo Ersson, Lars Rydén, and Jan-Christer Janson PART II CHROMATOGRAPHY 23 2 Introduction to Chromatography 25 Jan-Christer Janson and Jan Ake Jönsson 3 Gel Filtration: Size Exclusion Chromatography 51 Lars Hagel 4 Ion Exchange Chromatography 93 Evert Karlsson and Irwin Hirsh 5 High-Resolution Reversed-Phase Chromatography of Proteins 135 Sylvia Winkel Pettersson 6 Hydrophobie Interaction Chromatography 165 Kjell-Ove Eriksson and Makonnen Belew 7 Immobilized Metal Ion Affinity Chromatography 183 Lennart Kågedal 8 Covalent Chromatography 203 Francisco Batista-Viera, Lars Rydén, and Jan Carlsson 9 Affinity Chromatography 221 Francisco Batista-Viera, Jan-Christer Janson, and Jan Carlsson vi CONTENTS 10 Affinity Ligands from Chemical Combinatorial Libraries 259 Enrique Corredano and Herbert Baumann 11 Affinity Ligands from Biological Combinatorial Libraries 269 Per-Ake Nygren PART III OTHER SEPARATION METHODS AND RELATED TECHNIQUES 279 12 Membrane Separations 281 Joachim K. Walter, Zuwei Jin, Maik W. Jomitz, and Uwe Gottschalk 13 Refolding of Inclusion Body Proteins from E. coli 319 Zhiguo Su, Diannan Lu, and Zheng Liu 14 Purification of PEGylated Proteins 339 Conan J. Fee and James M. Van Alstine PART IV ELECTROPHORESIS 363 15 Electrophoresis in Gels 365 Reiner Westermeier 16 Conventional Isoelectric Focusing in Gel Slabs and Capillaries and Immobilized pH Gradients 379 Pier Giorgio Righetti, Elisa Fasoli, and Sabina Carla Righetti 17 Two-Dimensional Electrophoresis in Proteomics 411 Reiner Westenneier und Angelika Görg 18 Protein Elution and Blotting Techniques 441 Reiner Westermeier 19 Capillary Electrophoretic Separations 451 Wolfgang Thorrmum PART V SEPARATION METHOD OPTIMIZATION 487 20 High Throughput Screening Techniques in Protein Purification 489 Karol M. Lacki and Eggert Brekkan INDEX 507
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spelling	Protein purification principles, high resolution methods, and applications ed. by Jan-Christer Janson 3. ed. Hoboken, NJ Wiley 2011 XIV, 517 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods of biochemical analysis 54 Chromatographie (DE-588)4010153-8 gnd rswk-swf Trennverfahren (DE-588)4078395-9 gnd rswk-swf Elektrophorese (DE-588)4014373-9 gnd rswk-swf Reinigung (DE-588)4049250-3 gnd rswk-swf Proteine (DE-588)4076388-2 gnd rswk-swf 1\p (DE-588)4143413-4 Aufsatzsammlung gnd-content Proteine (DE-588)4076388-2 s Chromatographie (DE-588)4010153-8 s DE-604 Elektrophorese (DE-588)4014373-9 s Reinigung (DE-588)4049250-3 s Trennverfahren (DE-588)4078395-9 s Janson, Jan-Christer edt Methods of biochemical analysis 54 (DE-604)BV002528856 54 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=015489895&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk
spellingShingle	Protein purification principles, high resolution methods, and applications Methods of biochemical analysis Chromatographie (DE-588)4010153-8 gnd Trennverfahren (DE-588)4078395-9 gnd Elektrophorese (DE-588)4014373-9 gnd Reinigung (DE-588)4049250-3 gnd Proteine (DE-588)4076388-2 gnd
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title	Protein purification principles, high resolution methods, and applications
title_auth	Protein purification principles, high resolution methods, and applications
title_exact_search	Protein purification principles, high resolution methods, and applications
title_exact_search_txtP	Protein purification principles, high resolution methods, and applications
title_full	Protein purification principles, high resolution methods, and applications ed. by Jan-Christer Janson
title_fullStr	Protein purification principles, high resolution methods, and applications ed. by Jan-Christer Janson
title_full_unstemmed	Protein purification principles, high resolution methods, and applications ed. by Jan-Christer Janson
title_short	Protein purification
title_sort	protein purification principles high resolution methods and applications
title_sub	principles, high resolution methods, and applications
topic	Chromatographie (DE-588)4010153-8 gnd Trennverfahren (DE-588)4078395-9 gnd Elektrophorese (DE-588)4014373-9 gnd Reinigung (DE-588)4049250-3 gnd Proteine (DE-588)4076388-2 gnd
topic_facet	Chromatographie Trennverfahren Elektrophorese Reinigung Proteine Aufsatzsammlung
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