Modulation of cytokine profiles in HaCaT keratinocytes and human fibroblasts by lithium, propranolol, and chloroquine as known trigger factors for psoriasis:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Mikrofilm Buch |
| Sprache: | English |
| Veröffentlicht: |
2006
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Kiel, Univ., Diss., 2006 |
| Beschreibung: | 54 Bl. Ill., graph. Darst. |
Internformat
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| 245 | 1 | 0 | |a Modulation of cytokine profiles in HaCaT keratinocytes and human fibroblasts by lithium, propranolol, and chloroquine as known trigger factors for psoriasis |c vorgelegt von Jianping Cen |
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| 650 | 4 | |a Chloroquin | |
| 650 | 4 | |a Cytokine | |
| 650 | 4 | |a Fibroblast | |
| 650 | 4 | |a Hochschulschrift | |
| 650 | 4 | |a Keratinozyt | |
| 650 | 4 | |a Lithium | |
| 650 | 4 | |a Propranolol | |
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Datensatz im Suchindex
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|---|---|
| adam_text | 1. Introduction 1
1.1 Background 1
1.2 Purpose of the study 2
2. Materials and methods 4
2.1 Cell culture 4
2.1.1 Cell lines
2.1.2 Cell culture conditions 4
2.2. Treatment of cells 4
2.3. Detection of multiple cytokines in cell ly sate 5
2.4. Detection of multiple cytokines in cell culture supernatant 6
2.4.1 Interpretation of results 6
2.5. Reverse transcriptase (RT) PCR assays 6
2.5.1 Preparation of drugs 6
2.5.2 Treatment of cells with lithium carbonate and TNFa 6
2.5.3 Treatment of cells with propranolol hydrochloride and TNFa 7
2.5.4 RNA isolation 7
2.5.5 Reverse transcription 8
2.5.6 Real time PCR 8
2.5.7 Statistical analysis 10
3. Results 11
3.1. Expression of cytokines in cell lysates of human fibroblasts
treated with lithium carbonate, propranolol hydrochloride
and stimulated with TNFa 11
3.2. Expression of cytokines and growth factors in the culture
supematants of HaCaT cells treated with lithium carbonate,
propranolol hydrochloride, or chloroquine diphosphate
and stimulated with TNFa 12
3.3. Expression of cytokines and growth factors in the culture
supematants of fibroblasts treated with lithium carbonate or
propranolol hydrochloride and stimulated with TNFa 13
3.4. Comparison of cytokine expression in the culture
supematants of fibroblasts treated with TNFa alone
to fibroblasts cultured with medium alone 14
3.5. Expression of IL 8 mRNA in HaCaT cells after treatment
with lithium carbonate and subsequent stimulation with TNFa 15
3.6. Expression of IL 8 mRNA in HaCaT cells after treatment with
Propranolol hydrochloride and subsequent stimulation with TNFa 15
3.7. Expression of IL 8 mRNA in fibroblasts after treatment
with lithium carbonate and subsequent stimulation with TNFa 16
3.8. Expression of IL 8 mRNA in fibroblasts after treatment with
Propranolol hydrochloride and subsequent stimulation with TNFa 16
4. Discussion jg
5. Summary 28
6. Reference 30
7. Figures and legends 40
8. Acknowledgements 52
9. Curriculum vitae 53
|
| adam_txt |
1. Introduction 1
1.1 Background 1
1.2 Purpose of the study 2
2. Materials and methods 4
2.1 Cell culture 4
2.1.1 Cell lines
2.1.2 Cell culture conditions 4
2.2. Treatment of cells 4
2.3. Detection of multiple cytokines in cell ly sate 5
2.4. Detection of multiple cytokines in cell culture supernatant 6
2.4.1 Interpretation of results 6
2.5. Reverse transcriptase (RT) PCR assays 6
2.5.1 Preparation of drugs 6
2.5.2 Treatment of cells with lithium carbonate and TNFa 6
2.5.3 Treatment of cells with propranolol hydrochloride and TNFa 7
2.5.4 RNA isolation 7
2.5.5 Reverse transcription 8
2.5.6 Real time PCR 8
2.5.7 Statistical analysis 10
3. Results 11
3.1. Expression of cytokines in cell lysates of human fibroblasts
treated with lithium carbonate, propranolol hydrochloride
and stimulated with TNFa 11
3.2. Expression of cytokines and growth factors in the culture
supematants of HaCaT cells treated with lithium carbonate,
propranolol hydrochloride, or chloroquine diphosphate
and stimulated with TNFa 12
3.3. Expression of cytokines and growth factors in the culture
supematants of fibroblasts treated with lithium carbonate or
propranolol hydrochloride and stimulated with TNFa 13
3.4. Comparison of cytokine expression in the culture
supematants of fibroblasts treated with TNFa alone
to fibroblasts cultured with medium alone 14
3.5. Expression of IL 8 mRNA in HaCaT cells after treatment
with lithium carbonate and subsequent stimulation with TNFa 15
3.6. Expression of IL 8 mRNA in HaCaT cells after treatment with
Propranolol hydrochloride and subsequent stimulation with TNFa 15
3.7. Expression of IL 8 mRNA in fibroblasts after treatment
with lithium carbonate and subsequent stimulation with TNFa 16
3.8. Expression of IL 8 mRNA in fibroblasts after treatment with
Propranolol hydrochloride and subsequent stimulation with TNFa 16
4. Discussion jg
5. Summary 28
6. Reference 30
7. Figures and legends 40
8. Acknowledgements 52
9. Curriculum vitae 53 |
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| author | Cen, Jianping 1970- |
| author_GND | (DE-588)131627252 |
| author_facet | Cen, Jianping 1970- |
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| author_sort | Cen, Jianping 1970- |
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| building | Verbundindex |
| bvnumber | BV022219042 |
| ctrlnum | (OCoLC)255116101 (DE-599)BVBBV022219042 |
| dewey-full | 616.5 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 616 - Diseases |
| dewey-raw | 616.5 |
| dewey-search | 616.5 |
| dewey-sort | 3616.5 |
| dewey-tens | 610 - Medicine and health |
| discipline | Medizin |
| discipline_str_mv | Medizin |
| format | Microfilm Book |
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| illustrated | Illustrated |
| index_date | 2024-07-02T16:28:30Z |
| indexdate | 2024-07-09T20:52:38Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-015430284 |
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| physical | 54 Bl. Ill., graph. Darst. |
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| spelling | Cen, Jianping 1970- Verfasser (DE-588)131627252 aut Modulation of cytokine profiles in HaCaT keratinocytes and human fibroblasts by lithium, propranolol, and chloroquine as known trigger factors for psoriasis vorgelegt von Jianping Cen 2006 54 Bl. Ill., graph. Darst. txt rdacontent h rdamedia he rdacarrier Kiel, Univ., Diss., 2006 Mikroform-Ausgabe [2006 ?] 1 Mikrofiche : 24x s2006 [Mikrofiche-Ausg.] Chloroquin Cytokine Fibroblast Hochschulschrift Keratinozyt Lithium Propranolol Schuppenflechte (DE-588)4113937-9 Hochschulschrift gnd-content Reproduktion von Cen, Jianping, 1970- Modulation of cytokine profiles in HaCaT keratinocytes and human fibroblasts by lithium, propranolol, and chloroquine as known trigger factors for psoriasis 2006 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=015430284&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Cen, Jianping 1970- Modulation of cytokine profiles in HaCaT keratinocytes and human fibroblasts by lithium, propranolol, and chloroquine as known trigger factors for psoriasis Chloroquin Cytokine Fibroblast Hochschulschrift Keratinozyt Lithium Propranolol Schuppenflechte |
| subject_GND | (DE-588)4113937-9 |
| title | Modulation of cytokine profiles in HaCaT keratinocytes and human fibroblasts by lithium, propranolol, and chloroquine as known trigger factors for psoriasis |
| title_auth | Modulation of cytokine profiles in HaCaT keratinocytes and human fibroblasts by lithium, propranolol, and chloroquine as known trigger factors for psoriasis |
| title_exact_search | Modulation of cytokine profiles in HaCaT keratinocytes and human fibroblasts by lithium, propranolol, and chloroquine as known trigger factors for psoriasis |
| title_exact_search_txtP | Modulation of cytokine profiles in HaCaT keratinocytes and human fibroblasts by lithium, propranolol, and chloroquine as known trigger factors for psoriasis |
| title_full | Modulation of cytokine profiles in HaCaT keratinocytes and human fibroblasts by lithium, propranolol, and chloroquine as known trigger factors for psoriasis vorgelegt von Jianping Cen |
| title_fullStr | Modulation of cytokine profiles in HaCaT keratinocytes and human fibroblasts by lithium, propranolol, and chloroquine as known trigger factors for psoriasis vorgelegt von Jianping Cen |
| title_full_unstemmed | Modulation of cytokine profiles in HaCaT keratinocytes and human fibroblasts by lithium, propranolol, and chloroquine as known trigger factors for psoriasis vorgelegt von Jianping Cen |
| title_short | Modulation of cytokine profiles in HaCaT keratinocytes and human fibroblasts by lithium, propranolol, and chloroquine as known trigger factors for psoriasis |
| title_sort | modulation of cytokine profiles in hacat keratinocytes and human fibroblasts by lithium propranolol and chloroquine as known trigger factors for psoriasis |
| topic | Chloroquin Cytokine Fibroblast Hochschulschrift Keratinozyt Lithium Propranolol Schuppenflechte |
| topic_facet | Chloroquin Cytokine Fibroblast Hochschulschrift Keratinozyt Lithium Propranolol Schuppenflechte |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=015430284&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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