Calcium signaling protocols:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2006
|
| Ausgabe: | 2. ed. |
| Schriftenreihe: | Methods in molecular biology
312 |
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis Klappentext |
| Beschreibung: | X, 359 S. Ill., graph. Darst. |
| ISBN: | 1588294420 |
Internformat
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| 245 | 1 | 0 | |a Calcium signaling protocols |c ed. by David G. Lambert |
| 250 | |a 2. ed. | ||
| 264 | 1 | |a Totowa, NJ |b Humana Press |c 2006 | |
| 300 | |a X, 359 S. |b Ill., graph. Darst. | ||
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| 650 | 4 | |a Calcium Signaling |x physiology | |
| 650 | 4 | |a Calcium channels |x Research |x Methodology | |
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| 650 | 4 | |a Calcium |x Research |x Methodology | |
| 650 | 4 | |a Calcium |x pharmacokinetics | |
| 650 | 4 | |a Cellular signal transduction |x Research |x Methodology | |
| 650 | 4 | |a Signal Transduction | |
| 650 | 4 | |a Spectrometry, Fluorescence |x methods | |
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Datensatz im Suchindex
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|---|---|
| adam_text | Contents
Preface
Contributors
Part I. General
1
Alec W. M. Simpson
2
Robert A. Hirst, Charlotte Harrison, Kazuyoshi Hirota,
and David G. Lambert
3
Adriaan
Part II. Specialist Measurement Systems
4
Stephen C. Tovey,Paul). Brighton, and Gary B.
5
Ca2+ Concentration
Anthony J. Morgan and Andrew P. Thomas
6
Fluorometer
Ian C. B. Marshall, Izzy
7
the Fluorometric Imaging Plate Reader
Ian C. B. Marshall,
Part III. Measurement of Ca2+ Channel Activity
8
Mercedes Villarroya,
and Antonio
9
MVIIA Binding
Kazuyoshi Hirota and David G. Lambert
10
Channel Currents: Heterologous Expression Systems
and Dissociated Brain Neurons
Atticus H. Hainsworth, Andrew D. Randall,
and
vii
viii Contents
Part IV. Measurement of Ins(1,4,5)P3 and Ca2+ Release
From Intracellular Stores
11
Using [3H]lnositol-Labeling Protocols in Permeabiiized Cells
Alison Skippen,
12
Radioreceptor
Darren Smart
13
Using a 45Ca2+ Uptake and Release Assay
Robert A. Wilcox
14
Calcium-Mobilizing Agents Into Intact Adherent Cells
Robert A. Wilcox, Ian D. Forsythe, and Terence J. McCann
15
Stores Using Compartmentalized Fluorescent Indicators
Aldebaran M.
Part V. Specialist Measurement Techniques
16
Front-Surface Fluorimetry
Hideo Kanaide
17
Leong L. Ng and Paulene A. Quinn
18
Populations
Raul Martinez-Zaguilan, Linda S. Tompkins, Robert ¡. Gillies,
and Ronald M. Lynch
19
Martin R. McAinsh and
Part VI. Ch^-Sensitive Targets
20
Rajendra K. Sharma
21
Phosphodiesterase and Isozyme Separation
Rajendra K. Sharma
22
Danuta Kosk-Kosicka........................................................................343
index
Now extensively updated and expanded, Calcium Signaling Protocols, Second Edition
continues the excellence of the highly regarded first edition. New to this edition are chapters on
the electrophysiological measurement of Ca2+ channel activity, enhanced coverage of confocal
microscopy, and practical tutorials on two of the most common industrial high-throughput
machines, the Fluorometric Imaging Plate Reader (FLIPR), and the FlexStation. All experimental
chapters provide readily reproducible techniques for measuring the many facets of Ca2+signaling.
The protocols follow the successful Methods in Molecular Biology7 series format, each offering
step-by-step laboratory instructions, an introduction outlining the principles behind the tech¬
nique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding
known pitfalls.
Authoritative and up-to-date, Calcium Signaling Protocols, Second Edition provides for
novice and expert researchers alike both theoretical and practical information for the optimal
design and performance of studies of intracellular Ca2+regulation.
Up-to-date and readily reproducible tech-
niques
Proven techniques for measuring Ca2t channel
activity results
Fully tested methods for assessing the release
of stored Ca2+ ing and avoiding known pitfalls
|
| adam_txt |
Contents
Preface
Contributors
Part I. General
1
Alec W. M. Simpson
2
Robert A. Hirst, Charlotte Harrison, Kazuyoshi Hirota,
and David G. Lambert
3
Adriaan
Part II. Specialist Measurement Systems
4
Stephen C. Tovey,Paul). Brighton, and Gary B.
5
Ca2+ Concentration
Anthony J. Morgan and Andrew P. Thomas
6
Fluorometer
Ian C. B. Marshall, Izzy
7
the Fluorometric Imaging Plate Reader
Ian C. B. Marshall,
Part III. Measurement of Ca2+ Channel Activity
8
Mercedes Villarroya,
and Antonio
9
MVIIA Binding
Kazuyoshi Hirota and David G. Lambert
10
Channel Currents: Heterologous Expression Systems
and Dissociated Brain Neurons
Atticus H. Hainsworth, Andrew D. Randall,
and
vii
viii Contents
Part IV. Measurement of Ins(1,4,5)P3 and Ca2+ Release
From Intracellular Stores
11
Using [3H]lnositol-Labeling Protocols in Permeabiiized Cells
Alison Skippen,
12
Radioreceptor
Darren Smart
13
Using a 45Ca2+ Uptake and Release Assay
Robert A. Wilcox
14
Calcium-Mobilizing Agents Into Intact Adherent Cells
Robert A. Wilcox, Ian D. Forsythe, and Terence J. McCann
15
Stores Using Compartmentalized Fluorescent Indicators
Aldebaran M.
Part V. Specialist Measurement Techniques
16
Front-Surface Fluorimetry
Hideo Kanaide
17
Leong L. Ng and Paulene A. Quinn
18
Populations
Raul Martinez-Zaguilan, Linda S. Tompkins, Robert ¡. Gillies,
and Ronald M. Lynch
19
Martin R. McAinsh and
Part VI. Ch^-Sensitive Targets
20
Rajendra K. Sharma
21
Phosphodiesterase and Isozyme Separation
Rajendra K. Sharma
22
Danuta Kosk-Kosicka.343
index
Now extensively updated and expanded, Calcium Signaling Protocols, Second Edition
continues the excellence of the highly regarded first edition. New to this edition are chapters on
the electrophysiological measurement of Ca2+ channel activity, enhanced coverage of confocal
microscopy, and practical tutorials on two of the most common industrial high-throughput
machines, the Fluorometric Imaging Plate Reader (FLIPR), and the FlexStation. All experimental
chapters provide readily reproducible techniques for measuring the many facets of Ca2+signaling.
The protocols follow the successful Methods in Molecular Biology7" series format, each offering
step-by-step laboratory instructions, an introduction outlining the principles behind the tech¬
nique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding
known pitfalls.
Authoritative and up-to-date, Calcium Signaling Protocols, Second Edition provides for
novice and expert researchers alike both theoretical and practical information for the optimal
design and performance of studies of intracellular Ca2+regulation.
Up-to-date and readily reproducible tech-
niques
Proven techniques for measuring Ca2t channel
activity results
Fully tested methods for assessing the release
of stored Ca2+ ing and avoiding known pitfalls |
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| index_date | 2024-07-02T15:03:22Z |
| indexdate | 2024-07-09T20:40:50Z |
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| spelling | Calcium signaling protocols ed. by David G. Lambert 2. ed. Totowa, NJ Humana Press 2006 X, 359 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 312 Calcium - Manuels de laboratoire Calcium - Recherche - Méthodologie Calcium gtt Canaux à calcium - Recherche - Méthodologie Celbiologie gtt Transduction du signal cellulaire - Recherche - Méthodologie Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Calcium Channels pharmacokinetics Calcium Signaling physiology Calcium channels Research Methodology Calcium Laboratory manuals Calcium Research Methodology Calcium pharmacokinetics Cellular signal transduction Research Methodology Signal Transduction Spectrometry, Fluorescence methods Calciumkanal (DE-588)4205050-9 gnd rswk-swf Messung (DE-588)4038852-9 gnd rswk-swf Signaltransduktion (DE-588)4318717-1 gnd rswk-swf Calcium (DE-588)4069806-3 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Calcium (DE-588)4069806-3 s Signaltransduktion (DE-588)4318717-1 s DE-604 Calciumkanal (DE-588)4205050-9 s Messung (DE-588)4038852-9 s Lambert, David G. Sonstige oth Methods in molecular biology 312 (DE-604)BV035362695 312 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014866784&sequence=000003&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014866784&sequence=000004&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA Klappentext |
| spellingShingle | Calcium signaling protocols Methods in molecular biology Calcium - Manuels de laboratoire Calcium - Recherche - Méthodologie Calcium gtt Canaux à calcium - Recherche - Méthodologie Celbiologie gtt Transduction du signal cellulaire - Recherche - Méthodologie Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Calcium Channels pharmacokinetics Calcium Signaling physiology Calcium channels Research Methodology Calcium Laboratory manuals Calcium Research Methodology Calcium pharmacokinetics Cellular signal transduction Research Methodology Signal Transduction Spectrometry, Fluorescence methods Calciumkanal (DE-588)4205050-9 gnd Messung (DE-588)4038852-9 gnd Signaltransduktion (DE-588)4318717-1 gnd Calcium (DE-588)4069806-3 gnd |
| subject_GND | (DE-588)4205050-9 (DE-588)4038852-9 (DE-588)4318717-1 (DE-588)4069806-3 (DE-588)4143413-4 |
| title | Calcium signaling protocols |
| title_auth | Calcium signaling protocols |
| title_exact_search | Calcium signaling protocols |
| title_exact_search_txtP | Calcium signaling protocols |
| title_full | Calcium signaling protocols ed. by David G. Lambert |
| title_fullStr | Calcium signaling protocols ed. by David G. Lambert |
| title_full_unstemmed | Calcium signaling protocols ed. by David G. Lambert |
| title_short | Calcium signaling protocols |
| title_sort | calcium signaling protocols |
| topic | Calcium - Manuels de laboratoire Calcium - Recherche - Méthodologie Calcium gtt Canaux à calcium - Recherche - Méthodologie Celbiologie gtt Transduction du signal cellulaire - Recherche - Méthodologie Wetenschappelijke technieken gtt Wissenschaftliches Arbeiten Calcium Channels pharmacokinetics Calcium Signaling physiology Calcium channels Research Methodology Calcium Laboratory manuals Calcium Research Methodology Calcium pharmacokinetics Cellular signal transduction Research Methodology Signal Transduction Spectrometry, Fluorescence methods Calciumkanal (DE-588)4205050-9 gnd Messung (DE-588)4038852-9 gnd Signaltransduktion (DE-588)4318717-1 gnd Calcium (DE-588)4069806-3 gnd |
| topic_facet | Calcium - Manuels de laboratoire Calcium - Recherche - Méthodologie Calcium Canaux à calcium - Recherche - Méthodologie Celbiologie Transduction du signal cellulaire - Recherche - Méthodologie Wetenschappelijke technieken Wissenschaftliches Arbeiten Calcium Channels pharmacokinetics Calcium Signaling physiology Calcium channels Research Methodology Calcium Laboratory manuals Calcium Research Methodology Calcium pharmacokinetics Cellular signal transduction Research Methodology Signal Transduction Spectrometry, Fluorescence methods Calciumkanal Messung Signaltransduktion Aufsatzsammlung |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014866784&sequence=000003&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014866784&sequence=000004&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA |
| volume_link | (DE-604)BV035362695 |
| work_keys_str_mv | AT lambertdavidg calciumsignalingprotocols |