Epigenetic changes in pancreatic cancer-related gene expression:
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Mikrofilm Buch |
| Sprache: | English |
| Veröffentlicht: |
2005
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Kiel, Univ., Diss., 2005 |
| Beschreibung: | IV, 81 Bl. Ill., graph. Darst. |
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Datensatz im Suchindex
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TABLE OF CONTENTS
ABBREVIATIONS
1. INTRODUCTION 1
1.1 Epigenetics 1
1.1.1 The history of epigenetics 1
1.1.2 The relationship between cancer and epigenetics 3
1.1.3 The pancreatic cancer 4
1.2 Four pancreatic-cancer-related genes identified in microarray-based
expression studies 5
1.2.1 PTTG1 gene 6
1.2.2 ECT2 gene 6
1.2.3 OSF-2 gene 7
1.2.4 PLAG1 gene 7
1.3 The aims of this study 7
2. MATERIALS AND METHODS 8
2.1 Cell lines and tissues 8
2.1.1 Cell lines and cell culture 8
2.1.2 Normal and tumor pancreatic tissues 9
2.2 Genomic DNA isolation 9
2.2.1 Genomic DNA isolation from cells 9
2.2.2 Genomic DNA isolation from tissues 10
2.3 Restriction endonucleases digestion 11
2.3.1 Isoschizomeric restriction endonucleases Mspl/Hpall 11
2.3.2 Restriction endonuclease isoschizomers Mspl/Hpali digestions 12
2.4 PCR analysis 13
2.4.1 Primers for the area of ccggs located in the upstream region of
the 4 genes 13
2.4.2 PCR reaction 14
2.5 RNA Isolation 15
2.5.1 RNA isolation from cells 15
ii
2.5.2 RNA isolation from tissues 15
2.6 RT-PCR analysis 16
2.6.1 Synthesis of cDNA by reverse transcription 16
2.6.2 Amplification of specific cDNA fragments by PCR 17
3. RESULTS 19
3.1 PTTG1 gene 19
3.1.1 RT-PCR results of PTTG1 19
3.1.2 DNA methylation in the upstream region of the PTTG1 gene 22
3.1.3 The summary of DNA methylation and expression of the PTTG1
gene in cell lines and tissues 30
3.2 ECT2 gene 32
3.2.1 RT-PCR results of ECT2 32
3.2.2 DNA methylation in the upstream region of the ECT2 gene 34
3.2.3 The summary of DNA methylation and mRNA expression of the
ECT2 gene in cell lines and tissues 41
3.3 OSF-2 gene 43
3.3.1 RT-PCR results of OSF-2 43
3.3.2 DNA methylation in the upstream region of the OSF-2 gene 45
3.3.3 The summary of DNA methylation and mRNA expression of the OSF-2
gene in cell lines and tissues 50
3.4 PLAG1 gene 53
3.4.1 RT-PCR results of PLAG1 53
3.4.2 DNA methylation in the upstream region of the PLAGIgene 55
3.4.3 The summary of DNA methylation and mRNA expression of the
PLAG1 gene in cell lines and tissues 57
4. DISCUSSION 59
5. SUMMARY 67
6. REFERENCES 68
ACKNOWLEDGEMENTS 79
CURRICULUM VITAE 80
|
| adam_txt |
i
TABLE OF CONTENTS
ABBREVIATIONS
1. INTRODUCTION 1
1.1 Epigenetics 1
1.1.1 The history of epigenetics 1
1.1.2 The relationship between cancer and epigenetics 3
1.1.3 The pancreatic cancer 4
1.2 Four pancreatic-cancer-related genes identified in microarray-based
expression studies 5
1.2.1 PTTG1 gene 6
1.2.2 ECT2 gene 6
1.2.3 OSF-2 gene 7
1.2.4 PLAG1 gene 7
1.3 The aims of this study 7
2. MATERIALS AND METHODS 8
2.1 Cell lines and tissues 8
2.1.1 Cell lines and cell culture 8
2.1.2 Normal and tumor pancreatic tissues 9
2.2 Genomic DNA isolation 9
2.2.1 Genomic DNA isolation from cells 9
2.2.2 Genomic DNA isolation from tissues 10
2.3 Restriction endonucleases digestion 11
2.3.1 Isoschizomeric restriction endonucleases Mspl/Hpall 11
2.3.2 Restriction endonuclease isoschizomers Mspl/Hpali digestions 12
2.4 PCR analysis 13
2.4.1 Primers for the area of ccggs located in the upstream region of
the 4 genes 13
2.4.2 PCR reaction 14
2.5 RNA Isolation 15
2.5.1 RNA isolation from cells 15
ii
2.5.2 RNA isolation from tissues 15
2.6 RT-PCR analysis 16
2.6.1 Synthesis of cDNA by reverse transcription 16
2.6.2 Amplification of specific cDNA fragments by PCR 17
3. RESULTS 19
3.1 PTTG1 gene 19
3.1.1 RT-PCR results of PTTG1 19
3.1.2 DNA methylation in the upstream region of the PTTG1 gene 22
3.1.3 The summary of DNA methylation and expression of the PTTG1
gene in cell lines and tissues 30
3.2 ECT2 gene 32
3.2.1 RT-PCR results of ECT2 32
3.2.2 DNA methylation in the upstream region of the ECT2 gene 34
3.2.3 The summary of DNA methylation and mRNA expression of the
ECT2 gene in cell lines and tissues 41
3.3 OSF-2 gene 43
3.3.1 RT-PCR results of OSF-2 43
3.3.2 DNA methylation in the upstream region of the OSF-2 gene 45
3.3.3 The summary of DNA methylation and mRNA expression of the OSF-2
gene in cell lines and tissues 50
3.4 PLAG1 gene 53
3.4.1 RT-PCR results of PLAG1 53
3.4.2 DNA methylation in the upstream region of the PLAGIgene 55
3.4.3 The summary of DNA methylation and mRNA expression of the
PLAG1 gene in cell lines and tissues 57
4. DISCUSSION 59
5. SUMMARY 67
6. REFERENCES 68
ACKNOWLEDGEMENTS 79
CURRICULUM VITAE 80 |
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| spelling | Zhang, Mangli 1973- Verfasser (DE-588)131627112 aut Epigenetic changes in pancreatic cancer-related gene expression vorgelegt von Mangli Zhang 2005 IV, 81 Bl. Ill., graph. Darst. txt rdacontent h rdamedia he rdacarrier Kiel, Univ., Diss., 2005 Mikroform-Ausgabe [2006 ?] 1 Mikrofiche : 24x Mikrofiche-Ausg.: [Mikrofiche-Ausg.] Signatur des reproduzierten Kulturobjekts: L_x001C_2006 MF 328 s2006 Bauchspeicheldrüsenkrebs DNS Genexpression Hochschulschrift Methylierung (DE-588)4113937-9 Hochschulschrift gnd-content Reproduktion von Zhang, Mangli, 1973- Epigenetic changes in pancreatic cancer-related gene expression 2005 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=015428275&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Zhang, Mangli 1973- Epigenetic changes in pancreatic cancer-related gene expression Bauchspeicheldrüsenkrebs DNS Genexpression Hochschulschrift Methylierung |
| subject_GND | (DE-588)4113937-9 |
| title | Epigenetic changes in pancreatic cancer-related gene expression |
| title_auth | Epigenetic changes in pancreatic cancer-related gene expression |
| title_exact_search | Epigenetic changes in pancreatic cancer-related gene expression |
| title_exact_search_txtP | Epigenetic changes in pancreatic cancer-related gene expression |
| title_full | Epigenetic changes in pancreatic cancer-related gene expression vorgelegt von Mangli Zhang |
| title_fullStr | Epigenetic changes in pancreatic cancer-related gene expression vorgelegt von Mangli Zhang |
| title_full_unstemmed | Epigenetic changes in pancreatic cancer-related gene expression vorgelegt von Mangli Zhang |
| title_short | Epigenetic changes in pancreatic cancer-related gene expression |
| title_sort | epigenetic changes in pancreatic cancer related gene expression |
| topic | Bauchspeicheldrüsenkrebs DNS Genexpression Hochschulschrift Methylierung |
| topic_facet | Bauchspeicheldrüsenkrebs DNS Genexpression Hochschulschrift Methylierung |
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