Evaluation of laser assisted lentiviral transgenesis in bovine:
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Abschlussarbeit Buch |
| Sprache: | English German |
| Veröffentlicht: |
München
Verl. Dr. Hut
2006
|
| Ausgabe: | 1. Aufl. |
| Schriftenreihe: | Tiermedizin
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | 69 S. Ill., graph. Darst. 21 cm |
| ISBN: | 3899634039 |
Internformat
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Datensatz im Suchindex
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| adam_text | Table of contents
1 Introduction.......................................................................................................1
2 Literature............................................................................................................3
2.1 Transgenesis in bovine and other livestock species.....................................3
2.1.1 Definitions.............................................................................................3
2.1.1.1 Pronuclear microinjection (Ml).......................................................4
2.1.1.2 Cloning via nuclear transfer (NT)...................................................6
2.1.2 Application fields for transgenic livestock............................................10
2.1.2.1 Agriculture....................................................................................10
2.1.2.2 Biopharmaceuticals (gene pharming)...........................................10
2.1.2.3 Disease resistance.......................................................................11
2.1.2.4 Disease models...........................................................................12
2.1.3 Viral transgenesis................................................................................12
2.1.3.1 Viral transgenesis in bovine.........................................................12
2.1.3.2 Vector design...............................................................................13
2.1.3.3 RNA virus vectors........................................................................15
2.1.3.4 Retrovirus.....................................................................................15
2.1.3.5 Lentiviral vectors..........................................................................17
2.2 In vitro production (IVP) of bovine embryos...............................................20
2.2.1 History.................................................................................................20
2.2.2 Recovery of ovaries and oocyte collection..........................................20
2.2.3 Assessing oocyte quality.....................................................................21
2.2.4 Oocyte maturation...............................................................................22
2.2.5 In vitro fertilization (IVF)......................................................................24
2.2.6 In vitro culture (IVC)............................................................................25
2.2.7 Zona pellucida.....................................................................................26
2.2.7.1 Structure and function:.................................................................26
2.2.7.2 Drilling the ZP..............................................................................27
2.2.8 Cumulus cells......................................................................................27
2.2.8.1 In vivo...........................................................................................27
2.Z8.2 In vitro..........................................................................................27
2.3 Laser application........................................................................................29
2.3.1 Presumptions for the ideal laser......................................................„.. 29
2.3.2 Lasers emitting at the UV spectrum (10 - 380 nm).............................29
2.3.3 Lasers emitting at the infrared spectrum (780 nm - 50 um)................30
3 Material and Methods......................................................................................31
3.1 Virus production.........................................................................................31
3.2 In vitro production of bovine embryos.........................................................31
3.3 Subzonal virus injection..............................................................................32
3.4 Microdrilling and virus coincubation............................................................33
3.5 Effect of treatment and developmental stage.............................................36
3.6 Determination of polyspermy rate...............................................................37
3.7 Statistics.....................................................................................................37
4 Results.............................................................................................................38
4.1 Effects on embryonic development............................................................38
4.2 Effects on transgene expression................................................................38
4.3 Effect of microdrilling of oocyteson polyspermy.........................................40
5 Discussion.......................................................................................................43
6 Summary..........................................................................................................48
7 Zusammenfassung.........................................................___..........................49
8 Publications.....................................................................................................50
9 References.......................................................................................................51
10 Acknowledgement.......................................................................................68
|
| adam_txt |
Table of contents
1 Introduction.1
2 Literature.3
2.1 Transgenesis in bovine and other livestock species.3
2.1.1 Definitions.3
2.1.1.1 Pronuclear microinjection (Ml).4
2.1.1.2 Cloning via nuclear transfer (NT).6
2.1.2 Application fields for transgenic livestock.10
2.1.2.1 Agriculture.10
2.1.2.2 Biopharmaceuticals (gene pharming).10
2.1.2.3 Disease resistance.11
2.1.2.4 Disease models.12
2.1.3 Viral transgenesis.12
2.1.3.1 Viral transgenesis in bovine.12
2.1.3.2 Vector design.13
2.1.3.3 RNA virus vectors.15
2.1.3.4 Retrovirus.15
2.1.3.5 Lentiviral vectors.17
2.2 In vitro production (IVP) of bovine embryos.20
2.2.1 History.20
2.2.2 Recovery of ovaries and oocyte collection.20
2.2.3 Assessing oocyte quality.21
2.2.4 Oocyte maturation.22
2.2.5 In vitro fertilization (IVF).24
2.2.6 In vitro culture (IVC).25
2.2.7 Zona pellucida.26
2.2.7.1 Structure and function:.26
2.2.7.2 Drilling the ZP.27
2.2.8 Cumulus cells.27
2.2.8.1 In vivo.27
2.Z8.2 In vitro.27
2.3 Laser application.29
2.3.1 Presumptions for the ideal laser.„. 29
2.3.2 Lasers emitting at the UV spectrum (10 - 380 nm).29
2.3.3 Lasers emitting at the infrared spectrum (780 nm - 50 um).30
3 Material and Methods.31
3.1 Virus production.31
3.2 In vitro production of bovine embryos.31
3.3 Subzonal virus injection.32
3.4 Microdrilling and virus coincubation.33
3.5 Effect of treatment and developmental stage.36
3.6 Determination of polyspermy rate.37
3.7 Statistics.37
4 Results.38
4.1 Effects on embryonic development.38
4.2 Effects on transgene expression.38
4.3 Effect of microdrilling of oocyteson polyspermy.40
5 Discussion.43
6 Summary.48
7 Zusammenfassung._.49
8 Publications.50
9 References.51
10 Acknowledgement.68 |
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| author | Ewerling, Sonja 1974- |
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| author_role | aut |
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| author_variant | s e se |
| building | Verbundindex |
| bvnumber | BV022206589 |
| ctrlnum | (OCoLC)180713900 (DE-599)BVBBV022206589 |
| dewey-full | 636.0821 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 636 - Animal husbandry |
| dewey-raw | 636.0821 |
| dewey-search | 636.0821 |
| dewey-sort | 3636.0821 |
| dewey-tens | 630 - Agriculture and related technologies |
| discipline | Agrar-/Forst-/Ernährungs-/Haushaltswissenschaft / Gartenbau |
| discipline_str_mv | Agrar-/Forst-/Ernährungs-/Haushaltswissenschaft / Gartenbau |
| edition | 1. Aufl. |
| format | Thesis Book |
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| index_date | 2024-07-02T16:25:37Z |
| indexdate | 2024-07-09T20:52:22Z |
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| language | English German |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-015417969 |
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| spelling | Ewerling, Sonja 1974- Verfasser (DE-588)132348527 aut Evaluation of laser assisted lentiviral transgenesis in bovine by Sonja Ewerling 1. Aufl. München Verl. Dr. Hut 2006 69 S. Ill., graph. Darst. 21 cm txt rdacontent n rdamedia nc rdacarrier Tiermedizin Zugl.: München, Univ., Diss., 2006 Rind (DE-588)4050061-5 gnd rswk-swf Transgene Tiere (DE-588)4279021-9 gnd rswk-swf Virusvektor (DE-588)4232137-2 gnd rswk-swf Lentiviren (DE-588)4276396-4 gnd rswk-swf Lasertechnologie (DE-588)4166821-2 gnd rswk-swf Gentransfer (DE-588)4194102-0 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Rind (DE-588)4050061-5 s Transgene Tiere (DE-588)4279021-9 s Gentransfer (DE-588)4194102-0 s Lentiviren (DE-588)4276396-4 s Virusvektor (DE-588)4232137-2 s Lasertechnologie (DE-588)4166821-2 s DE-604 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=015417969&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Ewerling, Sonja 1974- Evaluation of laser assisted lentiviral transgenesis in bovine Rind (DE-588)4050061-5 gnd Transgene Tiere (DE-588)4279021-9 gnd Virusvektor (DE-588)4232137-2 gnd Lentiviren (DE-588)4276396-4 gnd Lasertechnologie (DE-588)4166821-2 gnd Gentransfer (DE-588)4194102-0 gnd |
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| title | Evaluation of laser assisted lentiviral transgenesis in bovine |
| title_auth | Evaluation of laser assisted lentiviral transgenesis in bovine |
| title_exact_search | Evaluation of laser assisted lentiviral transgenesis in bovine |
| title_exact_search_txtP | Evaluation of laser assisted lentiviral transgenesis in bovine |
| title_full | Evaluation of laser assisted lentiviral transgenesis in bovine by Sonja Ewerling |
| title_fullStr | Evaluation of laser assisted lentiviral transgenesis in bovine by Sonja Ewerling |
| title_full_unstemmed | Evaluation of laser assisted lentiviral transgenesis in bovine by Sonja Ewerling |
| title_short | Evaluation of laser assisted lentiviral transgenesis in bovine |
| title_sort | evaluation of laser assisted lentiviral transgenesis in bovine |
| topic | Rind (DE-588)4050061-5 gnd Transgene Tiere (DE-588)4279021-9 gnd Virusvektor (DE-588)4232137-2 gnd Lentiviren (DE-588)4276396-4 gnd Lasertechnologie (DE-588)4166821-2 gnd Gentransfer (DE-588)4194102-0 gnd |
| topic_facet | Rind Transgene Tiere Virusvektor Lentiviren Lasertechnologie Gentransfer Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=015417969&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT ewerlingsonja evaluationoflaserassistedlentiviraltransgenesisinbovine |