PCR:
Gespeichert in:
| Hauptverfasser: | , |
|---|---|
| Format: | Buch |
| Sprache: | English |
| Veröffentlicht: |
New York, NY [u.a.]
Taylor & Francis
2006
|
| Ausgabe: | 2. ed. |
| Schriftenreihe: | The basics
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | X, 292 S. Ill., graph. Darst. |
| ISBN: | 0415355478 |
Internformat
MARC
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| 100 | 1 | |a McPherson, Michael J. |d 1970- |e Verfasser |0 (DE-588)131817639 |4 aut | |
| 245 | 1 | 0 | |a PCR |c Michael J. McPherson and Simon Geir Møller |
| 250 | |a 2. ed. | ||
| 264 | 1 | |a New York, NY [u.a.] |b Taylor & Francis |c 2006 | |
| 300 | |a X, 292 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
| 337 | |b n |2 rdamedia | ||
| 338 | |b nc |2 rdacarrier | ||
| 490 | 0 | |a The basics | |
| 650 | 4 | |a Ligase Chain Reaction |x methods | |
| 650 | 4 | |a Polymerase Chain Reaction |x methods | |
| 650 | 4 | |a Polymerase chain reaction | |
| 650 | 0 | 7 | |a Labortechnik |0 (DE-588)4123602-6 |2 gnd |9 rswk-swf |
| 650 | 0 | 7 | |a Polymerase-Kettenreaktion |0 (DE-588)4256726-9 |2 gnd |9 rswk-swf |
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| 999 | |a oai:aleph.bib-bvb.de:BVB01-014195184 | ||
| 883 | 1 | |8 1\p |a cgwrk |d 20201028 |q DE-101 |u https://d-nb.info/provenance/plan#cgwrk | |
Datensatz im Suchindex
| _version_ | 1804134601130508288 |
|---|---|
| adam_text | Contents
Abbreviations
Preface
ix
xi
Chapter
1.1
1.2
1.3
1.4
1.5
1
1
1
3
5
6
Chapter
2.1
2.2
2.3
2.4
2.5
Protocol
9
9
11
15
18
18
20
Chapter
3.1
3.2
3.3
3.4
3.5
3.6
3.7
3.8 DNA
3.9
3.10
3.11
3.12
3.13
3.14
3.15
3.16
3.17
3.18
3.19
Protocol
vi
Chapter
4.1
4.2
4.3
4.4
4.5
4.6
4.7
65
65
65
75
76
76
80
82
Chapter
PCR products
Introduction
Analysis of PCR products
Verification of initial amplification product
Direct
Direct labeling of PCR products and homogenous assays
In vitro expression of PCR products
5.1
5.2
5.3
5.4
5.5
5.6
Protocol
terminators
87
87
87
89
93
101
103
108
Chapter
6.1
6.2
6.3
6.4
6.5
Protocol
Protocol
111
111
111
115
117
131
134
135
Chapter
Introduction
Inverse PCR mutagenesis
Unique sites elimination
Splicing by overlap extension (SOEing)
Point mutations
Deletions and insertions
Deletion mutagenesis
Insertion mutagenesis
Random mutagenesis
PCR misincorporation procedures
Recombination strategies
RACHITT
. .*„
Protocol
Protocol
Protocol
Protocol
7.1
7.2
7.3
7.4
7.5
7.6
7.7
7.8
7.9
7.10
7.11
7.12
7.13
137
137
138
144
144
150
151
151
151
157
159
160
166
166
171
173
175
177
Contents
Protocol
Protocol
179
182
Chapter
8.1
8.2
8.3
8.4
8.5
8.6
8.7
8.8
Protocol
185
185
185
189
194
194
198
204
205
208
Chapter
209
9.1
9.2
9.3
9.4
9.5
9.6
9.7
9.8
9.9
Chapter
Cloning genes of known
Using PCR to clone expressed genes
Express sequence tags
Rapid amplification of cDNA ends (RACE)
Isolation of unknown
Inverse polymerase chain reaction (IPCR)
Multiplex restriction site PCR (mrPCR)
Vectorette and splinkerette PCR
Degenerate primers based on peptide sequence
A
10.1
10.2
10.3
B
10.4
10.5
10.6
10.7
Protocol
Protocol
233
233
233
237
238
240
240
243
244
248
253
255
Chapter
11.1
11.2
11.3
11.4
(DHPLC)
11.5
11.6
257
257
258
259
263
264
264
viii Contents
11.7
11.8
11.9
11.10
11.11
11.12
11.13
11.14
11.15
11.16
Index
|
| adam_txt |
Contents
Abbreviations
Preface
ix
xi
Chapter
1.1
1.2
1.3
1.4
1.5
1
1
1
3
5
6
Chapter
2.1
2.2
2.3
2.4
2.5
Protocol
9
9
11
15
18
18
20
Chapter
3.1
3.2
3.3
3.4
3.5
3.6
3.7
3.8 DNA
3.9
3.10
3.11
3.12
3.13
3.14
3.15
3.16
3.17
3.18
3.19
Protocol
vi
Chapter
4.1
4.2
4.3
4.4
4.5
4.6
4.7
65
65
65
75
76
76
80
82
Chapter
PCR products
Introduction
Analysis of PCR products
Verification of initial amplification product
Direct
Direct labeling of PCR products and homogenous assays
In vitro expression of PCR products
5.1
5.2
5.3
5.4
5.5
5.6
Protocol
terminators
87
87
87
89
93
101
103
108
Chapter
6.1
6.2
6.3
6.4
6.5
Protocol
Protocol
111
111
111
115
117
131
134
135
Chapter
Introduction
Inverse PCR mutagenesis
Unique sites elimination
Splicing by overlap extension (SOEing)
Point mutations
Deletions and insertions
Deletion mutagenesis
Insertion mutagenesis
Random mutagenesis
PCR misincorporation procedures
Recombination strategies
RACHITT
. .*„
Protocol
Protocol
Protocol
Protocol
7.1
7.2
7.3
7.4
7.5
7.6
7.7
7.8
7.9
7.10
7.11
7.12
7.13
137
137
138
144
144
150
151
151
151
157
159
160
166
166
171
173
175
177
Contents
Protocol
Protocol
179
182
Chapter
8.1
8.2
8.3
8.4
8.5
8.6
8.7
8.8
Protocol
185
185
185
189
194
194
198
204
205
208
Chapter
209
9.1
9.2
9.3
9.4
9.5
9.6
9.7
9.8
9.9
Chapter
Cloning genes of known
Using PCR to clone expressed genes
Express sequence tags
Rapid amplification of cDNA ends (RACE)
Isolation of unknown
Inverse polymerase chain reaction (IPCR)
Multiplex restriction site PCR (mrPCR)
Vectorette and splinkerette PCR
Degenerate primers based on peptide sequence
A
10.1
10.2
10.3
B
10.4
10.5
10.6
10.7
Protocol
Protocol
233
233
233
237
238
240
240
243
244
248
253
255
Chapter
11.1
11.2
11.3
11.4
(DHPLC)
11.5
11.6
257
257
258
259
263
264
264
viii Contents
11.7
11.8
11.9
11.10
11.11
11.12
11.13
11.14
11.15
11.16
Index |
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| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 572 - Biochemistry |
| dewey-raw | 572.86 572.43 |
| dewey-search | 572.86 572.43 |
| dewey-sort | 3572.86 |
| dewey-tens | 570 - Biology |
| discipline | Biologie Chemie Chemie-Ingenieurwesen Biotechnologie Medizin |
| discipline_str_mv | Biologie Chemie Chemie-Ingenieurwesen Biotechnologie Medizin |
| edition | 2. ed. |
| format | Book |
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| index_date | 2024-07-02T13:26:46Z |
| indexdate | 2024-07-09T20:27:12Z |
| institution | BVB |
| isbn | 0415355478 |
| language | English |
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| series2 | The basics |
| spelling | McPherson, Michael J. 1970- Verfasser (DE-588)131817639 aut PCR Michael J. McPherson and Simon Geir Møller 2. ed. New York, NY [u.a.] Taylor & Francis 2006 X, 292 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier The basics Ligase Chain Reaction methods Polymerase Chain Reaction methods Polymerase chain reaction Labortechnik (DE-588)4123602-6 gnd rswk-swf Polymerase-Kettenreaktion (DE-588)4256726-9 gnd rswk-swf Polymerase-Kettenreaktion (DE-588)4256726-9 s Labortechnik (DE-588)4123602-6 s 1\p DE-604 Møller, Simon Geir Verfasser (DE-588)13181771X aut Digitalisierung UBRegensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014195184&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis 1\p cgwrk 20201028 DE-101 https://d-nb.info/provenance/plan#cgwrk |
| spellingShingle | McPherson, Michael J. 1970- Møller, Simon Geir PCR Ligase Chain Reaction methods Polymerase Chain Reaction methods Polymerase chain reaction Labortechnik (DE-588)4123602-6 gnd Polymerase-Kettenreaktion (DE-588)4256726-9 gnd |
| subject_GND | (DE-588)4123602-6 (DE-588)4256726-9 |
| title | PCR |
| title_auth | PCR |
| title_exact_search | PCR |
| title_exact_search_txtP | PCR |
| title_full | PCR Michael J. McPherson and Simon Geir Møller |
| title_fullStr | PCR Michael J. McPherson and Simon Geir Møller |
| title_full_unstemmed | PCR Michael J. McPherson and Simon Geir Møller |
| title_short | PCR |
| title_sort | pcr |
| topic | Ligase Chain Reaction methods Polymerase Chain Reaction methods Polymerase chain reaction Labortechnik (DE-588)4123602-6 gnd Polymerase-Kettenreaktion (DE-588)4256726-9 gnd |
| topic_facet | Ligase Chain Reaction methods Polymerase Chain Reaction methods Polymerase chain reaction Labortechnik Polymerase-Kettenreaktion |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014195184&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT mcphersonmichaelj pcr AT møllersimongeir pcr |