Enzyme assays: high-throughput screening, genetic selection and fingerprinting
Gespeichert in:
| Format: | Buch |
|---|---|
| Sprache: | English |
| Veröffentlicht: |
Weinheim
WILEY-VCH
2006
|
| Schlagworte: | |
| Online-Zugang: | Inhaltsverzeichnis |
| Beschreibung: | XVIII, 368 S. Ill., graph. Darst. |
| ISBN: | 3527310959 9783527310951 |
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| 245 | 1 | 0 | |a Enzyme assays |b high-throughput screening, genetic selection and fingerprinting |c ed. by Jean-Louis Reymond |
| 264 | 1 | |a Weinheim |b WILEY-VCH |c 2006 | |
| 300 | |a XVIII, 368 S. |b Ill., graph. Darst. | ||
| 336 | |b txt |2 rdacontent | ||
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| 650 | 4 | |a Enzym - Screening | |
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| 700 | 1 | |a Reymond, Jean-Louis |e Sonstige |0 (DE-588)130585467 |4 oth | |
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Datensatz im Suchindex
| _version_ | 1804134562994847744 |
|---|---|
| adam_text | Contents
Preface XIII
List of Contributors XV
Introduction
Renaud Sicard
Enzyme Assays
Part I: The Chemistry of Enzyme Assays
Part II: Enzyme Assays and Genetic Selection
Part III: Enzyme Profiling
Enzyme Assays in Other Areas
How to Use this Book
Part I High-throughput Screening
1
Using Color Changes
Romas J. Kazlauskas
1.1
1.2
1.3
1.3.1
1.3.2
1.3.2.1
(Testing Many Hydrolases Toward One Substrate)
1.3.2.2
(Testing Many Substrates Toward Hydrolase)
1.3.3
1.4
1.4.1
1.4.2
(Quick
Enzyme Assays: High-throughput Screening, Genetic Selection and Fingerprinting
Edited by Jean-Louis Reymond
Copyright
ISBN:
VI
1.4.2.1 Chromogenic Substrate 32
1.4.2.2 pH
1.4.3 Application 33
1.4.3.1 Substrate
1.4.3.2
IAA
References
2
the Enantioselectivity of Enzymes
Manfred T. Reetz
2.1
2.2
2.2.1
of Chiral p-Nitrophenyl Esters
2.2.2
2.2.3
(EMDee)
2.2.4
Enantiomeric Excess
2.2.5
2.3
2.3.1
2.3.2
2.3.3
2.4
2.4.1
2.5
2.6
for Assaying Lipases or
2.7
2.8
2.9
2.10
2.11
2.12
References
3
for Oxidoreductases
Tyler W. Johannes, Ryan D.
3.1
3.2
3.2.1
Contents
3.2.1.1
3.2.1.2
3.2.2
3.2.2.1
3.2.2.2
3.2.2.3
3.2.3
3.2.3.1
3.2.3.2
3.2.3.3
3.2.3.4
3.2.3.5
3.2.4
3.2.4.1 ABTS
3.2.4.2
3.2.4.3
3.3
References
4
Theo
Sjoerd van
and Aad Vollebregt
4.1
4.2
Custom Manufacturing
4.3
(UV/Vis and Fluorescence)
4.3.1
of the Product Itself
4.3.1.1
Aminotransferase Gene
4.3.2
of Product
4.3.2.1
by o-Phthaldehyde/Sulfite Derivatization of Ammonia
4.3.2.2
of Ammonia via
4.3.2.3
Using Cu2+ as Sensor for
4.4
Assays
4.4.1
for Enzymatic Activity
Vili
4.4.1.1
4.4.1.2
4.4.1.3
4.4.1.4
for Amidase-containing Clones
4.4.1.5
Clone
4.4.1.6
towards a-Methylphenylglycine Amide
4.4.2
Activity
4.4.2.1
for Amidase-containing Clones
4.4.2.2
4.5
References
Part II Genetic Selection
5
Nicholas J. Turner
5.1
5.1.1
5.1.2
5.2
5.2.1
5.2.2
5.2.3
5.2.4
5.2.5
5.2.6
5.2.7
5.2.8
5.3
5.3.1
5.3.2
5.3.3
5.3.4
5.3.5
5.3.6
5.3.7
5.4
References
Contents
6
of Enzyme-encoding Genes
Amir Aharoni, Cintia Roodveldt, Andrew D. Griffiths,
and Dan S. Tawfik
6.1
6.2
6.3
6.4
6.5
6.6
6.6.1
6.6.2
6.6.3
Recent Examples
6.7
6.8
6.9
References
7
Scott Lefurgy and Virginia Cornish
7.1
7.2
7.2.1
7.2.2
7.2.3
7.2.4
7.3
7.3.1
7.3.2
7.3.2.1
7.3.2.2
7.3.2.3
7.3.2.4
7.3.3
7.3.3.1
7.3.3.2
7.3.3.3
7.3.3.4
7.4
7.4.1
7.4.2
7.4.2.1
7.4.2.2
X
7.4.2.3 Enzyme Library
7.4.2.4 General
7.4.3 Glycosynthase Evolution 210
7.4.3.1 Rationale 211
7.4.3.2
7.4.3.3 Glycosynthase
7.4.3.4
7.4.3.5 General
7.5
References
8
Valeria Ma ia
8.1
8.2
in Cultivated Microorganisms
8.2.1
8.2.2
Approaches
8.3
and Enzymes
8.3.1
Concept
8.3.2
8.3.3
Strategy, Enrichment for Specific Genomes and Application
of High-throughput Screening Methods
8.3.4
8.4
References
Part III Enzyme Fingerprinting
9
Ruth Bimer-Crünberger, Hannes
Hubert Scholze, and
9.1
9.2
9.2.1
9.2.2
9.2.3
9.2.4
9.2.5
9.3
and Functional Enzyme Fingerprinting
Contents
9.3.1 Lipase and Esterase
9.3.1.1 Microbial Lipases and
9.3.1.2
9.3.1.3 Hormone-sensitive Lipase 257
9.3.2
9.3.3
9.3.3.1
and Cell Lines
References
TO Fingerprinting Methods for Hydrolases
Johann Crognux
10.1
10.1.1
10.1.2
10.1.3
10.2
10.2.1
10.2.2
10.2.3
10.3
10.3.1
10.3.2
10.3.3
10.3.4
10.4
References
11
Jennifer L Harris
11.1
11.2
11.2.1
11.2.2
of Peptide Libraries
11.2.3
Specificity
11.3
11.3.1
Substrates
11.3.2
11.4
References
XII
12 Enzyme
Souvik Chattopadhaya and Shao Q. Yao
12.1
12.2
12.2.1 Covalent versus Noncovalent
12.2.2
12.2.3
12.2.4
12.2.4.1
12.2.4.2
12.2.4.3
12.3
12.3.1
12.3.2
Substrate Arrays
12.3.2.1
12.3.2.2
12.3.2.3
12.3.2.4
12.3.3
12.4
References
Subject Index
|
| adam_txt |
Contents
Preface XIII
List of Contributors XV
Introduction
Renaud Sicard
Enzyme Assays
Part I: The Chemistry of Enzyme Assays
Part II: Enzyme Assays and Genetic Selection
Part III: Enzyme Profiling
Enzyme Assays in Other Areas
How to Use this Book
Part I High-throughput Screening
1
Using Color Changes
Romas J. Kazlauskas
1.1
1.2
1.3
1.3.1
1.3.2
1.3.2.1
(Testing Many Hydrolases Toward One Substrate)
1.3.2.2
(Testing Many Substrates Toward Hydrolase)
1.3.3
1.4
1.4.1
1.4.2
(Quick
Enzyme Assays: High-throughput Screening, Genetic Selection and Fingerprinting
Edited by Jean-Louis Reymond
Copyright
ISBN:
VI
1.4.2.1 Chromogenic Substrate 32
1.4.2.2 pH
1.4.3 Application 33
1.4.3.1 Substrate
1.4.3.2
IAA
References
2
the Enantioselectivity of Enzymes
Manfred T. Reetz
2.1
2.2
2.2.1
of Chiral p-Nitrophenyl Esters
2.2.2
2.2.3
(EMDee)
2.2.4
Enantiomeric Excess
2.2.5
2.3
2.3.1
2.3.2
2.3.3
2.4
2.4.1
2.5
2.6
for Assaying Lipases or
2.7
2.8
2.9
2.10
2.11
2.12
References
3
for Oxidoreductases
Tyler W. Johannes, Ryan D.
3.1
3.2
3.2.1
Contents
3.2.1.1
3.2.1.2
3.2.2
3.2.2.1
3.2.2.2
3.2.2.3
3.2.3
3.2.3.1
3.2.3.2
3.2.3.3
3.2.3.4
3.2.3.5
3.2.4
3.2.4.1 ABTS
3.2.4.2
3.2.4.3
3.3
References
4
Theo
Sjoerd van
and Aad Vollebregt
4.1
4.2
Custom Manufacturing
4.3
(UV/Vis and Fluorescence)
4.3.1
of the Product Itself
4.3.1.1
Aminotransferase Gene
4.3.2
of Product
4.3.2.1
by o-Phthaldehyde/Sulfite Derivatization of Ammonia
4.3.2.2
of Ammonia via
4.3.2.3
Using Cu2+ as Sensor for
4.4
Assays
4.4.1
for Enzymatic Activity
Vili
4.4.1.1
4.4.1.2
4.4.1.3
4.4.1.4
for Amidase-containing Clones
4.4.1.5
Clone
4.4.1.6
towards a-Methylphenylglycine Amide
4.4.2
Activity
4.4.2.1
for Amidase-containing Clones
4.4.2.2
4.5
References
Part II Genetic Selection
5
Nicholas J. Turner
5.1
5.1.1
5.1.2
5.2
5.2.1
5.2.2
5.2.3
5.2.4
5.2.5
5.2.6
5.2.7
5.2.8
5.3
5.3.1
5.3.2
5.3.3
5.3.4
5.3.5
5.3.6
5.3.7
5.4
References
Contents
6
of Enzyme-encoding Genes
Amir Aharoni, Cintia Roodveldt, Andrew D. Griffiths,
and Dan S. Tawfik
6.1
6.2
6.3
6.4
6.5
6.6
6.6.1
6.6.2
6.6.3
Recent Examples
6.7
6.8
6.9
References
7
Scott Lefurgy and Virginia Cornish
7.1
7.2
7.2.1
7.2.2
7.2.3
7.2.4
7.3
7.3.1
7.3.2
7.3.2.1
7.3.2.2
7.3.2.3
7.3.2.4
7.3.3
7.3.3.1
7.3.3.2
7.3.3.3
7.3.3.4
7.4
7.4.1
7.4.2
7.4.2.1
7.4.2.2
X
7.4.2.3 Enzyme Library
7.4.2.4 General
7.4.3 Glycosynthase Evolution 210
7.4.3.1 Rationale 211
7.4.3.2
7.4.3.3 Glycosynthase
7.4.3.4
7.4.3.5 General
7.5
References
8
Valeria Ma'ia
8.1
8.2
in Cultivated Microorganisms
8.2.1
8.2.2
Approaches
8.3
and Enzymes
8.3.1
Concept
8.3.2
8.3.3
Strategy, Enrichment for Specific Genomes and Application
of High-throughput Screening Methods
8.3.4
8.4
References
Part III Enzyme Fingerprinting
9
Ruth Bimer-Crünberger, Hannes
Hubert Scholze, and
9.1
9.2
9.2.1
9.2.2
9.2.3
9.2.4
9.2.5
9.3
and Functional Enzyme Fingerprinting
Contents
9.3.1 Lipase and Esterase
9.3.1.1 Microbial Lipases and
9.3.1.2
9.3.1.3 Hormone-sensitive Lipase 257
9.3.2
9.3.3
9.3.3.1
and Cell Lines
References
TO Fingerprinting Methods for Hydrolases
Johann Crognux
10.1
10.1.1
10.1.2
10.1.3
10.2
10.2.1
10.2.2
10.2.3
10.3
10.3.1
10.3.2
10.3.3
10.3.4
10.4
References
11
Jennifer L Harris
11.1
11.2
11.2.1
11.2.2
of Peptide Libraries
11.2.3
Specificity
11.3
11.3.1
Substrates
11.3.2
11.4
References
XII
12 Enzyme
Souvik Chattopadhaya and Shao Q. Yao
12.1
12.2
12.2.1 Covalent versus Noncovalent
12.2.2
12.2.3
12.2.4
12.2.4.1
12.2.4.2
12.2.4.3
12.3
12.3.1
12.3.2
Substrate Arrays
12.3.2.1
12.3.2.2
12.3.2.3
12.3.2.4
12.3.3
12.4
References
Subject Index |
| any_adam_object | 1 |
| any_adam_object_boolean | 1 |
| author_GND | (DE-588)130585467 |
| building | Verbundindex |
| bvnumber | BV020849991 |
| callnumber-first | Q - Science |
| callnumber-label | QP601 |
| callnumber-raw | QP601 |
| callnumber-search | QP601 |
| callnumber-sort | QP 3601 |
| callnumber-subject | QP - Physiology |
| classification_rvk | WC 4350 |
| classification_tum | CHE 828f |
| ctrlnum | (OCoLC)254486013 (DE-599)BVBBV020849991 |
| dewey-full | 572.76 |
| dewey-hundreds | 500 - Natural sciences and mathematics |
| dewey-ones | 572 - Biochemistry |
| dewey-raw | 572.76 |
| dewey-search | 572.76 |
| dewey-sort | 3572.76 |
| dewey-tens | 570 - Biology |
| discipline | Biologie Chemie Medizin |
| discipline_str_mv | Biologie Chemie Medizin |
| format | Book |
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| id | DE-604.BV020849991 |
| illustrated | Illustrated |
| index_date | 2024-07-02T13:19:31Z |
| indexdate | 2024-07-09T20:26:36Z |
| institution | BVB |
| isbn | 3527310959 9783527310951 |
| language | English |
| oai_aleph_id | oai:aleph.bib-bvb.de:BVB01-014171716 |
| oclc_num | 254486013 |
| open_access_boolean | |
| owner | DE-91G DE-BY-TUM DE-M49 DE-BY-TUM DE-20 DE-355 DE-BY-UBR DE-29T DE-92 DE-1102 DE-91S DE-BY-TUM DE-526 |
| owner_facet | DE-91G DE-BY-TUM DE-M49 DE-BY-TUM DE-20 DE-355 DE-BY-UBR DE-29T DE-92 DE-1102 DE-91S DE-BY-TUM DE-526 |
| physical | XVIII, 368 S. Ill., graph. Darst. |
| publishDate | 2006 |
| publishDateSearch | 2006 |
| publishDateSort | 2006 |
| publisher | WILEY-VCH |
| record_format | marc |
| spelling | Enzyme assays high-throughput screening, genetic selection and fingerprinting ed. by Jean-Louis Reymond Weinheim WILEY-VCH 2006 XVIII, 368 S. Ill., graph. Darst. txt rdacontent n rdamedia nc rdacarrier Enzym - Screening Enzymes Analysis Screening (DE-588)4054045-5 gnd rswk-swf Enzym (DE-588)4014988-2 gnd rswk-swf Chemische Analyse (DE-588)4009840-0 gnd rswk-swf Enzym (DE-588)4014988-2 s Screening (DE-588)4054045-5 s DE-604 Chemische Analyse (DE-588)4009840-0 s b DE-604 Reymond, Jean-Louis Sonstige (DE-588)130585467 oth Digitalisierung UBRegensburg application/pdf http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014171716&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA Inhaltsverzeichnis |
| spellingShingle | Enzyme assays high-throughput screening, genetic selection and fingerprinting Enzym - Screening Enzymes Analysis Screening (DE-588)4054045-5 gnd Enzym (DE-588)4014988-2 gnd Chemische Analyse (DE-588)4009840-0 gnd |
| subject_GND | (DE-588)4054045-5 (DE-588)4014988-2 (DE-588)4009840-0 |
| title | Enzyme assays high-throughput screening, genetic selection and fingerprinting |
| title_auth | Enzyme assays high-throughput screening, genetic selection and fingerprinting |
| title_exact_search | Enzyme assays high-throughput screening, genetic selection and fingerprinting |
| title_exact_search_txtP | Enzyme assays high-throughput screening, genetic selection and fingerprinting |
| title_full | Enzyme assays high-throughput screening, genetic selection and fingerprinting ed. by Jean-Louis Reymond |
| title_fullStr | Enzyme assays high-throughput screening, genetic selection and fingerprinting ed. by Jean-Louis Reymond |
| title_full_unstemmed | Enzyme assays high-throughput screening, genetic selection and fingerprinting ed. by Jean-Louis Reymond |
| title_short | Enzyme assays |
| title_sort | enzyme assays high throughput screening genetic selection and fingerprinting |
| title_sub | high-throughput screening, genetic selection and fingerprinting |
| topic | Enzym - Screening Enzymes Analysis Screening (DE-588)4054045-5 gnd Enzym (DE-588)4014988-2 gnd Chemische Analyse (DE-588)4009840-0 gnd |
| topic_facet | Enzym - Screening Enzymes Analysis Screening Enzym Chemische Analyse |
| url | http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&local_base=BVB01&doc_number=014171716&sequence=000001&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA |
| work_keys_str_mv | AT reymondjeanlouis enzymeassayshighthroughputscreeninggeneticselectionandfingerprinting |