T-cell epitope spreading to myelin oligodendrocyte glycoprotein in HLA-DR4 transgenic mice experimental autoimmune encephalomyelitis:
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
2005
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Ulm, Univ., Diss., 2005 |
| Beschreibung: | 66 Bl. Ill., graph. Darst. 30 cm |
Internformat
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| 245 | 1 | 0 | |a T-cell epitope spreading to myelin oligodendrocyte glycoprotein in HLA-DR4 transgenic mice experimental autoimmune encephalomyelitis |c vorgelegt von Juliane Klehmet |
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Datensatz im Suchindex
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| adam_text | 3
Table of contents
1. Abbreviations 5
2. Introduction 8
2.1 EAE is the animal model of multiple sclerosis 9
2.2 Pathogenesis of multiple sclerosis/EAE 9
2.3 The role of epitope spreading 11
2.4 The role of genetic factors 12
2.5 Myelin oligodendrocyte glycoprotein 13
2.6 Aims of the study 14
3. Materials and Methods 15
3.1 Mice, antigens, and treatments 15
3.2 Cell preparations and T cell separation 16
3.3 Cytokine measurements by ELISPOT and computer assisted
ELISPOT image analysis 18
3.4 Immunofluorescent labelling of surface antigens 20
3.5 Evaluation of clinical disease 21
3.6 Binding predictions of MOG sequences for HLA DRB1*0401 21
3.7 In vitro peptide binding studies 22
4. Results 24
4.1 Intra and intermolecular epitope spreading induction
of EAE with MOG97 108 in HLA DR4 transgenic mice 24
4.2 MOG181 200 has a high binding affinity for HLA DR4 (DRB1*0401) 31
4.3 MOGi79 i9i specific T cells are encephalitogenic 34
4
4.4. No intra and intermolecular epitope spreading to MOG or MBP
after immunization with the spreading epitope MOG 179 191 37
4.5 The spreading epitope MOG179.191 is presented by human APCs 40
5. Discussion 42
5.1. Creating of an appropriate mouse model 43
5.2. Demonstration of epitope spreading in the HLA DR4 tg. mice 44
5.3. Evaluation of the mechanism epitope spreading 46
5.4. Clinical Relevance and tasks for the future 47
6. Summary 50
7. Zusammenfassung 51
8. References 52
9. Danksagung 64
10. Curriculum vitae 65
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| adam_txt |
3
Table of contents
1. Abbreviations 5
2. Introduction 8
2.1 EAE is the animal model of multiple sclerosis 9
2.2 Pathogenesis of multiple sclerosis/EAE 9
2.3 The role of epitope spreading 11
2.4 The role of genetic factors 12
2.5 Myelin oligodendrocyte glycoprotein 13
2.6 Aims of the study 14
3. Materials and Methods 15
3.1 Mice, antigens, and treatments 15
3.2 Cell preparations and T cell separation 16
3.3 Cytokine measurements by ELISPOT and computer assisted
ELISPOT image analysis 18
3.4 Immunofluorescent labelling of surface antigens 20
3.5 Evaluation of clinical disease 21
3.6 Binding predictions of MOG sequences for HLA DRB1*0401 21
3.7 In vitro peptide binding studies 22
4. Results 24
4.1 Intra and intermolecular epitope spreading induction
of EAE with MOG97 108 in HLA DR4 transgenic mice 24
4.2 MOG181 200 has a high binding affinity for HLA DR4 (DRB1*0401) 31
4.3 MOGi79 i9i specific T cells are encephalitogenic 34
4
4.4. No intra and intermolecular epitope spreading to MOG or MBP
after immunization with the spreading epitope MOG 179 191 37
4.5 The spreading epitope MOG179.191 is presented by human APCs 40
5. Discussion 42
5.1. Creating of an appropriate mouse model 43
5.2. Demonstration of epitope spreading in the HLA DR4 tg. mice 44
5.3. Evaluation of the mechanism epitope spreading 46
5.4. Clinical Relevance and tasks for the future 47
6. Summary 50
7. Zusammenfassung 51
8. References 52
9. Danksagung 64
10. Curriculum vitae 65 |
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| author | Klehmet, Juliane 1974- |
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| author_facet | Klehmet, Juliane 1974- |
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| ctrlnum | (OCoLC)255529399 (DE-599)BVBBV021576714 |
| dewey-full | 616.8 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 616 - Diseases |
| dewey-raw | 616.8 |
| dewey-search | 616.8 |
| dewey-sort | 3616.8 |
| dewey-tens | 610 - Medicine and health |
| discipline | Medizin |
| discipline_str_mv | Medizin |
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| index_date | 2024-07-02T14:40:22Z |
| indexdate | 2024-07-09T20:39:02Z |
| institution | BVB |
| language | English |
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| spelling | Klehmet, Juliane 1974- Verfasser (DE-588)130955159 aut T-cell epitope spreading to myelin oligodendrocyte glycoprotein in HLA-DR4 transgenic mice experimental autoimmune encephalomyelitis vorgelegt von Juliane Klehmet 2005 66 Bl. Ill., graph. Darst. 30 cm txt rdacontent n rdamedia nc rdacarrier Ulm, Univ., Diss., 2005 (DE-588)4113937-9 Hochschulschrift gnd-content HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014792446&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Klehmet, Juliane 1974- T-cell epitope spreading to myelin oligodendrocyte glycoprotein in HLA-DR4 transgenic mice experimental autoimmune encephalomyelitis |
| subject_GND | (DE-588)4113937-9 |
| title | T-cell epitope spreading to myelin oligodendrocyte glycoprotein in HLA-DR4 transgenic mice experimental autoimmune encephalomyelitis |
| title_auth | T-cell epitope spreading to myelin oligodendrocyte glycoprotein in HLA-DR4 transgenic mice experimental autoimmune encephalomyelitis |
| title_exact_search | T-cell epitope spreading to myelin oligodendrocyte glycoprotein in HLA-DR4 transgenic mice experimental autoimmune encephalomyelitis |
| title_exact_search_txtP | T-cell epitope spreading to myelin oligodendrocyte glycoprotein in HLA-DR4 transgenic mice experimental autoimmune encephalomyelitis |
| title_full | T-cell epitope spreading to myelin oligodendrocyte glycoprotein in HLA-DR4 transgenic mice experimental autoimmune encephalomyelitis vorgelegt von Juliane Klehmet |
| title_fullStr | T-cell epitope spreading to myelin oligodendrocyte glycoprotein in HLA-DR4 transgenic mice experimental autoimmune encephalomyelitis vorgelegt von Juliane Klehmet |
| title_full_unstemmed | T-cell epitope spreading to myelin oligodendrocyte glycoprotein in HLA-DR4 transgenic mice experimental autoimmune encephalomyelitis vorgelegt von Juliane Klehmet |
| title_short | T-cell epitope spreading to myelin oligodendrocyte glycoprotein in HLA-DR4 transgenic mice experimental autoimmune encephalomyelitis |
| title_sort | t cell epitope spreading to myelin oligodendrocyte glycoprotein in hla dr4 transgenic mice experimental autoimmune encephalomyelitis |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014792446&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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