Plant proteomics: methods and protocols
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Totowa, NJ
Humana Press
2007
|
| Schriftenreihe: | Methods in molecular biology
355 |
| Schlagworte: | |
| Online-Zugang: | Klappentext Inhaltsverzeichnis |
| Beschreibung: | Includes bibliographical references and index |
| Beschreibung: | XIII, 399 S. Ill., graph. Darst. |
| ISBN: | 1588296350 9781588296351 |
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Datensatz im Suchindex
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| adam_text | Plant Proteomics
Methods and Protocols
Edited by
Hervé Thiellement
Michel Zivy
Catherine Damerval
UMRde
Valérie Méchin
UMR de Chimie Biologique, Thiverval Grignon, France
Plant Proteomics:
plant proteomics, each written by expert researchers from leading laboratories. Experimental
methodologies are explained in clear and complete detail for both the wet and dry laboratories.
The methods and protocols presented in this volume will inspire the novice as well as the expert
researcher to undertake new experimental endeavors in plant proteomics.
Chapters
protocols for protein extraction of peculiar tissues, such as cereal seeds, xylem, phloem sap, of
woody plants, and of cellular compartments, such as chloroplasts and mitochondria. These
methods rely on improvements to solubilization of proteins, which is the focus of Chapters
and
of intact proteins, mass spectrometry (MALDI-TOF and MS/MS) with proteins separated by 2D
gels, blue native IDgels, and Edman sequencing. Chapters24 to
area of analysis of post-translational modifications and protein-protein relationships. Attention
is also given to data that proteomics produces, including management
tools used for effective analysis.
FEATURES
Up-to-date protocols from the best laborato¬
ries in the field
Optimized procedures for extraction of
proteins from peculiar tissues
Characterization of sub-proteomes
Characterization of post-translational
modifications
Techniques for the coupling of mass spectrom¬
etry with 2DLC or LC
Discussion of the organization of proteomic
data into databases
Statistical tools for efficient analysis of the
abundance of proteomic data produced
Contents
Preface
Contributors
1
Valérie Méchin,
2
of Recalcitrant Plant Tissues
Mireille Faurobert,
3
Gérard Branlard
4
Julia
5
Christophe
6
for Proteome Analysis
Klaas
7
for Proteomic Analysis
Holger Eubel, Joshua L. Heazlewood, and A. Harvey Millar
8
Fernando
9
Setsuko Komatsu
10
Bonnie S. Watson and Lloyd W. Sumner
11
for Proteomic Analysis
Véronique
12
before Two-Dimensional Electrophoresis
Thierry Rabilloud,
and
vu
viii Contents
13 Two-Dimensional Electrophoresis
Walter
14
François
15 Two-Dimensional Differential In-Gel Electrophoresis (DIGE)
of Leaf and Roots of Lycopersicon esculentum
Matthew Keeler, Jessica Letarte, Emily Hattrup,
Fatimah
16
Michel Zivy
17
Kâre
18
Setsuko Komatsu
19
Identification of Proteins by MALDI-TOF
Nicolas
20
Chromatography-Tandem Mass Spectrometry
Luc Negroni
21
Mass Spectrometry of Proteins Extracted
from Rice Leaves and Roots
Linda
22
by GFC/IEC/SDS-PAGE and MALDI
Wojciech Szponarski,
Michel
23
Olivier
24
Maria V. Turkina and Alexander V. Vener
25
Anne-Catherine Fitchette, Olivia
and Muriel Bardor
26
of Protein Complexes in Plants
Jesco
and Hans-Peter
Contents ix
27 Electroelution
and Their Subsequent MALDI-TOF MS Analysis
Zhentian Lei, Ajith
and Lloyd W. Sumner
28
of Antigen-Antibody Interactions
Birgit
29
Tanja Feilner
Index
|
| adam_txt |
Plant Proteomics
Methods and Protocols
Edited by
Hervé Thiellement
Michel Zivy
Catherine Damerval
UMRde
Valérie Méchin
UMR de Chimie Biologique, Thiverval Grignon, France
Plant Proteomics:
plant proteomics, each written by expert researchers from leading laboratories. Experimental
methodologies are explained in clear and complete detail for both the wet and dry laboratories.
The methods and protocols presented in this volume will inspire the novice as well as the expert
researcher to undertake new experimental endeavors in plant proteomics.
Chapters
protocols for protein extraction of peculiar tissues, such as cereal seeds, xylem, phloem sap, of
woody plants, and of cellular compartments, such as chloroplasts and mitochondria. These
methods rely on improvements to solubilization of proteins, which is the focus of Chapters
and
of intact proteins, mass spectrometry (MALDI-TOF and MS/MS) with proteins separated by 2D
gels, blue native IDgels, and Edman sequencing. Chapters24 to
area of analysis of post-translational modifications and protein-protein relationships. Attention
is also given to data that proteomics produces, including management
tools used for effective analysis.
FEATURES
Up-to-date protocols from the best laborato¬
ries in the field
Optimized procedures for extraction of
proteins from peculiar tissues
Characterization of sub-proteomes
Characterization of post-translational
modifications
Techniques for the coupling of mass spectrom¬
etry with 2DLC or LC
Discussion of the organization of proteomic
data into databases
Statistical tools for efficient analysis of the
abundance of proteomic data produced
Contents
Preface
Contributors
1
Valérie Méchin,
2
of Recalcitrant Plant Tissues
Mireille Faurobert,
3
Gérard Branlard
4
Julia
5
Christophe
6
for Proteome Analysis
Klaas
7
for Proteomic Analysis
Holger Eubel, Joshua L. Heazlewood, and A. Harvey Millar
8
Fernando
9
Setsuko Komatsu
10
Bonnie S. Watson and Lloyd W. Sumner
11
for Proteomic Analysis
Véronique
12
before Two-Dimensional Electrophoresis
Thierry Rabilloud,
and
vu
viii Contents
13 Two-Dimensional Electrophoresis
Walter
14
François
15 Two-Dimensional Differential In-Gel Electrophoresis (DIGE)
of Leaf and Roots of Lycopersicon esculentum
Matthew Keeler, Jessica Letarte, Emily Hattrup,
Fatimah
16
Michel Zivy
17
Kâre
18
Setsuko Komatsu
19
Identification of Proteins by MALDI-TOF
Nicolas
20
Chromatography-Tandem Mass Spectrometry
Luc Negroni
21
Mass Spectrometry of Proteins Extracted
from Rice Leaves and Roots
Linda
22
by GFC/IEC/SDS-PAGE and MALDI
Wojciech Szponarski,
Michel
23
Olivier
24
Maria V. Turkina and Alexander V. Vener
25
Anne-Catherine Fitchette, Olivia
and Muriel Bardor
26
of Protein Complexes in Plants
Jesco
and Hans-Peter
Contents ix
27 Electroelution
and Their Subsequent MALDI-TOF MS Analysis
Zhentian Lei, Ajith
and Lloyd W. Sumner
28
of Antigen-Antibody Interactions
Birgit
29
Tanja Feilner
Index |
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| spelling | Plant proteomics methods and protocols ed. by Hervé Thiellement ... Totowa, NJ Humana Press 2007 XIII, 399 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Methods in molecular biology 355 Includes bibliographical references and index Protéines végétales Protéomique végétale Plant proteins Plant proteomics Genetik (DE-588)4071711-2 gnd rswk-swf Proteom (DE-588)4576155-3 gnd rswk-swf Proteomanalyse (DE-588)4596545-6 gnd rswk-swf Pflanzen (DE-588)4045539-7 gnd rswk-swf (DE-588)4143413-4 Aufsatzsammlung gnd-content Pflanzen (DE-588)4045539-7 s Proteomanalyse (DE-588)4596545-6 s DE-604 Proteom (DE-588)4576155-3 s Genetik (DE-588)4071711-2 s b DE-604 Thiellement, Hervé 1948- Sonstige (DE-588)1038595975 oth Methods in molecular biology 355 (DE-604)BV035362695 355 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014840417&sequence=000003&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Klappentext Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014840417&sequence=000004&line_number=0002&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Plant proteomics methods and protocols Methods in molecular biology Protéines végétales Protéomique végétale Plant proteins Plant proteomics Genetik (DE-588)4071711-2 gnd Proteom (DE-588)4576155-3 gnd Proteomanalyse (DE-588)4596545-6 gnd Pflanzen (DE-588)4045539-7 gnd |
| subject_GND | (DE-588)4071711-2 (DE-588)4576155-3 (DE-588)4596545-6 (DE-588)4045539-7 (DE-588)4143413-4 |
| title | Plant proteomics methods and protocols |
| title_auth | Plant proteomics methods and protocols |
| title_exact_search | Plant proteomics methods and protocols |
| title_exact_search_txtP | Plant proteomics methods and protocols |
| title_full | Plant proteomics methods and protocols ed. by Hervé Thiellement ... |
| title_fullStr | Plant proteomics methods and protocols ed. by Hervé Thiellement ... |
| title_full_unstemmed | Plant proteomics methods and protocols ed. by Hervé Thiellement ... |
| title_short | Plant proteomics |
| title_sort | plant proteomics methods and protocols |
| title_sub | methods and protocols |
| topic | Protéines végétales Protéomique végétale Plant proteins Plant proteomics Genetik (DE-588)4071711-2 gnd Proteom (DE-588)4576155-3 gnd Proteomanalyse (DE-588)4596545-6 gnd Pflanzen (DE-588)4045539-7 gnd |
| topic_facet | Protéines végétales Protéomique végétale Plant proteins Plant proteomics Genetik Proteom Proteomanalyse Pflanzen Aufsatzsammlung |
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