Protein engineering protocols:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2007
|
| Schriftenreihe: | Methods in molecular biology
352 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Includes bibliographical references and index |
| Beschreibung: | XI, 312 S. Ill., graph. Darst. |
| ISBN: | 1588290727 9781588290724 |
Internformat
MARC
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| 245 | 1 | 0 | |a Protein engineering protocols |c ed. by Katja M. Arndt ... |
| 264 | 1 | |a Totowa, NJ |b Humana Press |c 2007 | |
| 300 | |a XI, 312 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
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| 490 | 1 | |a Methods in molecular biology |v 352 | |
| 500 | |a Includes bibliographical references and index | ||
| 650 | 4 | |a Technologie des protéines | |
| 650 | 4 | |a Protein engineering | |
| 650 | 0 | 7 | |a Biochemische Methode |0 (DE-588)4319880-6 |2 gnd |9 rswk-swf |
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Datensatz im Suchindex
| _version_ | 1804135537975492608 |
|---|---|
| adam_text | Contents
Preface v
Contributors ix
Part I Design and Computational Strategies for Protein Engineering
1 Combinatorial Protein Design Strategies
Using Computational Methods
Hidetoshi Kono, Wei Wang, and Jeffery G. Saven 3
2 Global Incorporation of Unnatural Amino Acids in Escherichia coli
Jamie M. Bacher and Andrew D. Ellington 23
3 Considerations in the Design and Optimization
of Coiled Coil Structures
Jody M. Mason, Kristian M. Miiller, and Katja M. Arndt 35
4 Calcium Indicators Based on Calmodulin Fluorescent Protein Fusions
Kevin Truong, Asako Sawano, Atsushi Miyawaki,
and Mitsuhiko Ikura 71
5 Design and Synthesis of Artificial Zinc Finger Proteins
Wataru Nomura and Yukio Sugiura 83
6 Monobodies: Antibody Mimics Based on the Scaffold
of the Fibronectin Type III Domain
Akiko Koide and Shohei Koide 95
7 Engineering Site Specific Endonucleases
Peter Friedhoff and Alfred Pingoud 111
Part II Evolutionary Strategies for Protein Engineering
8 Protein Library Design and Screening: Working Out the Probabilities
Michel Denault and Joelle N. Pelletier 127
9 Protein Design by Binary Patterning of Polar
and Nonpolar Amino Acids
Luke H. Bradley, Yinan Wei, Peter Thumfort, Christine Wurth,
and Michael H. Hecht 755
10 Versatile DNA Fragmentation and Directed Evolution
With Nucleotide Exchange and Excision Technology
Sabine C. Stebel, Katja M. Arndt, and Kristian M. Miiller 167
vii
viii Contents
11 Degenerate Oligonucleotide Gene Shuffling
Peter L. Bergquist and Moreland D. Gibbs 191
12 M1 3 Bacteriophage Coat Proteins Engineered
for Improved Phage Display
Sachdev S. Sidhu, Birte K. Feld, and Gregory A. Weiss 205
13 Ribosome lnactivation Display System
Satoshi Fujita, Jing Min Zhou, and Kazunari Taira 221
14 Compartmentalized Self Replication: A Novel Method
for the Directed Evolution of Polymerases and Other Enzymes
Farid ). Ghadessy and Philipp Holliger 237
1 5 Synthesis of Degenerated Libraries of the /?as Binding Domain
of Raf and Rapid Selection of Fast Folding and Stable Clones
With the Dihydrofolate Reductase Protein Fragment
Complementation Assay
Fran^ois Xavier Campbell Valois and Stephen W. Michnick 249
16 A General Method of Terminal Truncation, Evolution,
and Re Elongation to Generate Enzymes of Enhanced Stability
Jochen Hecky, Jody M. Mason, Katja M. Arndt,
and Kristian M. Muller 275
Index 305
|
| adam_txt |
Contents
Preface v
Contributors ix
Part I Design and Computational Strategies for Protein Engineering
1 Combinatorial Protein Design Strategies
Using Computational Methods
Hidetoshi Kono, Wei Wang, and Jeffery G. Saven 3
2 Global Incorporation of Unnatural Amino Acids in Escherichia coli
Jamie M. Bacher and Andrew D. Ellington 23
3 Considerations in the Design and Optimization
of Coiled Coil Structures
Jody M. Mason, Kristian M. Miiller, and Katja M. Arndt 35
4 Calcium Indicators Based on Calmodulin Fluorescent Protein Fusions
Kevin Truong, Asako Sawano, Atsushi Miyawaki,
and Mitsuhiko Ikura 71
5 Design and Synthesis of Artificial Zinc Finger Proteins
Wataru Nomura and Yukio Sugiura 83
6 Monobodies: Antibody Mimics Based on the Scaffold
of the Fibronectin Type III Domain
Akiko Koide and Shohei Koide 95
7 Engineering Site Specific Endonucleases
Peter Friedhoff and Alfred Pingoud 111
Part II Evolutionary Strategies for Protein Engineering
8 Protein Library Design and Screening: Working Out the Probabilities
Michel Denault and Joelle N. Pelletier 127
9 Protein Design by Binary Patterning of Polar
and Nonpolar Amino Acids
Luke H. Bradley, Yinan Wei, Peter Thumfort, Christine Wurth,
and Michael H. Hecht 755
10 Versatile DNA Fragmentation and Directed Evolution
With Nucleotide Exchange and Excision Technology
Sabine C. Stebel, Katja M. Arndt, and Kristian M. Miiller 167
vii
viii Contents
11 Degenerate Oligonucleotide Gene Shuffling
Peter L. Bergquist and Moreland D. Gibbs 191
12 M1 3 Bacteriophage Coat Proteins Engineered
for Improved Phage Display
Sachdev S. Sidhu, Birte K. Feld, and Gregory A. Weiss 205
13 Ribosome lnactivation Display System
Satoshi Fujita, Jing Min Zhou, and Kazunari Taira 221
14 Compartmentalized Self Replication: A Novel Method
for the Directed Evolution of Polymerases and Other Enzymes
Farid ). Ghadessy and Philipp Holliger 237
1 5 Synthesis of Degenerated Libraries of the /?as Binding Domain
of Raf and Rapid Selection of Fast Folding and Stable Clones
With the Dihydrofolate Reductase Protein Fragment
Complementation Assay
Fran^ois Xavier Campbell Valois and Stephen W. Michnick 249
16 A General Method of Terminal Truncation, Evolution,
and Re Elongation to Generate Enzymes of Enhanced Stability
Jochen Hecky, Jody M. Mason, Katja M. Arndt,
and Kristian M. Muller 275
Index 305 |
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| index_date | 2024-07-02T15:18:31Z |
| indexdate | 2024-07-09T20:42:05Z |
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| isbn | 1588290727 9781588290724 |
| language | English |
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| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spelling | Protein engineering protocols ed. by Katja M. Arndt ... Totowa, NJ Humana Press 2007 XI, 312 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 352 Includes bibliographical references and index Technologie des protéines Protein engineering Biochemische Methode (DE-588)4319880-6 gnd rswk-swf Proteindesign (DE-588)4346801-9 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Proteindesign (DE-588)4346801-9 s Biochemische Methode (DE-588)4319880-6 s b DE-604 Arndt, Katja M. Sonstige oth Methods in molecular biology 352 (DE-604)BV035362695 352 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014918819&sequence=000004&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Protein engineering protocols Methods in molecular biology Technologie des protéines Protein engineering Biochemische Methode (DE-588)4319880-6 gnd Proteindesign (DE-588)4346801-9 gnd |
| subject_GND | (DE-588)4319880-6 (DE-588)4346801-9 (DE-588)4143413-4 |
| title | Protein engineering protocols |
| title_auth | Protein engineering protocols |
| title_exact_search | Protein engineering protocols |
| title_exact_search_txtP | Protein engineering protocols |
| title_full | Protein engineering protocols ed. by Katja M. Arndt ... |
| title_fullStr | Protein engineering protocols ed. by Katja M. Arndt ... |
| title_full_unstemmed | Protein engineering protocols ed. by Katja M. Arndt ... |
| title_short | Protein engineering protocols |
| title_sort | protein engineering protocols |
| topic | Technologie des protéines Protein engineering Biochemische Methode (DE-588)4319880-6 gnd Proteindesign (DE-588)4346801-9 gnd |
| topic_facet | Technologie des protéines Protein engineering Biochemische Methode Proteindesign Aufsatzsammlung |
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