Basic methods for the biochemical lab: 23 figures and 86 tables
Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
Berlin [u.a.]
Springer
2006
|
| Ausgabe: | 1., engl. ed. |
| Schriftenreihe: | Springer Lab Manual
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XVIII, 251 S. graph. Darst. 235 mm x 155 mm |
| ISBN: | 3540327851 9783540327851 |
Internformat
MARC
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| 020 | |a 3540327851 |c Pb. : ca. EUR 42.75 (freier Pr.), ca. sfr 73.00 (freier Pr.) |9 3-540-32785-1 | ||
| 020 | |a 9783540327851 |9 978-3-540-32785-1 | ||
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| 084 | |a QU 25 |2 nlm | ||
| 084 | |a 570 |2 sdnb | ||
| 100 | 1 | |a Holtzhauer, Martin |e Verfasser |4 aut | |
| 240 | 1 | 0 | |a Biochemische Labormethoden |
| 245 | 1 | 0 | |a Basic methods for the biochemical lab |b 23 figures and 86 tables |c Martin Holtzhauer |
| 250 | |a 1., engl. ed. | ||
| 264 | 1 | |a Berlin [u.a.] |b Springer |c 2006 | |
| 300 | |a XVIII, 251 S. |b graph. Darst. |c 235 mm x 155 mm | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 0 | |a Springer Lab Manual | |
| 650 | 0 | 7 | |a Biochemie |0 (DE-588)4006777-4 |2 gnd |9 rswk-swf |
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| 650 | 0 | 7 | |a Methode |0 (DE-588)4038971-6 |2 gnd |9 rswk-swf |
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| 856 | 4 | 2 | |m Digitalisierung UB Regensburg |q application/pdf |u http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014832367&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |3 Inhaltsverzeichnis |
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Datensatz im Suchindex
| _version_ | 1804135406809120768 |
|---|---|
| adam_text | Table of Contents
Abbreviations
1
1.1
1.1.1
1.1.1.1
1.1.1.2
1.1.1.3
1.1.1.4
of Interfering Substances
1.1.2
1.1.3
1.1.4
1.1.5
1.1.5.1
1.1.5.2
1.1.6
1.1.7
1.2
1.2.1
and Protein Separation Procedure
1.2.2
1.2.3
1.2.4
1.2.5
1.3
1.3.1
1.3.2
1.3.3
1.4
1.5
2
2.1
2.1.1
2.1.2
(NuPAGE)
Table of Contents
2.1.3
to Weber,
2.1.4
for the Separation of Low Molecular Weight Proteins
2.1.5
for Proteins and Oligopeptides in the Range
of
2.1.6
2.1.7
atpH2
2.1.8
(Native PAGE)
2.1.9
(Native PAGE)
2.1.10
2.1.11
(2D-PAGE; IEF followed by SDS-PAGE)
2.1.11.1
2.1.11.2
(Acrylamide Gradient Gel)
2.2
2.2.1
2.2.2
2.2.3
2.3
2.3.1
2.3.1.1
2.3.1.2
2.3.2
2.3.3
2.4
2.4.1
2.4.1.1
2.4.1.2
2.4.1.3
with Bismarck Brown R
2.4.1.4
2.4.1.5
2.4.1.6
2.4.2
2.4.2.1
2.4.2.2
2.4.2.3
2.4.2.4
2.4.2.5
2.4.2.6
2.4.2.7
2.4.3
Table of Contents XI
2.4.4
2.4.4.1
2.4.4.2
2.4.5
2.4.5.1
2.4.5.2
2.4.5.3
2.5
2.5.1
from Polyacrylamide Gels
2.5.2
2.5.3
(Electroblotting; Western Blot): Semi-dry Blotting
2.5.4
(Immunoblotting)
2.5.4.1
2.5.4.2
2.5.5
2.5.5.1
2.5.5.2
2.5.6
After Electrotransfer
2.5.7
2.5.8
2.5.9
2.6
2.7
3
3.1
3.1.1
(TLC
3.1.2
3.1.3
3.1.4
3.1.5
3.2
3.3
Size-Exclusion Chromatography, SEC)
3.3.1
3.3.2
3.3.3
3.3.4
3.3.5
3.3.6
by Gel Filtration ( Desalting )
3.4
3.4.1
XII
3.4.2
3.4.3 Sample Application....................................... 104
3.4.4 Elution ................................................. 105
3.4.5
3.4.6
of
3.5 Hydrophobie
3.5.1
3.5.2 Elution ................................................. 108
3.5.3 Regeneration............................................ 108
3.5.4
3.6
3.6.1
of Polysaccharide-Based Supports
3.6.1.1
3.6.2
3.6.2.1
Spacers with 2,4,6-Trinitrobenzene Sulfonic Acid
3.6.2.2
3.6.2.3
3.6.2.4
3.6.2.5
on Immobilized Antibodies (Immunoaffinity
Chromatography, IAC)
3.6.2.6
on Protein-A Supports
3.6.3
3.6.3.1
3.6.4
3.6.5
3.6.6
Chromatography)
3.6.7
(Biotin-Avidin/Streptavidin System)
3.6.8
of Proteins Containing His6-Tag
3.7
3.7.1
3.7.2
3.7.3
3.7.4
3.7.5 Ultrafiltration............................................ 127
4
4.1
4.1.1
with Additional Free SH Groups
4.1.2
Table of
4.1.3
4-(N-Maleimidomethyl)-Cyclohexane-l-Carbonic Acid
N-Hydroxysuccinimide Ester (SMCC)
4.1.4
(Coupling via SH Groups)
4.1.4.1
4.1.5
with
(EDAC, EDC)
4.1.6
by
4.1.7
Using Glutaraldehyde (Two-Step Procedure)
4.1.8
4.1.9
(Glutaraldehyde Protocol)
4.1.9.1
from Calf Intestine
4.1.10
4.1.11
4.1.11.1
4.1.11.2
4.2
4.3
4.4
4.4.1
4.5
4.6
4.6.1
4.6.2
4.6.3
4.7 Heidelberger
4.8
4.8.1
4.8.2
4.8.3 Immunodiffusion........................................ 152
4.8.4
4.9
4.10
4.11
4.12
4.13
4.13.1
4.13.2
4.13.3
Xiv
5
5.1
5.2
5.3
5.3.1
Isolation of Liver Cell Nuclei
5.3.2
of Surface Membranes (Sarcolemma, SL) of Heart Muscle Cells
5.3.2.1
Ouabain-Sensitive Na,K-ATPase
5.3.2.2
on Surface Membranes
5.3.2.3
and Association Kinetics of the DHP Receptors
5.3.3
Gradient Centrifugation
5.3.4
5.3.5
5.3.5.1
in CsCl Gradients
5.3.5.2
5.3.5.3
6
6.1
6.2
6.3
6.4
6.4.1
6.4.2
6.5
7
7.1
7.2
7.3 pH
7.4
7.4.1
7.4.2
and Organ
7.4.3 pH
7.4.4
8
8.1
8.2
8.3
8.4
Table of
8.5
8.6
8.7
8.8
8.9
8.10
8.11
8.12
9
9.1
9.1.1
9.1.2
9.1.3
9.2
9.2.1
9.2.2
9.2.3
9.3
9.4
9.4.1
9.4.2
9.4.3
9.4.4
Subject Index
|
| adam_txt |
Table of Contents
Abbreviations
1
1.1
1.1.1
1.1.1.1
1.1.1.2
1.1.1.3
1.1.1.4
of Interfering Substances
1.1.2
1.1.3
1.1.4
1.1.5
1.1.5.1
1.1.5.2
1.1.6
1.1.7
1.2
1.2.1
and Protein Separation Procedure
1.2.2
1.2.3
1.2.4
1.2.5
1.3
1.3.1
1.3.2
1.3.3
1.4
1.5
2
2.1
2.1.1
2.1.2
(NuPAGE)
Table of Contents
2.1.3
to Weber,
2.1.4
for the Separation of Low Molecular Weight Proteins
2.1.5
for Proteins and Oligopeptides in the Range
of
2.1.6
2.1.7
atpH2
2.1.8
(Native PAGE)
2.1.9
(Native PAGE)
2.1.10
2.1.11
(2D-PAGE; IEF followed by SDS-PAGE)
2.1.11.1
2.1.11.2
(Acrylamide Gradient Gel)
2.2
2.2.1
2.2.2
2.2.3
2.3
2.3.1
2.3.1.1
2.3.1.2
2.3.2
2.3.3
2.4
2.4.1
2.4.1.1
2.4.1.2
2.4.1.3
with Bismarck Brown R
2.4.1.4
2.4.1.5
2.4.1.6
2.4.2
2.4.2.1
2.4.2.2
2.4.2.3
2.4.2.4
2.4.2.5
2.4.2.6
2.4.2.7
2.4.3
Table of Contents XI
2.4.4
2.4.4.1
2.4.4.2
2.4.5
2.4.5.1
2.4.5.2
2.4.5.3
2.5
2.5.1
from Polyacrylamide Gels
2.5.2
2.5.3
(Electroblotting; Western Blot): Semi-dry Blotting
2.5.4
(Immunoblotting)
2.5.4.1
2.5.4.2
2.5.5
2.5.5.1
2.5.5.2
2.5.6
After Electrotransfer
2.5.7
2.5.8
2.5.9
2.6
2.7
3
3.1
3.1.1
(TLC
3.1.2
3.1.3
3.1.4
3.1.5
3.2
3.3
Size-Exclusion Chromatography, SEC)
3.3.1
3.3.2
3.3.3
3.3.4
3.3.5
3.3.6
by Gel Filtration ("Desalting")
3.4
3.4.1
XII
3.4.2
3.4.3 Sample Application. 104
3.4.4 Elution . 105
3.4.5
3.4.6
of
3.5 Hydrophobie
3.5.1
3.5.2 Elution . 108
3.5.3 Regeneration. 108
3.5.4
3.6
3.6.1
of Polysaccharide-Based Supports
3.6.1.1
3.6.2
3.6.2.1
Spacers with 2,4,6-Trinitrobenzene Sulfonic Acid
3.6.2.2
3.6.2.3
3.6.2.4
3.6.2.5
on Immobilized Antibodies (Immunoaffinity
Chromatography, IAC)
3.6.2.6
on Protein-A Supports
3.6.3
3.6.3.1
3.6.4
3.6.5
3.6.6
Chromatography)
3.6.7
(Biotin-Avidin/Streptavidin System)
3.6.8
of Proteins Containing His6-Tag
3.7
3.7.1
3.7.2
3.7.3
3.7.4
3.7.5 Ultrafiltration. 127
4
4.1
4.1.1
with Additional Free SH Groups
4.1.2
Table of
4.1.3
4-(N-Maleimidomethyl)-Cyclohexane-l-Carbonic Acid
N-Hydroxysuccinimide Ester (SMCC)
4.1.4
(Coupling via SH Groups)
4.1.4.1
4.1.5
with
(EDAC, EDC)
4.1.6
by
4.1.7
Using Glutaraldehyde (Two-Step Procedure)
4.1.8
4.1.9
(Glutaraldehyde Protocol)
4.1.9.1
from Calf Intestine
4.1.10
4.1.11
4.1.11.1
4.1.11.2
4.2
4.3
4.4
4.4.1
4.5
4.6
4.6.1
4.6.2
4.6.3
4.7 Heidelberger
4.8
4.8.1
4.8.2
4.8.3 Immunodiffusion. 152
4.8.4
4.9
4.10
4.11
4.12
4.13
4.13.1
4.13.2
4.13.3
Xiv
5
5.1
5.2
5.3
5.3.1
Isolation of Liver Cell Nuclei
5.3.2
of Surface Membranes (Sarcolemma, SL) of Heart Muscle Cells
5.3.2.1
Ouabain-Sensitive Na,K-ATPase
5.3.2.2
on Surface Membranes
5.3.2.3
and Association Kinetics of the DHP Receptors
5.3.3
Gradient Centrifugation
5.3.4
5.3.5
5.3.5.1
in CsCl Gradients
5.3.5.2
5.3.5.3
6
6.1
6.2
6.3
6.4
6.4.1
6.4.2
6.5
7
7.1
7.2
7.3 pH
7.4
7.4.1
7.4.2
and Organ
7.4.3 pH
7.4.4
8
8.1
8.2
8.3
8.4
Table of
8.5
8.6
8.7
8.8
8.9
8.10
8.11
8.12
9
9.1
9.1.1
9.1.2
9.1.3
9.2
9.2.1
9.2.2
9.2.3
9.3
9.4
9.4.1
9.4.2
9.4.3
9.4.4
Subject Index |
| any_adam_object | 1 |
| any_adam_object_boolean | 1 |
| author | Holtzhauer, Martin |
| author_facet | Holtzhauer, Martin |
| author_role | aut |
| author_sort | Holtzhauer, Martin |
| author_variant | m h mh |
| building | Verbundindex |
| bvnumber | BV021617248 |
| classification_rvk | WC 4150 |
| classification_tum | CHE 810f |
| ctrlnum | (OCoLC)633978024 (DE-599)BVBBV021617248 |
| discipline | Biologie Chemie |
| discipline_str_mv | Biologie Chemie |
| edition | 1., engl. ed. |
| format | Book |
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| id | DE-604.BV021617248 |
| illustrated | Illustrated |
| index_date | 2024-07-02T14:52:35Z |
| indexdate | 2024-07-09T20:40:00Z |
| institution | BVB |
| isbn | 3540327851 9783540327851 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-014832367 |
| oclc_num | 633978024 |
| open_access_boolean | |
| owner | DE-91G DE-BY-TUM DE-M49 DE-BY-TUM DE-19 DE-BY-UBM DE-634 DE-11 DE-578 |
| owner_facet | DE-91G DE-BY-TUM DE-M49 DE-BY-TUM DE-19 DE-BY-UBM DE-634 DE-11 DE-578 |
| physical | XVIII, 251 S. graph. Darst. 235 mm x 155 mm |
| publishDate | 2006 |
| publishDateSearch | 2006 |
| publishDateSort | 2006 |
| publisher | Springer |
| record_format | marc |
| series2 | Springer Lab Manual |
| spelling | Holtzhauer, Martin Verfasser aut Biochemische Labormethoden Basic methods for the biochemical lab 23 figures and 86 tables Martin Holtzhauer 1., engl. ed. Berlin [u.a.] Springer 2006 XVIII, 251 S. graph. Darst. 235 mm x 155 mm txt rdacontent n rdamedia nc rdacarrier Springer Lab Manual Biochemie (DE-588)4006777-4 gnd rswk-swf Labortechnik (DE-588)4123602-6 gnd rswk-swf Laboratorium (DE-588)4033927-0 gnd rswk-swf Methode (DE-588)4038971-6 gnd rswk-swf Biochemie (DE-588)4006777-4 s Laboratorium (DE-588)4033927-0 s Methode (DE-588)4038971-6 s DE-604 Labortechnik (DE-588)4123602-6 s 1\p DE-604 Digitalisierung UB Regensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014832367&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | Holtzhauer, Martin Basic methods for the biochemical lab 23 figures and 86 tables Biochemie (DE-588)4006777-4 gnd Labortechnik (DE-588)4123602-6 gnd Laboratorium (DE-588)4033927-0 gnd Methode (DE-588)4038971-6 gnd |
| subject_GND | (DE-588)4006777-4 (DE-588)4123602-6 (DE-588)4033927-0 (DE-588)4038971-6 |
| title | Basic methods for the biochemical lab 23 figures and 86 tables |
| title_alt | Biochemische Labormethoden |
| title_auth | Basic methods for the biochemical lab 23 figures and 86 tables |
| title_exact_search | Basic methods for the biochemical lab 23 figures and 86 tables |
| title_exact_search_txtP | Basic methods for the biochemical lab 23 figures and 86 tables |
| title_full | Basic methods for the biochemical lab 23 figures and 86 tables Martin Holtzhauer |
| title_fullStr | Basic methods for the biochemical lab 23 figures and 86 tables Martin Holtzhauer |
| title_full_unstemmed | Basic methods for the biochemical lab 23 figures and 86 tables Martin Holtzhauer |
| title_short | Basic methods for the biochemical lab |
| title_sort | basic methods for the biochemical lab 23 figures and 86 tables |
| title_sub | 23 figures and 86 tables |
| topic | Biochemie (DE-588)4006777-4 gnd Labortechnik (DE-588)4123602-6 gnd Laboratorium (DE-588)4033927-0 gnd Methode (DE-588)4038971-6 gnd |
| topic_facet | Biochemie Labortechnik Laboratorium Methode |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014832367&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT holtzhauermartin biochemischelabormethoden AT holtzhauermartin basicmethodsforthebiochemicallab23figuresand86tables |