A new Cre-loxP based selection system for loss of heterozygosity:
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
2005
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Ulm, Univ., Diss., 2005 |
| Beschreibung: | 82 Bl. Ill., graph. Darst. 30 cm |
Internformat
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Datensatz im Suchindex
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| adam_text | Contents
Abbreviations 1
Chapter 1 Introduction 3
Chapter 2 Materials and Methods 9
2.1 Materials 9
2.2 Methods 16
2.2.1 Plasmid constructions 16
2.2.2 Methods in Molecular Biology 18
2.2.3 Work with prokaryotic cells 24
2.2.4 Plasmid rescue 26
2.2.5 Work with eukaryotic cells 27
2.2.6 Bystander effect 34
Chapter 3 Results 35
3.1 A new Cre loxP based strategy to select for
homozygosity 35
3.2 Cre mediated recombination between mutant
loxP Sites 37
3.2.1 Construction of the GFP IRES TK CD14 loxP
recombination cassette 37
3.2.2 Testing of the mutant loxP Sites in E. coli 37
3.2.3 Testing of the recombination cassette in mammalian
cell lines 39
3.3 Selection for LOH 42
3.3.2 Experimental design 42
3.3.3 Plasmid rescue 43
3.3.4 Testing of the selection System in
70Z/3 neo ires tk hygro 47
3.4 Comparison of bystander effect after treatment
with GCV or BVDU 52
Chapter 4 Discussion 54
4.1 Selection for LOH in a murine B lymphoid cell line 54
4.2 Weak points of the selection System 56
4.3 BVDU: a better prodrug for the TK selection
System in vitro 57
4.4 Applications of the Cre loxP based selection
technique 58
4.4.1 Mammalian genetics in the study of gene function 58
4.4.2 Retroviral insertional mutagenesis 59
4.4.3 Homozygous mutant embryonic stem cells 63
4.5 Outview 65
Chapter 5 Summary 66
Chapter 6 References 68
Acknowledgements
|
| adam_txt |
Contents
Abbreviations 1
Chapter 1 Introduction 3
Chapter 2 Materials and Methods 9
2.1 Materials 9
2.2 Methods 16
2.2.1 Plasmid constructions 16
2.2.2 Methods in Molecular Biology 18
2.2.3 Work with prokaryotic cells 24
2.2.4 Plasmid rescue 26
2.2.5 Work with eukaryotic cells 27
2.2.6 Bystander effect 34
Chapter 3 Results 35
3.1 A new Cre loxP based strategy to select for
homozygosity 35
3.2 Cre mediated recombination between mutant
loxP Sites 37
3.2.1 Construction of the GFP IRES TK CD14 loxP
recombination cassette 37
3.2.2 Testing of the mutant loxP Sites in E. coli 37
3.2.3 Testing of the recombination cassette in mammalian
cell lines 39
3.3 Selection for LOH 42
3.3.2 Experimental design 42
3.3.3 Plasmid rescue 43
3.3.4 Testing of the selection System in
70Z/3 neo ires tk hygro 47
3.4 Comparison of bystander effect after treatment
with GCV or BVDU 52
Chapter 4 Discussion 54
4.1 Selection for LOH in a murine B lymphoid cell line 54
4.2 Weak points of the selection System 56
4.3 BVDU: a better prodrug for the TK selection
System in vitro 57
4.4 Applications of the Cre loxP based selection
technique 58
4.4.1 Mammalian genetics in the study of gene function 58
4.4.2 Retroviral insertional mutagenesis 59
4.4.3 Homozygous mutant embryonic stem cells 63
4.5 Outview 65
Chapter 5 Summary 66
Chapter 6 References 68
Acknowledgements |
| any_adam_object | 1 |
| any_adam_object_boolean | 1 |
| author | Behlau, Carla |
| author_facet | Behlau, Carla |
| author_role | aut |
| author_sort | Behlau, Carla |
| author_variant | c b cb |
| building | Verbundindex |
| bvnumber | BV021519314 |
| ctrlnum | (OCoLC)255243276 (DE-599)BVBBV021519314 |
| dewey-full | 612.01 |
| dewey-hundreds | 600 - Technology (Applied sciences) |
| dewey-ones | 612 - Human physiology |
| dewey-raw | 612.01 |
| dewey-search | 612.01 |
| dewey-sort | 3612.01 |
| dewey-tens | 610 - Medicine and health |
| discipline | Medizin |
| discipline_str_mv | Medizin |
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| illustrated | Illustrated |
| index_date | 2024-07-02T14:21:59Z |
| indexdate | 2024-07-09T20:37:40Z |
| institution | BVB |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-014735836 |
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| physical | 82 Bl. Ill., graph. Darst. 30 cm |
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| spelling | Behlau, Carla Verfasser aut A new Cre-loxP based selection system for loss of heterozygosity von Carla Behlau 2005 82 Bl. Ill., graph. Darst. 30 cm txt rdacontent n rdamedia nc rdacarrier Ulm, Univ., Diss., 2005 (DE-588)4113937-9 Hochschulschrift gnd-content HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014735836&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Behlau, Carla A new Cre-loxP based selection system for loss of heterozygosity |
| subject_GND | (DE-588)4113937-9 |
| title | A new Cre-loxP based selection system for loss of heterozygosity |
| title_auth | A new Cre-loxP based selection system for loss of heterozygosity |
| title_exact_search | A new Cre-loxP based selection system for loss of heterozygosity |
| title_exact_search_txtP | A new Cre-loxP based selection system for loss of heterozygosity |
| title_full | A new Cre-loxP based selection system for loss of heterozygosity von Carla Behlau |
| title_fullStr | A new Cre-loxP based selection system for loss of heterozygosity von Carla Behlau |
| title_full_unstemmed | A new Cre-loxP based selection system for loss of heterozygosity von Carla Behlau |
| title_short | A new Cre-loxP based selection system for loss of heterozygosity |
| title_sort | a new cre loxp based selection system for loss of heterozygosity |
| topic_facet | Hochschulschrift |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014735836&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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