Ebola and Marburg viruses: molecular and cellular biology
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
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Wymondham
Horizon Bioscience
2004
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| Schlagworte: | |
| Online-Zugang: | Table of contents Inhaltsverzeichnis |
| Beschreibung: | X, 369 S. Ill., graph. Darst. |
| ISBN: | 0954523237 |
Internformat
MARC
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| 245 | 1 | 0 | |a Ebola and Marburg viruses |b molecular and cellular biology |c ed. by: Hans-Dieter Klenk ... |
| 264 | 1 | |a Wymondham |b Horizon Bioscience |c 2004 | |
| 300 | |a X, 369 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 650 | 7 | |a Ebolavirus |2 gtt | |
| 650 | 7 | |a Marburgvirus |2 gtt | |
| 650 | 4 | |a Marburg virus | |
| 650 | 4 | |a Marburg virus disease | |
| 650 | 4 | |a Ebola virus disease | |
| 650 | 4 | |a Molecular virology | |
| 650 | 0 | 7 | |a Ebola-Virus |0 (DE-588)4233551-6 |2 gnd |9 rswk-swf |
| 689 | 0 | 0 | |a Ebola-Virus |0 (DE-588)4233551-6 |D s |
| 689 | 0 | |C b |5 DE-604 | |
| 700 | 1 | |a Klenk, Hans-Dieter |d 1938-2021 |e Sonstige |0 (DE-588)136121721 |4 oth | |
| 856 | 4 | |u http://www.loc.gov/catdir/toc/fy053/2004351692.html |3 Table of contents | |
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Datensatz im Suchindex
| _version_ | 1804135339311235072 |
|---|---|
| adam_text | Contents
Frontispiece ii
Dedication v
Acknowledgements vii
Figures and Tables xiii
Preface xxi
Chapter 1. Introduction 1
Viruses and Virology 1
Filoviruses 4
Filoviruses: A Review of Their History 5
Filoviruses: Taxonomy 8
Chapter 2. Materials and Methods 11
Viruses 11
Animals and Cell Cultures 12
Electron Microscopy Sample Preparation 15
Summary 18
ix
Ebola and Marburg Viruses
Chapter 3. Morphology of Filoviruses 19
Filoviral Shapes 19
General Structure of Filoviruses 23
Structure and Function of Filoviral Proteins 24
Infection: Penetration 26
Infection: Morphology of Viral Replication 32
Infection: Viral Budding 42
Conclusion 53
Chapter 4. Filoviral Infections 57
Filoviral Reservoirs 57
Transmission Between Humans 60
Experimental Studies of Filoviral Infection 61
Transmission Mode and Disease 61
Primary Target Cells 63
Lymph Nodes 63
Injection Site Localization 64
Macrophage Cells and Susceptibility 66
Aerosol Infection 70
Secondary Target Cells 71
Other Target Cells 77
Conclusion 80
Chapter 5. Microscopic Pathology over the
Course of Filoviral Infection 85
Experimental Design 86
Filoviral Reproduction 88
Blood System 88
Liver 89
Spleen 97
Kidneys 97
Lungs 97
Adrenals 100
Heart 100
Digestive Tract 100
Summary of Target Cells and Target Organs 101
Contents xi
Chapter 6. Blood Disorders in Filoviral Infections 105
Clinical Manifestations 105
Morphologic Observations 107
Time Course of Pathomorphological Changes
in the Blood System 108
Species Related Differences in
EBOV Infected Monkeys 118
Possible Mechanisms 121
Blood Cell Counts 125
Conclusion 128
Chapter 7. Time Course of the Pathological Changes
in Filovirus lnfected Animals 131
Liver 132
Pathological Changes 132
Inflammation 137
Spleen 140
Kidneys 145
Lungs 151
Adrenals 153
Heart 156
Digestive Tract 157
Summary 158
Chapter 8. Immunopathology 159
Filoviral Reproduction In Lymphatic Tissue 160
Pathological Changes 161
Formation of Lymphocytes 163
Lymphoid Depletion 163
Damage of Macrophages, Stromal,
and Dendritic Cells 168
Other Pathological Changes 170
Lack of Inflammation 173
Immune Responses in Human Filoviral Infections 173
Possible Mechanisms of Immunity Impairment
and Directions of Future Research 174
Ebola and Marburg Viruses
Chapter 9. Conclusions 177
Filoviral Infection 177
Disease and Pathology 179
Inflammatory Response 180
Animal Models for Filoviral Studies 181
What Directly Causes Rapid Death
in Filoviral Infections? 182
Crucial Role of the Macrophages 183
Systemic Aspects of Fatal EBOV
and MARV Infections 184
References 187
Index 205
Figures and Tables
NOTE: In the photographs accompanying the text, numbers
and an adjacent legend identify pertinent items. For the
sake of clarity, when there are several items that illustrate
the same subject, only a few examples are identified.
FIGURES
Figure 2 1. An electron microscope photograph of
MARV made from an ultrathin section
(magnification 87,500) 17
Figure 2 2. An electron microscope picture of
MARV particles negatively stained by uranyl
acetate (magnification 120,000) 17
Figure 3 1. Various MARV structures are visible at
different magnifications in these three
photos made of ultrathin sections 20,21
Figure 3 2. Various shapes of MARV virions are
apparent in electron microscope pictures
of negatively stained preparations
(magnification 120,000) 22
Figure 3 3. Various filoviral shapes negatively stained
by uranyl acetate (magnification 19,000) .... 23
Figure 3 4. Schematic of a filovirus (adapted from
Feldmann, 1999) 25
Figure 3 5. Virus entry into the cell by receptor mediated
endocytosis and by direct fusion with the
plasma membrane 28
Figure 3 6. Patterns of receptor mediated endocytosis of
MARV; association of the virus with
clathrin coated pits 30
Figure 3 7. The first evidence of MARV infection is
the accumulation of viral material,
which sometimes appears as a
sponge like material 34, 35
Figure 3 8. Various sections showing the formation
and appearance of MARV nucleocapsids .... 36
Figure 3 9. Schematic of formation of
MARV inclusions 37
Figure 3 10. MARV inclusions in Vero cells 38, 39
Figure 3 11. Nucleocapsids of ZEBOV in longitudinal
and cross sectional views 40
Figure 3 12. ZEBOV inclusion composed of helices 41
Figure 3 13. MARV budding (magnification 116,000) 43
Figure 3 14. Different patterns of the beginning stages
of MARV budding 44, 45
Figure 3 15. Variations in budding that lead to
ring shaped virions for MARV 45
Figure 3 16. A freeze etching picture of a MARV suspension
clearly showing rod and disc shaped virions
(magnification 80,000) 46
Figure 3 17. Schematic of the viral budding process
for filoviruses 47
Figure 3 18. ZEBOV infected cell of chick embryo
(magnification 10,700) 48
Figure 3 19. The development of net like structures in
filovirus infected cells 51, 52
Figure 4 1. MARV replication in a peritoneal macrophage
of a guinea pig as seen in an ultrathin
section of the cells of peritoneal exudate
obtained at 72 h post inoculation
(magnification 31,000) 65
Figure 4 2. An ultrathin section of a Kuppfer (macrophage)
cell in a liver of green monkey infected by
ZEBOV, day 3 post inoculation
(magnification 20,000) 69
Figure 4 3. Ultrathin sections showing replication
of filoviruses in hepatocytes of
green monkeys 72
Figure 4 4. Ultrathin section of adrenal cortex showing
replication of ZEBOV in green monkey
adrenal cortical cells (magnification 7,000) ... 73
Figure 4 5. Ultrathin sections showing replication
of filoviruses in green monkey
endothelial cells 74, 75
Figure 4 6. Ultrathin sections showing replication
of ZEBOV in fibroblasts of rhesus
monkey 76, 77
Figure 4 7. ZEBOV replication occurs rarely in the
airway cells of green monkeys IS, 79
Figure 4 8. Sequential steps of filoviral dissemination
during infections in guinea pigs
and monkeys 83
Figure 5 1. An ultrathin section of macrophage
infected with ZEBOV in a green monkey s
lymphatic node (magnification 12,000) 89
Figure 5 2. Ultrathin section of a liver of guinea pig
sampled 7 days after inoculation with
MARV (magnification 11,000) 90
Figure 5 3. Light and electron microscope views of
ZEBOV infected liver in a baboon 91
Figure 5 4. The changes in liver pathology for ZEBOV
and MARV infections 92, 93
Figure 5 5. The ultrathin section of the liver of an
infected guinea pig with the replication
of MARV in the endothelial cell
(magnification 11,300) 94
Figure 5 6. Ultrathin sections highlighting differences
from the normal replication of ZEBOV
in the hepatocyte of a rhesus monkey 96
Figure 5 7. An ultrathin section of a green monkey s
lung showing replication of ZEBOV
in a circulating macrophage
(magnification 17,000) 99
Figure 5 8. An ultrathin section of the heart of a
green monkey infected with ZEBOV shows
replication in a macrophage located in
interstitial tissue (magnification 12,500) .... 101
Figure 5 9. The schematic depicts the progression of
ZEBOV and MARV infection of tissues
and organs in fatally infected monkeys
and guinea pigs 102
Figure 6 1. Light microscope photographs showing
changes in the blood circulation in the liver,
kidney, and lung tissues in ZEBOV infected
monkeys 109
Figure 6 2. Two different views of microcirculation
damage in tissues 110, 111
Figure 6 3. An ultrathin section highlighting the
accumulation of neutrophils in an
alveolar capillary in a ZEBOV infected
green monkey (magnification 7,000) 112
Figure 6 4. A small arteriole in the spleen of a rhesus
monkey infected with ZEBOV
(magnification 10,000) 113
Figure 6 5. Epithelial cells in intestines are not
infected even at the terminal stages
of filoviral disease 114
Figure 6 6. The relation of fibrin clots to the surface of a
macrophage in a green monkey liver 116
Figure 6 7. Light and electron microscope pictures of
ZEBOV infected baboon adrenal gland tissue
that demonstrates prominent hemorrhages . . 117
Figure 6 8. An ultrathin section of a baboon spleen
showing diapedesis of erythrocytes into
interstitial tissue (magnification 14,000) .... 121
Figure 6 9. The bone marrow tissue from a MARV
infected guinea pig 127
Figure 6 10. Events leading to blood system damage
in ZEBOV and MARV infected animals .... 130
Figure 7 1. A section of the liver of guinea pig
infected with MARV before death
(magnification 6,000) 133
Figure 7 2. Fibrin thrombus clogging a hepatic sinusoid
in a rhesus monkey infected with ZEBOV
(magnification 10,500) 135
Figure 7 3. The effects of hemorrhages in the liver
of baboon infected with ZEBOV
(magnification 7,000) 136
Figure 7 4. Image from a light microscope showing the
liver from a guinea pig infected with
unadapted ZEBOV 138
Figure 7 5. The close contact of leukocytes with infected
hepatocytes from the liver of a guinea
pig infected by aerosolized MARV
(magnification 6,200) 139
Figure 7 6. The thrombus with various blood cells
in a splenic red pulp vessel
(magnification 8,000) 141
Figure 7 7. A ZEBOV infected macrophage in the
splenic red pulp of a green monkey
(magnification 16,000) 142
Figure 7 8. A fibrin thrombus in the lumen of a blood
vessel in the red pulp of a ZEBOV infected
green monkey (magnification 6,000) 143
Figure 7 9. Part of a green monkey s splenic red pulp
illustrating the main features of spleen
damage in the terminal stages of ZEBOV
infection (magnification 3,500) 144
Figure 7 10. Renal damage in green monkeys infected
with MARV via a semithin light microscope
section and an ultrathin electron
microscope section 147, 148
Figure 7 11. Two variations of the type of localized
renal tissue lesions that are seen in
filovirus infected animals 149, 150
Figure 7 12. Damage to the air blood barrier in a
ZEBOV infected green monkey
(magnification 4,000) 152
Figure 7 13. ZEBOV reproduction in the adrenal
glands of a green monkey 154, 155
Figure 7 14. A section from the heart of green
monkey infected with ZEBOV
(magnification 21,000) 157
Figure 8 1. A ZEBOV infected macrophage in the
lymphatic node of green monkey with
all the hallmarks of a filoviral infection
(magnification 16,000) 162
Figure 8 2. Two light microscope photographs of the
cortical portion of the inguinal lymphatic
nodes of a green monkey 164, 165
Figure 8 3. Light microscope photographs of a semithin
section of the cortical zone of a lymphatic
node from an infected monkey 166
Figure 8 4. Apoptosis of lymphocytes and plasma cells
in an infected green monkey s lymphatic
node, sampled before death
(magnification 8,500) 167
Figure 8 5. Swollen stromal (reticular) cell from a
MARV infected green monkey
(magnification 7,500) 169
Figure 8 6. Viral particles near the protrusions of
dendritic cell (magnification 27,700) 171
Figure 8 7. Light microscope photograph of severely
altered lymphatic node of baboon infected
with ZEBOV showing damage of
lymphatic tissue and hemorrhages 172
TABLES
Table 2 1. Description of the filoviral experiments in
animals that provided the samples for
microscopic studies 13
Table 3 1. Proposed functions of the filoviral proteins .... 25
Table 3 2. Summary of differences in morphological
characteristics for MARV (strain Popp) and
ZEBOV infections 55
Table 4 1. The relation between dose and inoculation
mode on the time of filoviral detection
in blood (viremia) 62
Table 4 2. MARV titers (PFU) in peritoneal macrophage
cultured medium from various animals 67
Table 4 3. MARV titers (PFU) in peritoneal macrophage
cultured medium from baboons 67
Table 5 1. Summary of experimental design 87
Table 6 1. Species specific characteristics of
ZEBOV infected monkeys 119
|
| adam_txt |
Contents
Frontispiece ii
Dedication v
Acknowledgements vii
Figures and Tables xiii
Preface xxi
Chapter 1. Introduction 1
Viruses and Virology 1
Filoviruses 4
Filoviruses: A Review of Their History 5
Filoviruses: Taxonomy 8
Chapter 2. Materials and Methods 11
Viruses 11
Animals and Cell Cultures 12
Electron Microscopy Sample Preparation 15
Summary 18
ix
Ebola and Marburg Viruses
Chapter 3. Morphology of Filoviruses 19
Filoviral Shapes 19
General Structure of Filoviruses 23
Structure and Function of Filoviral Proteins 24
Infection: Penetration 26
Infection: Morphology of Viral Replication 32
Infection: Viral Budding 42
Conclusion 53
Chapter 4. Filoviral Infections 57
Filoviral Reservoirs 57
Transmission Between Humans 60
Experimental Studies of Filoviral Infection 61
Transmission Mode and Disease 61
Primary Target Cells 63
Lymph Nodes 63
Injection Site Localization 64
Macrophage Cells and Susceptibility 66
Aerosol Infection 70
Secondary Target Cells 71
Other Target Cells 77
Conclusion 80
Chapter 5. Microscopic Pathology over the
Course of Filoviral Infection 85
Experimental Design 86
Filoviral Reproduction 88
Blood System 88
Liver 89
Spleen 97
Kidneys 97
Lungs 97
Adrenals 100
Heart 100
Digestive Tract 100
Summary of Target Cells and Target Organs 101
Contents xi
Chapter 6. Blood Disorders in Filoviral Infections 105
Clinical Manifestations 105
Morphologic Observations 107
Time Course of Pathomorphological Changes
in the Blood System 108
Species Related Differences in
EBOV Infected Monkeys 118
Possible Mechanisms 121
Blood Cell Counts 125
Conclusion 128
Chapter 7. Time Course of the Pathological Changes
in Filovirus lnfected Animals 131
Liver 132
Pathological Changes 132
Inflammation 137
Spleen 140
Kidneys 145
Lungs 151
Adrenals 153
Heart 156
Digestive Tract 157
Summary 158
Chapter 8. Immunopathology 159
Filoviral Reproduction In Lymphatic Tissue 160
Pathological Changes 161
Formation of Lymphocytes 163
Lymphoid Depletion 163
Damage of Macrophages, Stromal,
and Dendritic Cells 168
Other Pathological Changes 170
Lack of Inflammation 173
Immune Responses in Human Filoviral Infections 173
Possible Mechanisms of Immunity Impairment
and Directions of Future Research 174
Ebola and Marburg Viruses
Chapter 9. Conclusions 177
Filoviral Infection 177
Disease and Pathology 179
Inflammatory Response 180
Animal Models for Filoviral Studies 181
What Directly Causes Rapid Death
in Filoviral Infections? 182
Crucial Role of the Macrophages 183
Systemic Aspects of Fatal EBOV
and MARV Infections 184
References 187
Index 205
Figures and Tables
NOTE: In the photographs accompanying the text, numbers
and an adjacent legend identify pertinent items. For the
sake of clarity, when there are several items that illustrate
the same subject, only a few examples are identified.
FIGURES
Figure 2 1. An electron microscope photograph of
MARV made from an ultrathin section
(magnification 87,500) 17
Figure 2 2. An electron microscope picture of
MARV particles negatively stained by uranyl
acetate (magnification 120,000) 17
Figure 3 1. Various MARV structures are visible at
different magnifications in these three
photos made of ultrathin sections 20,21
Figure 3 2. Various shapes of MARV virions are
apparent in electron microscope pictures
of negatively stained preparations
(magnification 120,000) 22
Figure 3 3. Various filoviral shapes negatively stained
by uranyl acetate (magnification 19,000) . 23
Figure 3 4. Schematic of a filovirus (adapted from
Feldmann, 1999) 25
Figure 3 5. Virus entry into the cell by receptor mediated
endocytosis and by direct fusion with the
plasma membrane 28
Figure 3 6. Patterns of receptor mediated endocytosis of
MARV; association of the virus with
clathrin coated pits 30
Figure 3 7. The first evidence of MARV infection is
the accumulation of viral material,
which sometimes appears as a
sponge like material 34, 35
Figure 3 8. Various sections showing the formation
and appearance of MARV nucleocapsids . 36
Figure 3 9. Schematic of formation of
MARV inclusions 37
Figure 3 10. MARV inclusions in Vero cells 38, 39
Figure 3 11. Nucleocapsids of ZEBOV in longitudinal
and cross sectional views 40
Figure 3 12. ZEBOV inclusion composed of helices 41
Figure 3 13. MARV budding (magnification 116,000) 43
Figure 3 14. Different patterns of the beginning stages
of MARV budding 44, 45
Figure 3 15. Variations in budding that lead to
ring shaped virions for MARV 45
Figure 3 16. A freeze etching picture of a MARV suspension
clearly showing rod and disc shaped virions
(magnification 80,000) 46
Figure 3 17. Schematic of the viral budding process
for filoviruses 47
Figure 3 18. ZEBOV infected cell of chick embryo
(magnification 10,700) 48
Figure 3 19. The development of net like structures in
filovirus infected cells 51, 52
Figure 4 1. MARV replication in a peritoneal macrophage
of a guinea pig as seen in an ultrathin
section of the cells of peritoneal exudate
obtained at 72 h post inoculation
(magnification 31,000) 65
Figure 4 2. An ultrathin section of a Kuppfer (macrophage)
cell in a liver of green monkey infected by
ZEBOV, day 3 post inoculation
(magnification 20,000) 69
Figure 4 3. Ultrathin sections showing replication
of filoviruses in hepatocytes of
green monkeys 72
Figure 4 4. Ultrathin section of adrenal cortex showing
replication of ZEBOV in green monkey
adrenal cortical cells (magnification 7,000) . 73
Figure 4 5. Ultrathin sections showing replication
of filoviruses in green monkey
endothelial cells 74, 75
Figure 4 6. Ultrathin sections showing replication
of ZEBOV in fibroblasts of rhesus
monkey 76, 77
Figure 4 7. ZEBOV replication occurs rarely in the
airway cells of green monkeys IS, 79
Figure 4 8. Sequential steps of filoviral dissemination
during infections in guinea pigs
and monkeys 83
Figure 5 1. An ultrathin section of macrophage
infected with ZEBOV in a green monkey's
lymphatic node (magnification 12,000) 89
Figure 5 2. Ultrathin section of a liver of guinea pig
sampled 7 days after inoculation with
MARV (magnification 11,000) 90
Figure 5 3. Light and electron microscope views of
ZEBOV infected liver in a baboon 91
Figure 5 4. The changes in liver pathology for ZEBOV
and MARV infections 92, 93
Figure 5 5. The ultrathin section of the liver of an
infected guinea pig with the replication
of MARV in the endothelial cell
(magnification 11,300) 94
Figure 5 6. Ultrathin sections highlighting differences
from the normal replication of ZEBOV
in the hepatocyte of a rhesus monkey 96
Figure 5 7. An ultrathin section of a green monkey's
lung showing replication of ZEBOV
in a circulating macrophage
(magnification 17,000) 99
Figure 5 8. An ultrathin section of the heart of a
green monkey infected with ZEBOV shows
replication in a macrophage located in
interstitial tissue (magnification 12,500) . 101
Figure 5 9. The schematic depicts the progression of
ZEBOV and MARV infection of tissues
and organs in fatally infected monkeys
and guinea pigs 102
Figure 6 1. Light microscope photographs showing
changes in the blood circulation in the liver,
kidney, and lung tissues in ZEBOV infected
monkeys 109
Figure 6 2. Two different views of microcirculation
damage in tissues 110, 111
Figure 6 3. An ultrathin section highlighting the
accumulation of neutrophils in an
alveolar capillary in a ZEBOV infected
green monkey (magnification 7,000) 112
Figure 6 4. A small arteriole in the spleen of a rhesus
monkey infected with ZEBOV
(magnification 10,000) 113
Figure 6 5. Epithelial cells in intestines are not
infected even at the terminal stages
of filoviral disease 114
Figure 6 6. The relation of fibrin clots to the surface of a
macrophage in a green monkey liver 116
Figure 6 7. Light and electron microscope pictures of
ZEBOV infected baboon adrenal gland tissue
that demonstrates prominent hemorrhages . . 117
Figure 6 8. An ultrathin section of a baboon spleen
showing diapedesis of erythrocytes into
interstitial tissue (magnification 14,000) . 121
Figure 6 9. The bone marrow tissue from a MARV
infected guinea pig 127
Figure 6 10. Events leading to blood system damage
in ZEBOV and MARV infected animals . 130
Figure 7 1. A section of the liver of guinea pig
infected with MARV before death
(magnification 6,000) 133
Figure 7 2. Fibrin thrombus clogging a hepatic sinusoid
in a rhesus monkey infected with ZEBOV
(magnification 10,500) 135
Figure 7 3. The effects of hemorrhages in the liver
of baboon infected with ZEBOV
(magnification 7,000) 136
Figure 7 4. Image from a light microscope showing the
liver from a guinea pig infected with
unadapted ZEBOV 138
Figure 7 5. The close contact of leukocytes with infected
hepatocytes from the liver of a guinea
pig infected by aerosolized MARV
(magnification 6,200) 139
Figure 7 6. The thrombus with various blood cells
in a splenic red pulp vessel
(magnification 8,000) 141
Figure 7 7. A ZEBOV infected macrophage in the
splenic red pulp of a green monkey
(magnification 16,000) 142
Figure 7 8. A fibrin thrombus in the lumen of a blood
vessel in the red pulp of a ZEBOV infected
green monkey (magnification 6,000) 143
Figure 7 9. Part of a green monkey's splenic red pulp
illustrating the main features of spleen
damage in the terminal stages of ZEBOV
infection (magnification 3,500) 144
Figure 7 10. Renal damage in green monkeys infected
with MARV via a semithin light microscope
section and an ultrathin electron
microscope section 147, 148
Figure 7 11. Two variations of the type of localized
renal tissue lesions that are seen in
filovirus infected animals 149, 150
Figure 7 12. Damage to the air blood barrier in a
ZEBOV infected green monkey
(magnification 4,000) 152
Figure 7 13. ZEBOV reproduction in the adrenal
glands of a green monkey 154, 155
Figure 7 14. A section from the heart of green
monkey infected with ZEBOV
(magnification 21,000) 157
Figure 8 1. A ZEBOV infected macrophage in the
lymphatic node of green monkey with
all the hallmarks of a filoviral infection
(magnification 16,000) 162
Figure 8 2. Two light microscope photographs of the
cortical portion of the inguinal lymphatic
nodes of a green monkey 164, 165
Figure 8 3. Light microscope photographs of a semithin
section of the cortical zone of a lymphatic
node from an infected monkey 166
Figure 8 4. Apoptosis of lymphocytes and plasma cells
in an infected green monkey's lymphatic
node, sampled before death
(magnification 8,500) 167
Figure 8 5. Swollen stromal (reticular) cell from a
MARV infected green monkey
(magnification 7,500) 169
Figure 8 6. Viral particles near the protrusions of
dendritic cell (magnification 27,700) 171
Figure 8 7. Light microscope photograph of severely
altered lymphatic node of baboon infected
with ZEBOV showing damage of
lymphatic tissue and hemorrhages 172
TABLES
Table 2 1. Description of the filoviral experiments in
animals that provided the samples for
microscopic studies 13
Table 3 1. Proposed functions of the filoviral proteins . 25
Table 3 2. Summary of differences in morphological
characteristics for MARV (strain Popp) and
ZEBOV infections 55
Table 4 1. The relation between dose and inoculation
mode on the time of filoviral detection
in blood (viremia) 62
Table 4 2. MARV titers (PFU) in peritoneal macrophage
cultured medium from various animals 67
Table 4 3. MARV titers (PFU) in peritoneal macrophage
cultured medium from baboons 67
Table 5 1. Summary of experimental design 87
Table 6 1. Species specific characteristics of
ZEBOV infected monkeys 119 |
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| id | DE-604.BV021572180 |
| illustrated | Illustrated |
| index_date | 2024-07-02T14:38:47Z |
| indexdate | 2024-07-09T20:38:56Z |
| institution | BVB |
| isbn | 0954523237 |
| language | English |
| lccn | 2004351692 |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-014787968 |
| oclc_num | 53191587 |
| open_access_boolean | |
| owner | DE-355 DE-BY-UBR DE-29 |
| owner_facet | DE-355 DE-BY-UBR DE-29 |
| physical | X, 369 S. Ill., graph. Darst. |
| publishDate | 2004 |
| publishDateSearch | 2004 |
| publishDateSort | 2004 |
| publisher | Horizon Bioscience |
| record_format | marc |
| spelling | Ebola and Marburg viruses molecular and cellular biology ed. by: Hans-Dieter Klenk ... Wymondham Horizon Bioscience 2004 X, 369 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Ebolavirus gtt Marburgvirus gtt Marburg virus Marburg virus disease Ebola virus disease Molecular virology Ebola-Virus (DE-588)4233551-6 gnd rswk-swf Ebola-Virus (DE-588)4233551-6 s b DE-604 Klenk, Hans-Dieter 1938-2021 Sonstige (DE-588)136121721 oth http://www.loc.gov/catdir/toc/fy053/2004351692.html Table of contents HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014787968&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Ebola and Marburg viruses molecular and cellular biology Ebolavirus gtt Marburgvirus gtt Marburg virus Marburg virus disease Ebola virus disease Molecular virology Ebola-Virus (DE-588)4233551-6 gnd |
| subject_GND | (DE-588)4233551-6 |
| title | Ebola and Marburg viruses molecular and cellular biology |
| title_auth | Ebola and Marburg viruses molecular and cellular biology |
| title_exact_search | Ebola and Marburg viruses molecular and cellular biology |
| title_exact_search_txtP | Ebola and Marburg viruses molecular and cellular biology |
| title_full | Ebola and Marburg viruses molecular and cellular biology ed. by: Hans-Dieter Klenk ... |
| title_fullStr | Ebola and Marburg viruses molecular and cellular biology ed. by: Hans-Dieter Klenk ... |
| title_full_unstemmed | Ebola and Marburg viruses molecular and cellular biology ed. by: Hans-Dieter Klenk ... |
| title_short | Ebola and Marburg viruses |
| title_sort | ebola and marburg viruses molecular and cellular biology |
| title_sub | molecular and cellular biology |
| topic | Ebolavirus gtt Marburgvirus gtt Marburg virus Marburg virus disease Ebola virus disease Molecular virology Ebola-Virus (DE-588)4233551-6 gnd |
| topic_facet | Ebolavirus Marburgvirus Marburg virus Marburg virus disease Ebola virus disease Molecular virology Ebola-Virus |
| url | http://www.loc.gov/catdir/toc/fy053/2004351692.html http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014787968&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT klenkhansdieter ebolaandmarburgvirusesmolecularandcellularbiology |