Integrated genomics: a discovery-based laboratory course
Gespeichert in:
| Hauptverfasser: | , , |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Chichester [u.a.]
Wiley
2006
|
| Schlagworte: | |
| Online-Zugang: | Table of contents Inhaltsverzeichnis |
| Beschreibung: | Includes bibliographical references and index |
| Beschreibung: | XX, 225 S. Ill. cm |
| ISBN: | 0470095016 9780470095010 0470095024 9780470095027 |
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| 100 | 1 | |a Caldwell, Guy A. |e Verfasser |4 aut | |
| 245 | 1 | 0 | |a Integrated genomics |b a discovery-based laboratory course |c Guy A. Caldwell, Shelli N. Williams and Kim A. Caldwell |
| 264 | 1 | |a Chichester [u.a.] |b Wiley |c 2006 | |
| 300 | |a XX, 225 S. |b Ill. |c cm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 500 | |a Includes bibliographical references and index | ||
| 650 | 4 | |a Genomics |v Laboratory manuals | |
| 650 | 4 | |a Diagnosis, Laboratory | |
| 650 | 4 | |a Molecular biology |x Technique | |
| 650 | 4 | |a Genomics |v Laboratory Manuals | |
| 650 | 4 | |a Genetic Techniques |v Laboratory Manuals | |
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| 700 | 1 | |a Williams, Shelli N. |e Verfasser |4 aut | |
| 700 | 1 | |a Caldwell, Kim A. |e Verfasser |4 aut | |
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Datensatz im Suchindex
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| adam_text | IMAGE 1
REFACE AUTHOR BIOGRAPHIES ACKNOWLEDGMENTS LIST OF FIGURES
1 INTRODUCTION TO BASIC LABORATORY GENETICS 1.1 TRANSFERRING AND
HANDLING C. ELEGANS 1.2 INTRODUCTION TO LABORATORY GENETICS
2 GENE EXPRESSION ANALYSIS USING TRANSGENIC ANIMALS 2.1 TRANSGENIC GENE
EXPRESSION ANALYSIS IN C. ELEGANS: L A D
STAINING
2.2 TRANSGENIC GENE EXPRESSION ANALYSIS IN C. ELEGANS: GFP ANALYSIS
XI
XIII XVII XIX
3 CREATION AND TESTING OF TRANSGENIC YEAST FOR USE IN PROTEIN - PROTEIN
INTERACTION SCREENING 27
3.1 SMALL-SCALE TRANSFORMATION OF S. CEREVISIAE 3 1
3.2 TRANSFORMATION OF S. CEREVISIAE TO TEST FOR NON-SPECIFIC INTERACTION
3 6 3.3 ASSAYING FOR PROTEIN - PROTEIN INTERACTION BY REPORTER GENE
EXPRESSION 42
4 YEAST TWO - HYBRID SCREENING 47
4.1 PROTEIN - PROTEIN INTERACTION SCREENING OF A C. ELEGANS CDNA LIBRARY
47 4.2 ASSAYING FOR PROTEIN - PROTEIN INTERACTION BY REPORTER GENE
EXPRESSION 54
IMAGE 2
VIII
CONTENTS
5
ISOLATION AND IDENTIFICATION OF INTERACTING PROTEINS 5.1 PREPARATION OF
ELECTROCOMPETENT E. COLI 5.2 ISOLATION OF DNA FROM YEAST AND
ELECTROPORATION OF E. 5-3
SMALL-SCALE ISOLATION OF PLASMID DNA FROM E. COLI: THE MINI-PREP 5.4
SEQUENCING OF TWO - HYBRID LIBRARY PLASMID DNA VECTORS
6 USING BIOINFORMATICS IN MODERN SCIENCE 6.1 DNA SEQUENCE CHROMATOGRAM
6.2 BLASTING YOUR SEQUENCE 6.3 EVALUATING SEQUENCE RESULTS AND CHOOSING
AN RNAI TARGET 6.4 BIOINFORMATICS PRACTICE QUESTIONS
7 GENERATION OF AN RNAI VECTOR SMALL - SCALE ISOLATION OF GENOMIC DNA
FROM C. ELEGANS PCR AMPLIFICATION OF TARGET GENE SEQUENCE FROM C.
ELEGANS GENOMIC DNA
PREPARATIONS FOR CLONING TO GENERATE RNAI VECTOR 7.3.1 AGAROSE GEL
ELECTROPHORESIS 7.3.2 REMOVAL OF DNTPS FROM PCR REACTION 7.3.3
RESTNCTION ENZYME DIGESTION OF PCR PRODUCT AND C. ELEGANS RNAI VECTOR
GEL PURIFICATION OF DNA AND LIGATION OF VECTOR AND PCR - AMPLIFIED DNA
7.4.1 PREPARATIVE AGAROSE GEL ELECTROPHORESIS 7.4.2 GEL PURIFICATION OF
DNA FROM AGAROSE GEL 7.4.3 LIGATION OF VECTOR AND PCR-AMPLIFIED DNA
TRANSFORMATION OF LIGATION REACTIONS PCR SCREENING OF TRANSFORMATION
COLONIES SMALL - SCALE ISOLATION OF PLASMID DNA FROM E. COLI: THE MINI -
PREP VERIFYING SUCCESSFUL LIGATION BY RESTRICTION DIGESTION
8 RNA - MEDIATED INTERFERENCE BY BACTERIAL FEEDING 8.1 PREPARATION OF
RNAI-FEEDING BACTERIA FOR TRANSFORMATION 8.2
MEDIA PREPARATION FOR RNAI FEEDING 8.3 TRANSFORMATION OF RNAI-FEEDING
STRAIN HT115(DE3) 8.4
RNA INTERFERENCE BY BACTERIAL FEEDING OF C. ELEGANS 8.5 ANALYZING
EFFECTS OF DSRNAI
8.5.1 ASSAYING FOR STEDITY (STE)
OR EMBRYONIC LETHALITY (EMB)
8.5.2
ASSAYING FOR GROWTH EFFECT 8.5.3
ASSAYING FOR MORPHOLOGICAL EFFECTS 8.5.4 ASSAYING FOR GENERAL
NEUROMUSCULAR EFFECTS 8.5.5 ASSAYING FOR SPECIFIC NEURONAL EFFECTS 8.5.6
ASSAYING FOR DAUER FORMATION
IMAGE 3
CONTENTS IX
APPENDIX I RECOMBINATIONAL CLONING AI. 1 ISOLATION OF
GENOMIC DNA FROM C. ELEGANS
A1.2 PCR AMPLIFICATION OF TARGET GENE SEQUENCE FRORN C. ELEGANS GENOMIC
DNA A1.3 AGAROSE GEL ELECTROPHORESIS AND CLEAN - UP OF PCR REACTION AI.4
ENTRY VECTOR CLONING
AI.5 SMALL - SCALE ISOLATION OF PLASMID DNA FROM E. COLI: THE MINI -
PREP AI.6 DESTINATION VECTOR CLONING A1.7 SMALL - SCALE ISOLATION OF
PLASMID DNA FROM E. COLI: THE MINI - PREP
APPENDIX I1
RECIPES AND MEDIA PREPARATION SOLUTION RECIPES MEDIA PREPARATION
APPENDIX I11
STERILE TECHNIQUES AND WORM PROTOCOLS 197 STERILE TECHNIQUES 197
WORM PROTOCOLS 199
APPENDIX IV MUTANT C. ELEGANS PHENOTYPES 205
APPENDIX V VECTOR MAPS 215
SUBJECT INDEX 219
|
| adam_txt |
IMAGE 1
REFACE AUTHOR BIOGRAPHIES ACKNOWLEDGMENTS LIST OF FIGURES
1 INTRODUCTION TO BASIC LABORATORY GENETICS 1.1 TRANSFERRING AND
HANDLING C. ELEGANS 1.2 INTRODUCTION TO LABORATORY GENETICS
2 GENE EXPRESSION ANALYSIS USING TRANSGENIC ANIMALS 2.1 TRANSGENIC GENE
EXPRESSION ANALYSIS IN C. ELEGANS: L A D
STAINING
2.2 TRANSGENIC GENE EXPRESSION ANALYSIS IN C. ELEGANS: GFP ANALYSIS
XI
XIII XVII XIX
3 CREATION AND TESTING OF TRANSGENIC YEAST FOR USE IN PROTEIN - PROTEIN
INTERACTION SCREENING 27
3.1 SMALL-SCALE TRANSFORMATION OF S. CEREVISIAE 3 1
3.2 TRANSFORMATION OF S. CEREVISIAE TO TEST FOR NON-SPECIFIC INTERACTION
3 6 3.3 ASSAYING FOR PROTEIN - PROTEIN INTERACTION BY REPORTER GENE
EXPRESSION 42
4 YEAST TWO - HYBRID SCREENING 47
4.1 PROTEIN - PROTEIN INTERACTION SCREENING OF A C. ELEGANS CDNA LIBRARY
47 4.2 ASSAYING FOR PROTEIN - PROTEIN INTERACTION BY REPORTER GENE
EXPRESSION 54
IMAGE 2
VIII
CONTENTS
5
ISOLATION AND IDENTIFICATION OF INTERACTING PROTEINS 5.1 PREPARATION OF
ELECTROCOMPETENT E. COLI 5.2 ISOLATION OF DNA FROM YEAST AND
ELECTROPORATION OF E. 5-3
SMALL-SCALE ISOLATION OF PLASMID DNA FROM E. COLI: THE MINI-PREP 5.4
SEQUENCING OF TWO - HYBRID LIBRARY PLASMID DNA VECTORS
6 USING BIOINFORMATICS IN MODERN SCIENCE 6.1 DNA SEQUENCE CHROMATOGRAM
6.2 BLASTING YOUR SEQUENCE 6.3 EVALUATING SEQUENCE RESULTS AND CHOOSING
AN RNAI TARGET 6.4 BIOINFORMATICS PRACTICE QUESTIONS
7 GENERATION OF AN RNAI VECTOR SMALL - SCALE ISOLATION OF GENOMIC DNA
FROM C. ELEGANS PCR AMPLIFICATION OF TARGET GENE SEQUENCE FROM C.
ELEGANS GENOMIC DNA
PREPARATIONS FOR CLONING TO GENERATE RNAI VECTOR 7.3.1 AGAROSE GEL
ELECTROPHORESIS 7.3.2 REMOVAL OF DNTPS FROM PCR REACTION 7.3.3
RESTNCTION ENZYME DIGESTION OF PCR PRODUCT AND C. ELEGANS RNAI VECTOR
GEL PURIFICATION OF DNA AND LIGATION OF VECTOR AND PCR - AMPLIFIED DNA
7.4.1 PREPARATIVE AGAROSE GEL ELECTROPHORESIS 7.4.2 GEL PURIFICATION OF
DNA FROM AGAROSE GEL 7.4.3 LIGATION OF VECTOR AND PCR-AMPLIFIED DNA
TRANSFORMATION OF LIGATION REACTIONS PCR SCREENING OF TRANSFORMATION
COLONIES SMALL - SCALE ISOLATION OF PLASMID DNA FROM E. COLI: THE MINI -
PREP VERIFYING SUCCESSFUL LIGATION BY RESTRICTION DIGESTION
8 RNA - MEDIATED INTERFERENCE BY BACTERIAL FEEDING 8.1 PREPARATION OF
RNAI-FEEDING BACTERIA FOR TRANSFORMATION 8.2
MEDIA PREPARATION FOR RNAI FEEDING 8.3 TRANSFORMATION OF RNAI-FEEDING
STRAIN HT115(DE3) 8.4
RNA INTERFERENCE BY BACTERIAL FEEDING OF C. ELEGANS 8.5 ANALYZING
EFFECTS OF DSRNAI
8.5.1 ASSAYING FOR STEDITY (STE)
OR EMBRYONIC LETHALITY (EMB)
8.5.2
ASSAYING FOR GROWTH EFFECT 8.5.3
ASSAYING FOR MORPHOLOGICAL EFFECTS 8.5.4 ASSAYING FOR GENERAL
NEUROMUSCULAR EFFECTS 8.5.5 ASSAYING FOR SPECIFIC NEURONAL EFFECTS 8.5.6
ASSAYING FOR DAUER FORMATION
IMAGE 3
CONTENTS IX
APPENDIX I RECOMBINATIONAL CLONING AI. 1 ISOLATION OF
GENOMIC DNA FROM C. ELEGANS
A1.2 PCR AMPLIFICATION OF TARGET GENE SEQUENCE FRORN C. ELEGANS GENOMIC
DNA A1.3 AGAROSE GEL ELECTROPHORESIS AND CLEAN - UP OF PCR REACTION AI.4
ENTRY VECTOR CLONING
AI.5 SMALL - SCALE ISOLATION OF PLASMID DNA FROM E. COLI: THE MINI -
PREP AI.6 DESTINATION VECTOR CLONING A1.7 SMALL - SCALE ISOLATION OF
PLASMID DNA FROM E. COLI: THE MINI - PREP
APPENDIX I1
RECIPES AND MEDIA PREPARATION SOLUTION RECIPES MEDIA PREPARATION
APPENDIX I11
STERILE TECHNIQUES AND WORM PROTOCOLS 197 STERILE TECHNIQUES 197
WORM PROTOCOLS 199
APPENDIX IV MUTANT C. ELEGANS PHENOTYPES 205
APPENDIX V VECTOR MAPS 215
SUBJECT INDEX 219 |
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| illustrated | Illustrated |
| index_date | 2024-07-02T15:06:29Z |
| indexdate | 2024-07-09T20:41:05Z |
| institution | BVB |
| isbn | 0470095016 9780470095010 0470095024 9780470095027 |
| language | English |
| lccn | 2006017997 |
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| publisher | Wiley |
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| spelling | Caldwell, Guy A. Verfasser aut Integrated genomics a discovery-based laboratory course Guy A. Caldwell, Shelli N. Williams and Kim A. Caldwell Chichester [u.a.] Wiley 2006 XX, 225 S. Ill. cm txt rdacontent n rdamedia nc rdacarrier Includes bibliographical references and index Genomics Laboratory manuals Diagnosis, Laboratory Molecular biology Technique Genomics Laboratory Manuals Genetic Techniques Laboratory Manuals Methode (DE-588)4038971-6 gnd rswk-swf Genomik (DE-588)4776397-8 gnd rswk-swf Gentechnologie (DE-588)4071722-7 gnd rswk-swf Genomik (DE-588)4776397-8 s Methode (DE-588)4038971-6 s DE-604 Gentechnologie (DE-588)4071722-7 s b DE-604 Williams, Shelli N. Verfasser aut Caldwell, Kim A. Verfasser aut http://www.loc.gov/catdir/toc/ecip0614/2006017997.html Table of contents OEBV Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014877097&sequence=000003&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Caldwell, Guy A. Williams, Shelli N. Caldwell, Kim A. Integrated genomics a discovery-based laboratory course Genomics Laboratory manuals Diagnosis, Laboratory Molecular biology Technique Genomics Laboratory Manuals Genetic Techniques Laboratory Manuals Methode (DE-588)4038971-6 gnd Genomik (DE-588)4776397-8 gnd Gentechnologie (DE-588)4071722-7 gnd |
| subject_GND | (DE-588)4038971-6 (DE-588)4776397-8 (DE-588)4071722-7 |
| title | Integrated genomics a discovery-based laboratory course |
| title_auth | Integrated genomics a discovery-based laboratory course |
| title_exact_search | Integrated genomics a discovery-based laboratory course |
| title_exact_search_txtP | Integrated genomics a discovery-based laboratory course |
| title_full | Integrated genomics a discovery-based laboratory course Guy A. Caldwell, Shelli N. Williams and Kim A. Caldwell |
| title_fullStr | Integrated genomics a discovery-based laboratory course Guy A. Caldwell, Shelli N. Williams and Kim A. Caldwell |
| title_full_unstemmed | Integrated genomics a discovery-based laboratory course Guy A. Caldwell, Shelli N. Williams and Kim A. Caldwell |
| title_short | Integrated genomics |
| title_sort | integrated genomics a discovery based laboratory course |
| title_sub | a discovery-based laboratory course |
| topic | Genomics Laboratory manuals Diagnosis, Laboratory Molecular biology Technique Genomics Laboratory Manuals Genetic Techniques Laboratory Manuals Methode (DE-588)4038971-6 gnd Genomik (DE-588)4776397-8 gnd Gentechnologie (DE-588)4071722-7 gnd |
| topic_facet | Genomics Laboratory manuals Diagnosis, Laboratory Molecular biology Technique Genomics Laboratory Manuals Genetic Techniques Laboratory Manuals Methode Genomik Gentechnologie |
| url | http://www.loc.gov/catdir/toc/ecip0614/2006017997.html http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014877097&sequence=000003&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT caldwellguya integratedgenomicsadiscoverybasedlaboratorycourse AT williamsshellin integratedgenomicsadiscoverybasedlaboratorycourse AT caldwellkima integratedgenomicsadiscoverybasedlaboratorycourse |