Ion channels: methods and protocols
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2006
|
| Schriftenreihe: | Methods in molecular biology
337 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis Klappentext |
| Beschreibung: | Includes bibliographical references and index |
| Beschreibung: | XII, 212 S. Ill., graph. Darst. |
| ISBN: | 1588295761 |
Internformat
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| 245 | 1 | 0 | |a Ion channels |b methods and protocols |c ed. by James D. Stockand ... |
| 264 | 1 | |a Totowa, NJ |b Humana Press |c 2006 | |
| 300 | |a XII, 212 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
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| 490 | 1 | |a Methods in molecular biology |v 337 | |
| 500 | |a Includes bibliographical references and index | ||
| 650 | 4 | |a Ion Channels | |
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Datensatz im Suchindex
| _version_ | 1804135501792280576 |
|---|---|
| adam_text | Contents
Preface
Contributors
Part I. Methods for Exogenous Expression of Ion Channels in Cells
1
in a Mammalian Expression System
Alexander Staruschenko, Rachell E. Booth, Oleh Pochynyuk,
fames D. Stockand, and Qiusheng
2
Replication-Deficient Virus
Richard M. Ahiquist and jane
3
the Biolistic Particle Delivery System
Nikita Gamper and Mark S. Shapiro
Part II. Methods for Studying Channel Structure-Function
4
Potassium Channels
John M.
5
of the Epithelial Sodium Channel Using the Xenopus laevis
Oocyte Expression System
Farhad
6
Energy Transfer Analysis
lie Zheng
Part III. Methods for Studying Channel Regulation and Physiological Function
7
Chou-Long Huang
ix
x
8
Bakhrom K. Berdiev and Dale J. Benos
9
Bound Ion Channel Density in Xenopus Oocytes
Mouhamed S. Away da, Weijian Shao,
and Abderrahmane Bengrine
10
for Electrophysiological Recording
Costa M. Colbert
11
of Extracellularly Recorded Neurons In Vivo
Glenn M. Toney and Lynette C. Daws
12
Channels and Secretion
Duk-SuKoh
Part IV. Methods for Studying Channelopathies, Genetic Screening,
and Molecular Biology
13
Mutants Using K+ Uptake-Deficient Escherichia
Lyubov V. Parfenova and Brad S.
14
Gildas
15
Andrée
Friedrich Beermann, and Edith Hummler.................................... 185
Index............................................................................................................207
Ion Channels
Methods and Protocols
Edited by
James D. Stockand and Mark S. Shapiro
University of Texas Health Science Center, San Antonio,
It is now clear that ion channels play essential roles in cell biology and physiology and that their
dysfunction is the root cause of many human diseases. In Ion Channels: Methods and Protocols, interna¬
tionally recognized
their use in successful experimental designs. The diverse applications range from the study of allosteric
regulation of ion channel activity using a classic mutagenesis approach, to the study of channel
stoichiometry using a novel biophysical approach based on fluorescence resonance energy transfer. High¬
lights include methods for heterologous expression of ¡on channels in cells, for determining channel
structure-function, for studying channel regulation and physiological function, and for genetic screening
and investigating channelopathies. The protocols follow the successful Methods in Molecular Biology™
series format, each offering step-by-step laboratory instructions, an introduction outlining the principles
behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and
avoiding known pitfalls.
Comprehensive and highly practical, Ion Channels: Methods and Protocols offer both novice and
experienced researchers a versatile collection of proven techniques for illuminating the structure, function,
and regulation of ion channel proteins and finding suitable drug targets when they cause disease.
FEATURES
Proven techniques for studying the structure,
function, and regulation of ion channels
Cutting-edge methods for genetic screening
and studying channelopathies
Readily reproducible techniques for heterologous
expression of ion channels in cells
Discussion of recent technological breakthroughs
and advanced experimental designs
Step-by-step instructions to ensure rapid implemen¬
tation and successful results
Tricks of the trade and notes on troubleshooting and
avoiding known pitfalls
CONTENTS
Part I. Methods for Exogenous Expression of Ion Channels in Cells.
Functional
Mammalian Expression System. Overexpression of Proteins in Neu¬
rons Using Replication-Deficient Virus. Exogenous Expression of
Proteins in Neurons Using the Biolistic Particle Delivery System. Part
II. Methods for Studying Channel Structure-Function. Tertiary and
Quaternary Structure Formation of Voltage-Gated Potassium Chan¬
nels. Biophysical Approach to Determine the
of the Epithelial Sodium Channel Using the Xenopus laevis Oocyte
Expression System. Spectroscopy-Based Quantitative Fluorescence
Resonance Energy Transfer Analysis. Part III. Methods for Studying
Channel Regulation and Physiological Function. Probing the Effects
of Phosphoinositides on Ion Channels. Epithelial Sodium Channel in
Planar
of Membrane-Bound Ion Channel Density in Xenopus Oocytes. Prep¬
aration of Cortical Brain Slices for Electrophysiological Recording,
luxtacellular Labeling and Chemical Phenotyping of Extracellularly
Recorded Neurons In Vivo. Carbon Fiber Amperometry in the Study
of Ion Channels and Secretion. Part IV. Methods for Studying Chan¬
nelopathies, Genetic Screening, and Molecular Biology. Genetic
Screening for Functionality of Bacterial Potassium Channel Mutants
Using K* Uptake-Deficient Eschericbia
Mediated Cardiac Channelopathies. Tissue-Specific Transgenic and
Knockout Mice. Index.
|
| adam_txt |
Contents
Preface
Contributors
Part I. Methods for Exogenous Expression of Ion Channels in Cells
1
in a Mammalian Expression System
Alexander Staruschenko, Rachell E. Booth, Oleh Pochynyuk,
fames D. Stockand, and Qiusheng
2
Replication-Deficient Virus
Richard M. Ahiquist and jane
3
the Biolistic Particle Delivery System
Nikita Gamper and Mark S. Shapiro
Part II. Methods for Studying Channel Structure-Function
4
Potassium Channels
John M.
5
of the Epithelial Sodium Channel Using the Xenopus laevis
Oocyte Expression System
Farhad
6
Energy Transfer Analysis
lie Zheng
Part III. Methods for Studying Channel Regulation and Physiological Function
7
Chou-Long Huang
ix
x
8
Bakhrom K. Berdiev and Dale J. Benos
9
Bound Ion Channel Density in Xenopus Oocytes
Mouhamed S. Away da, Weijian Shao,
and Abderrahmane Bengrine
10
for Electrophysiological Recording
Costa M. Colbert
11
of Extracellularly Recorded Neurons In Vivo
Glenn M. Toney and Lynette C. Daws
12
Channels and Secretion
Duk-SuKoh
Part IV. Methods for Studying Channelopathies, Genetic Screening,
and Molecular Biology
13
Mutants Using K+ Uptake-Deficient Escherichia
Lyubov V. Parfenova and Brad S.
14
Gildas
15
Andrée
Friedrich Beermann, and Edith Hummler. 185
Index.207
Ion Channels
Methods and Protocols
Edited by
James D. Stockand and Mark S. Shapiro
University of Texas Health Science Center, San Antonio,
It is now clear that ion channels play essential roles in cell biology and physiology and that their
dysfunction is the root cause of many human diseases. In Ion Channels: Methods and Protocols, interna¬
tionally recognized
their use in successful experimental designs. The diverse applications range from the study of allosteric
regulation of ion channel activity using a classic mutagenesis approach, to the study of channel
stoichiometry using a novel biophysical approach based on fluorescence resonance energy transfer. High¬
lights include methods for heterologous expression of ¡on channels in cells, for determining channel
structure-function, for studying channel regulation and physiological function, and for genetic screening
and investigating channelopathies. The protocols follow the successful Methods in Molecular Biology™
series format, each offering step-by-step laboratory instructions, an introduction outlining the principles
behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and
avoiding known pitfalls.
Comprehensive and highly practical, Ion Channels: Methods and Protocols offer both novice and
experienced researchers a versatile collection of proven techniques for illuminating the structure, function,
and regulation of ion channel proteins and finding suitable drug targets when they cause disease.
FEATURES
Proven techniques for studying the structure,
function, and regulation of ion channels
Cutting-edge methods for genetic screening
and studying channelopathies
Readily reproducible techniques for heterologous
expression of ion channels in cells
Discussion of recent technological breakthroughs
and advanced experimental designs
Step-by-step instructions to ensure rapid implemen¬
tation and successful results
Tricks of the trade and notes on troubleshooting and
avoiding known pitfalls
CONTENTS
Part I. Methods for Exogenous Expression of Ion Channels in Cells.
Functional
Mammalian Expression System. Overexpression of Proteins in Neu¬
rons Using Replication-Deficient Virus. Exogenous Expression of
Proteins in Neurons Using the Biolistic Particle Delivery System. Part
II. Methods for Studying Channel Structure-Function. Tertiary and
Quaternary Structure Formation of Voltage-Gated Potassium Chan¬
nels. Biophysical Approach to Determine the
of the Epithelial Sodium Channel Using the Xenopus laevis Oocyte
Expression System. Spectroscopy-Based Quantitative Fluorescence
Resonance Energy Transfer Analysis. Part III. Methods for Studying
Channel Regulation and Physiological Function. Probing the Effects
of Phosphoinositides on Ion Channels. Epithelial Sodium Channel in
Planar
of Membrane-Bound Ion Channel Density in Xenopus Oocytes. Prep¬
aration of Cortical Brain Slices for Electrophysiological Recording,
luxtacellular Labeling and Chemical Phenotyping of Extracellularly
Recorded Neurons In Vivo. Carbon Fiber Amperometry in the Study
of Ion Channels and Secretion. Part IV. Methods for Studying Chan¬
nelopathies, Genetic Screening, and Molecular Biology. Genetic
Screening for Functionality of Bacterial Potassium Channel Mutants
Using K* Uptake-Deficient Eschericbia
Mediated Cardiac Channelopathies. Tissue-Specific Transgenic and
Knockout Mice. Index. |
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| spelling | Ion channels methods and protocols ed. by James D. Stockand ... Totowa, NJ Humana Press 2006 XII, 212 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 337 Includes bibliographical references and index Ion Channels Ionenkanal (DE-588)4138699-1 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Ionenkanal (DE-588)4138699-1 s b DE-604 Stockand, James D. Sonstige oth Methods in molecular biology 337 (DE-604)BV035362695 337 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014895080&sequence=000007&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014895080&sequence=000008&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA Klappentext |
| spellingShingle | Ion channels methods and protocols Methods in molecular biology Ion Channels Ionenkanal (DE-588)4138699-1 gnd |
| subject_GND | (DE-588)4138699-1 (DE-588)4143413-4 |
| title | Ion channels methods and protocols |
| title_auth | Ion channels methods and protocols |
| title_exact_search | Ion channels methods and protocols |
| title_exact_search_txtP | Ion channels methods and protocols |
| title_full | Ion channels methods and protocols ed. by James D. Stockand ... |
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| title_short | Ion channels |
| title_sort | ion channels methods and protocols |
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