Techniques for molecular biology:
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English French |
| Veröffentlicht: |
Enfield (NH) [u.a.]
Science Publishers
2006
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | Translation of: Principes des techniques de biologie moléculaire. 2e ed. rev. et augm. Paris : INRA, 2003. Includes bibliographical references and index |
| Beschreibung: | VII, 227 S. graph. Darst. |
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| 245 | 1 | 0 | |a Techniques for molecular biology |c editors, D. Tagu and C. Moussard |
| 264 | 1 | |a Enfield (NH) [u.a.] |b Science Publishers |c 2006 | |
| 300 | |a VII, 227 S. |b graph. Darst. | ||
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| 500 | |a Translation of: Principes des techniques de biologie moléculaire. 2e ed. rev. et augm. Paris : INRA, 2003. | ||
| 500 | |a Includes bibliographical references and index | ||
| 650 | 7 | |a Biologia molecular (técnicas) |2 larpcal | |
| 650 | 4 | |a Molecular biology |x Technique | |
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Datensatz im Suchindex
| _version_ | 1804135133180067840 |
|---|---|
| adam_text | CONTENTS
I. Definition 1
1. Structure and Expression of a Eukaryote Gene Coding for an
mRNA and a Protein 3
2. Parameters of Description of a Genome 5
3. Sequencing of Whole Genomes 9
II. Vectors and Cloning 13
4. Restriction Enzymes 15
5. Electrophoresis of Nucleic Acids 19
6. Description of a Plasmid and a Phagemid 21
7. Description of a Bacteriophage and a Cosmid 23
8. Description of a YAC and Other Large Vectors 25
9. Molecular Cloning 27
10. Genetic Transformation of Bacteria and Yeasts 31
III. Labelling of Nucleic Acids and Hybridization 33
11. DNA Labelling 35
12. Molecular Hybridization 39
13. In situ Hybridization of mRNA 43
IV. DNA Libraries and Screening 47
14. Construction of a Genomic DNA Library 49
15. Construction of a cDNA Library 51
16. Screening of a Library 53
17. Differential Screening: Subtractive Libraries, AFLP cDNA 57
18. Differential Display RT PCR 61
19. Differential Screening by SSH: Suppression Subtractive 65
Hybridization
20. Differential Screening by RDA: Representational
Difference Analysis 69
21. EST: Expressed Sequence Tags 73
22. DNA Microarrays: DNA Microchips, cDNA Filters 77
V. Characterization of a Gene 89
23. DNA Sequencing 91
24. PCR: Polymerase Chain Reaction 97
25. RACE: Rapid Amplification of cDNA Ends 99
26. Genome Walking by PCR 103
27. RT PCR: Reverse Transcriptase PCR 105
28. In vitro Transcription 109
29. Determination of Transcription Initiation Site 111
30. Functional Analysis of Promoters 113
31. Gel Retardation 117
32. DNase I Footprinting 119
VI. Genetic Transformation of Eukaryotes 121
33. Genetic Transformation of Plants by Agrobacterium tumefaciens 123
34. Direct Gene Transfer into Plant Protoplasts 129
35. Direct Gene Transfer by Biolistics 131
36. Genetic Transformation of Animal Cells 133
37. Cloning of Animals 137
38. Transient Expression 141
VII. Analysis of Gene Function 143
39. Recombinant Proteins 145
40. Baculoviruses of Insects, Vectors of Expression of Foreign Genes 149
41. Yeast two Hybrid System 155
42. Site Directed Mutagenesis 161
43. Mutation Complementation in Yeast 167
44. Knock out of Genes in Yeast 171
45. Gene Tagging 175
46. RNA Interference 181
VIII. Polymorphism of a Genome 185 *
47. Molecular Markers 187
48. Genetic and Physical Maps 193
49. PFGE: Pulse Field Gel Electrophoresis 197
50. RFLP: Restriction Fragment Length Polymorphism 201
51. RAPD: Random Amplified Polymorphic DNA 203
52. AFLP: Amplified Fragment Length Polymorphism 205
53. Retromarkers 207
54. SSCP: Single Strand Conformation Polymorphism 213
Contents
vi
55. DGGE: Denaturing Gel Gradient Electrophoresis 215
56. SNP: Single Nucleotide Polymorphism 217
57. Simple Sequence Repeats (SSR): Microsatellites 219
List of Contributors 225
Contents
vii
|
| adam_txt |
CONTENTS
I. Definition 1
1. Structure and Expression of a Eukaryote Gene Coding for an
mRNA and a Protein 3
2. Parameters of Description of a Genome 5
3. Sequencing of Whole Genomes 9
II. Vectors and Cloning 13
4. Restriction Enzymes 15
5. Electrophoresis of Nucleic Acids 19
6. Description of a Plasmid and a Phagemid 21
7. Description of a Bacteriophage and a Cosmid 23
8. Description of a YAC and Other Large Vectors 25
9. Molecular Cloning 27
10. Genetic Transformation of Bacteria and Yeasts 31
III. Labelling of Nucleic Acids and Hybridization 33
11. DNA Labelling 35
12. Molecular Hybridization 39
13. In situ Hybridization of mRNA 43
IV. DNA Libraries and Screening 47
14. Construction of a Genomic DNA Library 49
15. Construction of a cDNA Library 51
16. Screening of a Library 53
17. Differential Screening: Subtractive Libraries, AFLP cDNA 57
18. Differential Display RT PCR 61
19. Differential Screening by SSH: Suppression Subtractive 65
Hybridization
20. Differential Screening by RDA: Representational
Difference Analysis 69
21. EST: Expressed Sequence Tags 73
22. DNA Microarrays: DNA Microchips, cDNA Filters 77
V. Characterization of a Gene 89
23. DNA Sequencing 91
24. PCR: Polymerase Chain Reaction 97
25. RACE: Rapid Amplification of cDNA Ends 99
26. Genome Walking by PCR 103
27. RT PCR: Reverse Transcriptase PCR 105
28. In vitro Transcription 109
29. Determination of Transcription Initiation Site 111
30. Functional Analysis of Promoters 113
31. Gel Retardation 117
32. DNase I Footprinting 119
VI. Genetic Transformation of Eukaryotes 121
33. Genetic Transformation of Plants by Agrobacterium tumefaciens 123
34. Direct Gene Transfer into Plant Protoplasts 129
35. Direct Gene Transfer by Biolistics 131
36. Genetic Transformation of Animal Cells 133
37. Cloning of Animals 137
38. Transient Expression 141
VII. Analysis of Gene Function 143
39. Recombinant Proteins 145
40. Baculoviruses of Insects, Vectors of Expression of Foreign Genes 149
41. Yeast two Hybrid System 155
42. Site Directed Mutagenesis 161
43. Mutation Complementation in Yeast 167
44. Knock out of Genes in Yeast 171
45. Gene Tagging 175
46. RNA Interference 181
VIII. Polymorphism of a Genome 185 *
47. Molecular Markers 187
48. Genetic and Physical Maps 193
49. PFGE: Pulse Field Gel Electrophoresis 197
50. RFLP: Restriction Fragment Length Polymorphism 201
51. RAPD: Random Amplified Polymorphic DNA 203
52. AFLP: Amplified Fragment Length Polymorphism 205
53. Retromarkers 207
54. SSCP: Single Strand Conformation Polymorphism 213
Contents
vi
55. DGGE: Denaturing Gel Gradient Electrophoresis 215
56. SNP: Single Nucleotide Polymorphism 217
57. Simple Sequence Repeats (SSR): Microsatellites 219
List of Contributors 225
Contents
vii |
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| index_date | 2024-07-02T13:59:34Z |
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| language | English French |
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| spelling | Principes des techniques de biologie moléculaire Techniques for molecular biology editors, D. Tagu and C. Moussard Enfield (NH) [u.a.] Science Publishers 2006 VII, 227 S. graph. Darst. txt rdacontent n rdamedia nc rdacarrier Translation of: Principes des techniques de biologie moléculaire. 2e ed. rev. et augm. Paris : INRA, 2003. Includes bibliographical references and index Biologia molecular (técnicas) larpcal Molecular biology Technique Molecular genetics Technique Plant molecular biology Technique Methode (DE-588)4038971-6 gnd rswk-swf Molekulargenetik (DE-588)4039987-4 gnd rswk-swf Mikrobiologe (DE-588)4039199-1 gnd rswk-swf Genanalyse (DE-588)4200230-8 gnd rswk-swf Mikrobiologische Analyse (DE-588)4418240-5 gnd rswk-swf Mikrobiologe (DE-588)4039199-1 s Methode (DE-588)4038971-6 s DE-604 Mikrobiologische Analyse (DE-588)4418240-5 s Molekulargenetik (DE-588)4039987-4 s Genanalyse (DE-588)4200230-8 s b DE-604 Tagu, Denis Sonstige oth HBZ Datenaustausch application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014643740&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Techniques for molecular biology Biologia molecular (técnicas) larpcal Molecular biology Technique Molecular genetics Technique Plant molecular biology Technique Methode (DE-588)4038971-6 gnd Molekulargenetik (DE-588)4039987-4 gnd Mikrobiologe (DE-588)4039199-1 gnd Genanalyse (DE-588)4200230-8 gnd Mikrobiologische Analyse (DE-588)4418240-5 gnd |
| subject_GND | (DE-588)4038971-6 (DE-588)4039987-4 (DE-588)4039199-1 (DE-588)4200230-8 (DE-588)4418240-5 |
| title | Techniques for molecular biology |
| title_alt | Principes des techniques de biologie moléculaire |
| title_auth | Techniques for molecular biology |
| title_exact_search | Techniques for molecular biology |
| title_exact_search_txtP | Techniques for molecular biology |
| title_full | Techniques for molecular biology editors, D. Tagu and C. Moussard |
| title_fullStr | Techniques for molecular biology editors, D. Tagu and C. Moussard |
| title_full_unstemmed | Techniques for molecular biology editors, D. Tagu and C. Moussard |
| title_short | Techniques for molecular biology |
| title_sort | techniques for molecular biology |
| topic | Biologia molecular (técnicas) larpcal Molecular biology Technique Molecular genetics Technique Plant molecular biology Technique Methode (DE-588)4038971-6 gnd Molekulargenetik (DE-588)4039987-4 gnd Mikrobiologe (DE-588)4039199-1 gnd Genanalyse (DE-588)4200230-8 gnd Mikrobiologische Analyse (DE-588)4418240-5 gnd |
| topic_facet | Biologia molecular (técnicas) Molecular biology Technique Molecular genetics Technique Plant molecular biology Technique Methode Molekulargenetik Mikrobiologe Genanalyse Mikrobiologische Analyse |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014643740&sequence=000002&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
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