The application of the herpes simplex virus protein VP22 as a new tool for a peptide mediated therapy for pancreatic carcinoma:
Gespeichert in:
| 1. Verfasser: | |
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| Format: | Mikrofilm Buch |
| Sprache: | English |
| Veröffentlicht: |
2005
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| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Kiel, Univ., Diss., 2005 |
| Beschreibung: | 46 Bl. Ill., graph. Darst |
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Datensatz im Suchindex
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| adam_text | TABLE OF CONTENTS
1. INTRODUCTION 1
1.lPancreatic carcinoma and therapeutic strategies 1
1.2 Cancer gene therapy 2
1.2.1 The approaches of cancer gene therapy 2
1.2.2 The delivery vehicle of cancer gene therapy 2
1.2.3 Reporter genes and gene therapy 3
1.3 Green fluorescence protein (GFP) 4
1.3.1 GFP as a fusion tag 4
1.4 The herpes simplex virus type 1 virus protein VP22 5
1.4.1 The biological, biochemical and immunological properties of VP22 5
1.4.2 The mechanism of VP22 trafficking 5
1.4.3 VP22 in gene therapy 6
2.5 Research aims 7
2. MATERIALS AND METHODS 8
2.1 MATERIALS 8
2.1.1 Bacteriological reagents 8
2.1.2 Cell culture medium and components 8
2.1.3 Immunological reagents 8
2.1.4 Molecular biological reagents 9
2.1.5 Staining reagents 9
2.1.6 Protein purification 9
2.1.7 PCR 10
2.1.8 Plasmid 10
2.1.9 Bacteria 10
2.1.10 Cell lines 10
2.2 METHODS 11
2.2.1 Plasmid construction 12
2.2.2 Cell culture 13
2.2.3 Transfection 14
2.2.4 Protein production 14
2.2.5 Application of Purified Protein in Mammalian Cells 15
2.2.6 Immunofluorescence 15
2.2.7 Fluorescence-activated cell sorting (FACS) 15
2.2.8 Western blot analysis 15
3. RESULTS 16
3.1 Construction of pVP22EGFP eukaryontic expression vector 16
3.2 Intercellular transport of the VP22-EGFP fusion protein 16
3.3 VP22EGFP fusion protein delivery between different cell lines 17
3.4 Construction of pT7VP22EGFP prokaryontic expression vector 17
3.5 Characterization of the purified VP22EGFP fusion protein 18
3.6 VP22EGFP fusion protein transduction of human mammalian cells 20
3.6.1 Protein transduction of 293T cells 20
3.6.2 VP22EGFP protein transduction in human pancreatic carcinoma cell
lines 22
3.6.2.1 Quantitative uptake of VP22EGFP fusion protein in Panc89 cells 22
3.6.2.2 Stability of VP22EGFP fusion protein expression in Panc89 cells 24
3.6.3 VP22EGFP fusion protein in the three dimensional pancreatic
carcinoma cell culture model 26
4. DISCUSSION 27
5. SUMMARY 32
6. REFFERENCES 33
7. ENCLOSURE 39
8. ACKNOWLEDGMENTS 45
9. CURRICULUM VITAE 46
|
| adam_txt |
TABLE OF CONTENTS
1. INTRODUCTION 1
1.lPancreatic carcinoma and therapeutic strategies 1
1.2 Cancer gene therapy 2
1.2.1 The approaches of cancer gene therapy 2
1.2.2 The delivery vehicle of cancer gene therapy 2
1.2.3 Reporter genes and gene therapy 3
1.3 Green fluorescence protein (GFP) 4
1.3.1 GFP as a fusion tag 4
1.4 The herpes simplex virus type 1 virus protein VP22 5
1.4.1 The biological, biochemical and immunological properties of VP22 5
1.4.2 The mechanism of VP22 trafficking 5
1.4.3 VP22 in gene therapy 6
2.5 Research aims 7
2. MATERIALS AND METHODS 8
2.1 MATERIALS 8
2.1.1 Bacteriological reagents 8
2.1.2 Cell culture medium and components 8
2.1.3 Immunological reagents 8
2.1.4 Molecular biological reagents 9
2.1.5 Staining reagents 9
2.1.6 Protein purification 9
2.1.7 PCR 10
2.1.8 Plasmid 10
2.1.9 Bacteria 10
2.1.10 Cell lines 10
2.2 METHODS 11
2.2.1 Plasmid construction 12
2.2.2 Cell culture 13
2.2.3 Transfection 14
2.2.4 Protein production 14
2.2.5 Application of Purified Protein in Mammalian Cells 15
2.2.6 Immunofluorescence 15
2.2.7 Fluorescence-activated cell sorting (FACS) 15
2.2.8 Western blot analysis 15
3. RESULTS 16
3.1 Construction of pVP22EGFP eukaryontic expression vector 16
3.2 Intercellular transport of the VP22-EGFP fusion protein 16
3.3 VP22EGFP fusion protein delivery between different cell lines 17
3.4 Construction of pT7VP22EGFP prokaryontic expression vector 17
3.5 Characterization of the purified VP22EGFP fusion protein 18
3.6 VP22EGFP fusion protein transduction of human mammalian cells 20
3.6.1 Protein transduction of 293T cells 20
3.6.2 VP22EGFP protein transduction in human pancreatic carcinoma cell
lines 22
3.6.2.1 Quantitative uptake of VP22EGFP fusion protein in Panc89 cells 22
3.6.2.2 Stability of VP22EGFP fusion protein expression in Panc89 cells 24
3.6.3 VP22EGFP fusion protein in the three dimensional pancreatic
carcinoma cell culture model 26
4. DISCUSSION 27
5. SUMMARY 32
6. REFFERENCES 33
7. ENCLOSURE 39
8. ACKNOWLEDGMENTS 45
9. CURRICULUM VITAE 46 |
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| index_date | 2024-07-02T14:20:37Z |
| indexdate | 2024-07-09T20:37:35Z |
| institution | BVB |
| language | English |
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| spelling | Chu, Kang 1969- Verfasser (DE-588)130097004 aut The application of the herpes simplex virus protein VP22 as a new tool for a peptide mediated therapy for pancreatic carcinoma vorgelegt von Kang Chu 2005 46 Bl. Ill., graph. Darst txt rdacontent h rdamedia he rdacarrier Kiel, Univ., Diss., 2005 Mikroform-Ausgabe 2005 1 Mikrofiche : 24x Mikrofiche-Ausg.: [Mikrofiche-Ausg.] Signatur des reproduzierten Kulturobjekts: DBL_x001C_2005 MF 757 s2005 (DE-588)4113937-9 Hochschulschrift gnd-content Reproduktion von Chu, Kang, 1969- The application of the herpes simplex virus protein VP22 as a new tool for a peptide mediated therapy for pancreatic carcinoma 2005 HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014732241&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Chu, Kang 1969- The application of the herpes simplex virus protein VP22 as a new tool for a peptide mediated therapy for pancreatic carcinoma |
| subject_GND | (DE-588)4113937-9 |
| title | The application of the herpes simplex virus protein VP22 as a new tool for a peptide mediated therapy for pancreatic carcinoma |
| title_auth | The application of the herpes simplex virus protein VP22 as a new tool for a peptide mediated therapy for pancreatic carcinoma |
| title_exact_search | The application of the herpes simplex virus protein VP22 as a new tool for a peptide mediated therapy for pancreatic carcinoma |
| title_exact_search_txtP | The application of the herpes simplex virus protein VP22 as a new tool for a peptide mediated therapy for pancreatic carcinoma |
| title_full | The application of the herpes simplex virus protein VP22 as a new tool for a peptide mediated therapy for pancreatic carcinoma vorgelegt von Kang Chu |
| title_fullStr | The application of the herpes simplex virus protein VP22 as a new tool for a peptide mediated therapy for pancreatic carcinoma vorgelegt von Kang Chu |
| title_full_unstemmed | The application of the herpes simplex virus protein VP22 as a new tool for a peptide mediated therapy for pancreatic carcinoma vorgelegt von Kang Chu |
| title_short | The application of the herpes simplex virus protein VP22 as a new tool for a peptide mediated therapy for pancreatic carcinoma |
| title_sort | the application of the herpes simplex virus protein vp22 as a new tool for a peptide mediated therapy for pancreatic carcinoma |
| topic_facet | Hochschulschrift |
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