Cell-cell interactions: methods and protocols
Saved in:
| Format: | Book |
|---|---|
| Language: | English |
| Published: |
Totowa, NJ
Humana Press
2006
|
| Series: | Methods in molecular biology
341 |
| Subjects: | |
| Online Access: | Table of contents Inhaltsverzeichnis Klappentext |
| Item Description: | Includes bibliographical references and index |
| Physical Description: | XIII, 286 S. Ill., graph. Darst. |
| ISBN: | 1588295230 |
Staff View
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| 245 | 1 | 0 | |a Cell-cell interactions |b methods and protocols |c ed. by Sean P. Colgan |
| 264 | 1 | |a Totowa, NJ |b Humana Press |c 2006 | |
| 300 | |a XIII, 286 S. |b Ill., graph. Darst. | ||
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| 490 | 1 | |a Methods in molecular biology |v 341 | |
| 500 | |a Includes bibliographical references and index | ||
| 650 | 4 | |a Cell interaction | |
| 650 | 4 | |a Cell junctions | |
| 650 | 4 | |a Epithelial cells | |
| 650 | 4 | |a Cell Communication |x physiology | |
| 650 | 4 | |a Endothelial Cells |x physiology | |
| 650 | 4 | |a Epithelial Cells |x physiology | |
| 650 | 4 | |a Tight Junctions |x physiology | |
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Record in the Search Index
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| adam_text | CELL-CELL INTERACTIONS / COLGAN, SEAN P. : C2006
TABLE OF CONTENTS / INHALTSVERZEICHNIS
BLOOD/BRAIN BARRIER AND CELL/CELL INTERACTIONS: METHODS FOR ESTABLISHING IN VITRO MODELS OF THE BLOOD/BRAIN BARRIER AND TRANSPORT MEASUREMENTS / MICHAEL ASCHNER ... [ET AL.]
INTERACTIONS OF INTESTINAL EPITHELIAL CELLS WITH BACTERIA AND IMMUNE CELLS: METHODS TO CHARACTERIZE MICROFLORA AND FUNCTIONAL CONSEQUENCES / GERALDINE CANNY, ALEXANDER SWIDSINSKI, AND BETH A. MCCORMICK
THE ROLE OF JUNCTIONAL ADHESION MOLECULES IN INTERACTIONS BETWEEN VASCULAR CELLS / TRIANTAFYLLOS CHAVAKIS AND VALERIA ORLOVA
CELL/CELL INTERACTIONS ON SOLID MATRICES / NANCY A. LOUIS, DIONNE DANIELS, AND SEAN P. COLGAN
CELL/CELL INTERACTIONS IN THE KIDNEY: INDUCIBLE EXPRESSION OF MUTANT G PROTEIN -SUBUNITS IN MDCK CELLS FOR STUDIES OF EPITHELIAL CELL JUNCTION STRUCTURE AND FUNCTION / ERNESTO SABATH AND BRADLEY M. DENKER
NUCLEOTIDE METABOLISM AND CELL/CELL INTERACTIONS / HOLGER K. ELTZSCHIG ... [ET AL.]
ANALYSIS OF MAMMALIAN SPERM/EGG MEMBRANE INTERACTIONS DURING IN VITRO FERTILIZATION / GENEVIEVE B. WORTZMAN, ALLISON J. GARDNER, AND JANICE P. EVANS
COLLAGEN GEL CONTRACTION ASSAY / PETER NGO ... [ET AL.]
METHODS TO ASSESS TISSUE PERMEABILITY / JUAN C. IBLA AND JOSEPH K. KHOURY
A BIOCHEMICAL METHOD FOR TRACKING DOWN CHOLERA TOXIN TRANSPORT FROM PLASMA MEMBRANE TO GOLGI AND ENDOPLASMIC RETICULUM / HEIDI E. DELUCA AND WAYNE I. LENCER
ISOLATION AND CULTURE OF MURINE HEART AND LUNG ENDOTHELIAL CELLS FOR IN VITRO MODEL SYSTEMS / YAW-CHYN LIM AND FRANCES W. LUSCINSKAS
EXPRESSION CLONING OF SIGNALING PROTEINS REGULATED BY CELL ADHESION / MICHELLE L. MATTER AND JOE W. RAMOS
ASSAYS FOR THE CALCIUM SENSITIVITY OF DESMOSOMES / ANITA J. MERRITT, ANTHEA SCOTHERN, AND TANUSREE BHATTACHARYYA
TIGHT JUNCTIONS AND CELL/CELL INTERACTIONS / MARKUS UTECH, MATTHIAS BRUWER, AND ASMA NUSRAT
SIMULTANEOUS IMMUNOFLUORESCENCE DETECTION OF CADHERIN AND -CATENIN IN MOUSE TISSUE SECTIONS / NOBUKO OBARA
IN VITRO NEUTROPHIL TRANSEPITHELIAL MIGRATION / WINSTON Y. LEE ... [ET AL.]
THE BLOT RUNNING ASSAY: A METHOD FOR IDENTIFYING ADHESION MOLECULES MEDIATING BINDING UNDER SHEAR CONDITIONS / ROBERT SACKSTEIN AND ROBERT FUHLBRIGGE
CELL/CELL INTERACTION IN THE TRANSCELLULAR BIOSYNTHESIS OF NOVEL -3-DERIVED LIPID MEDIATORS / NAN CHIANG AND CHARLES N. SERHAN
ISOLATION AND ANALYSIS OF LIPID RAFTS IN CELL/CELL INTERACTIONS / AIMEE LANDRY AND RAMNIK XAVIER.
DIESES SCHRIFTSTUECK WURDE MASCHINELL ERZEUGT.
Cell-Cell Interactions
Methods and Protocols
Edited by
Sean P. Colgan
Center for Experimental Therapeutics and
Brigham and Women s Hospital, Harvard Medical School, Boston, MA
The use of directed cell models to study the manner in which two or more cells interact has
revolutionized our understanding of protein-protein interactions in a complex milieu and has provided an
invaluable platform for the identification of new molecules. In Cell-Cell Interactions: Methods and Proto¬
cols, highly skilled experts in the development and utilization of cell-cell interaction assays describe in
detail their best methods-based approaches, both in vitro and in vivo, for uncovering cellular interactions
at the molecular level. The protocols cover a diverse set of cell-cell interaction models in both normal and
pathological states, are readily adaptable to nearly any cell type and organ system, and include primary data
and outcome analysis. In addition, the protocols follow the successful Methods in Molecular Biology™
series format, each offering step-by-step laboratory instructions, an introduction outlining the principles
behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and
avoiding known pitfalls.
Insightful to beginners and inspiring to seasoned professionals, Cell-Cell Interactions: Methods and
Protocols provides the cutting-edge and versatile laboratory tools needed to gain a basic understanding of
cell-cell interactions.
FEATURES
Readily reproducible protocols on a diverse
set of celt-cell interaction models
Step-by-step cell-cell interaction assays
with primary data and outcome analysis
Methods that are readily adaptable for nearly any
cell type and organ system
Step-by-step instructions to ensure rapid
implementation and successful results
Tricks of the trade and notes on
troubleshooting and avoiding known pitfalls
CONTENTS
Blood-Brain Barrier and Cell-Cell Interactions: Methods for Es¬
tablishing In Vitro Models of the Blood-Brain Barrier and Trans¬
port Measurements. Interactions of Intestinal Epithelial Cells With
Bacteria and Immune Cells: Methods to Characterize
and Functional Consequences. The Role of Junctional Adhesion
Molecules in Interactions Between Vascular Cells. Cell-Cell In¬
teractions on Solid Matrices. Cell-Cell Interactions in the Kidney:
Inducible
Darby Canine Kidney Cells for Studies of Epithelial Cell ¡unction
Structure and Function. Nucleotide Metabolism and Cell-Cell
Interactions. Analysis of Mammalian Sperm-Egg Membrane In¬
teractions During In Vitro Fertilization. Collagen Gel Contraction
Assay. Methods to Assess Tissue Permeability. Bacterial-Bacteri¬
al
tercelular
Biochemical Method for Tracking Cholera Toxin Transport From
Plasma Membrane to Golgi and
Isolation and Culture of
for In Vitro Model Systems. Expression Cloning of Signaling Pro¬
teins Regulated by Cell Adhesion. Assays for the Calcium Sensi¬
tivity of Desmosomes. Tight Junctions and Cell-Cell Interactions.
Simultaneous Immunofluorescence Detection of Cadherin and
ß-Catenin in
itheliai Migration. The Blot Rolling Assay:
ing Adhesion Molecules Mediating Binding Under Shear Condi¬
tions. Cell-Cell Interaction in the Transcellular Biosynthesis of
Novel
Lipid
|
| adam_txt |
CELL-CELL INTERACTIONS / COLGAN, SEAN P. : C2006
TABLE OF CONTENTS / INHALTSVERZEICHNIS
BLOOD/BRAIN BARRIER AND CELL/CELL INTERACTIONS: METHODS FOR ESTABLISHING IN VITRO MODELS OF THE BLOOD/BRAIN BARRIER AND TRANSPORT MEASUREMENTS / MICHAEL ASCHNER . [ET AL.]
INTERACTIONS OF INTESTINAL EPITHELIAL CELLS WITH BACTERIA AND IMMUNE CELLS: METHODS TO CHARACTERIZE MICROFLORA AND FUNCTIONAL CONSEQUENCES / GERALDINE CANNY, ALEXANDER SWIDSINSKI, AND BETH A. MCCORMICK
THE ROLE OF JUNCTIONAL ADHESION MOLECULES IN INTERACTIONS BETWEEN VASCULAR CELLS / TRIANTAFYLLOS CHAVAKIS AND VALERIA ORLOVA
CELL/CELL INTERACTIONS ON SOLID MATRICES / NANCY A. LOUIS, DIONNE DANIELS, AND SEAN P. COLGAN
CELL/CELL INTERACTIONS IN THE KIDNEY: INDUCIBLE EXPRESSION OF MUTANT G PROTEIN -SUBUNITS IN MDCK CELLS FOR STUDIES OF EPITHELIAL CELL JUNCTION STRUCTURE AND FUNCTION / ERNESTO SABATH AND BRADLEY M. DENKER
NUCLEOTIDE METABOLISM AND CELL/CELL INTERACTIONS / HOLGER K. ELTZSCHIG . [ET AL.]
ANALYSIS OF MAMMALIAN SPERM/EGG MEMBRANE INTERACTIONS DURING IN VITRO FERTILIZATION / GENEVIEVE B. WORTZMAN, ALLISON J. GARDNER, AND JANICE P. EVANS
COLLAGEN GEL CONTRACTION ASSAY / PETER NGO . [ET AL.]
METHODS TO ASSESS TISSUE PERMEABILITY / JUAN C. IBLA AND JOSEPH K. KHOURY
A BIOCHEMICAL METHOD FOR TRACKING DOWN CHOLERA TOXIN TRANSPORT FROM PLASMA MEMBRANE TO GOLGI AND ENDOPLASMIC RETICULUM / HEIDI E. DELUCA AND WAYNE I. LENCER
ISOLATION AND CULTURE OF MURINE HEART AND LUNG ENDOTHELIAL CELLS FOR IN VITRO MODEL SYSTEMS / YAW-CHYN LIM AND FRANCES W. LUSCINSKAS
EXPRESSION CLONING OF SIGNALING PROTEINS REGULATED BY CELL ADHESION / MICHELLE L. MATTER AND JOE W. RAMOS
ASSAYS FOR THE CALCIUM SENSITIVITY OF DESMOSOMES / ANITA J. MERRITT, ANTHEA SCOTHERN, AND TANUSREE BHATTACHARYYA
TIGHT JUNCTIONS AND CELL/CELL INTERACTIONS / MARKUS UTECH, MATTHIAS BRUWER, AND ASMA NUSRAT
SIMULTANEOUS IMMUNOFLUORESCENCE DETECTION OF CADHERIN AND -CATENIN IN MOUSE TISSUE SECTIONS / NOBUKO OBARA
IN VITRO NEUTROPHIL TRANSEPITHELIAL MIGRATION / WINSTON Y. LEE . [ET AL.]
THE BLOT RUNNING ASSAY: A METHOD FOR IDENTIFYING ADHESION MOLECULES MEDIATING BINDING UNDER SHEAR CONDITIONS / ROBERT SACKSTEIN AND ROBERT FUHLBRIGGE
CELL/CELL INTERACTION IN THE TRANSCELLULAR BIOSYNTHESIS OF NOVEL -3-DERIVED LIPID MEDIATORS / NAN CHIANG AND CHARLES N. SERHAN
ISOLATION AND ANALYSIS OF LIPID RAFTS IN CELL/CELL INTERACTIONS / AIMEE LANDRY AND RAMNIK XAVIER.
DIESES SCHRIFTSTUECK WURDE MASCHINELL ERZEUGT.
Cell-Cell Interactions
Methods and Protocols
Edited by
Sean P. Colgan
Center for Experimental Therapeutics and
Brigham and Women's Hospital, Harvard Medical School, Boston, MA
The use of directed cell models to study the manner in which two or more cells interact has
revolutionized our understanding of protein-protein interactions in a complex milieu and has provided an
invaluable platform for the identification of new molecules. In Cell-Cell Interactions: Methods and Proto¬
cols, highly skilled experts in the development and utilization of cell-cell interaction assays describe in
detail their best methods-based approaches, both in vitro and in vivo, for uncovering cellular interactions
at the molecular level. The protocols cover a diverse set of cell-cell interaction models in both normal and
pathological states, are readily adaptable to nearly any cell type and organ system, and include primary data
and outcome analysis. In addition, the protocols follow the successful Methods in Molecular Biology™
series format, each offering step-by-step laboratory instructions, an introduction outlining the principles
behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and
avoiding known pitfalls.
Insightful to beginners and inspiring to seasoned professionals, Cell-Cell Interactions: Methods and
Protocols provides the cutting-edge and versatile laboratory tools needed to gain a basic understanding of
cell-cell interactions.
FEATURES
Readily reproducible protocols on a diverse
set of celt-cell interaction models
Step-by-step cell-cell interaction assays
with primary data and outcome analysis
Methods that are readily adaptable for nearly any
cell type and organ system
Step-by-step instructions to ensure rapid
implementation and successful results
Tricks of the trade and notes on
troubleshooting and avoiding known pitfalls
CONTENTS
Blood-Brain Barrier and Cell-Cell Interactions: Methods for Es¬
tablishing In Vitro Models of the Blood-Brain Barrier and Trans¬
port Measurements. Interactions of Intestinal Epithelial Cells With
Bacteria and Immune Cells: Methods to Characterize
and Functional Consequences. The Role of Junctional Adhesion
Molecules in Interactions Between Vascular Cells. Cell-Cell In¬
teractions on Solid Matrices. Cell-Cell Interactions in the Kidney:
Inducible
Darby Canine Kidney Cells for Studies of Epithelial Cell ¡unction
Structure and Function. Nucleotide Metabolism and Cell-Cell
Interactions. Analysis of Mammalian Sperm-Egg Membrane In¬
teractions During In Vitro Fertilization. Collagen Gel Contraction
Assay. Methods to Assess Tissue Permeability. Bacterial-Bacteri¬
al
tercelular
Biochemical Method for Tracking Cholera Toxin Transport From
Plasma Membrane to Golgi and
Isolation and Culture of
for In Vitro Model Systems. Expression Cloning of Signaling Pro¬
teins Regulated by Cell Adhesion. Assays for the Calcium Sensi¬
tivity of Desmosomes. Tight Junctions and Cell-Cell Interactions.
Simultaneous Immunofluorescence Detection of Cadherin and
ß-Catenin in
itheliai Migration. The Blot Rolling Assay:
ing Adhesion Molecules Mediating Binding Under Shear Condi¬
tions. Cell-Cell Interaction in the Transcellular Biosynthesis of
Novel
Lipid |
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| index_date | 2024-07-02T15:18:16Z |
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| isbn | 1588295230 |
| language | English |
| lccn | 2005029552 |
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| physical | XIII, 286 S. Ill., graph. Darst. |
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| series | Methods in molecular biology |
| series2 | Methods in molecular biology |
| spelling | Cell-cell interactions methods and protocols ed. by Sean P. Colgan Totowa, NJ Humana Press 2006 XIII, 286 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 341 Includes bibliographical references and index Cell interaction Cell junctions Epithelial cells Cell Communication physiology Endothelial Cells physiology Epithelial Cells physiology Tight Junctions physiology Methode (DE-588)4038971-6 gnd rswk-swf Zellkommunikation (DE-588)4136131-3 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Zellkommunikation (DE-588)4136131-3 s DE-604 Methode (DE-588)4038971-6 s b DE-604 Colgan, Sean P. Sonstige oth Methods in molecular biology 341 (DE-604)BV035362695 341 http://www.loc.gov/catdir/toc/ecip062/2005029552.html Table of contents LoC Fremddatenuebernahme application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014917866&sequence=000005&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014917866&sequence=000010&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA Klappentext |
| spellingShingle | Cell-cell interactions methods and protocols Methods in molecular biology Cell interaction Cell junctions Epithelial cells Cell Communication physiology Endothelial Cells physiology Epithelial Cells physiology Tight Junctions physiology Methode (DE-588)4038971-6 gnd Zellkommunikation (DE-588)4136131-3 gnd |
| subject_GND | (DE-588)4038971-6 (DE-588)4136131-3 (DE-588)4143413-4 |
| title | Cell-cell interactions methods and protocols |
| title_auth | Cell-cell interactions methods and protocols |
| title_exact_search | Cell-cell interactions methods and protocols |
| title_exact_search_txtP | Cell-cell interactions methods and protocols |
| title_full | Cell-cell interactions methods and protocols ed. by Sean P. Colgan |
| title_fullStr | Cell-cell interactions methods and protocols ed. by Sean P. Colgan |
| title_full_unstemmed | Cell-cell interactions methods and protocols ed. by Sean P. Colgan |
| title_short | Cell-cell interactions |
| title_sort | cell cell interactions methods and protocols |
| title_sub | methods and protocols |
| topic | Cell interaction Cell junctions Epithelial cells Cell Communication physiology Endothelial Cells physiology Epithelial Cells physiology Tight Junctions physiology Methode (DE-588)4038971-6 gnd Zellkommunikation (DE-588)4136131-3 gnd |
| topic_facet | Cell interaction Cell junctions Epithelial cells Cell Communication physiology Endothelial Cells physiology Epithelial Cells physiology Tight Junctions physiology Methode Zellkommunikation Aufsatzsammlung |
| url | http://www.loc.gov/catdir/toc/ecip062/2005029552.html http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014917866&sequence=000005&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014917866&sequence=000010&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV035362695 |
| work_keys_str_mv | AT colganseanp cellcellinteractionsmethodsandprotocols |