Biosynthetic potential of cyanobacteria: Isolation of bioactive peptides from the cyanobacterium Tychonema sp.
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | German |
| Veröffentlicht: |
München
Verl. Dr. Hut
2006
|
| Ausgabe: | 1. Aufl. |
| Schriftenreihe: | Pharmazeutische Biologie
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Zugl.: Bonn, Univ., Diss., 2006 |
| Beschreibung: | XII, 225 S. Ill., graph. Darst., Tab. 210 mm x 148 mm, 334 gr. |
| ISBN: | 9783899634075 3899634071 |
Internformat
MARC
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| 020 | |a 3899634071 |c Pb. : EUR 45.00 |9 3-89963-407-1 | ||
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| 084 | |a 610 |2 sdnb | ||
| 100 | 1 | |a Müller, Daniela |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Biosynthetic potential of cyanobacteria |b Isolation of bioactive peptides from the cyanobacterium Tychonema sp. |c Daniela Müller |
| 250 | |a 1. Aufl. | ||
| 264 | 1 | |a München |b Verl. Dr. Hut |c 2006 | |
| 300 | |a XII, 225 S. |b Ill., graph. Darst., Tab. |c 210 mm x 148 mm, 334 gr. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 0 | |a Pharmazeutische Biologie | |
| 500 | |a Zugl.: Bonn, Univ., Diss., 2006 | ||
| 650 | 0 | 7 | |a Bioaktive Verbindungen |0 (DE-588)4145589-7 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Tychonema |0 (DE-588)7642977-5 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Isolierung |g Chemie |0 (DE-588)4122223-4 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Peptide |0 (DE-588)4045125-2 |2 gnd |9 rswk-swf |
| 655 | 7 | |0 (DE-588)4113937-9 |a Hochschulschrift |2 gnd-content | |
| 689 | 0 | 0 | |a Tychonema |0 (DE-588)7642977-5 |D s |
| 689 | 0 | 1 | |a Peptide |0 (DE-588)4045125-2 |D s |
| 689 | 0 | 2 | |a Bioaktive Verbindungen |0 (DE-588)4145589-7 |D s |
| 689 | 0 | 3 | |a Isolierung |g Chemie |0 (DE-588)4122223-4 |D s |
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Datensatz im Suchindex
| _version_ | 1804135709483728896 |
|---|---|
| adam_text | TABLE
OF
CONTENTS
PAGE
1
INTRODUCTION
1
1.1
CYANOBACTERIA
-
AN
ANCIENT
GROUP
OF
PROKARYOTIC
ORGANISMS
1
1.2
BIOSYNTHETIC
POTENTIAL
OF
CYANOBACTERIA
3
1.2.1
NONRIBOSOMAL
PEPTIDE
SYNTHETASES
(NRPSS)
3
1.2.2
POLYKETIDE
SYNTHASES
(PKSS)
7
1.3
BIOLOGICAL
ACTIVITY
OF
CYANOBACTERIAL
SECONDARY
METABOLITES
WITH
FOCUS
ON
CYANOBACTERIAL
PEPTIDES
12
2
SCOPE
OF
THE
PRESENT
STUDY
28
3
MATERIAL
AND
METHODS
(GENERAL
PROCEDURES)
29
3.1
CYANOBACTERIAL
STRAINS
29
3.1.1
ORIGIN
OF
CYANOBACTERIAL
STRAINS
29
3.1.2
MAINTENANCE
OF
CYANOBACTERIAL
STOCK
CULTURES
33
3.1.3
CULTIVATION
OF
CYANOBACTERIA
FOR
SCREENING
PURPOSE
33
3.1.4
CULTIVATION
OF
TYCHONEMA
SP.
FOR
CHEMICAL
INVESTIGATIONS
34
3.2
EXTRACTION
OF
LYOPHILIZED
CYANOBACTERIAL
BIOMASS
3
5
3.2.1
CYANOBACTERIA
FOR
SCREENING
PURPOSE
35
3.2.2
EXTRACTION
OF
LARGE
SCALE
CULTURE
OF
TYCHONEMA
SP.
(PL
1)
35
3.3
MOLECULAR
BIOLOGICAL
METHODS
36
3.3.1
STERILIZATION
36
3.3.2
DNA
ISOLATION
36
3.3.2.1
ISOLATION
OF
CHROMOSOMAL
DNA
USING
QIAGEN S
DNEASY
TISSUE
KIT
36
3.3.2.2
ISOLATION
OF
CHROMOSOMAL
DNA
USING
NUCLEO
SPIN
TISSUE
KIT
(MACHEREY-NAGEL).
36
3.3.2.3
ISOLATION
OF
CHROMOSOMAL
DNA
ACCORDING
TO
SMOKER
ET
AL.
(1988)
37
3.3.2.4
ISOLATION
OF
CHROMOSOMAL
DNA
ACCORDING
TO
GIOVANNONI
ET
AL.
(1990)
38
3.3.3
DETERMINATION
OF
DNA
CONCENTRATION
AND
PURITY
3
8
3.3.4
RESTRICTION
ENZYME
DIGESTION
OF
DNA
FOR
SOUTHERN
ANALYSIS
38
3.3.5
AMPLIFICATION
OF
DNA
USING
POLYMERASE
CHAIN
REACTION
(PCR)
38
3.3.5.1
PCR
PROTOCOLS
39
II
TABLE
OF
CONTENTS
3.3.5.2
PCR
PROGRAMS
41
3.3.6
AGAROSE-GEL
ELECTROPHORESIS
43
3.3.7
DNA
EXTRACTION
FROM
AGAROSE
GELS
43
3.3.8
PREPARATION
OF
E.
COLI
COMPETENT
CELLS
(XL-1
BLUE)
44
3.3.9
CLONING
OF
PCR
PRODUCTS
IN
E.
COLI
XL-1
BLUE
CELLS
44
3.3.10
PREPARATION
OF
PLASMIDS
45
3.3.11
AUTOMATED
DNA
SEQUENZING
45
3.3.12
SOUTHERN
BLOT
AND
HYBRIDIZATION
47
3.3.12.1
CONSTRUCTION
OF
HYBRIDIZATION
PROBES
47
3.3.12.2
SOUTHERN
BLOTTING
47
3.3.12.3
HYBRIDIZATION
OF
PROBES
WITH
IMMOBILIZED
NUCLEIC
ACIDS
48
3.3.12.4
REMOVAL
OF
EXCESSIVE
AND
UNSPECIFICALLY
BOUND
PROBES
48
3.3.12.5
DETECTION
49
3.3.12.6
REPROBING
49
3.3.13
1
6S
RDNA
ANALYSIS
AND
MOLECULAR
CHARACTERIZATION
49
3.4
BIOLOGICAL
SCREENING
51
3.4.1
AGAR
DIFFUSION
ASSAYS
51
3.4.2
CYTOTOXICITY
52
3.4.2.1
TEST
ON
ACTIVITY
AGAINST
THE
BRINE
SHRIMP
ARTEMIA
SALINA
AND
THE
NEMATODE
CAENORRHABDITIS
ELEGANS
52
3.4.2.2
CYTOTOXICITY
TEST
AGAINST
HUMAN
CANCER
CELL
LINES
53
3.4.3
TYROSINE
KINASE
ASSAY
55
3.4.4
HIV-1
REVERSE
TRANSCRIPTASE
ASSAY
56
3.4.5
PROTEASE
INHIBITION
EXPERIMENTS
57
3.4.5.1
INVESTIGATION
OF
CYSTEINE
PROTEASE
PAPAIN
INHIBITION
57
3.4.5.2
ASSAY
FOR
THE
EVALUATION
OF
THE
ACTIVITY
AGAINST
THE
CYSTEINE
PROTEASES
CATHEPSIN
D
AND
L
57
3.4.5.3
INVESTIGATION
OF
ACTIVITY
AGAINST
HUMAN
CARBOXYPEPTIDASE
A1
AND
B
57
3.4.6
P-SECRETASE
INHIBITION
EXPERIMENTS
57
3.4.7
PROTEIN
PHOSPHATASE
ASSAYS
58
3.4.8
EXPERIMENTS
CONCERNING
THE
INTERACTION
WITH
P-GLYCOPROTEIN
58
3.4.9
HSC70
ATPASE
ASSAY
59
3.4.10
REPORTER
GENE
ASSAYS
59
TABLE
OF
CONTENTS
III
3.4.11
INVESTIGATION
OF
ANTIADHESIVE
AND
ANTIMIGRATORY
PROPERTIES
61
3.5
CHROMATOGRAPHY
61
3.5.1
THIN
LAYER
CHROMATOGRAPHY
(TLC)
61
3.5.2
VACUUM-LIQUID
CHROMATOGRAPHY
(VLC)
61
3.5.3
HIGH
PERFORMANCE
LIQUID
CHROMATOGRAPHY
(HPLC)
62
3.6
STRUCTURE
ELUCIDATION
62
3.6.1
NMR
SPECTROSCOPY
62
3.6.2
MASS
SPECTROMETRY
63
3.6.3
UV
MEASUREMENTS
64
3.6.4
IR
SPECTROSCOPY
64
3.6.5
OPTICAL
ROTATION
64
3.6.6
MOLECULAR
MODELLING
65
3.7
AMINO
ACID
ANALYSIS
BY
CHIRAL
GC
65
3.7.1
ACIDIC
HYDROLYSIS
AND
CHEMICAL
DERIVATISATION
65
3.7.2
HYDRAZINOLYSIS
66
3.7.3
DETERMINATION
OF
ABSOLUTE
CONFIGURATION
OF
LYSINE
67
3.7.4
OZONOLYSIS
67
3.8
CHEMICALS
AND
OTHER
MATERIALS
68
3.8.1
CHEMICALS
AND
SOLVENTS
68
3.8.2
ENZYMES
70
3.8.3
COMMERCIAL
KITS
70
3.8.4
PLASMIDS
71
3.8.5
ESCHERICHIA
COLI
STRAINS
(COMPETENT
CELLS)
72
3.8.5.1
CULTIVATION
OF
RECOMBINANT
ESCHERICHIA
COLI
STRAINS
72
3.8.5.2
MAINTENANCE
OF
STOCK
CULTURES
72
3.8.6
PRIMER
73
3.8.7
MOLECULAR
WEIGHT
MARKERS
74
3.8.8
MEDIA
74
3.8.9
BUFFERS
AND
SOLUTIONS
74
3.8.10
OTHER
MATERIAL
75
IV
TABLE
OF
CONTENTS
4
RESULTS
77
4.1
SCREENING
STUDIES
OF
CYANOBACTERIAL
STRAINS
77
4.1.1
MOLECULAR
BIOLOGICAL
ANALYSIS
OF
THE
BIOSYNTHETIC
POTENTIAL
OF
CYANOBACTERIAL
STRAINS
77
4.1.1.1
SCREENING
FOR
POLYKETIDE
SYNTHASE
(PKS)
GENE
FRAGMENTS
78
4.1.1.2
SCREENING
FOR
NONRIBOSOMAL
PEPTIDE
SYNTHETASE
(NRPS)
GENE
FRAGMENTS
84
4.1.2
SCREENING
STUDIES
OF
CYANOBACTERIAL
EXTRACTS
93
4.1.2.1
CHEMICAL
SCREENING
OF
CYANOBACTERIA
94
4.1.2.2
BIOLOGICAL
ACTIVITY
OF
CRUDE
CYANOBACTERIAL
EXTRACTS
102
4.1.3
APPRAISAL
OF
SCREENING
RESULTS
105
4.2
INVESTIGATION
OF
TYCHONEMA
SP.
(P1
1)
106
4.2.1
DEVELOPMENT
OF
AN
ISOLATION
METHOD
FOR
CYCLIC
PEPTIDES
109
4.2.2
STRUCTURE
ELUCIDATION
OF
BRUNSVICAMIDES
A,
B
AND
C
114
4.2.3
CHARACTERISATION
OF
PEPTIDES
DM
4.1
AND
DM
4.2
126
4.2.4
BIOLOGICAL
ACTIVITY
OF
ISOLATED
PEPTIDES
129
5
DISCUSSION
139
5.1
SCREENING
OF
CYANOBACTERIAL
STRAINS
139
5.2
PEPTIDES
FROM
TYCHONEMA
SP.
145
5.2.1
ISOLATION
OF
CYANOBACTERIAL
PEPTIDES
145
5.2.2
BRUNSVICAMIDES
145
5.2.2.1
STRUCTURAL
FEATURES
145
5.2.2.2
BIOACTIVITY
148
5.2.2.3
BIOSYNTHETIC
CONSIDERATIONS
150
5.2.2.4
ECOLOGICAL
ASPECTS
152
5.2.2.4.1
ECOLOGICAL
FUNCTION
OF
CYANOBACTERIAL
PEPTIDES
152
5.2.2.4.2
ASSOCIATIONS
BETWEEN
CYANOBACTERIA
AND
MACROORGANISMS
154
6
SUMMARY
163
7
REFERENCES
166
TABLE
OF
CONTENTS
V
8
APPENDIX
188
8.1
SPECTRAL
DATA
OF
THE
BRUNSVICAMIDES
188
8.2
SEQUENCES
OF
NRPS
AND
PKS
GENE
FRAGMENTS
ISOLATED
WITHIN
THIS
STUDY
216
8.2.1
SEQUENCES
OF
NRPS
GENE
FRAGMENTS
216
8.2.2
SEQUENCES
OF
PKS
GENE
FRAGMENTS
219
8.3
16S
RDNA
SEQUENCE
OF
TYCHONEMA
SP.
224
|
| adam_txt |
TABLE
OF
CONTENTS
PAGE
1
INTRODUCTION
1
1.1
CYANOBACTERIA
-
AN
ANCIENT
GROUP
OF
PROKARYOTIC
ORGANISMS
1
1.2
BIOSYNTHETIC
POTENTIAL
OF
CYANOBACTERIA
3
1.2.1
NONRIBOSOMAL
PEPTIDE
SYNTHETASES
(NRPSS)
3
1.2.2
POLYKETIDE
SYNTHASES
(PKSS)
7
1.3
BIOLOGICAL
ACTIVITY
OF
CYANOBACTERIAL
SECONDARY
METABOLITES
WITH
FOCUS
ON
CYANOBACTERIAL
PEPTIDES
12
2
SCOPE
OF
THE
PRESENT
STUDY
28
3
MATERIAL
AND
METHODS
(GENERAL
PROCEDURES)
29
3.1
CYANOBACTERIAL
STRAINS
29
3.1.1
ORIGIN
OF
CYANOBACTERIAL
STRAINS
29
3.1.2
MAINTENANCE
OF
CYANOBACTERIAL
STOCK
CULTURES
33
3.1.3
CULTIVATION
OF
CYANOBACTERIA
FOR
SCREENING
PURPOSE
33
3.1.4
CULTIVATION
OF
TYCHONEMA
SP.
FOR
CHEMICAL
INVESTIGATIONS
34
3.2
EXTRACTION
OF
LYOPHILIZED
CYANOBACTERIAL
BIOMASS
3
5
3.2.1
CYANOBACTERIA
FOR
SCREENING
PURPOSE
35
3.2.2
EXTRACTION
OF
LARGE
SCALE
CULTURE
OF
TYCHONEMA
SP.
(PL
1)
35
3.3
MOLECULAR
BIOLOGICAL
METHODS
36
3.3.1
STERILIZATION
36
3.3.2
DNA
ISOLATION
36
3.3.2.1
ISOLATION
OF
CHROMOSOMAL
DNA
USING
QIAGEN'S
DNEASY
TISSUE
KIT
36
3.3.2.2
ISOLATION
OF
CHROMOSOMAL
DNA
USING
NUCLEO
SPIN
TISSUE
KIT
(MACHEREY-NAGEL).
36
3.3.2.3
ISOLATION
OF
CHROMOSOMAL
DNA
ACCORDING
TO
SMOKER
ET
AL.
(1988)
37
3.3.2.4
ISOLATION
OF
CHROMOSOMAL
DNA
ACCORDING
TO
GIOVANNONI
ET
AL.
(1990)
38
3.3.3
DETERMINATION
OF
DNA
CONCENTRATION
AND
PURITY
3
8
3.3.4
RESTRICTION
ENZYME
DIGESTION
OF
DNA
FOR
SOUTHERN
ANALYSIS
38
3.3.5
AMPLIFICATION
OF
DNA
USING
POLYMERASE
CHAIN
REACTION
(PCR)
38
3.3.5.1
PCR
PROTOCOLS
39
II
TABLE
OF
CONTENTS
3.3.5.2
PCR
PROGRAMS
41
3.3.6
AGAROSE-GEL
ELECTROPHORESIS
43
3.3.7
DNA
EXTRACTION
FROM
AGAROSE
GELS
43
3.3.8
PREPARATION
OF
E.
COLI
COMPETENT
CELLS
(XL-1
BLUE)
44
3.3.9
CLONING
OF
PCR
PRODUCTS
IN
E.
COLI
XL-1
BLUE
CELLS
44
3.3.10
PREPARATION
OF
PLASMIDS
45
3.3.11
AUTOMATED
DNA
SEQUENZING
45
3.3.12
SOUTHERN
BLOT
AND
HYBRIDIZATION
47
3.3.12.1
CONSTRUCTION
OF
HYBRIDIZATION
PROBES
47
3.3.12.2
SOUTHERN
BLOTTING
47
3.3.12.3
HYBRIDIZATION
OF
PROBES
WITH
IMMOBILIZED
NUCLEIC
ACIDS
48
3.3.12.4
REMOVAL
OF
EXCESSIVE
AND
UNSPECIFICALLY
BOUND
PROBES
48
3.3.12.5
DETECTION
49
3.3.12.6
REPROBING
49
3.3.13
1
6S
RDNA
ANALYSIS
AND
MOLECULAR
CHARACTERIZATION
49
3.4
BIOLOGICAL
SCREENING
51
3.4.1
AGAR
DIFFUSION
ASSAYS
51
3.4.2
CYTOTOXICITY
52
3.4.2.1
TEST
ON
ACTIVITY
AGAINST
THE
BRINE
SHRIMP
ARTEMIA
SALINA
AND
THE
NEMATODE
CAENORRHABDITIS
ELEGANS
52
3.4.2.2
CYTOTOXICITY
TEST
AGAINST
HUMAN
CANCER
CELL
LINES
53
3.4.3
TYROSINE
KINASE
ASSAY
55
3.4.4
HIV-1
REVERSE
TRANSCRIPTASE
ASSAY
56
3.4.5
PROTEASE
INHIBITION
EXPERIMENTS
57
3.4.5.1
INVESTIGATION
OF
CYSTEINE
PROTEASE
PAPAIN
INHIBITION
57
3.4.5.2
ASSAY
FOR
THE
EVALUATION
OF
THE
ACTIVITY
AGAINST
THE
CYSTEINE
PROTEASES
CATHEPSIN
D
AND
L
57
3.4.5.3
INVESTIGATION
OF
ACTIVITY
AGAINST
HUMAN
CARBOXYPEPTIDASE
A1
AND
B
57
3.4.6
P-SECRETASE
INHIBITION
EXPERIMENTS
57
3.4.7
PROTEIN
PHOSPHATASE
ASSAYS
58
3.4.8
EXPERIMENTS
CONCERNING
THE
INTERACTION
WITH
P-GLYCOPROTEIN
58
3.4.9
HSC70
ATPASE
ASSAY
59
3.4.10
REPORTER
GENE
ASSAYS
59
TABLE
OF
CONTENTS
III
3.4.11
INVESTIGATION
OF
ANTIADHESIVE
AND
ANTIMIGRATORY
PROPERTIES
61
3.5
CHROMATOGRAPHY
61
3.5.1
THIN
LAYER
CHROMATOGRAPHY
(TLC)
61
3.5.2
VACUUM-LIQUID
CHROMATOGRAPHY
(VLC)
61
3.5.3
HIGH
PERFORMANCE
LIQUID
CHROMATOGRAPHY
(HPLC)
62
3.6
STRUCTURE
ELUCIDATION
62
3.6.1
NMR
SPECTROSCOPY
62
3.6.2
MASS
SPECTROMETRY
63
3.6.3
UV
MEASUREMENTS
64
3.6.4
IR
SPECTROSCOPY
64
3.6.5
OPTICAL
ROTATION
64
3.6.6
MOLECULAR
MODELLING
65
3.7
AMINO
ACID
ANALYSIS
BY
CHIRAL
GC
65
3.7.1
ACIDIC
HYDROLYSIS
AND
CHEMICAL
DERIVATISATION
65
3.7.2
HYDRAZINOLYSIS
66
3.7.3
DETERMINATION
OF
ABSOLUTE
CONFIGURATION
OF
LYSINE
67
3.7.4
OZONOLYSIS
67
3.8
CHEMICALS
AND
OTHER
MATERIALS
68
3.8.1
CHEMICALS
AND
SOLVENTS
68
3.8.2
ENZYMES
70
3.8.3
COMMERCIAL
KITS
70
3.8.4
PLASMIDS
71
3.8.5
ESCHERICHIA
COLI
STRAINS
(COMPETENT
CELLS)
72
3.8.5.1
CULTIVATION
OF
RECOMBINANT
ESCHERICHIA
COLI
STRAINS
72
3.8.5.2
MAINTENANCE
OF
STOCK
CULTURES
72
3.8.6
PRIMER
73
3.8.7
MOLECULAR
WEIGHT
MARKERS
74
3.8.8
MEDIA
74
3.8.9
BUFFERS
AND
SOLUTIONS
74
3.8.10
OTHER
MATERIAL
75
IV
TABLE
OF
CONTENTS
4
RESULTS
77
4.1
SCREENING
STUDIES
OF
CYANOBACTERIAL
STRAINS
77
4.1.1
MOLECULAR
BIOLOGICAL
ANALYSIS
OF
THE
BIOSYNTHETIC
POTENTIAL
OF
CYANOBACTERIAL
STRAINS
77
4.1.1.1
SCREENING
FOR
POLYKETIDE
SYNTHASE
(PKS)
GENE
FRAGMENTS
78
4.1.1.2
SCREENING
FOR
NONRIBOSOMAL
PEPTIDE
SYNTHETASE
(NRPS)
GENE
FRAGMENTS
84
4.1.2
SCREENING
STUDIES
OF
CYANOBACTERIAL
EXTRACTS
93
4.1.2.1
CHEMICAL
SCREENING
OF
CYANOBACTERIA
94
4.1.2.2
BIOLOGICAL
ACTIVITY
OF
CRUDE
CYANOBACTERIAL
EXTRACTS
102
4.1.3
APPRAISAL
OF
SCREENING
RESULTS
105
4.2
INVESTIGATION
OF
TYCHONEMA
SP.
(P1
1)
106
4.2.1
DEVELOPMENT
OF
AN
ISOLATION
METHOD
FOR
CYCLIC
PEPTIDES
109
4.2.2
STRUCTURE
ELUCIDATION
OF
BRUNSVICAMIDES
A,
B
AND
C
114
4.2.3
CHARACTERISATION
OF
PEPTIDES
DM
4.1
AND
DM
4.2
126
4.2.4
BIOLOGICAL
ACTIVITY
OF
ISOLATED
PEPTIDES
129
5
DISCUSSION
139
5.1
SCREENING
OF
CYANOBACTERIAL
STRAINS
139
5.2
PEPTIDES
FROM
TYCHONEMA
SP.
145
5.2.1
ISOLATION
OF
CYANOBACTERIAL
PEPTIDES
145
5.2.2
BRUNSVICAMIDES
145
5.2.2.1
STRUCTURAL
FEATURES
145
5.2.2.2
BIOACTIVITY
148
5.2.2.3
BIOSYNTHETIC
CONSIDERATIONS
150
5.2.2.4
ECOLOGICAL
ASPECTS
152
5.2.2.4.1
ECOLOGICAL
FUNCTION
OF
CYANOBACTERIAL
PEPTIDES
152
5.2.2.4.2
ASSOCIATIONS
BETWEEN
CYANOBACTERIA
AND
MACROORGANISMS
154
6
SUMMARY
163
7
REFERENCES
166
TABLE
OF
CONTENTS
V
8
APPENDIX
188
8.1
SPECTRAL
DATA
OF
THE
BRUNSVICAMIDES
188
8.2
SEQUENCES
OF
NRPS
AND
PKS
GENE
FRAGMENTS
ISOLATED
WITHIN
THIS
STUDY
216
8.2.1
SEQUENCES
OF
NRPS
GENE
FRAGMENTS
216
8.2.2
SEQUENCES
OF
PKS
GENE
FRAGMENTS
219
8.3
16S
RDNA
SEQUENCE
OF
TYCHONEMA
SP.
224 |
| any_adam_object | 1 |
| any_adam_object_boolean | 1 |
| author | Müller, Daniela |
| author_facet | Müller, Daniela |
| author_role | aut |
| author_sort | Müller, Daniela |
| author_variant | d m dm |
| building | Verbundindex |
| bvnumber | BV021796200 |
| ctrlnum | (OCoLC)162386532 (DE-599)BVBBV021796200 |
| dewey-full | 547.7 615.32939 |
| dewey-hundreds | 500 - Natural sciences and mathematics 600 - Technology (Applied sciences) |
| dewey-ones | 547 - Organic chemistry 615 - Pharmacology and therapeutics |
| dewey-raw | 547.7 615.32939 |
| dewey-search | 547.7 615.32939 |
| dewey-sort | 3547.7 |
| dewey-tens | 540 - Chemistry and allied sciences 610 - Medicine and health |
| discipline | Chemie / Pharmazie Medizin |
| discipline_str_mv | Chemie / Pharmazie Medizin |
| edition | 1. Aufl. |
| format | Book |
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| genre | (DE-588)4113937-9 Hochschulschrift gnd-content |
| genre_facet | Hochschulschrift |
| id | DE-604.BV021796200 |
| illustrated | Illustrated |
| index_date | 2024-07-02T15:46:13Z |
| indexdate | 2024-07-09T20:44:49Z |
| institution | BVB |
| isbn | 9783899634075 3899634071 |
| language | German |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-015008776 |
| oclc_num | 162386532 |
| open_access_boolean | |
| owner | DE-12 DE-19 DE-BY-UBM DE-29T |
| owner_facet | DE-12 DE-19 DE-BY-UBM DE-29T |
| physical | XII, 225 S. Ill., graph. Darst., Tab. 210 mm x 148 mm, 334 gr. |
| publishDate | 2006 |
| publishDateSearch | 2006 |
| publishDateSort | 2006 |
| publisher | Verl. Dr. Hut |
| record_format | marc |
| series2 | Pharmazeutische Biologie |
| spelling | Müller, Daniela Verfasser aut Biosynthetic potential of cyanobacteria Isolation of bioactive peptides from the cyanobacterium Tychonema sp. Daniela Müller 1. Aufl. München Verl. Dr. Hut 2006 XII, 225 S. Ill., graph. Darst., Tab. 210 mm x 148 mm, 334 gr. txt rdacontent n rdamedia nc rdacarrier Pharmazeutische Biologie Zugl.: Bonn, Univ., Diss., 2006 Bioaktive Verbindungen (DE-588)4145589-7 gnd rswk-swf Tychonema (DE-588)7642977-5 gnd rswk-swf Isolierung Chemie (DE-588)4122223-4 gnd rswk-swf Peptide (DE-588)4045125-2 gnd rswk-swf (DE-588)4113937-9 Hochschulschrift gnd-content Tychonema (DE-588)7642977-5 s Peptide (DE-588)4045125-2 s Bioaktive Verbindungen (DE-588)4145589-7 s Isolierung Chemie (DE-588)4122223-4 s DE-604 DNB Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=015008776&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Müller, Daniela Biosynthetic potential of cyanobacteria Isolation of bioactive peptides from the cyanobacterium Tychonema sp. Bioaktive Verbindungen (DE-588)4145589-7 gnd Tychonema (DE-588)7642977-5 gnd Isolierung Chemie (DE-588)4122223-4 gnd Peptide (DE-588)4045125-2 gnd |
| subject_GND | (DE-588)4145589-7 (DE-588)7642977-5 (DE-588)4122223-4 (DE-588)4045125-2 (DE-588)4113937-9 |
| title | Biosynthetic potential of cyanobacteria Isolation of bioactive peptides from the cyanobacterium Tychonema sp. |
| title_auth | Biosynthetic potential of cyanobacteria Isolation of bioactive peptides from the cyanobacterium Tychonema sp. |
| title_exact_search | Biosynthetic potential of cyanobacteria Isolation of bioactive peptides from the cyanobacterium Tychonema sp. |
| title_exact_search_txtP | Biosynthetic potential of cyanobacteria Isolation of bioactive peptides from the cyanobacterium Tychonema sp. |
| title_full | Biosynthetic potential of cyanobacteria Isolation of bioactive peptides from the cyanobacterium Tychonema sp. Daniela Müller |
| title_fullStr | Biosynthetic potential of cyanobacteria Isolation of bioactive peptides from the cyanobacterium Tychonema sp. Daniela Müller |
| title_full_unstemmed | Biosynthetic potential of cyanobacteria Isolation of bioactive peptides from the cyanobacterium Tychonema sp. Daniela Müller |
| title_short | Biosynthetic potential of cyanobacteria |
| title_sort | biosynthetic potential of cyanobacteria isolation of bioactive peptides from the cyanobacterium tychonema sp |
| title_sub | Isolation of bioactive peptides from the cyanobacterium Tychonema sp. |
| topic | Bioaktive Verbindungen (DE-588)4145589-7 gnd Tychonema (DE-588)7642977-5 gnd Isolierung Chemie (DE-588)4122223-4 gnd Peptide (DE-588)4045125-2 gnd |
| topic_facet | Bioaktive Verbindungen Tychonema Isolierung Chemie Peptide Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=015008776&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT mullerdaniela biosyntheticpotentialofcyanobacteriaisolationofbioactivepeptidesfromthecyanobacteriumtychonemasp |